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Basso A.,Purolite | Hesseler M.,GSK | Serban S.,Purolite
Tetrahedron | Year: 2016

A novel family of spherical beads made of octadecyl (OD) functionalised methacrylic resins for lipase immobilization was developed. The activity of the immobilized lipase reflected the effect of OD functional groups density (0.3-1.48 mmol OD/gdry) on the hydrophobic microenvironment. The immobilized enzyme activity on Lifetech ECR8806 was outstanding: lipase from Candida antarctica B (CALB) showed activity higher than 15,000 PLU/gdry. A significantly high activity was also observed for immobilized lipases from Rhizomucor miehi (RM) and Thermomices lanuginosus (TL), confirming the positive effect provided by the OD functional groups grafted on the methacrylic polymer backbone. © 2016 Elsevier Ltd.

Boodoo F.,Purolite | Windsor B.,Purolite | Lenormand G.,Purolite
Eau, l'INDUSTRIE, les Nuisances | Year: 2011

Specific resins Purolile SST 60 and Purolite MPR1000 used as pre-treatment before reverse osmosis units effectively eliminate minerals and fouling organic compounds. These resins can strongly enhance recovery yields and reduce -sometimes tremendously- rejects and water losses.

Basso A.,Purolite | Froment L.,Purolite | Hesseler M.,Purolite | Serban S.,Purolite
European Journal of Lipid Science and Technology | Year: 2013

Candida antartica lipase B (CALB) is the most versatile biocatalyst and is, therefore, widely used in industry. Its advantages range from thermostability, stereoselectivity, and activity in organic solvents to the acceptance of nucleophiles other than water. The actual standard for industrial applications of CALB is the immobilized enzyme on Lewatit VP OC 1600 and is marketed under the name Novozym® 435. A new acrylic enzyme carrier (ECR1030) for the immobilization by adsorption of CALB and for use in batch or column reactors has been developed. The resin shows an activity of >9000PLU/gDry, which is similar to Novozym® 435. Protein loading was 24.4mg/gWet, and immobilizations yields higher than 90% were obtained. Unlike the resin used for the production of Novozym® 435, ECR1030 possesses high mechanical stability and is therefore more suitable for applications that expose the polymers to high mechanical stress, such as multiple reuses in stirred reactors. Practical applications: Immobilized CALB is the biocatalyst used in the widest amount of industrial applications due to its broad selectivity and stability. It can be used for the manufacture of specialty chemicals such as surfactants, amides, chiral amines, alcohols, biodiesel, and polyesters. Its industrial use is in stirred tanks or packed columns, so high mechanical stability is needed together with high activity and sustainable costs. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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