Punjab Institute of Nuclear Medicine PINUM

Faisalābād, Pakistan

Punjab Institute of Nuclear Medicine PINUM

Faisalābād, Pakistan
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Shakoor M.B.,University of Agriculture at Faisalabad | Niazi N.K.,University of Agriculture at Faisalabad | Niazi N.K.,Southern Cross University of Australia | Bibi I.,University of Agriculture at Faisalabad | And 12 more authors.
Critical Reviews in Environmental Science and Technology | Year: 2015

Arsenic (As) contamination of groundwater reservoirs is a global environmental and health issue given to its toxic and carcinogenic nature. Over 170 million people have been affected by As due to the ingestion of As-contaminated groundwater. Conventional methods such as reverse osmosis, ion exchange, and electrodialysis are commonly used for the remediation of As-contaminated water; however, the high cost and sludge production put limitations on their application to remove As from water. This review critically addresses the use of various agricultural waste materials (e.g., sugarcane bagasse, peels of various fruits, wheat straw) as biosorbents, thereby offering an eco-friendly and low-cost solution for the removal of As from contaminated water supplies. The effect of solution chemistry such as solution pH, cations, anions, organic ligands, and various other factors (e.g., temperature, contact time, sorbent dose) on As biosorption, and safe disposal methods for As-loaded biosorbents to reduce secondary As contamination are also discussed. © 2016 Taylor & Francis Group, LLC


Ali F.,University of Agriculture at Faisalabad | Ali F.,Punjab Institute of Nuclear Medicine PINUM | Ahsan M.,University of Agriculture at Faisalabad | Saeed N.A.,National Institute for Biotechnology and Genetic Engineering NIBGE | And 7 more authors.
International Journal of Agriculture and Biology | Year: 2014

Two experiments were conducted to develop an efficient maize regeneration system using mature and immature embryos of four maize elite lines (Agaiti-85, Golden, Soneri and Sultan). The comparative studies revealed that all maize lines were responsive to callus and plant regeneration from mature and immature embryos. The culture medium Chu's N6 was used in both experiments. Immature embryos efficiently produced callus and regenerated plants therefore, when cultured on medium supplemented with 2,4-D, kinetin, 6-BAP and IBA at concentrations of 2 mL L-1, 0.2, 0.2 and 0.3 g L-1, respectively. However, callus-to-plant regeneration system for mature embryos required these growth regulators at higher concentrations (5 mL L-1, 0.4 g L-1, 0.5 g L-1 and 0.6 g L-1, respectively). The regeneration frequencies ranged from 40 to 75% using immature embryos, compared to 55 to 80% from immature embryos of all elite lines of maize under study. © 2014 Friends Science Publishers.


Ahmad L.,University of Agriculture at Faisalabad | Khan A.,University of Agriculture at Faisalabad | Khan M.Z.,University of Agriculture at Faisalabad | Hussain I.,University of Agriculture at Faisalabad | And 4 more authors.
Pesticide Biochemistry and Physiology | Year: 2012

The objective of this work was to identify the reproductive toxico-pathological effects of cypermethrin (CY) in mature male rabbits. Apparently healthy adult age- and weight-matched rabbits (n=40) were procured from the local market, kept under similar management conditions and divided into four equal groups. Different doses of CY (50, 100 and 150mgkg -1 body mass) mixed in mustard oil were injected intraperitonealy at weekly intervals in male rabbits (groups B-D) prior to mating. Group A served as control and each animal in this group received equivalent volume of mustard oil. Treatment was continued for 71days. Decreased testicular and epididymal sperm counts were recorded mostly in dose and time dependent manner in CY-treated rabbits. The serum testosterone concentrations in CY-treated rabbits were significantly lower than those of control group. Degeneration, arrested spermatogenesis and connective tissue (CT) proliferation in testes, while sperm-less seminal plasma and tailless spermatozoa in epididymis of CY-treated rabbits were observed. It was inferred that CY-induced defects in sperms and pathological alterations in testes and epididymis. © 2012 Elsevier Inc..


Akhtar M.S.,Punjab Institute of Nuclear Medicine PINUM | Imran M.B.,Punjab Institute of Nuclear Medicine PINUM | Nadeem M.A.,Punjab Institute of Nuclear Medicine PINUM | Shahid A.,Punjab Institute of Nuclear Medicine PINUM
International Journal of Peptides | Year: 2012

Nuclear medicine imaging techniques offer whole body imaging for localization of number and site of infective foci inspite of limitation of spatial resolution. The innate human immune system contains a large member of important elements including antimicrobial peptides to combat any form of infection. However, development of antibiotics against bacteria progressed rapidly and gained popularity over antimicrobial peptides but even powerful antimicrobials failed to reduce morbidity and mortality due to emergence of mutant strains of bacteria resulting in antimicrobial resistance. Differentiation between infection and inflammation using radiolabeled compounds with nuclear medicine techniques has always been a dilemma which is still to be resolved. Starting from nonspecific tracers to specific radiolabeled tracers, the question is still unanswered. Specific radiolabeled tracers included antibiotics and antimicrobial peptides which bind directly to the bacteria for efficient localization with advanced nuclear medicine equipments. However, there are merits and demerits attributed to each. In the current paper, radiolabeled antibiotics and radiolabeled peptides for infection localization have been discussed starting with the background of primitive nonspecific tracers. Radiolabeled antimicrobial peptides have certain merits compared with labeled antibiotics which make them superior agents for localization of infective focus. Copyright © 2012 Muammad Saeed Akhtar et al.


Imran M.B.,Punjab Institute of Nuclear Medicine PINUM | Naeem M.,Punjab Institute of Nuclear Medicine PINUM
Rheumatology International | Year: 2013

This is a case report of a young physically active patient who presented with pain in the right leg. Plain radiograph and bone scan showed lesions mimicking stress fracture. Follow-up investigations (repeated plain radiography and three phase bone scan) and histopathological examination proved it as a case of multifocal adamantinoma. This report signifies that physicians should not lock their mind only to one entity and keep the differential diagnosis list flexible especially if patient does not respond to a specific treatment. Tibial adamantinoma should also be considered in the differential diagnosis of stress fracture. Further, second lesion can occur in the rib cage defying the common belief that second lesion will be in the adjacent bone, fibula. © 2010 Springer-Verlag.


Amin I.,Punjab Institute of Nuclear Medicine PINUM | Jabbar T.,Punjab Institute of Nuclear Medicine PINUM | Niazi F.,Punjab Institute of Nuclear Medicine PINUM | Akhtar M.S.,Punjab Institute of Nuclear Medicine PINUM
International Journal of Analytical Chemistry | Year: 2015

A semiautomated extraction protocol of HCV-RNA using Favorgen RNA extraction kit has been developed. The kit provided protocol was modified by replacing manual spin steps with vacuum filtration. The assay performance was evaluated by real-time qPCR based on Taqman technology. Assay linearity was confirmed with the serial dilutions of RTA (Turkey) containing 1 × (106, 105, 104, and 103) IU mL-1. Comparison of test results obtained by two extraction methods showed a good correlation (r=0.95, n=30) with detection limit of 102 IU mL-1. The semiautomated vacuum filtration based protocol demonstrated high throughput: 35 minutes for the extraction of a batch of 30 samples (150 μL each) with reduced labor, time, waste, and cost. Performance characteristics of semiautomated system make it suitable for use in diagnostic purpose and viral load determinations. © 2015 Imran Amin et al.


PubMed | Punjab Institute of Nuclear Medicine PINUM
Type: | Journal: International journal of analytical chemistry | Year: 2015

A semiautomated extraction protocol of HCV-RNA using Favorgen RNA extraction kit has been developed. The kit provided protocol was modified by replacing manual spin steps with vacuum filtration. The assay performance was evaluated by real-time qPCR based on Taqman technology. Assay linearity was confirmed with the serial dilutions of RTA (Turkey) containing 1 (10(6), 10(5), 10(4), and 10(3)) IUmL(-1). Comparison of test results obtained by two extraction methods showed a good correlation (r = 0.95, n = 30) with detection limit of 10(2)IUmL(-1). The semiautomated vacuum filtration based protocol demonstrated high throughput: 35 minutes for the extraction of a batch of 30 samples (150L each) with reduced labor, time, waste, and cost. Performance characteristics of semiautomated system make it suitable for use in diagnostic purpose and viral load determinations.


PubMed | Punjab Institute of Nuclear Medicine PINUM
Type: | Journal: International journal of analytical chemistry | Year: 2016

[This corrects the article DOI: 10.1155/2015/367801.].


PubMed | Punjab Institute of Nuclear Medicine PINUM
Type: | Journal: International journal of peptides | Year: 2012

Nuclear medicine imaging techniques offer whole body imaging for localization of number and site of infective foci inspite of limitation of spatial resolution. The innate human immune system contains a large member of important elements including antimicrobial peptides to combat any form of infection. However, development of antibiotics against bacteria progressed rapidly and gained popularity over antimicrobial peptides but even powerful antimicrobials failed to reduce morbidity and mortality due to emergence of mutant strains of bacteria resulting in antimicrobial resistance. Differentiation between infection and inflammation using radiolabeled compounds with nuclear medicine techniques has always been a dilemma which is still to be resolved. Starting from nonspecific tracers to specific radiolabeled tracers, the question is still unanswered. Specific radiolabeled tracers included antibiotics and antimicrobial peptides which bind directly to the bacteria for efficient localization with advanced nuclear medicine equipments. However, there are merits and demerits attributed to each. In the current paper, radiolabeled antibiotics and radiolabeled peptides for infection localization have been discussed starting with the background of primitive nonspecific tracers. Radiolabeled antimicrobial peptides have certain merits compared with labeled antibiotics which make them superior agents for localization of infective focus.

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