Puning Peoples Hospital

Puning, China

Puning Peoples Hospital

Puning, China
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Wei L.,Affiliated Hospital of Guangdong Medical University | Zhou L.,Affiliated Hospital of Guangdong Medical University | Zhi-En X.,Affiliated Hospital of Guangdong Medical University | Li-Feng L.,Affiliated Hospital of Guangdong Medical University | Hong-Ming F.,Puning Peoples Hospital
Chinese Journal of Tissue Engineering Research | Year: 2016

BACKGROUND: It is unclear whether serial cell passage in vitro influences the differentiation of bone marrow mesenchymal stem cells into neural stem cells. OBJECTIVE: To investigate the effect of cell passage on the differentiation of bone marrow mesenchymal stem cells into neural stem cells. METHODS: Rat bone marrow mesenchymal stem cells were isolated and cultured by the whole bone marrow adherence method. Bone marrow mesenchymal stem cells at passages 3, 6, 9, 12 were incubated in serum-free medium. After culture for 7 and 14 days, cell biological characterization was observed and differenitaiton ability into neural stem cells was observed by detecting Nestin expression in cells using flow cytometry. Then, the cells were further induced to differentiate and cell multipotential differentiation capacity was detected by measurement of nerve enolase and glial acidic protein expression. RESULTS AND CONCLUSION: Under induction, bone marrow mesenchymal stem cells at different passages were all differentiated into Nestin-positive neural stem cells. However, there was a significant difference in differentiation proportion of cells at different passages (P < 0.05). Strongest differentiation ability was found in the passage 6 cells, with the Nestin expression up to (93.7±2.3)% at 7 days of induction and (96.2±1.8)% at 14 days of induction. The proportion of differentiated cells at passages 6 and 9 was signfiicantly higher than that at passages 3 and 12. Moreover, adherent cells were positive for nerve enolase and glial acidic protein. All these findings indicate that the differentiation of bone marrow mesenchymal stem cells into neural stem cells is correlated with cell passage. Cells at lower or higher passages are both detrimental to cell differentiation. © 2016, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.


Zhou Y.,Guangzhou University | Gurioli A.,University of Turin | Luo J.,Guangzhou University | Li Z.,Guangzhou University | And 3 more authors.
Journal of Endourology | Year: 2017

Objectives: To observe serum creatinine (SCr) and treated side glomerular filtration rate (TGFR) variations in patients with upper urinary tract calculi after minimally invasive percutaneous nephrolithotomy (MPCNL). Patients and Methods: A total of 178 patients underwent MPCNL in our institute and they were retrospectively evaluated between May 2014 and February 2016. SCr and TGFR variations were observed with renal scintigraphy using 99mTc-diethylene triamine pentaacetic acid (99mTc-DTPA) preoperatively and after at least 6 months of follow-up (FU). The patients were categorized into two groups according to the number of percutaneous access tracts: Group I (single tract, n = 122) and group II (multiple tracts, n = 56). Results: At a mean FU of 7.6 months, SCr dropped from 192.9 ± 151.9/L to 167.6 ± 113.9 μM (13.15% decrease, p = 0.008) and TGFR increased from 29.8 ± 21.2 mL/minute preoperatively to 32.7 ± 22.5 mL/minute postoperatively (9.79% increase, p = 0.022) in group I. Similarly, SCr dropped from 238.5 ± 130.1 to 215.8 ± 128.1μ (9.50% decrease, p = 0.013) and TGFR increased from 29.6 ± 21.4 mL/minute preoperatively to 32.9 ± 25.1 mL/minute postoperatively (11.17% increase, p = 0.014) in group II. No statistically significant difference between two groups according to SCr or TGFR variation was observed (p > 0.05). Conclusions: Stone clearance resulted in improvement of split kidney function after single tract or multiple tract MPCNL. Single tract or multiple tract MPCNL did not show statistically significant difference in split renal function postoperative recovery. © Mary Ann Liebert, Inc. 2017.


Fang X.-S.,Puning Peoples Hospital | Yang H.-C.,Puning Peoples Hospital | Yu J.-K.,Puning Peoples Hospital | Huang B.-J.,Puning Peoples Hospital
International Eye Science | Year: 2015

AIM: To compare the anti-inflammatory effects with lotrprednol-tobramycin and tobramysin-dexamethasone eyedrops after phacoemulsification combined intraocular lens implantation. METHODS: In this prospective, comparative, randomized controlled trial, patients with senile cataract who underwent phacoemulsification combined intraocular lens implantation in our hospital from January 2014 to October 2014 were enrolled, which were randomly divided into two groups. Patients were given lotrprednol-tobramycin (LE/T group) and dexamethasone-tobramysin (D/T group) eyedrops respectively. Preoperative and postoperative intraocular pressure, malnutrition inflammation score, bruggrmann comfort scale were observed. RESULTS: A total of 143 eyes of 143 cases were enrolled, of them 81 cases were in the LE/T group and 62 in the D/T group. There were no statistically significant differences between the two groups in malnutrition inflammation score and bruggrmann comfort scale. The average intraocular pressure was 16.5±3.1mmHg in D/T group after 14d postoperation, which was obviously higher than LE/T group (14.7±3.7mmHg, P=0.004). After 14d postoperation, the intraocular pressure of 2 cases were >21mmHg, which were back to normal after drug withdrawal. CONCLUSION: The anti-inflammatory effects lotrprednol-tobramycin and tobramysin-dexamethasone eyedrops are similar after phacoemulsification. However, lotrprednol-tobramycin has little influence in intraocular pressure, and more safety. Copyright 2015 by the IJO Press.


Wei Y.-T.,Sun Yat Sen University | Li Y.-Q.,Sun Yat Sen University | Bai Y.-J.,Sun Yat Sen University | Wang M.,Sun Yat Sen University | And 3 more authors.
Molecular Vision | Year: 2011

Purpose: To investigate the genotype and phenotype of juvenile-onset open angle glaucoma (JOAG) in a Chinese family (PN pedigree). Methods: Each family member was comprehensively examined by an experienced ophthalmologist. The clinical characteristics of the family patients with JOAG were documented. Blood samples were obtained from 22 available participants from the PN pedigree. Linkage analysis was performed to identify the possible chromosome loci. The presence of gene mutation was ascertained by polymerase chain reaction amplification and subsequent direct sequencing. Results: The affected members in the PN pedigree are characterized by early age of onset (mean age at diagnosis is 17 years old), severe clinical presentations, high intraocular pressure (mean IOP of 34.18±2.97 mmHg), and poor response to pharmacological treatment (87.5% of the patients required filtering surgery). The region on chromosome 1 between D1S3464 and D1S1619 was identified in this pedigree by linkage analysis. A Pro370Leu myocilin mutation resulting from a heterozygous C→T transition at the 1,109th nucleotide in exon 3 was detected by gene sequencing. The Pro370Leu mutation co-segregated among all affected individuals of PN pedigree. Conclusions: The GLC1A Pro370Leu mutation is firmly correlated with a severe POAG phenotype. These data provide clues for the severe disease-causing nature of the Pro370Leu allele. Gene screening may be a useful method for presymptom diagnosis and a forewarning to detect the at-risk individuals in familial open-angle glaucoma patients, especially in pedigrees of early-onset. © 2011 Molecular Vision.


Su W.-K.,Puning Peoples Hospital | Ye S.-T.,Puning Peoples Hospital
Chinese Journal of Tissue Engineering Research | Year: 2015

BACKGROUND: The effects of posterior decompression fixation in the repair of thoracolumbar fracture are affirmative. The fixation was divided into short-segment and long-segment pedicle screw fixation according to different treatment methods. The therapeutic effects of above two methods are controversial in the clinic at present. OBJECTIVE: To compare and observe the anterior vertebral compression ratio and the changes in Cobb angle after posterior short-segment and long-segment pedicle screw placement fixation in the repair of thoracolumbar fracture. METHODS: 120 patients with thoracolumbar fracture were enrolled in this study, and assigned to two groups according to fixation methods. Study group received posterior short-segment pedicle screw fixation. Control group received posterior long-segment pedicle screw fixation. Anterior vertebral compression rate and Cobb angle were observed before fixation, immediately and 12 months after fixation in both groups. RESULTS AND CONCLUSION: There was no significant difference in anterior vertebral compression rate and Cobb angle between the two groups before fixation (P > 0.05). Anterior vertebral compression rate and Cobb angle were significantly smaller in the study group than in the control group immediately and 12 months after fixation (P < 0.05). Besides, surgical time and blood loss volume were less in the study group than in the control group (P < 0.05). These results indicated that posterior short-segment pedicle screw fixation for treatment of thoracolumbar fracture can effectively improve kyphosis, recover vertebral compression degree, and its surgical time is short and blood loss volume during surgery is little, so it is better than posterior long-segment pedicle screw fixation. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research , All Rights Reserved.


He W.,Shantou University | Ni S.,Puning Peoples Hospital | Chen G.,Shantou University | Jiang X.,Shantou University | Zheng B.,University of Alberta
Surgical Endoscopy and Other Interventional Techniques | Year: 2014

Background: Previous studies on surgical team composition have shown that surgical team size had an independent impact on surgical performance in US and Canadian hospitals. We aimed to investigate the impact of team composition on surgical performance in two Chinese hospitals. Methods: General surgery procedures performed from April 2011 to June 2012 were retrospectively reviewed to record the number of attendees in the operating room (OR) and the procedure time (PT). Results: A total of 1,900 valid procedures, mostly laparoscopic, were performed during the study period. The mean PT was 90.5 min. On average, there were a total of 6 (range = 3-8) team members per procedure: 3 (range = 1-5) surgeons, 2 nurses, and 1 anesthesiologist. Unlike the data reported for the US and Canada, the number of nurses and anesthesiologists remained stable in most cases, whereas the number of surgeons differed by procedure. Multiple-regression analysis revealed that both the complexity of the operation and the team size significantly affected PT. When procedure complexity and patient condition were kept constant, adding one team member in our data analysis predicted an increase of 34.7 min in the PT. Conclusion: The surgical team size has a measurable effect on PT. Aside from surgical complexity, the team composition and member stability affected PT in the OR. Optimizing surgical teams and developing a strategy to maintain team stability are of great importance for improving OR efficiency. © 2013 Springer Science+Business Media.


Fang X.-S.,Puning Peoples Hospital | Yang H.-C.,Puning Peoples Hospital | Yu J.-K.,Puning Peoples Hospital | Li C.-X.,Puning Peoples Hospital
International Eye Science | Year: 2015

AIM: To elevate the stability of Bigbag intraocular lens (IOL) implanted for high myopia. METHODS: In this prospective control trail, axial length ≥28mm of high myopia with or without cataract patients in our hospiotal from May 2012 to February 2014 were selected. They were divided into two groups and were implanted with Bigbag IOL or Sensar IOL after phacoemulsification surgery. After 6mo, complications and the status of IOL posterior capsule were observed, the differences of distance between posterior capsule and retina before and after operation (Δd) were compared. RESULTS:Sixty-three eyes of 52 cases were included. There were 30 eyes of 24 cases (female: 19 eyes of 15 cases; male: 11 eyes of 9 cases) in the Bigbag group and 33 eyes of 28 cases (female: 19 eyes of 16 cases; male: 14 eyes of 12 cases) in the Sensar group. The Δd was 1.49±0.06mm in the Bigbag group and 1.67±0.09mm in the Sensar group, with statistically significant difference (P<0.01). At 6mo postoperatively, posterior capsule striae (PCS) were found in 2 eyes (6.7%) of Bigbag group and 9 eyes (27.3%) of Sensar group, with statistically significant difference (P=0.031). CONCLUSION:The stability of Bigbag IOL is better than Sensar wiht low incidence of PCS. The Bigbag IOL is more suitable for high myopia. ©, 2015, International Journal of Ophthalmology (c/o Editorial Office). All right reserved.


Zhang J.-Z.,Puning Peoples Hospital | Chen J.-H.,Puning Peoples Hospital | Huang B.-J.,Puning Peoples Hospital | Jiang Y.,Puning Peoples Hospital | And 3 more authors.
International Eye Science | Year: 2014

AIM: To observe the effect of 1.8 mm coaxial micro-incision cataract phacoemulsification on corneal astigmatism, and compared with the effect of traditional 3.2 mm small incision cataract phacoemulsification. METHODS: Totally 160 patients (168 eyes) with age-related cataract were selected, whose age from 50 to 83 years old. Their lens opacities classification was II-V grade. Two groups were divided randomly and each group included 84 eyes. They performed 1.8 mm coaxial micro-incision (micro-vision group) and traditional 3.2 mm small incision (control group) of cataract phacoemulsification respectively. After 1d, 1 wk, 1 mo follow-up, their naked vision and astigmatism changes were recorded. The χ2-test and t-test was used for the statistical analysis. RESULTS: After 1d, 1 wk, 1 mo of the micro-incision surgery, naked vision ≥0.5 of the patients were 79%, 83% and 94% respectively. In control group, naked vision ≥0.5 were 46%, 64% and 85% respecticvely. There was significant difference between the two groups (P<0.05). The average corneal astigmatism of micro-incision group was 0.75±0.45D on 1d after surgery, and that of the control group was 1.12±0.55D. There were significant difference between two groups (P<0.01). After 1wk and 1mo, the average corneal astigmatism of micro incision group was 0.76±0.40D and 0.65±0.35D, and the average corneal astigmatism of control group was 1.05±0.53D and 0.85±0.43D. The two groups were statistically significant (P<0.05). CONCUSION: The corneal astigmatism of 1.8 mm coaxial micro-incision cataract phacoemulsification was smaller than that of the conventional phacoemulsification and visual recovery was also better after surgery.


PubMed | Puning Peoples Hospital, Guangzhou University, Citta della salute e della Science and Guizhou Provincial Peoples Hospital
Type: | Journal: Journal of endourology | Year: 2017

To observe serum creatinine (SCr) and treated side glomerular filtration rate (TGFR) variations in patients with upper urinary tract calculi after minimally invasive percutaneous nephrolithotomy (MPCNL).A total of 178 patients underwent MPCNL in our institute and they were retrospectively evaluated between May 2014 andFebruary 2016. SCr and TGFR variations were observed with renal scintigraphy using 99mTc-diethylene triamine pentaacetic acid 99mTc-DTPA preoperatively and after at least 6 months of follow-up (FU). The patients were categorized into two groups according to the number of percutaneous access tracts: group I (single tract, n=122) and group II (multiple tracts, n=56).At a mean FU of 7.6months, SCr dropped from 192.9151.9 umol/L to 167.6113.9 umol/L ( 13.15% decrease, p=0.008) and TGFR increased from 29.821.2 ml/min preoperatively to 32.722.5 ml/min postoperatively (9.79% increase, P=0.022 ) in group I. Similarly, SCr dropped from 238.5130.1 umol/L to 215.8128.1 umol/L ( 9.50% decrease, p=0.013) and TGFR increased from 29.621.4 ml/min preoperatively to 32.925.1 ml/min postoperatively (11.17% increase, P=0.014 ) in group II. No statistically significant difference between two groups according to SCr or TGFR variation was observed (P>0.05).Stone clearance resulted in improvement of split kidney function after single tract or multiple tracts MPCNL. Single tract or multiple tracts MPCNL didnt show statistically significant difference in split renal function postoperative recovery.

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