Pulmonary and Critical Care Medicine of PLA Navy General Hospital

Beijing, China

Pulmonary and Critical Care Medicine of PLA Navy General Hospital

Beijing, China
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Han Z.-H.,Pulmonary and Critical Care Medicine of PLA Navy General Hospital | Duan Y.-Y.,Pulmonary and Critical Care Medicine of PLA Navy General Hospital | Wang X.-Y.,Pulmonary and Critical Care Medicine of PLA Navy General Hospital | Huang Y.,Pulmonary and Critical Care Medicine of PLA Navy General Hospital | Fang T.-Z.,Pulmonary and Critical Care Medicine of PLA Navy General Hospital
Experimental and Therapeutic Medicine | Year: 2015

The aim of the present study was to investigate the mechanism by which hydrogen sulfide (H2S) inhalation protects against oxidative stress in rats with cotton smoke inhalation-induced lung injury. A total of 24 male Sprague-Dawley rats were separated randomly into four groups, which included the control, H2S, smoke and smoke + H2S groups. A rat model of cotton smoke inhalation-induced lung injury was established following inhalation of 30% oxygen for 6 h. In addition, H2S (80 ppm) was inhaled by the rats in the H2S and smoke + H2S groups for 6 h following smoke or sham-smoke inhalation. Enzyme-linked immunosorbent assays were performed to measure various indices in the rat lung homogenate, while the levels of nuclear factor (NF)-κBp65 in the lung tissue of the rats were determined and semiquantitatively analyzed using immunohistochemistry. In addition, quantitative fluorescence polymerase chain reaction was employed to detect the mRNA expression of inducible nitric oxide synthase (iNOS) in the rat lung tissue. The concentrations of malondialdehyde (MDA), nitric oxide (NO), inducible iNOS and NF-κBp65, as well as the sum-integrated optical density of NF-κBp65 and the relative mRNA expression of iNOS, in the rat lung tissue from the smoke + H2S group were significantly lower when compared with the smoke group. The concentrations of MDA, NO, iNOS and NF-κBp65 in the H2S group were comparable to that of the control group. Therefore, inhalation of 80 ppm H2S may reduce iNOS mRNA transcription and the production of iNOS and NO in rats by inhibiting NF-κBp65 activation, subsequently decreasing oxidative stress and cotton smoke inhalation-induced lung injury. © Spandidos Publications 2015. All rights reserved.


Zhang Y.,Pulmonary and Critical Care Medicine of PLA Navy General Hospital | Han Z.-H.,Pulmonary and Critical Care Medicine of PLA Navy General Hospital | Duan Y.-Y.,Pulmonary and Critical Care Medicine of PLA Navy General Hospital | Jiang Y.,Pulmonary and Critical Care Medicine of PLA Navy General Hospital | And 3 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2016

The study aimed to observe the effects of inhaled hydrogen sulfide on smoke inhalation-induced acute lung injury in an animal model. In this study, male Sprague-Dawley (SD) rats were divided into intervention and control groups. The intervention group was divided into three subgroups: smoke group (n=6), smoke+H2S 3 h group (n=6), and smoke+H2 S 6 h group (n=6). A control group (n=6) received treatment with fresh air only. After exposure to treatment conditions and blood gas analysis, intervention and control group rats were sacrificed and pathological changes in pulmonary tissue were observed under a light microscope. Enzyme-linked immunosorbent assay (ELISA) was used to analyze the levels of malondialdehyde (MDA), inducible nitric oxide synthase (iNOS), and the p65 subunit of nuclear factor kappaB (NF-kBp65) in homogenized lung tissue. The expression of iNOS mRNA in homogenized lung tissue was analyzed using fluorescence quantitative polymerase chain reaction (PCR). In the smoke+H2S 3 h and smoke+H2 S 6 h groups, the sum integrated optical density of NF-kBp65 and MDA, the relative expression of iNOS mRNA, and the concentrations of NF-kBp65, iNOS, and nitrogen monoxide (NO) in lung tissue decreased significantly compared to the smoke-only group. No significant differences in lung tissue pathology, blood oxygen analysis, lung wet-dry weight ratio, MDA level, and other indicators were present between the smoke+H2S 3 h group and smoke+H2 S 6 h group. Inhaled hydrogen sulfide shows a significant protective effect on cotton smoke inhalation-induced pulmonary injury, and it can ameliorate oxidative stress and tissue inflammation. © 2016, E-Century Publishing Corporation. All rights reserved.

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