Pulla Reddy Institute of Pharmacy

Medak, India

Pulla Reddy Institute of Pharmacy

Medak, India
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Ravali P.,Pulla Reddy Institute of Pharmacy
Asian Journal of Pharmaceutical and Clinical Research | Year: 2017

Objective: The aim of the present work was to investigate the in vitro cytotoxic activity of chloroform extract of Sida acuta Burm.f. Methods: The in vitro cytotoxic activity of chloroform extract was performed by 3-(4, 5–dimethyl thiazol–2–yl)–2, 5–diphenyl tetrazolium bromide assay method against A-431 (human epidermoid carcinoma) and HeLa (human cervical cancer) cell lines. Results: The various concentrations of crude chloroform extract (1000, 500, 250, 125, and 62.5 µg/ml concentration) of S. acuta whole plant were performed for cytotoxic activity. Effect of inhibition of cell growth showed significantly cytotoxic against A-431 cell lines (human epidermoid carcinoma) with an inhibit cell growth by 50% (IC50) of 375±0.00 and HeLa cell lines (human cervix carcinoma) with an IC50 of 610.00±2.5. The results obtained from the study indicate significant cytotoxic activity. The result of anticancer activity study in cell lines of the extract indicates that S. acuta has anticancer activity against A-431-human epidermoid carcinoma and HeLa-human cervical cancer cell lines. Conclusion: The present study concluded that the chloroform extract of S. acuta Burm.f. possess potent cytotoxic activity. © 2017 The Authors.

Patil S.S.,N E T Pharmacy College | Ram Mohan Gupta V.,Pulla Reddy Institute of Pharmacy | Srikanth Gupta K.,Pulla Reddy Institute of Pharmacy | Doddayya H.,N E T Pharmacy College
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2014

Objective: The objective of the present study is to investigate the effect of pH, selected cyclodextrins and methods of complexation on the solubility of lornoxicam.Methods: Phase solubility studies were carried out according to Higuchi and Connors. Inclusion complexes of lornoxicam were prepared by different methods like kneading, ultrasoncation, spray drying along with the physical mixtures using β cyclodextrin and hydroxypropyl β cyclodextrins.Results: Lornoxicam being weakly acidic drug showed extremely low solubility in the acidic medium (pH 1.2) and poor solubility in water. The solubility of the drug increased as the pH of the medium was subsequently increased up to 7.4 and a drastic increase in solubility perhaps several hundred folds was observed with the alkaline phosphate buffer (pH 10.0). Phase solubility studies revealed that, hydroxypropyl β cyclodextrin (HP β CD) up to the concentration of 20 mM showed a linear increase in solubility of lornoxicam whereas the solubility of lornoxicam was increased up to β cyclodextrin (β CD) concentration of 14 mM and beyond that the solubility of the drug reduced probably due to precipitation of the complexes. The stability constant (Ks) was found to be 378.55 M-1 and 867.262 M-1for β CD and HP β CD respectively. Inclusion complexes of lornoxicam with cyclodextrins were prepared employing different methods and the effect of complexation methods on the dissolution of lornoxicam was studied. Dissolution studies revealed that, irrespective of the cyclodextrins used (β CD and HP β CD), highest drug release rate was observed from the spray dried products compared to those prepared by kneading and ultrasonication methods. Inclusion complexes prepared using HP β CD showed higher drug release compared to those prepared using β CD.Conclusion: The study demonstrated the distinctive pH dependent of solubility of lornoxicam and also showed that cyclodextrins especially HP β CD can be utilized to improve the solubility of lornoxicam. © 2014, International Journal of Pharmacy and Pharmaceutical Sciences. All rights reserved.

Goswami N.,Dr. Reddys Laboratories Ltd. | Goswami N.,Singhania University | Gupta V.R.M.,Singhania University | Gupta V.R.M.,Pulla Reddy Institute of Pharmacy | And 2 more authors.
Scientia Pharmaceutica | Year: 2013

A stability-indicating reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed for the simultaneous determination of halometasone, fusidic acid, methylparaben, and propylparaben in topical pharmaceutical formulation. The desired chromatographic separation was achieved on an Agilent Zorbax CN (Cyano), 5 μm (250 × 4.6 mm) column using gradient elution at 240 nm detector wavelength. The optimized mobile phase consisted of a mixture of 0.01 M phosphate buffer and 0.1% orthophosphoric acid, pH-adjusted to 2.5 with an ammonia solution as solvent-A and acetonitrile as solvent-B. The developed method separated halometasone, fusidic acid, methylparaben, and propylparaben in the presence of known impurities/ degradation products. The stability-indicating capability was established by forced degradation experiments and separation of known and unknown degradation products. The developed RP-HPLC method was validated according to the International Conference on Harmonization (ICH) guidelines. This validated method was applied for the simultaneous estimation of HM, FA, MP, and PP in commercially available cream samples. Further, the method can be extended for the estimation of HM, FA, MP, and PP in various commercially available dosage forms. © Goswami et al.; licensee Österreichische Apotheker-Verlagsgesellschaft m. b. H., Vienna, Austria.

Velivela S.,Pulla Reddy Institute of Pharmacy | Vinyas M.,Pulla Reddy Institute of Pharmacy | Pati N.B.,Pulla Reddy Institute of Pharmacy
International Journal of Applied Pharmaceutics | Year: 2016

Objective: The purpose of this study was to formulate Ritonavir floating matrix tablets by melt granulation technique in order to prolong its gastric residence time thereby increasing its bioavailability. Methods: The Ritonavir tablets were prepared by melt granulation technique, using carriers such as Gelucire 43/01, Gelucire 50/02 and Geleol pellets alone or in combinations. Ethyl cellulose was used as drug release rate retarding agent and sodium bicarbonate was used as gas generating agent. The prepared granules were subjected to pre and post compressional parameters. The formulations were optimized on the basis of matrix integrity, duration of floating, swelling behavior and In vitro drug release. Results: The drug-excipients compatibility studies were performed by FT-IR and the study revealed that there is no drug excipients interaction. A combined matrix system containing Gelucire 43/01: Gelucire 50/02 with 1:1 concentration along with HPMC K 15 M shows good drug release pattern with less floating lag time and increased floating duration. Conclusion: The in vitro drug release pattern of Ritonavir floating tablets was fitted to different kinetic models which showed the highest regression for Zero order kinetics with Higuchi mechanism. © 2016 The Authors. Published by Innovare Academic Sciences Pvt Ltd.

Rama Mohan Gupta V.,Pulla Reddy Institute of Pharmacy
International Journal of Pharmaceutical Sciences Review and Research | Year: 2013

The present work is aimed to study the pharmacokinetic parameters including AUC0-t, AUC0-∞, Cmax, Tmax, T1/2 and elimination rate constant (Kel) were determined from plasma concentrations of zaltoprofen spherical agglomerated dense compacts by 24 factorial design followed by comparison with a commercially available formulation of zaltoprofen. Foremost spherical crystal of zaltoprofen prepared with PEG 6000 which is hydrophilic polymer and evaluated for different compressibility parameters and invitro dissolution studies, the best resulted compressed as dense compacts. Pharmacokinetic parameters of the compressed dense compacts were evaluated in rabbit models. Specific high performance liquid chromatographic method was used to determine drug plasma concentrations. The comparative pharmacokinetic results such as tmax, t1/2, AUC, MRT were increased significantly for the zaltoprofen in PEG 6000 spherical agglomerated dense compacts contrast with marketed product of zaltoprofen.

Sree Giri Prasad B.,P.A. College | Siva Subramanian N.,P.A. College | Swetha M.,P.A. College | Gupta V.R.M.,Pulla Reddy Institute of Pharmacy | And 2 more authors.
Der Pharmacia Lettre | Year: 2013

The purpose of the present research was to optimize the formulation of Orodispersible tablets of Levocetrizine. Orodispersible tablets of Levocetrizine were prepared by Melt Granulation Technology. The formulations were evaluated for Tablet weight variation, content uniformity, hardness, friability, wetting time, dispersion time, drug content and in vitro release also have been studied. All formulations showed satisfactory mechanical strength and tablets containing Crospovidone (10%) showed excellent in vitro dispersion time and drug release as compared to other formulations. The results revealed that the tablets containing 10% Crospovidone (F8) showed short dispersion time (12sec) with maximum drug release (100%) in 20min. FTIR & DSC results showed no evidence of interaction between the drug and polymers. This study helps in revealing the effect of formulation processing variables on tablet properties. It can be concluded that the Orodispersible tablets of Levocetrizine tablets could be prepared by Melt Granulation Technology using Crospovidone as superdisintegrant.

Sahoo S.,Pulla Reddy Institute of Pharmacy | Veliyath S.K.,Moonray Institute of Pharmaceutical science | Mahendra Kumar C.B.,St. Francis College
International Journal of Research in Pharmaceutical Sciences | Year: 2012

For the past two decades, modification of 1, 2, 4 triazole nucleus have made a tremendous significance in medicinal chemistry. 1, 2, 4 triazoles and their derivatives are found to have wide variety of pharmacological uses. From literature survey it is well known that triazole heterocycles exhibit manifold importance in the field of medicinal chemistry as a potent chemotherapeutic agent. Triazole is a synthetically versatile substrate used for the synthesis of a large variety of heterocyclic compounds, such as triazole fused with thiadiazole, oxadiazole and as a raw material for drug synthesis. Much work has been carried out on triazoles as potent anti fungal agents and many drugs with triazole nucleus having antifungal properties have come into the market (e.g. Fluconazole, Voriconazole, Itraconzole). This review represents the synthesized 1,2,4 triazole derivatives and their pharmacological profiles which may contribute in future to synthesize various analogs and to develop new pharmacologically less toxic medicines. © JK Welfare & Pharmascope Foundation.

Sahoo S.,Pulla Reddy Institute of Pharmacy | Mahendra Kumar C.B.,St. Mary's College
Asian Journal of Chemistry | Year: 2016

Based on the outcome of computational docking to the active site of cytochrome P450 14α-demethylase (CYP51), diverse 3,4-disubstituted 5-mercapto-1,2,4-triazoles were prepared and screened for antioxidant and antifungal activities. The docking study of synthesized compounds showed promising binding affinity towards docked enzyme, sterol 14α-demethylase(CYP51) from trypanosome cruzi obtained from a RCSB protein data bank (PDB ID: 3KHM). The synthesized compounds were characterized by IR, 1H NMR and Mass spectral data. Among the novel synthesized compounds IV-6, IV-1 and IV-2 showed maximum antifungal activity against A. Niger and C. albicans organism when compared the standard fluconazole. For antioxidant activity, all the compounds showed moderate activity but compound IV-6 and IV-7 showed significant activity when compared to standard ascorbic acid.

Rama Mohan Gupta V.,Pulla Reddy Institute of Pharmacy
Asian Journal of Pharmaceutical and Clinical Research | Year: 2012

The direct compression is a modern technique in the tablet manufacturing, many processing steps are limited in direct compression compared to conventional wet granulation method and also wet granulation cannot be used with sensitive drugs. Spherical agglomeration is a modern technique for development of directly compressible pharmaceutical dosage forms where the drug crystals are converted to spherical form to improve flowability, compressibility and packability. The spherical crystallization further developed use with hydrophilic polymers to enhance dissolution rate characteristics of poorly water soluble drugs. The spherical agglomerates evaluated in terms of flow properties, particle size analysis, compression and dissolution behavior. Physical characters of the crystals were studied for the morphology of crystals using scanning electron microscope (SEM), identification of polymorphism done by x-ray powder diffraction (XPRD) and for thermo dynamic properties using differential scanning calorimetry (DSC).

Patil P.B.,Net Pharmacy College | Gupta V.R.M.,Pulla Reddy Institute of Pharmacy | Udupi R.H.,Net Pharmacy College | Srikanth K.,Pulla Reddy Institute of Pharmacy | Prasad B.S.G.,Pulla Reddy Institute of Pharmacy
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2010

Mefenamic acid, a non steroidal anti-inflammatory drug is poorly water soluble. Addition of surfactant to the dissolution medium improves the dissolution of pure drug by facilitating the drug release process at the solid/liquid interface and micelle solubilization in the bulk. In the present study a dissolution medium was developed. The composition of the dissolution medium was selected on the basis of solubility data of Mefenamic acid at 37° C. The solubility data revealed that water consisting of 2% w/v sodium lauryl sulphate shall be suitable dissolution medium. The discriminating power of the selected dissolution medium (2% w/v sodium lauryl sulphate in water.) relative to the other dissolution medium was evaluated. The results further justified that the usage of 2% w/v sodium lauryl sulphate in water serves as most suitable dissolution medium for Mefenamic acid.

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