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Pudong, China

Liu B.-F.,Shanghai University | Zhang L.-G.,Tongji University | Liu Y.-B.,Shanghai University | Yan N.,Pudong Hospital | And 5 more authors.
Chinese Medical Journal | Year: 2011

Background Previous clinical and basic research of axial lumbar interbody fusion (AxiaLIF) all focused on the L5/S1. However, there is no data on the feasibility of this approach for the fusion of both L4/5 and L5/S1. This study aimed to explore whether transsacral axial interbody fusion is a candidate for the fusion of both L4/5 and L5/S1. Methods The subjects (n=40) underwent lumbosacral magnetic resonance imaging (MRI). The median sagittal MRI images were analyzed and five measurement markers were defined as follows: the center of the L4/5 disc (A), the center of the L5/S1 disc (B), the anterior margin of the S1/2 space (C), the sacrococcygeal junction (D), and the coccygeal tip (E). The measurement markers were connected each other to produce nine lines (AB, AC, AD, AE, BC, BD, BE, CD and CE) as the reference lines for surgical approaches. The distance between each reference line and the anterior and posterior margins of the L4, L5 and S1 vertebral bodies were measured to determine the safety of the respective approaches. Results Twenty subjects were capable of finding one reference line to fuse both L4/5 and L5/S1 via transsacral axial interbody fusion approach. The surgical approach reference line was AE or CE line. In the other 20 subjects, it was failed to find a reference line which met the safety criteria for fusing both L4/5 and L5/S1. Conclusions About half of subjects were capable of finding a suitable AxiaLIF reference line to fuse both L4/5 and L5/S1. In some subjects, it was difficult to find a suitable AxiaLIF reference line to fuse both L4/5 and L5/S1.

Yan X.,Fudan University | Yan X.,Chinese PLA General Hospital | Zhang T.,Fudan University | Wang Z.,Fudan University | And 6 more authors.
Journal of Genetics and Genomics | Year: 2011

Waardenburg syndrome type II (WS2) is associated with syndromic deafness. A subset of WS2, WS2A, accounting for approximately 15% of patients, is attributed to mutations in the microphthalmia-associated transcription factor (MITF) gene. We examined the genetic basis of WS2 in a large Chinese family. All 9 exons of the MITF gene, the single coding exon (exon 2) of the most common hereditary deafness gene GJB2 and the mitochondrial DNA (mtDNA) 12S rRNA were sequenced. A novel heterozygous mutation c.[742_743delAAinsT;746_747delCA] in exon 8 of the MITF gene co-segregates with WS2 in the family. The MITF mutation results in a premature termination codon and a truncated MITF protein with only 247 of the 419 wild type amino acids. The deaf proband had this MITF gene heterozygous mutation as well as a c.[109G>A]+[235delC] compound heterozygous pathogenic mutation in the GJB2 gene. No pathogenic mutation was found in mtDNA 12S rRNA in this family. Thus, a novel compound heterozygous mutation, c.[742_743delAAinsT;746_747delCA] in MITF exon 8 was the key genetic reason for WS2 in this family, and a digenic effect of MITF and GJB2 genes may contribute to deafness of the proband. © 2011.

Deng Q.-Q.,Jingan District Central Hospital | Gao L.-L.,Science and Technology Innovation Center | Fu J.-H.,Pudong Hospital | Pan D.-H.,Pudong Hospital | And 4 more authors.
Fudan University Journal of Medical Sciences | Year: 2015

Objective: To explore the prevalence and distribution characteristics of hyperhomocysteinemia (HHcy) of the elderly population in a rural community of Shanghai, and to analyze its related risk factors. Methods: A cross-sectional survey on the level and distribution characteristics of plasma homocysteine (Hcy) was conducted in the elderly (≥60 years) in Datuan community of Shanghai. Multi-factor non-conditional logistic regression was used to analyze the risk factors of Hcy level. Results: A total of 1621 elderly people were enrolled in this study. The plasma Hcy level in the male was higher than that in the female, and the difference was statistically significant [(15.72±9.22) μmol/L vs. (14.08±7.55) μmol/L, P<0.001], while the prevalence of HHcy in the male was higher than that in the female (43.6% vs. 29.5%, P<0.001). The plasma Hcy levels in the different age groups of <70 years, 70-80 years and >80 years were (13.75±5.43), (15.26±9.72) and (15.55±9.12) μmol/L (P=0.001), while the prevalences of HHcy were 23.9%, 40.5% and 46.3%, respectively (P<0.001). The plasma Hcy levels in the groups with and without smoke were (16.14±8.05) and (14.60±8.31) μmol/L (P=0.037), while the prevalences of HHcy were 44.9% and 34.2%, respectively (P=0.011). The plasma Hcy levels in the groups with and without hypertension were (15.24±9.72) and (14.20±6.42) μmol/L (P=0.012), while the prevalences of HHcy were 39.7% and 30.3%, respectively (P<0.001). The Logistic regression showed that gender, age and hypertension were risk factors for HHcy with the odds ratio (OR) of 1.92 (95%CI: 1.46-2.54, P<0.001), 1.89 (95%CI: 1.55-2.29, P<0.001) and 1.57 (95%CI: 1.20-2.05, P=0.001), respectively. Conclusions: Plasma Hcy level had difference in gender and ages in the 1621 elderly population in Datuan Community of Shanghai. The risk factors for HHcy included gender, age and hypertension. © 2015, Editorial Department of Fudan University Journal of Medical Sciences. All right reserved

He J.-F.,Pudong Hospital | Gu G.-M.,Pudong Hospital | Long D.-H.,Guangzhou University
Chinese Journal of Tissue Engineering Research | Year: 2014

Background: Nerve growth factor (NGF) belongs to a biological macromolecule that is difficult to pass through the blood-brain barrier. However, a retroviral vector carrying exogenous gene can be stably inserted and integrated into the host cell genome, which is suitable for gene therapy. Objective: To study the gene expression of recombinant retroviral vector carrying rat NGF gene in neural stem cells. Methods: The rat NGF gene was inserted into a retroviral vector pLEGFP-N1, which was transferred into packaging cells PT67 by Lipofectamine 2000. The positive clones in virus supernatant were collected by G418 selection and used to infect neural stem cells. After that, the NGF expression was tested by enzyme linked immunosorbent assay and the biological competence by PC12 cells, and then morphological change of neural stem cells and cell typing were examined by fluorescent microscope. Results and Conclusion: The neural stem cells could express extrinsic source NGF protein after the recombinant plasmid was infected into neural stem cells. The PC12 cells increased in the experimental group and stretched out long neurites. And the NGF protein could maintain the neural stem cell survival and stimulate their differentiation. These findings suggest that the neural stem cells carrying extrinsic source NGF gene could express NGF successfully, and the NGF protein induced the survival and differentiation of neural stem cells.

Yang X.,Shanghai JiaoTong University | Shen L.,Shanghai JiaoTong University | Lin Q.S.,Longyan First Hospital | Li R.,Pudong Hospital | And 2 more authors.
Journal of Craniofacial Surgery | Year: 2015

We report a case with both traumatic subdural effusion (TSE) and associated hydrocephalus. A collapse of the sinuses is known to be present in some infants with external hydrocephalus, but collapsed sinuses have not been previously described in patients with TSE and associated hydrocephalus. Therefore, a preoperative magnetic resonance imaging venography was performed, with thrombosis in the left transverse and sigmoid sinuses identified. The infant was treated with subdural peritoneostomy. We hypothesized that an occlusive cerebral venous sinus thrombosis may well be the culprit, or an exacerbating factor for TSE associated with hydrocephalus. © 2015 Mutaz B. Habal, MD.

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