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Mazzatenta A.,University of Chieti Pescara | Mazzatenta A.,CNR Institute of Neuroscience | Mazzatenta A.,University of Pisa | Pokorski M.,Public Higher Medical Professional School | And 6 more authors.
Respiratory Physiology and Neurobiology | Year: 2015

Alzheimer's disease (AD) is a profoundly life changing condition and once diagnosis occurs, this is typically at a relatively late stage into the disease process. Therefore, a shift to earlier diagnosis, which means several decades before the onset of the typical manifestation of the disease, will be an important step forward for the patient.A promising diagnostic and screening tool to answer this purpose is represented by breath and exhaled volatile organic compounds (VOCs) analysis. In fact, human exhaled breath contains several thousand of VOCs that vary in abundance and number in correlation with the physiological status. The exhaled VOCs reflect the metabolism, including the neuronal ones, in healthy and pathological conditions. A growing number of studies clearly demonstrate the effectiveness of VOCs analysis in identifying pathologies, including neurodegenerative diseases. In the present study we recorded, in real time, breath parameters and exhaled VOCs. We were able to demonstrate a significant alteration in breath parameters induced by the pathology of AD. Further, we provide the putative VOCs fingerprint of AD. These vital findings are an important step toward the early diagnosis of AD. © 2014 Elsevier B.V.


Kurpas D.,Wroclaw Medical University | Mroczek B.,Pomeranian Medical University | Knap-Czechowska H.,H. Knap Czechowska Outpatient Clinic | Bielska D.,Medical University of Bialystok | And 7 more authors.
Respiratory Physiology and Neurobiology | Year: 2013

The purpose of this study was to determine quality of life (QoL) and acceptance of illness in patients with chronic respiratory diseases. The study involved 315 adult patients of the mean age of 63.9 ± 15.7 years. The World Health Organization Quality of Life Instrument Short Form and the Acceptance of Illness Scale were used. The mean score for QoL was 2.0 ± 1.3. The highest scores were obtained in the Social Relationship Domain (13.9 ± 2.7) and the lowest in the Environmental Domain (10.5 ± 2.2). The strongest correlations within QoL domains were noted between Physical and Psychological Domains: r= 0.611 (p< 0.001), Psychological and Social Domains: r= 0.605 (p< 0.001). The overall degree of illness acceptance was low (26.0 ± 7.8). The strongest correlations were observed between illness acceptance and Physical: r= 0.591 (p< 0.001) and Psychological Domains: r= 0.450 (p< 0.001). We conclude that illness acceptance can be augmented by improving the patient's clinical state and by the provision of psychological support and QoL by improving the Psychological and Environmental Domains. © 2013 Elsevier B.V.


Kurpas D.,Wroclaw Medical University | Mroczek B.,Pomeranian Medical University | Jasinska M.,Pomeranian Medical University | Bielska D.,Medical University of Bialystok | And 9 more authors.
Advances in Experimental Medicine and Biology | Year: 2013

The purpose of this study was to analyze health behaviors of patients with chronic respiratory disease and the correlations between health behaviors (HB) and quality of life (QoL). The study involved 256 adult patients (135 women and 121 men), mean age was 62.6 ± 16.1 years, with chronic respiratory disease. The research tools consisted of the Health Behavior Inventory (HBI) and the World Health Organization Quality of Life Instrument Short Form. The mean general score for HB was 85.6 ± 17.8. Most patients (74 persons) obtained high scores (7-10 stens). The strongest correlations between QoL and HB were as follows: Psychological Domain correlated with positive mental attitude (r = 0.308, p < 0.001), healthy eating habits (r = 0.224, p = 0.001), and with the level of health behaviors (r = 0.222, p = 0.003); Social Relationship Domain correlated with positive mental attitude (r = 0.282, p < 0.001) and healthy eating habits (r = 0.238, p < 0.001). We conclude that QoL in patients with chronic respiratory diseases is significantly shaped by their health behaviors. © 2013 Springer Science+Business Media Dordrecht.


Dudek K.,Wroclaw University of Technology | Kedzia W.,Non public Higher Medical School in Wroclaw | Kedzia E.,Wroclaw Medical University | Kedzia A.,Non public Higher Medical School in Wroclaw | Derkowski W.,Public Higher Medical Professional School
Anatomical Science International | Year: 2016

The goal of this study was to present a procedure that would enable mathematical analysis of the increase of linear sizes of human anatomical structures, estimate mathematical model parameters and evaluate their adequacy. Section material consisted of 67 foetuses—rectus abdominis muscle and 75 foetuses- biceps femoris muscle. The following methods were incorporated to the study: preparation and anthropologic methods, image digital acquisition, Image J computer system measurements and statistical analysis method. We used an anthropologic method based on age determination with the use of crown-rump length—CRL (V–TUB) by Scammon and Calkins. The choice of mathematical function should be based on a real course of the curve presenting growth of anatomical structure linear size Ύ in subsequent weeks t of pregnancy. Size changes can be described with a segmental-linear model or one-function model with accuracy adequate enough for clinical purposes. The interdependence of size–age is described with many functions. However, the following functions are most often considered: linear, polynomial, spline, logarithmic, power, exponential, power-exponential, log-logistic I and II, Gompertz’s I and II and von Bertalanffy’s function. With the use of the procedures described above, mathematical models parameters were assessed for V-PL (the total length of body) and CRL body length increases, rectus abdominis total length h, its segments hI, hII, hIII, hIV, as well as biceps femoris length and width of long head (LHL and LHW) and of short head (SHL and SHW). The best adjustments to measurement results were observed in the exponential and Gompertz’s models. © 2016 The Author(s)


Andrzejewski W.,University School of Physical Education in Wroclaw | Kassolik K.,University School of Physical Education in Wroclaw | Kobierzycki C.,University School of Physical Education in Wroclaw | Kobierzycki C.,Wroclaw Medical University | And 8 more authors.
Folia Histochemica et Cytobiologica | Year: 2015

Introduction. Numerous investigations have been carried out to describe the role of massage in preparing for and restoring efficiency after physical exercise. Furthermore, vascular endothelial growth factor (VEGF) enhances blood vessel growth, and in effect contributes to the regeneration of tissues. Since its expression in active skeletal muscles has not been yet determined, the aim of this study was to investigate whether muscle massage performed before and during running exercise affects the expression of VEGF-A in muscles. Material and methods. The study was carried out on 75 adult Buffalo rats subjected to running exercise training for 10 weeks. Rats were massaged prior (group PM) or during exercise (group M) or were not massaged (group C). The massage consisted of spiral movements along the plantar surface of flexor digitorum brevis muscle. After 1, 3, 5, 7 and 10 week of training, five rats from every group were anesthetized and immunohistochemistry, Western blot, and PCR analyses were performed on obtained muscle tissue to determine VEGF-A expression. Results. After the first week of training, a significant increase of VEGF-A gene expression analyzed by qPCR in muscle tissue was observed in the PM group, whereas in the third week, the predominant growth of studied marker was seen in the M group. Increased VEGF-A expression on the protein level was observed in both massaged groups following the first week. A moderate positive correlation was found between the expression of the VEGF-A gene and protein in all experimental groups (r = 0.389). Conclusion. Short-term repeated massage may contribute to processes of creation of new and development of already existing vascular networks in the skeletal muscle tissue during increased exercise. © Polish Society for Histochemistry and Cytochemistry.


Andrzejewski W.,University School of Physical Education in Wroclaw | Kassolik K.,University School of Physical Education in Wroclaw | Dziegiel P.,University School of Physical Education in Wroclaw | Dziegiel P.,Wroclaw Medical University | And 7 more authors.
BioMed Research International | Year: 2014

Physical exercise and massage are regarded as key factors in regulating tendon structure. However, information on the mechanism through which massage influences the structure and biology of a tendon is scarce. In this study, we attempted to define the impact of these two activities on rat tendons by using morphological and molecular techniques, determining the expression of VEGF-A, FGF-2, and CD34 in the tendons of rats subjected to 10 weeks of physical exercise (running) with massage of varied duration. The group of rats that was trained and massaged during the entire study was characterized by the highest expression of these markers, compared to the rats subjected to massage before training and to the control group subjected to physical exercises only. The greatest significant differences, compared to the control, were noted in the expression of all the studied markers at mRNA level, and in the case of VEGF-A, at protein level, in the third and fifth weeks of the experiment. The results of this study could point to the synergistic impact of simultaneous massage and physical exercise on the expression of angiogenesis markers in rat tendons. © 2014 Waldemar Andrzejewski et al.


Wisniewski A.,Polish Academy of Sciences | Kowal A.,Wrocław University | Wyrodek E.,Wroclaw Medical University | Nowak I.,Polish Academy of Sciences | And 8 more authors.
Tissue Antigens | Year: 2015

Human leukocyte antigen-G (HLA-G) is a nonclassical HLA class I molecule absent from most normal tissues but detected in many malignant tumors. It is recognized by cells of the immune system using LILRB1, KIR2DL4 and LILRB2 receptors. We attempted to find out whether some polymorphisms of HLA-G, LILRB1 and KIR2DL4 genes are associated with susceptibility to nonsmall cell lung cancer (NSCLC). Four polymorphisms in HLA-G, i.e. -964A>G (rs1632947), -725C>G>T (rs1233334), -716T>G (rs2249863) in the promoter, and a 14 base pair insertion/deletion (14bp indel) in the 3′-untranslated region (3′UTR), and five in LILRB1 - 5651G>A (rs41308748) in intron 14, 5717C>T L622L (rs1061684), 5724G>A E625K (rs16985478), 5774 C>A P641P (rs41548213) in exon 15, and 5806C>T (rs8101240) in 3′UTR - as well as 9620 9A/10A (rs11410751) polymorphism in exon 7 of KIR2DL4 were typed using different laboratory techniques. Only one single nucleotide polymorphism (SNP) in HLA-G (-964A>G) and one in LILRB1 (5724G>A) were found to influence the risk of NSCLC. In addition, 5724G>A was associated with protection from tumor cell infiltration of regional lymph nodes. Most importantly, we detected HLA-G and LILRB1 expression in tumor specimens, but no correlation with genetic polymorphisms was observed. HLA-G and LILRB1 protein expression levels in tumor tissue were significantly correlated with tumor stage. © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.


Andrzejewski W.,University School of Physical Education in Wroclaw | Kassolik K.,University School of Physical Education in Wroclaw | Dziegiel P.,University School of Physical Education in Wroclaw | Dziegiel P.,Wroclaw Medical University | And 9 more authors.
In Vivo | Year: 2015

Background: Physical exercise is now a widely known and studied factor of the proper functioning of living organisms. Many questions remain unanswered concerning various aspects of the changes in the morphology of structures subjected to chronic physical exercise. Materials and Methods: This study was conducted on fifty Buffalo strain rats, randomly divided into two equal (experimental and control) groups. All animals were subjected to physical training on a running track for 10 weeks, whereas only in the experimental group, massage was additionally applied five-times per week. Results. An increase in the percentage of collagen fibers in tendons with the smallest diameter (≤100 nm) was observed only in the experimental group in week 3, followed by a decrease in weeks 5 and 7. A subsequent repeated increase was observed in week 10 of the study. No significant differences were observed for either study group in the number of collagen fibers based on fiber diameter (101-200 nm, 201-300 nm and 301-400 nm). Conclusion: The results of this preliminary study showed that long-term massage performed during running training may initiate for small structural changes in the rat tendon. Further morphological studies with prolonged observation periods are recommended. © 2015, International Institute of Anticancer Research. All rights reserved.

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