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Ostrowiec Świętokrzyski, Poland

Dudek K.,Wroclaw University of Technology | Kedzia W.,Non public Higher Medical School in Wroclaw | Kedzia E.,Wroclaw Medical University | Kedzia A.,Non public Higher Medical School in Wroclaw | Derkowski W.,Public Higher Medical Professional School
Anatomical Science International

The goal of this study was to present a procedure that would enable mathematical analysis of the increase of linear sizes of human anatomical structures, estimate mathematical model parameters and evaluate their adequacy. Section material consisted of 67 foetuses—rectus abdominis muscle and 75 foetuses- biceps femoris muscle. The following methods were incorporated to the study: preparation and anthropologic methods, image digital acquisition, Image J computer system measurements and statistical analysis method. We used an anthropologic method based on age determination with the use of crown-rump length—CRL (V–TUB) by Scammon and Calkins. The choice of mathematical function should be based on a real course of the curve presenting growth of anatomical structure linear size Ύ in subsequent weeks t of pregnancy. Size changes can be described with a segmental-linear model or one-function model with accuracy adequate enough for clinical purposes. The interdependence of size–age is described with many functions. However, the following functions are most often considered: linear, polynomial, spline, logarithmic, power, exponential, power-exponential, log-logistic I and II, Gompertz’s I and II and von Bertalanffy’s function. With the use of the procedures described above, mathematical models parameters were assessed for V-PL (the total length of body) and CRL body length increases, rectus abdominis total length h, its segments hI, hII, hIII, hIV, as well as biceps femoris length and width of long head (LHL and LHW) and of short head (SHL and SHW). The best adjustments to measurement results were observed in the exponential and Gompertz’s models. © 2016 The Author(s) Source

Wisniewski A.,Polish Academy of Sciences | Kowal A.,Wroclaw University | Wyrodek E.,Wroclaw Medical University | Nowak I.,Polish Academy of Sciences | And 8 more authors.
Tissue Antigens

Human leukocyte antigen-G (HLA-G) is a nonclassical HLA class I molecule absent from most normal tissues but detected in many malignant tumors. It is recognized by cells of the immune system using LILRB1, KIR2DL4 and LILRB2 receptors. We attempted to find out whether some polymorphisms of HLA-G, LILRB1 and KIR2DL4 genes are associated with susceptibility to nonsmall cell lung cancer (NSCLC). Four polymorphisms in HLA-G, i.e. -964A>G (rs1632947), -725C>G>T (rs1233334), -716T>G (rs2249863) in the promoter, and a 14 base pair insertion/deletion (14bp indel) in the 3′-untranslated region (3′UTR), and five in LILRB1 - 5651G>A (rs41308748) in intron 14, 5717C>T L622L (rs1061684), 5724G>A E625K (rs16985478), 5774 C>A P641P (rs41548213) in exon 15, and 5806C>T (rs8101240) in 3′UTR - as well as 9620 9A/10A (rs11410751) polymorphism in exon 7 of KIR2DL4 were typed using different laboratory techniques. Only one single nucleotide polymorphism (SNP) in HLA-G (-964A>G) and one in LILRB1 (5724G>A) were found to influence the risk of NSCLC. In addition, 5724G>A was associated with protection from tumor cell infiltration of regional lymph nodes. Most importantly, we detected HLA-G and LILRB1 expression in tumor specimens, but no correlation with genetic polymorphisms was observed. HLA-G and LILRB1 protein expression levels in tumor tissue were significantly correlated with tumor stage. © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. Source

Andrzejewski W.,University School of Physical Education in Wroclaw | Kassolik K.,University School of Physical Education in Wroclaw | Kobierzycki C.,University School of Physical Education in Wroclaw | Kobierzycki C.,Wroclaw Medical University | And 8 more authors.
Folia Histochemica et Cytobiologica

Introduction. Numerous investigations have been carried out to describe the role of massage in preparing for and restoring efficiency after physical exercise. Furthermore, vascular endothelial growth factor (VEGF) enhances blood vessel growth, and in effect contributes to the regeneration of tissues. Since its expression in active skeletal muscles has not been yet determined, the aim of this study was to investigate whether muscle massage performed before and during running exercise affects the expression of VEGF-A in muscles. Material and methods. The study was carried out on 75 adult Buffalo rats subjected to running exercise training for 10 weeks. Rats were massaged prior (group PM) or during exercise (group M) or were not massaged (group C). The massage consisted of spiral movements along the plantar surface of flexor digitorum brevis muscle. After 1, 3, 5, 7 and 10 week of training, five rats from every group were anesthetized and immunohistochemistry, Western blot, and PCR analyses were performed on obtained muscle tissue to determine VEGF-A expression. Results. After the first week of training, a significant increase of VEGF-A gene expression analyzed by qPCR in muscle tissue was observed in the PM group, whereas in the third week, the predominant growth of studied marker was seen in the M group. Increased VEGF-A expression on the protein level was observed in both massaged groups following the first week. A moderate positive correlation was found between the expression of the VEGF-A gene and protein in all experimental groups (r = 0.389). Conclusion. Short-term repeated massage may contribute to processes of creation of new and development of already existing vascular networks in the skeletal muscle tissue during increased exercise. © Polish Society for Histochemistry and Cytochemistry. Source

Mazzatenta A.,University of Chieti Pescara | Mazzatenta A.,CNR Institute of Neuroscience | Mazzatenta A.,University of Pisa | Pokorski M.,Public Higher Medical Professional School | And 6 more authors.
Respiratory Physiology and Neurobiology

Alzheimer's disease (AD) is a profoundly life changing condition and once diagnosis occurs, this is typically at a relatively late stage into the disease process. Therefore, a shift to earlier diagnosis, which means several decades before the onset of the typical manifestation of the disease, will be an important step forward for the patient.A promising diagnostic and screening tool to answer this purpose is represented by breath and exhaled volatile organic compounds (VOCs) analysis. In fact, human exhaled breath contains several thousand of VOCs that vary in abundance and number in correlation with the physiological status. The exhaled VOCs reflect the metabolism, including the neuronal ones, in healthy and pathological conditions. A growing number of studies clearly demonstrate the effectiveness of VOCs analysis in identifying pathologies, including neurodegenerative diseases. In the present study we recorded, in real time, breath parameters and exhaled VOCs. We were able to demonstrate a significant alteration in breath parameters induced by the pathology of AD. Further, we provide the putative VOCs fingerprint of AD. These vital findings are an important step toward the early diagnosis of AD. © 2014 Elsevier B.V. Source

Kurpas D.,Wroclaw Medical University | Mroczek B.,Pomeranian Medical University | Knap-Czechowska H.,H. Knap Czechowska Outpatient Clinic | Bielska D.,Medical University of Bialystok | And 7 more authors.
Respiratory Physiology and Neurobiology

The purpose of this study was to determine quality of life (QoL) and acceptance of illness in patients with chronic respiratory diseases. The study involved 315 adult patients of the mean age of 63.9 ± 15.7 years. The World Health Organization Quality of Life Instrument Short Form and the Acceptance of Illness Scale were used. The mean score for QoL was 2.0 ± 1.3. The highest scores were obtained in the Social Relationship Domain (13.9 ± 2.7) and the lowest in the Environmental Domain (10.5 ± 2.2). The strongest correlations within QoL domains were noted between Physical and Psychological Domains: r= 0.611 (p< 0.001), Psychological and Social Domains: r= 0.605 (p< 0.001). The overall degree of illness acceptance was low (26.0 ± 7.8). The strongest correlations were observed between illness acceptance and Physical: r= 0.591 (p< 0.001) and Psychological Domains: r= 0.450 (p< 0.001). We conclude that illness acceptance can be augmented by improving the patient's clinical state and by the provision of psychological support and QoL by improving the Psychological and Environmental Domains. © 2013 Elsevier B.V. Source

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