Spinato J.,McGill University |
Spinato J.,Public Health Ontario Laboratory |
Boivin E.,Laboratoire Of Sante Publique Du Quebec |
Belanger-Trudelle E.,Laboratoire Of Sante Publique Du Quebec |
And 3 more authors.
BMC Microbiology | Year: 2016
Background: The increasing emergence of drug-resistant tuberculosis presents a threat to the effective control of tuberculosis (TB). Rapid detection of drug-resistance is more important than ever to address this scourge. The purpose of this study was to genotypically characterize the first-line antitubercular drug-resistant isolates collected over 11 years in Quebec. Results: The main mutations found in our resistant strains collection (n = 225) include: the S315T substitution in katG (50.2 %), the -15 C/T mutation in the inhA promoter (29 %); the S531L substitution in rpoB (43 %); the deletion 8 bp 446 / + R140S in pncA (72.9 %); the M306I (35.7 %) and M306V (21.4 %) substitutions in embB. Ten of the mutations in katG and 4 mutations identified in pncA were previously undescribed. Conclusion: Screening of mutations conferring resistance to first-line antituberculous drugs using DNA-sequencing approach seems to be feasible and would drastically shorten the time to determine the resistance profile compared to the proportion method. © 2016 The Author(s).
Tsang R.S.W.,National Microbiology Laboratory |
Lefebvre B.,Institute National Of Sante Publique Du Quebec |
Jamieson F.B.,Public Health Ontario Laboratory |
Jamieson F.B.,University of Toronto |
And 4 more authors.
Canadian Journal of Microbiology | Year: 2012
This study examined serogroup B meningococci (MenB) from invasive meningococcal disease (IMD) cases in the provinces of Québec and Ontario in the last decade by multilocus sequence typing (MLST) to determine their sequence types (STs) and clonal complexes (CCs). Forty isolates from individual MenB IMD cases were found to belong to 8 related STs, with ST-336 being the founding ST and the other 7 STs being single locus variants of ST-336. Eleven isolates belonged to ST-336, 23 belonged to ST-5571, and the other 6 were represented individually by a single different ST. All but 1 of these 40 isolates have the PorA variable-region type of P1.22,14,36. Interrogation of the Neisseria MLST web site with the present finding did not put any of the 8 related STs into known CCs. Since these 8 related STs were common causes of IMD, with ST-5571 being the most frequently identified ST in Ontario and ST-336 the third most common ST identified in Québec, we propose that ST-336 and its related STs is a potentially new meningococcal clonal complex that is endemic in the Canadian provinces of Québec and Ontario, and they constitute a common cause of IMD.
Peci A.,Public Health Ontario Laboratory |
Marchand-Austin A.,Public Health Agency of Canada |
Winter A.-J.,Public Health Ontario Laboratory |
Gubbay J.B.,Public Health Ontario Laboratory |
Gubbay J.B.,University of Toronto
Epidemiology and Infection | Year: 2013
The objective of this study was to determine the optimal number of respiratory samples per outbreak to be tested for institutional respiratory outbreaks in Ontario. We reviewed respiratory samples tested for respiratory viruses by multiplex PCR as part of outbreak investigations. We documented outbreaks that were positive for any respiratory viruses and for influenza alone. At least one virus was detected in 1454 (85·2%) outbreaks. The ability to detect influenza or any respiratory virus increased as the number of samples tested increased. When analysed by chronological order of when samples were received at the laboratory, percent positivity of outbreaks testing positive for any respiratory virus including influenza increased with the number of samples tested up to the ninth sample, with minimal benefit beyond the fourth sample tested. Testing up to four respiratory samples per outbreak was sufficient to detect viral organisms and resulted in significant savings for outbreak investigations. © Cambridge University Press 2012.
Tsang R.S.W.,Public Health Agency of Canada |
Hoang L.,BC Public Health Microbiology and Reference Laboratory |
Tyrrell G.,Provincial Laboratory for Public Health |
Horsman G.,Saskatchewan Disease Control Laboratory |
And 5 more authors.
Journal of Medical Microbiology | Year: 2015
We previously reported a shift in the electrophoretic type (ET) of invasive MenC in Canada from predominantly ET-15 to ET-37 in the post-MenC conjugate vaccine period. This study sought to confirm this trend by examining all culture-confirmed invasive MenC case isolates in Canada in the period from 1 January 2009 to 31 December 2013. Of the 50 MenC isolates, 18 belonged to ET-15, 28 belonged to ET-37 (but not ET-15), and four belonged to other clonal types. Analysis of the serotype and serosubtype antigens, porA and fetA gene sequences provided data to show that invasive MenC belonging to ET-15 and ET-37 were two very different subpopulations within the ST-11 clonal complex. Sequence analysis of the fHbp genes suggested that 12 different types of factor H-binding protein were found among the ET-15 isolates while 86% of ET-37 isolates were found to have fHbp genes predicted to encode peptide 22. The nadA gene in 12 MenC isolates was disrupted due to IS1301 insertion and 11 of these 12 isolates belonged to ET-15. Ten per cent of the invasive MenC were found to have a frame-shift mutation in their fHbp genes that predicted no fHbp produced. Significant diversity and frame-shift mutations of fHbp genes were found in invasive MenC strains in Canada. © 2015 The Authors.
Mallegol J.,Public Health Ontario Laboratory |
Fernandes P.,Cempra |
Melano R.G.,Public Health Ontario Laboratory |
Melano R.G.,University of Toronto |
Guyarda C.,University of Toronto
Antimicrobial Agents and Chemotherapy | Year: 2014
The activity of solithromycin was evaluated against clinical Legionella pneumophila serogroup 1 (Lp1) isolates (n=196) collected in Ontario, Canada, from 1980 to 2011. Its in vitro activity was compared to that of azithromycin (AZM) using the broth microdilution method. Solithromycin had a MIC50 of<0.015 μg/ml and a MIC90 of 0.031 μg/ml, making its activity at least 8-fold to 32-fold higher than that of AZM (MIC50 and MIC90, 0.125 μg/ml and 1 μg/ml, respectively). Ninety-nine percent of the isolates had MICs for solithromycin ranging from<0.015 μg/ml to 0.031 μg/ml, whereas 83.6% of the isolates showed MICs for AZM ranging from 0.062 μg/ml to 0.25 μg/ml. Interestingly, 96.7% (30 out of 31 clinical isolates) identified with higher AZM MICs (0.5 μg/ml to 2 μg/ml) belonged to the clinically prevalent sequence type 1. To investigate the intracellular activity of solithromycin, in vitro invasion assays were also performed against a subset of representative Lp1 isolates internalized within human lung epithelial cells. Solithromycin and AZM both inhibited growth of all intracellular Lp1 isolates at 1×or 8×MICs, displaying bacteriostatic effects, as would be expected with protein synthesis inhibitor rather than bactericidal activity. Solithromycin demonstrated the highest in vitro and intracellular potency against all Lp1 isolates compared to AZM. Given the rapid spread of resistance mechanisms among respiratory pathogens and the reported treatment failures in legionellosis, the development of this new fluoroketolide, already in phase 3 oral clinical studies, constitutes a promising alternative option for the treatment of legionellosis. Copyright © 2014, American Society for Microbiology. All Rights Reserved.