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Rakic A.,Public Health Institute of Split Dalmatian County | Stambuk-Giljanovic N.,University of Split
Environmental Monitoring and Assessment | Year: 2016

The purpose of this study was to evaluate the prevalence of Legionella spp. and compare the quality of hot water between four facilities for accommodation located in Southern Croatia (the Split-Dalmatian County). The research included data collection on the technical and technological characteristics in the period from 2009 to 2012. The survey included a type of construction material for the distribution and internal networks, heating system water heater type, and water consumption. Changes in water quality were monitored by determination of the physical and chemical parameters (temperature, pH, free chlorine residual concentrations, iron, zinc, copper and manganese) in the samples, as well as the presence and concentration of bacteria Legionella spp. The temperature is an important factor for the development of biofilms, and it is in negative correlation with the appearance of Legionella spp. Positive correlations between the Fe and Zn concentrations and Legionella spp. were established, while the inhibitory effect of a higher Cu concentration on the Legionella spp. concentration was proven. Legionella spp. were identified in 38/126 (30.2 %) of the water samples from the heating system with zinc-coated pipes, as well as in 78/299 (26.1 %) of the samples from systems with plastic pipes. A similar number of Legionella spp. positive samples were established regardless of the type of the water heating system (central or independent). The study confirms the necessity of regular microbial contamination monitoring of the drinking water distribution systems (DWDSs). © 2016, Springer International Publishing Switzerland. Source

Knezovic Z.,Public Health Institute of Split Dalmatian County | Trgo M.,University of Split | Sutlovic D.,University of Split
Process Safety and Environmental Protection | Year: 2016

In this study, bioaccumulation of mercury in humans was examined by performing analysis on 165 meconium samples of newborn children in Split and Dalmatian County. Total mercury determination was performed on the Advanced Mercury Analyser AMA 254 without previous sample preparation. Mercury was identified from all examined samples, where mean value of concentration for the whole studied group was 58.19ngg-1, median 35.69ngg-1 and concentration range from 3.04 to 394.69ngg-1, respectively. The analysis of the results showed correlation of mercury concentration in meconium with sociodemographic characteristics of the studied group such us living residence place, dietary habits and influence of amalgam fillings. The highest concentrations of mercury were observed in the group in which mothers consumed fish, particularly in the coastal living area. Slightly higher concentrations of mercury in samples were found in the urban living area compared to the rural residence. This was probably due to the strong effect of disposed mercury from the industrial plants into the environment of Split and Dalmatian County during last 50 years. The relation of sea food consumption and mercury content in meconium samples, confirms that food path is responsible for its bioaccumulation and biomagnification from the environment into the living organisms, particularly in humans. © 2016 The Institution of Chemical Engineers. Source

Sutlovic D.,University of Split | Boric I.,General Hospital Dubrovnik | Sliskovic L.,University of Split | Popovic M.,University of Split | And 4 more authors.
Legal Medicine | Year: 2016

Dual-energy X-ray absorptiometry (DXA) scanning is a gold standard for bone mineral density measurement and diagnosis of primary and secondary osteoporosis in living persons. DXA is becoming widespread when analysing archaeological material, and is considered to provide an accurate diagnosis of osteoporosis in skeletal samples.The aim of this study was to explain the differences in results between bone mineral density (obtained with DXA) and chemical determination of calcium and phosphorus concentrations in skeletal remains. We examined bone mineral density (BMD) and mineral content of femoral bone samples exhumed from mass graves of the Second World War. BMD was determined by Hologic QDR 4500 C (S/N 48034) Bone Densitometer. Concentrations of calcium and phosphorus were determined with AAS (Atomic absorption spectroscopy) and UV/VIS (Ultraviolet-visible) spectroscopy.The results obtained in this study do not support the hypothesis according to which BMD measured by DXA scan has positive correlation with chemically determined concentrations of calcium and phosphorus in bones, especially in acidic soils where there was significant impact of diagenesis observed. © 2016 Elsevier Ireland Ltd. Source

Glucina D.,University of Split | Britvic D.,University of Split | Lasic D.,University of Split | Dedic M.,University of Split | And 4 more authors.
Psychiatria Danubina | Year: 2010

Diagnostic Interview for Genetic Studies (DIGS) is a modern structured interview schedule that has been in use since 1994. Main purpose of the DIGS is to record information regarding to a subject's functioning and psychopathology and it was specifically designed for psychiatric genetic studies. The DIGS is also suitable for making diagnosis, evaluation of comorbidity and other researches. It contents items and sections and has a semi-structured design that gives interviewers the freedom needed to extract the best information possible. The validity of a Croatian version of the DIGS was investigated. The original English version was initially translated into Croatian. The Croatian version was then back-translated and compared with the original. In this paper we will describe each item that DIGS contents as well as the use of this diagnostic instrument. © Medicinska naklada. Source

Sutlovic D.,University of Split | Stipisic A.,Public Health Institute of Split Dalmatian County | Marusic J.,Public Health Institute of Split Dalmatian County | Pavlov J.,Public Health Institute of Split Dalmatian County | And 4 more authors.
Croatica Chemica Acta | Year: 2010

Elemental status of mediaeval individuals from Naklice burial site (Southern Croatia) was analyzed and their diet was reconstructed. Samples from different human bones were taken from 16 individuals who died in the 9 th century and were recently excavated from Naklice burial site. The metal content of iron, lead, cadmium, magnum, zinc, copper, strontium, and calcium were determined by atomic absorption spectrometry (AAS) in flame mode. Mercury concentration was determined by three direct consecutive measures taken with a mercury analyzer. When comparing our results to the modern bone heavy metal concentrations, it is obvious that Cu, Fe, Ca and Sr greatly exceeded the values while concentration of Zn and Pb were lower. Concentration of Sr was about ten times higher than in modern bone samples. Due to environmental contamination, lead concentration in studied bones was lower than in modern bone samples. According to our results we concluded that the probable main dietary components of individuals excavated from Naklice burial site from Early Mediaeval period were leafy vegetables, legumes and small amounts of cereals. Source

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