Public Health Institute Nis

Serbia

Public Health Institute Nis

Serbia
SEARCH FILTERS
Time filter
Source Type

PubMed | University of Rome La Sapienza, University of Niš and Public Health Institute Nis
Type: Journal Article | Journal: Foodborne pathogens and disease | Year: 2016

We report data on the Toxocara seroprevalence evidenced in 2015 from samples of 40 children and 298 adults of the population living in different areas of Serbia, and on possible association of certain variables with infection. Detection of specific antibodies was performed using an enzyme-linked immunosorbent assay; all ambiguous results and part of the positive and negative sera were further analyzed by confirmatory Western blot test. An overall 23.5% seroprevalence was noticed, which was confirmed in 13.0% of the examined population with no significant difference regarding the age (children=10.0%; adults=13.4%) or by country area (East=18.2%; North=15.5%, Southeastern=9.5%; p=0.005). In contrast, the group of adult women proved more reactive than men (p=0.001), and subjects both who spend spare time in square/parks (p=0.041) and with positive onychophagy (p=0.001) habit turned out more exposed to the infection. Possible reasons of these differences were analyzed, and the medical, veterinary, and economic impact of this soil-transmitted zoonosis were discussed.


PubMed | University of Belgrade, University of Niš, King's College and Public Health Institute Nis
Type: Journal Article | Journal: Mycoses | Year: 2016

Despite the increasing of onychomycosis caused by Candida spp., in referent literature, there is still data insufficiency about this nail infection. The objectives of this retrospective study were to determine epidemiological characteristics of Candida onychomycosis, the antifungal susceptibility of isolated species in vitro, and to compare the results of antifungal susceptibility testing with conducted treatment in period from 2011 to the end of March 2015. Out of 761 patients who were underwent clinical and mycological examinations, 137 had Candida species isolated from nails. The dominant species was Candida albicans (C. albicans) (36.59%) followed by C. parapsilosis (23.78%), C. krusei (9.76%), and C. guilliermondii (6.71%). Antifungal susceptibility in vitro testing showed good susceptibility to antimycotics, except C. krusei, which was resistance to fluconazole (FCZ) and isolates of C. tropicalis and C. glabrata which were dose dependent to itraconazole (ITZ) and fluconazole. Evaluation of medical histories determined that combined therapy, which included pulsed systemic regimen of ITZ with topical application of clotrimazole, had better clinical outcomes regarding the proscribed only topical application of clotrimazole. Multidisciplinary approach of dermatologists and mycologists is required in solving the problem of onychomycosis, which is the dominant nail disease.


Gabrielli S.,University of Rome La Sapienza | Otasevic S.,University of Niš | Otasevic S.,Public Health Institute Nis | Ignjatovic A.,Public Health Institute Nis | And 7 more authors.
Vector-Borne and Zoonotic Diseases | Year: 2015

During the years 2012-2014, a total of 158 outdoor dogs from Pančevo and Crossed D signurcrossed d signevo (northern Serbia) and Niš and Prokuplje (southern Serbia) were submitted to molecular analyses (PCR and sequencing) for canine babesioses. An overall prevalence of 21.5% was found, due to the species Babesia sp. 'spanish dog' (10.1%), B. gibsoni (5.7%), B. canis vogeli (1.9%), B. caballi (1.9%), and B. microti (1.9%). In addition, sequence analysis showed the presence of Hepatozoon canis in a dog from Niš. No significant difference between infected and noninfected dogs was found by age, sex, and place of residence, whereas there was difference regarding the presence of ticks (p<0.005) and application of preventive measures such as applying of antitick drugs/devices. Moreover, a significant difference was established by area: Dogs from Prokuplje showed infection rates (59.1%) higher than dogs from Pančevo (11.9%), Niš (4.5), and Crossed D signurcrossed d signevo (where infected dogs were not found), and a different geographical distribution of the species was found. The presence of so many Babesia species and the first identification of H. canis will allow investigations on the pathogenic role played by each one and suggests entomological studies on the tick species that are more suitable vectors for each of them. Finally, the presence of so many infected dogs offers the opportunity of evaluating the hypothesis of a possible zoonotic role of babesial species affecting dogs. © Copyright 2015, Mary Ann Liebert, Inc. 2015.


Tasic-Otasevic S.,University of Niš | Tasic-Otasevic S.,Public Health Institute Nis | Gabrielli S.,University of Rome La Sapienza | Trenkic-Bozinovic M.,Clinical Centar of Nis | And 6 more authors.
Comparative Immunology, Microbiology and Infectious Diseases | Year: 2016

Thelazia callipaeda eyeworm has been frequently reported parasitizing humans in Asia. In Europe, the parasite is endemic in wild and domestic carnivores and only eight cases have been reported in humans so far. We describe the first case of human thelaziosis in Serbia, along with two cases in dogs from the same area. A One Health approach, based on cooperation amongst veterinarians and physicians, is strongly advised for this emerging infection in order to assess the risk for and prevent of the zoonotic infection. © 2016 Elsevier Ltd.


PubMed | University of Rome La Sapienza, University of Belgrade, University of Niš, 5 Scientific Veterinary Institute Novi Sad and Public Health Institute Nis
Type: Journal Article | Journal: Vector borne and zoonotic diseases (Larchmont, N.Y.) | Year: 2015

During the years 2012-2014, a total of 158 outdoor dogs from Panevo and urevo (northern Serbia) and Ni and Prokuplje (southern Serbia) were submitted to molecular analyses (PCR and sequencing) for canine babesioses. An overall prevalence of 21.5% was found, due to the species Babesia sp. spanish dog (10.1%), B. gibsoni (5.7%), B. canis vogeli (1.9%), B. caballi (1.9%), and B. microti (1.9%). In addition, sequence analysis showed the presence of Hepatozoon canis in a dog from Ni. No significant difference between infected and noninfected dogs was found by age, sex, and place of residence, whereas there was difference regarding the presence of ticks (p<0.005) and application of preventive measures such as applying of antitick drugs/devices. Moreover, a significant difference was established by area: Dogs from Prokuplje showed infection rates (59.1%) higher than dogs from Panevo (11.9%), Ni (4.5), and urevo (where infected dogs were not found), and a different geographical distribution of the species was found. The presence of so many Babesia species and the first identification of H. canis will allow investigations on the pathogenic role played by each one and suggests entomological studies on the tick species that are more suitable vectors for each of them. Finally, the presence of so many infected dogs offers the opportunity of evaluating the hypothesis of a possible zoonotic role of babesial species affecting dogs.


PubMed | University of Belgrade, University of Niš and Public Health Institute Nis
Type: | Journal: Tissue & cell | Year: 2016

The aim of this research was to quantify the changes in the morphology and density of the anterior pituitary folliculostellate (FS) and luteinizing hormone (LH) cells. Material was tissue of the pituitary gland of the 14 male cadavers. Tissue slices were immunohistochemically stained with monoclonal anti-LH antibody and polyclonal anti-S100 antibody for the detection of LH and FS cells, respectively. Digital images of the stained slices were afterwards morphometrically analyzed by ImageJ. Results of the morphometric analysis showed significant increase of the FS cells volume density in cases older than 70 years. Volume density of the LH cells did not significantly change, whereas their area significantly increased with age. Nucleocytoplasmic ratio of the LH cells gradually decreased and became significant after the age of 70. Finally, volume density of the FS cell significantly correlated with LH cells area and nucleocytoplasmic ratio. From all above cited, we concluded that in men, density and size of the FS cells increase with age. Long-term hypertrophy of the LH cells results in their functional decline after the age of 70. Strong correlation between FS cells and LH cells morphometric parameters might point to age-related interaction between these two cell groups.


PubMed | University of Niš, State University of Novi Pazar, Academy for Criminalistic and Police Studies Belgrade and Public Health Institute Nis
Type: Journal Article | Journal: Central European journal of public health | Year: 2013

Mortality has been shown to increase with heat waves. Serbia experienced the hottest heat wave in July 2007. In this study, we examined patterns of non-traumatic excess mortality in Belgrade during this event.The numbers of deaths observed during the 9-day heat wave were compared to those expected on the basis of mortality rates reported for the previous eight years and two following years. Excess mortality was analyzed by age, gender and cause of death.There was a total of 167 excess deaths (38%) between 16 and 24 July. People aged 75 years and older accounted for 151 (90%) of all excess deaths. An increase of mortality among elderly was 76% in comparison to the baseline mortality. Excess female mortality was over two times higher than excess male mortality (54% : 23%). The biggest increase in mortality was from diabetes mellitus (286%), chronic kidney disease (200%), respiratory system diseases (73%), and nervous system diseases (67%). Cardiovascular and malignant neoplasms mortality accounted for the highest absolute numbers of excess deaths (77 and 49, respectively). There was no decrease in mortality in the 60-day period after the heat wave.There are several causes of an increase in heat-related mortality. The most vulnerable population group is the elderly females.


PubMed | University of Niš and Public Health Institute Nis
Type: | Journal: Pediatrics international : official journal of the Japan Pediatric Society | Year: 2016

Streptococcus pyogenes (S. pyogenes - group A Streptococcus [GAS]) is the etiological agent causing perineal infections in children: perianal infections, vulvovaginitis and balanitis. If it is not properly diagnosed and treated, it can persist for many months and can cause severe complications. Furthermore, the treatment with penicillin can be followed by failures and recurrences.We report here the prevalence of S. pyogenes isolates from the genital tract specimens taken from girls (1692) with symptoms of vulvovaginitis and boys (52) with balanitis in the municipality of Nis, Southeast-Serbia (the Western Balkans) in a ten years period, and the analysis of isolates by seasonal distribution, patient age and sensitivity to bacitracin and antimicrobial drugs used in the treatment of streptococcal infections.Streptococcal vulvovaginitis was diagnosed in 2.30% of examinees. From April to September, 64.10% cases of streptococcal vulvovaginitis were detected, and it was most common (71.79%) in girls aged 3 to 7 years. Streptococcal balanitis was diagnosed in two instances: in a four-year and a seven-year old boy. The S. pyogenes strains resistant to bacitracin were identified in the samples of five girls. Two isolates with M phenotype and five isolates with cMLSStreptococcal vulvovaginitis was diagnosed less often in our study, but it was still far more common than streptococcal balanitis in childhood. Bacitracin resistance of S. pyogenes strains should be taken into account in routine microbiological identification, and the detection of S. pyogenes isolates resistant to erythromycin requires the surveillance of their prevalence on our geographical territory. This article is protected by copyright. All rights reserved.


Lazarevic K.,Public Health Institute Nis
Central European journal of public health | Year: 2012

Contents of lead and arsenic were determined in 617 tobacco samples and 80 samples of cigarettes. The mean content of lead in tobacco was 0.93 microg/g (range 0.02-8.56 microg/g) and arsenic was 0.15 microg/g (range < 0.02-2.04 microg/g). The mean content of lead in cigarettes was 1.26 microg/g (range 0.02-6.72 microg/g) and arsenic was 0.11 microg/g (range < 0.02-0.71 micro/g). There was a large variability in lead and arsenic content among samples of tobacco and samples of cigarettes. Positive correlation between lead and arsenic contents in tobacco was found (r = 0.22; p < 0.0001). Based on our data and data from literature we compare the content of lead and arsenic in tobacco and cigarettes in other studies and discuss the influence of smoking to lead and arsenic exposure and health. In conclusion, at the same time with the implementation of tobacco use prevention programmes it is advisable to implement continuous monitoring of lead and arsenic in tobacco and cigarettes in order to reduce the health risk due to exposure of these metals.


PubMed | State University of Novi Pazar and Public Health Institute Nis
Type: Journal Article | Journal: Central European journal of public health | Year: 2013

The aim of this study was to evaluate the effects of food hygiene training of food handlers on sanitary-hygienic conditions in hospital kitchens, based on microbiological analysis of smears taken in hospital kitchens. The study was conducted in the 1995-2009 period at the Clinical Centre Nis, Serbia. The food hygiene training was conducted in February 2005, by an infection control officer. 1,076 smears in the central kitchen and 4,025 smears in distributive kitchens were taken from hands and work clothes, work surfaces, equipment, and kitchen utensils. Microbiological analysis of smears was carried out in an accredited laboratory of the Public Health Institute Nis (Serbia). A significantly lower percentage of smears with isolates of bacteria (p < 0.001) taken from hands and work clothes, work surfaces, equipment and kitchen utensils in the central and distributive kitchens was observed in the period following the food safety education programme (2005-2009). The most commonly isolated bacteria was: Enterobacter spp., Acinetobacter spp., Citrobacter spp., and E. coli. Our results confirmed that food hygiene training improved hygiene and is also an important component for the prevention of nosocomial infection.

Loading Public Health Institute Nis collaborators
Loading Public Health Institute Nis collaborators