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Lee J.G.,Korea University | Gu S.H.,Korea University | Baek L.J.,Korea University | Shin O.S.,Korea University | And 5 more authors.
Viruses | Year: 2014

The genome of Muju virus (MUJV), identified originally in the royal vole (Myodes regulus) in Korea, was fully sequenced to ascertain its genetic and phylogenetic relationship with Puumala virus (PUUV), harbored by the bank vole (My. glareolus), and a PUUV-like virus, named Hokkaido virus (HOKV), in the grey red-backed vole (My. rufocanus) in Japan. Whole genome sequence analysis of the 6544-nucleotide large (L), 3652-nucleotide medium (M) and 1831-nucleotide small (S) segments of MUJV, as well as the amino acid sequences of their gene products, indicated that MUJV strains from different capture sites might represent genetic variants of PUUV, the prototype arvicolid rodent-borne hantavirus in Europe. Distinct geographic-specific clustering of MUJV was found in different provinces in Korea, and phylogenetic analyses revealed that MUJV and HOKV share a common ancestry with PUUV. A better understanding of the taxonomic classification and pathogenic potential of MUJV must await its isolation in cell culture. © 2014 by the authors. Source


Shin S.-H.,Konkuk University | Seo H.-J.,Konkuk University | Seo H.-J.,Animal and Plant Quarantine Agency | Choi Y.-J.,Konkuk University | And 7 more authors.
Experimental and Applied Acarology | Year: 2013

A total of 1,305 ticks were collected from wild rodents captured monthly, except July and August, during 2008 at three US-ROK operated military training sites and three US military installations in Gyeonggi and Gangwon Provinces, the Republic of Korea (ROK). Ixodes nipponensis was the most frequently collected tick (n = 1,299, 99.5 %), followed by Ixodes pomerantzevi (n = 6, 0.5 %). The ticks were pooled (1-15/sample) and tested by nested polymerase chain reaction (nPCR) for spotted fever group (SFG) rickettsiae with primer sets targeting the outer membrane protein B (ompB), citrate synthase (gltA), and 17-kDa antigen gene loci. A total of 115/197 (58.4 %) pools were positive by nPCR for the outer membrane protein ompB. Nucleotide sequence analysis of 105/115 (91.3 %) ompB targeted nPCR positive products showed a high degree of similarity to Rickettsia monacensis (99.3-100 %, n = 87) and R. japonica (99.5-100 %, n = 18). From the 87 positive samples demonstrating a high degree of similarity to R. monacensis, 15 were selected and analyzed by nPCR for gltA and the 17-kDa genes. A total of 12/15 pooled samples were positive for by nPCR for gltA, with amplicons demonstrating a high degree of similarity to R. monacensis (99.3-99.7 %). A total of 13/15 pooled samples were positive by nPCR for the 17-kDa gene, with amplicons demonstrating a high degree of similarity to R. monacensis (99.4-100 %). These findings demonstrate that R. monacensis is distributed throughout Gyeonggi and Gangwon Provinces in the ROK. Furthermore, data suggest a relative high prevalence of R. monacensis in the tick, I. nipponensis. © 2013 Springer Science+Business Media Dordrecht. Source


Kim H.C.,5th Medical Detachment | Chong S.T.,5th Medical Detachment | Choi C.Y.,National Park Service | Nam H.Y.,National Park Service | And 4 more authors.
Systematic and Applied Acarology | Year: 2016

Tick surveillance of migratory birds was conducted during 2009 on Hong Island (Hong-do), Jeonnam Province, Republic of Korea. A total of 16/102 (15.8%) species of birds captured by mist net for banding were infested with ticks. A total of 143 ticks belonging to two genera and seven species—Ixodes turdus (96 ticks), Haemaphysalis flava (17), Haemaphysalis longicornis (12), Ixodes nipponensis (10), Haemaphysalis aborensis (3), Haemaphysalis hystricis (3), and Haemaphysalis doenitzi (2)—were collected from 16 bird species representing eight genera. New country and host records are provided for three uncommonly collected tick species: H. hystricis (3 nymphs; Turdus hortulorum and Zoothera dauma), H. aborensis (3 nymphs; Turdus pallidus), and H. doenitzi (1 male, 1 nymph; T. pallidus and Turdus naumanni). Most ticks (124/143; 86.7%) were collected from birds on their northward spring (March–May) migration from Southeast Asia to their breeding grounds in northeastern Asia. © Systematic & Applied Acarology Society. Source


Harrison G.F.,U.S. Army | Foley D.H.,U.S. Army | Rueda L.M.,U.S. Army | Melanson V.R.,U.S. Army | And 7 more authors.
American Journal of Tropical Medicine and Hygiene | Year: 2013

The Malaria Research and Reference Reagent Resource-recommended PLF/UNR/VIR polymerase chain reaction (PCR) was used to detect Plasmodium vivax in Anopheles spp. mosquitoes collected in South Korea. Samples that were amplified were sequenced and compared with known Plasmodium spp. by using the PlasmoDB.org Basic Local Alignment Search Tool/n and the National Center for Biotechnology Information Basic Local Alignment Search Tool/n tools. Results show that the primers PLF/UNR/VIR used in this PCR can produce uninterpretable results and non-specific sequences in field-collected mosquitoes. Three additional PCRs (PLU/VIV, specific for 18S small subunit ribosomal DNA; Pvr47, specific for a nuclear repeat; and GDCW/PLAS, specific for the mitochondrial marker, cytB) were then used to find a more accurate and interpretable assay. Samples that were amplified were again sequenced. The PLU/VIV and Pvr47 assays showed cross-reactivity with non-Plasmodium spp. and an arthropod fungus (Zoophthora lanceolata). The GDCW/PLAS assay amplified only Plasmodium spp. but also amplified the non-human specific parasite P. berghei from an Anopheles belenrae mosquito. Detection of P. berghei in South Korea is a new finding. Copyright © 2013 by The American Society of Tropical Medicine and Hygiene. Source


Suh S.J.,Kyungpook National University | Kim H.C.,5 th Medical Detachment | Chong S.T.,5 th Medical Detachment | Kim M.S.,5 th Medical Detachment | Klein T.A.,Public Health Command Region Pacific
Korean Journal of Parasitology | Year: 2015

The seasonal abundance of horse and deer flies (family Tabanidae) was analyzed using Mosquito Magnet® traps at 5 sites located near/in the demilitarized zone, northern Gyeonggi-do, Republic of Korea from late April to early October for 4 consecutive years (2010-2013). A total of 2,999 horse and deer flies (tabanids) belonging to 5 genera and 20 species were collected. Chrysops mlokosiewiczi (90.9%) was the most frequently collected, followed by Haematopota koryoensis (4.8%) and C. suavis (1.0%). The remaining 17 species comprised only of 3.3% of all species collected. C. mlokosiewiczi demonstrated bimodal peak populations during mid-June and early August, while H. koryoensis demonstrated a unimodal peak during mid-July. Overall numbers of tabanids collected were influenced by the previous year’s winter temperatures and precipitation. Population abundance was influenced by habitat with most of tabanids collected from habitats near forested areas, followed by rice paddies, and a beef farm. © 2015, Korean Society for Parasitology and Tropical Medicine. Source

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