Bao Y.,Inner Mongolia Agricultural University |
Bao Y.,Public Health College |
Wang Z.,First Affiliated Hospital |
Zhang Y.,Inner Mongolia Agricultural University |
And 7 more authors.
European Journal of Lipid Science and Technology | Year: 2012
Probiotics have been reported to play an important role in the prevention of metabolic disorders. We recently identified a novel probiotic strain Lactobacillus plantarum (L. plantarum) P-8. The objective of this study was to determine the effects of L. plantarum P-8 on lipid metabolism of rats fed with high fat diet. All experimental rats were divided into three groups: control group, model group, and L. plantarum P-8 group. Changes in serum lipid levels, hepatic lipid deposition, serum oxidative stress-related parameters, activities of liver function marker enzymes, organ indices, gut microflora, and fecal lipids were assessed. Compared with model group, L. plantarum P-8 exhibited hypolipidemic effects by lowering serum total cholesterol (TC), triglyceride (TG), and low-density lipoprotein cholesterol levels, accompanied with elevation of high-density lipoprotein cholesterol level. L. plantarum P-8 also exerted beneficial effects against high-fat diet-induced oxidative stress, curtailed the accumulation of liver lipids and protected healthy liver function. Moreover, L. plantarum P-8 was able to regulate intestinal bacteria and enhance the fecal excretion of TC, TG, and bile acid. These findings indicate that L. plantarum P-8 may represent a potential therapeutic agent for controlling hyperlipidemia. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Astrocyte elevated gene-1 (AEG-1) shRNA sensitizes Huaier polysaccharide (HP)-induced anti-metastatic potency via inactivating downstream P13K/Akt pathway as well as augmenting cell-mediated immune response
Zheng J.,Capital Medical University |
Li C.,Capital Medical University |
Wu X.,Harbin Medical University |
Liu M.,Harbin Medical University |
And 10 more authors.
Tumor Biology | Year: 2014
Astrocyte elevated gene-1 (AEG-1) is an important force in the development and progression of hepatocellular carcinoma (HCC). To extend our study, we examined here the role of AEG-1 in anti-metastatic effects of Huaier polysaccharide (HP) on the human HCC MHCC97-H cell line. AEG-1 shRNA contributed to the anti-proliferation effect of HP on MHCC97-H cells. Furthermore, results of Transwell insert chambers showed that low expression of AEG-1 could effectively facilitate HP to suppressMHCC97-H cell migration and invasion. We achieved this by reducing phosphoinositide 3- kinases (P13K) and phosphorylated Akt (pAkt) expression as well as enhancing natural killer (NK) cell activity. Taken together, our data strongly suggested that AEG-1 shRNA could block the carcinogenesis and progression of MHCC97-H cells and highlight the therapeutic potential of HP in HCC treatment, at least by part, by inhibiting the activation of the PI3K/Akt pathway and enhancing the NK cell-mediated immune response. These findings may provide a new strategy for HCC treatment. © International Society of Oncology and BioMarkers (ISOBM) 2013.
Wang Z.,Inner Mongolia Agricultural University |
Wang Z.,The First Affiliated Hospital |
Bao Y.,Inner Mongolia Agricultural University |
Bao Y.,Public health college |
And 5 more authors.
Food Biophysics | Year: 2013
We recently identified a novel probiotic strain Lactobacillus plantarum P-8 (L. plantarum P-8), which has been characterized in detail with regard to its probiotic potential. In the present study, soymilk fermented with L. plantarum P-8 was examined for its effects on diet-induced hyperlipidemia in Wistar rats. The experimental animals were divided into four groups: control group (C group), model group (M group), soymilk group (SM group) and fermented soymilk group (FSM group). The serum lipid levels, hepatic fat deposition, serum oxidative stress parameters, hepatic marker enzymes levels, organ indices, gut bacteria and fecal fat contents were analyzed. Fermented soymilk reduced the concentration of total cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C) in serum, with a significant elevation in high-density lipoprotein cholesterol (HDL) concentration. Our results also suggested the beneficial effects of fermented soymilk on the liver function, hyperlipidemia-induced oxidative stress and intestinal bacteria. Moreover, fermented soymilk could enhance the fecal excretion of TC, TG and bile acids. These findings demonstrated that soymilk fermented with L. plantarum P-8 was effective in improving the lipid metabolism in hyperlipidemic rats. The hypolipidemic effect of fermented soymilk was partly due to the inhibition of dietary fats absorption and regulation of fecal fats excretion mediated by gut bacteria. © 2012 Springer Science+Business Media New York.
Bao Y.,The Key Laboratory of Dairy Biotechnology and Bioengineering |
Bao Y.,Public Health College |
Zhang Y.,The Key Laboratory of Dairy Biotechnology and Bioengineering |
Liu Y.,The Key Laboratory of Dairy Biotechnology and Bioengineering |
And 4 more authors.
Food Control | Year: 2010
Ninety strains of Lactobacillus fermentum which were isolated from traditional dairy products of minority nationalities (53 strains from Tibet, 1 from Inner Mongolia, 7 from Yun Nan and 29 from Mongolia), were evaluated for probiotic potential with the resistance to low pH, simulated gastrointestinal juice, and bile salts. The results showed that 35 strains grew well at acid condition (pH 3.0). Eleven strains were further screened out from 35 strains with high tolerance to the simulated gastric juice (pH 2.5, 3 h of incubation). While in simulated gastric juice (pH 2.0), only F6 (isolated from traditional dairy products in Inner Mongolia) could survive to a rate of 53.7%. Moreover, among 11 Lactobacillus strains, L. fermentum F6 showed good tolerance to bile salt, while L. fermentum IMAU60151, IMAU60083, IMAU20080 and IMAU60120 were poorly presented. Further autoaggregation properties, coaggregation with pathogens and antimicrobial activity of the 11 strains were evaluated. The abilities of coaggregation with pathogens were strain-specific and independent on incubate time. All of the selected strains showed various antimicrobial activities against five food-borne pathogens. F6 possessed the highest ability of inhibiting pathogens. All selected strains showed high autoaggregation percentage after being incubated at room temperature for 20 h. Among the selected strains, F6 showed the best probiotic potential with high tolerance to simulated gastrointestinal juice and bile salts, and broad antibacterial activity. F6 had potential application in functional foods and health-associated products. © 2009 Elsevier Ltd. All rights reserved.
Fan X.-J.,Guangxi Medical University |
Qiu X.-Q.,Public Health College |
Yu H.-P.,Guangxi Medical University |
Zeng X.-Y.,Guangxi Medical University |
And 3 more authors.
Chinese Journal of Cancer Prevention and Treatment | Year: 2011
OBJECTIVE: To explore the relationship of COX-2 polymorphisms -1195G>A (rs689466) and 8473T >C (rs5275) with the susceptibility to hepatocellular carcinoma (HCC). METHODS: A hospital-based case-control study was conducted in 780 newly and histopathologically diagnosed cases with HCC and 780 cancer-free controls frequency-matched for age, sex, nationality and residence. Genotypes of COX-2 were detected using applied biosystems TaqMan genotyping platform. Unconditional Logistic regression model and χ2 test were used to evaluate the different genotypes distribution and associations with HCC risk, respectively. RESULTS: The variant genotypes of both COX-2 -1195G>A (adjusted OR = 1.32, 95%CI: 0.94-1.85) and 8473T>C (adjusted OR = 0.87, 95%CI: 0.64-1.18) polymorphisms were not significantly associated with the risk of HCC. However, the stratified analysis revealed that under a dominant model the -1195GA+AA genotypes increased the risk of HCC among the youngers (less than 55 years old, adjusted OR = 1.56, 95% CI: 1.03-2.37), 8473T>C TC+CC genotypes decreased the risk of HCC among females (adjusted OR = 0.50, 95%CI:0.25-0.99). The interactions between -1195G>A polymorphisms and age, 8473T>C polymorphisms and sex might modify the risk of HCC (P = 0.002; P = 0.007). CONCLUSIONS: COX-2 -1195G>A and 8473T>C polymorphisms do not have the independent effects for the susceptibility to HCC, but the interactions between genetic polymorphisms of -1195G>A and age, 8473T>C and sex may modify the risk of HCC.