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Turkanoglu Ozcelik A.,Middle East Technical University | Turkanoglu Ozcelik A.,Ataturk University | Can Demirdogen B.,National Public Health Agency | Demirkaya S.,Gulhane Military Medical Academy | Adali O.,Middle East Technical University
Gene | Year: 2013

Ischemic stroke is a multifactorial disease leading to severe long-term disability and it is the third leading cause of death in developed countries. Although many studies have been reported to elucidate etiological and pathological mechanisms of stroke, the genetic and molecular basis of disease remains poorly understood. Recent studies have shown that reactive oxygen species causing oxidative stress play a pivotal role in the pathogenesis of atherosclerosis that is the main cause of a group of cardiovascular diseases including ischemic stroke. In this study, we aimed to investigate the relationship between FMO3 Glu158Lys and Glu308Gly variants, and the risk of incidence of ischemic stroke in Turkish population. Two single nucleotide polymorphisms (SNPs) within the FMO3 gene were genotyped by using PCR-RFLP technique in a sample set of 245 cases and 145 controls. In the case-control analysis, no significant difference was observed between stroke patients and controls with respect to FMO3 Glu158Lys and Glu308Gly polymorphisms' genotype and allele frequency distribution. However, heterozygote 158Glu/Lys (OR = 6.110, P< 0.001) and 308Glu/Gly (OR = 6.000, P= 0.006) genotypes increase the risk of stroke 6 times in hypertensive subjects. On the other hand, the wild type genotypes 158Glu/Glu and 308Glu/Glu had 6.2-fold and 4.8-fold higher risk of ischemic stroke in obese subgroup, respectively. Our results clearly showed that the risk of hypertension-related ischemic stroke was higher in the heterozygote genotype carriers. This is the first study conducted regarding the association of FMO3 Glu158Lys and Glu308Gly genetic polymorphisms and ischemic stroke risk in Turkish population. © 2013 Elsevier B.V. Source

Kaya N.,Celal Bayar University | Kocer B.,National Public Health Agency | Devrim A.K.,Mehmet Akif Ersoy University
Fluoride | Year: 2012

Twenty Tuj sheep weighing 31±2 kg were investigated for the effect of chronic fluorosis on their serum parathyroid hormone (PTH) and calcitonin (CT) activity levels. For a period of 9 months, ten of the sheep were exposed to 4 ppm NaF (1.8 ppm F ion) in their drinking water to induce fluorosis. During the study, urinary pH gradually increased from 7.2 to 7.9. In the first 12 weeks, urinary fluoride showed a small increase from 0.9 to 1.2 ppm, but by week 38 at the end of the experiment it had risen to 16 ppm, and the serum PTH levels had decreased (p<0.01), whereas the CT levels had increased (p<0.01) compared to the control group. © 2012 The International Society for Fluoride Research Inc. Source

Can Demirdogen B.,National Public Health Agency | Ceylan O.M.,Gulhane Military Medical Academy | Isikoglu S.,Ataturk Training and Research Hospital | Mumcuoglu T.,Gulhane Military Medical Academy | Erel O.,Ataturk Training and Research Hospital
Clinical Laboratory | Year: 2014

Background: Our purpose was to determine whether total oxidant and antioxidant status, total thiol levels and activities of serum paraoxonase 1, an HDL-associated antioxidant enzyme, are related with pseudoexfoliation syndrome (PEX) and pseudoexfoliation glaucoma (PG). Methods: Serum samples from 32 PEX, 30 PG, and 32 control subjects were collected at the Giilhane Military Medical Academy, Ankara. Basal paraoxonase (PON1), salt stimulated paraoxonase (stPON1), arylesterase (ARE), and total thiol levels were measured spectrophotometrically. Total antioxidant status (TAS) and total oxidant status (TOS) were determined on an autoanalyzer. Oxidative stress index (OSI) was calculated using the TAS and TOS values. PON1 phenotypes were calculated from the ratio of stPON1 to ARE. Continuous variables were compared by independent samples t-test and one-way ANOVA, except for age which was compared using Mann-Whitney U test. Categorical variables were compared using Fisher's exact test (PON1 phenotypes) or chi-square test (gender). Results: TAS levels were significantly lower in PEX and PG patients compared to controls. TOS, OSI and total thiol levels did not differ significantly among the study groups, although there was a trend towards lower TOS and total thiol levels in patients compared to controls. PON1 activities were significantly higher in PEX patients (131.37 ± 81.20 U/L) compared to controls (95.53 ± 55.65 U/L; p = 0.046). The PON1 phenotype which is known to have high activity towards the substrate paraoxon, but low antioxidant activity (BB phenotype) was observed significantly more in PEX patients compared to controls. Conclusions: PON1 phenotypes and lack of antioxidants might play an important role in the development of PEX/PG. Source

Can Demirdogen B.,National Public Health Agency | Sahin E.,Middle East Technical University | Turkanoglu Ozcelik A.,Middle East Technical University | Bek S.,Gulhane Military Medical Academy | And 2 more authors.
Molecular Biology Reports | Year: 2012

Atherosclerosis, a major cause of ischemic stroke, may be associated with variability of triglyceride (TG) levels. Apolipoprotein A5 (APOA5) genetic polymorphisms are associated with altered TG levels. The objective of this study was to investigate the coding region polymorphisms S19W (rs3135506) and G185C (rs2075291) and the promoter region polymorphism -1131T>C (rs662799) of the APOA5 gene as risk factors for ischemic stroke in Turkish population. Study group consisted of 272 ischemic stroke patients and 123 controls. Genotypes were determined by real-time polymerase chain reaction (PCR) for S19W and PCR-restriction fragment length polymorphism analysis (PCR-RFLP) for -1131T>C and G185C. 19W allele frequency was 0.090 in stroke patients and 0.062 in controls (P = 0.191). Minor allele frequencies of -1131T>C and G185C in patients were 0.106 and 0.004, respectively, and were nearly the same in controls. Total cholesterol and LDL-cholesterol levels were significantly higher for stroke patients having at least one 19W allele compared to non-carriers. A significant difference was also found for LDL-cholesterol levels of stroke patients; higher in -1131C allele carriers compared to wild type patients. There was a trend for higher frequency of ischemic stroke among -1131C allele carrier hypertensive, diabetic or obese subjects compared to non-carriers. However, APOA5 genotypes were not associated with the risk of ischemic stroke by logistic regression analysis. The present study demonstrated that carrying rare alleles of APOA5 S19W, -1131T>C and G185C alone do not constitute a risk for ischemic stroke in the studied Turkish subjects. © 2012 Springer Science+Business Media Dordrecht. Source

Ceylan O.M.,Gulhane Military Medical Academy | Can Demirdogen B.,National Public Health Agency | Mumcuoglu T.,Gulhane Military Medical Academy | Aykut O.,National Public Health Agency
Biological Trace Element Research | Year: 2013

Pseudoexfoliation syndrome (PEX), an age-related disorder of the eye, is associated with significant ophthalmic morbidity and can lead to pseudoexfoliation glaucoma (PEG). The etiology of this disorder has not been clearly understood. Trace elements have been suggested to have roles in the pathogenesis of several disorders. This study aimed to determine whether trace element levels have a role in the development of PEX and/or PEG. Levels of zinc (Zn), copper (Cu), selenium (Se), manganese (Mn), chromium (Cr), cobalt (Co), molybdenum (Mo), nickel (Ni), vanadium (V), arsenic (As), aluminum (Al), mercury (Hg), cadmium (Cd), and strontium (Sr) were determined in serum samples of 32 cases of PEX, 30 cases of PEG, and 32 control subjects using inductively coupled plasma-mass spectrometry. Mn, Mo, and Hg concentrations were found to be significantly increased in patients with PEX. Logistic regression revealed Mn and Hg as the strongest determinants of PEX and Mo as the strongest determinant of PEG in the studied Turkish subjects. Levels of Mn, Cr, Co, Mo, Al, Hg, Sr, Ni, V, and As were determined for the first time in these ophthalmological disorders. Increased levels of serum Mn, Mo, and Hg suggest a possible role of these elements in the pathobiology of PEX. © 2013 Springer Science+Business Media New York. Source

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