Zhu Y.-L.,Peking University |
Song G.-H.,Peking University |
Liu D.-Q.,PLA Military General Hospital of Beijing |
Zhang X.,No 3 Peoples Hospital Of Chengdu |
And 16 more authors.
Chinese Journal of Cancer Research | Year: 2011
Objective: Although a new matrix formulation fentanyl has been used throughout the world for cancer pain management, few data about its efficacy and clinical outcomes associated with its use in Chinese patients have been obtained. This study aimed to assess the efficacy and safety of the new system in Chinese patients with moderate to severe cancer pain. Methods: A total of 474 patients with moderate to severe cancer pain were enrolled in this study and were treated with the new transdermal fentanyl matrix patch (TDF) up to 2 weeks. All the patients were asked to record pain intensity, side effects, quality of life (QOL), adherence and global satisfaction. The initial dose of fentanyl was 25 μg/h titrated with opioid or according to National Comprehensive Cancer Network (NCCN) guidelines. Transdermal fentanyl was changed every three days. Results: After 2 weeks. The mean pain intensity of the 459 evaluated patients decreased significantly from 5.63±1.26 to 2.03±1.46 (P<0.0001). The total remission rate was 91.29%, of which moderate remission rate 53.16%, obvious remission rate 25.49% and complete remission rate 12.64%. The rate of adverse events was 33.75%, 18.78% of which were moderate and 3.80% were severe. The most frequent adverse events were constipation and nausea. No fatal events were observed. The quality of life was remarkably improved after the treatment (P<0.0001). Conclusion: The new TDF is effective and safe in treating patients with moderate to severe cancer pain, and can significantly improve the quality of life. © Chinese Anti-Cancer Association and Springer-Verlag Berlin Heidelberg 2011.
Chen H.,Pu Ai Hospital of Wuhan City |
Chen H.,Wuhan University |
Zhang D.,Wuhan University |
Ren J.H.,Wuhan University |
Chao S.P.,Wuhan University
Iranian Journal of Pharmaceutical Research | Year: 2013
The goal of this study was to determine the effects of the L-type calcium channel blockers verapamil and diltiazem on the currents of voltage-gated potassium channel (fKv1.4ΔN), an N-terminal-deleted mutant of the ferret Kv1.4 potassium channel. Measurements were made using a two electrode voltage clamp technique with channels expressed stably in Xenopus oocytes. The fKv1.4ΔN currents displayed slow inactivation, with a half-inactivation potential of -38.38 mV and slow recovery from inactivation (τ = 1.90 seconds at -90 mV). The fKv1.4ΔN currents exhibited state-dependent blockade by both drugs, and the inhibition was frequency-, voltage-, and concentration-dependent, consistent with open channel block. Verapamil and diltiazem blocked fKv1.4ΔN currents with 50% inhibitory concentration (IC50;) values of 260.71 ± 18.50 μmol/L and 241.04 ± 23.06 μmol/L, respectively. Verapamil accelerated the C-type inactivation rate and slowed recovery of the fKv1.4Δ N channel, while shifting the steady activation curve to the right. Blockade of fKv1.4ΔN currents by diltiazem was similar to that of verapamil, but diltiazem accelerated the decay rate of inactivation of fKv1.4ΔN currents without modifying the kinetics of current activation. The present results suggest that verapamil and diltiazem accelerate the C-type inactivation and slow the recovery of the fKv1.4ΔN channel in the open state. © 2013 by School of Pharmacy.
Zhang D.,Wuhan University |
Xu M.,Wuhan University |
Quan L.,Wuhan University |
Yang Y.,Wuhan University |
And 2 more authors.
Physics in Medicine and Biology | Year: 2015
It is crucial in high intensity focused ultrasound (HIFU) therapy to detect the tumor precisely with less manual intervention for enhancing the therapy efficiency. Ultrasound image segmentation becomes a difficult task due to signal attenuation, speckle effect and shadows. This paper presents an unsupervised approach based on texture and boundary encoding customized for ultrasound image segmentation in HIFU therapy. The approach oversegments the ultrasound image into some small regions, which are merged by using the principle of minimum description length (MDL) afterwards. Small regions belonging to the same tumor are clustered as they preserve similar texture features. The mergence is completed by obtaining the shortest coding length from encoding textures and boundaries of these regions in the clustering process. The tumor region is finally selected from merged regions by a proposed algorithm without manual interaction. The performance of the method is tested on 50 uterine fibroid ultrasound images from HIFU guiding transducers. The segmentations are compared with manual delineations to verify its feasibility. The quantitative evaluation with HIFU images shows that the mean true positive of the approach is 93.53%, the mean false positive is 4.06%, the mean similarity is 89.92%, the mean norm Hausdorff distance is 3.62% and the mean norm maximum average distance is 0.57%. The experiments validate that the proposed method can achieve favorable segmentation without manual initialization and effectively handle the poor quality of the ultrasound guidance image in HIFU therapy, which indicates that the approach is applicable in HIFU therapy. © 2015 Institute of Physics and Engineering in Medicine.
Pan R.,Huazhong University of Science and Technology |
Rong Z.,Huazhong University of Science and Technology |
She Y.,Indiana University |
Cao Y.,Pu Ai Hospital of Wuhan City |
And 2 more authors.
Pediatric Research | Year: 2012
BackgroundNeonatal hypoxia-ischemia (HI) remains a major cause of severe brain damage and is often associated with high mortality and lifelong disability. Immature brains are extremely sensitive to HI, shown as prolonged mitochondrial neuronal death. Sodium pyruvate (SP), a substrate of the tricarboxylic acid cycle and an extracellular antioxidant, has been considered as a potential treatment for hypoxic-ischemic encephalopathy, but its effects have not been evaluated in appropriate animal models for hypoxic-ischemic encephalopathy.MethodsThis investigation used primary cortical neuron cultures derived from neonatal rats subjected to oxygen and glucose deprivation (OGD) and a well-established neonatal rat HI model.ResultsHI caused brain tissue loss and impaired sensorimotor function and spatial memory whereas SP significantly reduced brain damage and improved neurological performance. These neuroprotective effects of SP are likely the result of improved cerebral metabolism as demonstrated by maintaining adenosine triphosphate (ATP) levels and preventing an increase in intracellular reactive oxygen species (ROS) levels. SP treatment also decreased levels of Bax, a death signal for immature neurons, blocked caspase-3 activation, and activated a key survival signaling kinase, Akt, both in vitro and in vivo.ConclusionSP protected neonatal brain from hypoxic-ischemic injury through maintaining cerebral metabolism and mitochondrial function. © 2012 International Pediatric Research Foundation, Inc.
Chen M.,Pu Ai Hospital of Wuhan City |
Kan W.-S.,Pu Ai Hospital of Wuhan City |
Fang Z.-H.,Pu Ai Hospital of Wuhan City |
Zheng Q.,Pu Ai Hospital of Wuhan City |
Xu M.-C.,Pu Ai Hospital of Wuhan City
Chinese Journal of Tissue Engineering Research | Year: 2013
BACKGROUND: Life quality of patients after total hip arthroplasty will affected by various factors, and the factors that can affect the treatment effect after replacement should be analyzed in order to take the specific measures to intervene. OBJECTIVE: To investigate the relative factors that affect the treatment effect of initial total hip arthroplasty. METHODS: The clinical data of 352 patients treated with initial total hip arthroplasty were retrospectively analyzed, and the factors that affect the scoring were determined according to the Harris score. The relationship between various factors and the treatment of total hip arthroplasty was evaluated and analyzed through chi-square test and Spearman classification. Comparative analysis was performed to compare the results of relative articles and researches. RESULTS AND CONCLUSION: Age, body mass index, hip rotation center, femoral eccentricity and post-operative rehabilitation training of the patients with initial total hip arthroplasty could affect the medium-term results after total hip arthroplasty. While the influence of preoperative diagnosis, surgical approach, patient gender and type of prosthesis on the treatment effect of total hip arthroplasty was not obvious. Adequate attention to these factors, correct prevention and scientific treatment of a variety of factors that may affect the recovery of joint function, all these effort are the prerequisites for the surgeons to improve satisfaction of the patients.
Pei L.,Huazhong University of Science and Technology |
Zhang J.,Pu Ai Hospital of Wuhan City |
Zhao F.,Huazhong University of Science and Technology |
Su T.,Huazhong University of Science and Technology |
And 4 more authors.
British Journal of Anaesthesia | Year: 2011
Background. Annexin 1 (ANXA1) has analgesic effects in inflammatory pain. We aimed to investigate the anti-nociceptive role of ANXA1, at the dorsal root ganglion (DRG) level, through an interaction with formyl-peptide-receptor-like 1 (FPR2/ALX). Methods. Inflammatory pain was evoked by injecting complete Freunds adjuvant (CFA, 50 μl) into the hindpaw of male SpragueDawley rats. The distribution of ANXA1 and FPR2/ALX in L4/5 DRGs was evaluated by immunofluorescence. The expression of ANXA1 was measured by western blot. The involvement of FPR2/ALX in the anti-nociception of ANXA1 was investigated by thermal (irradiant heat) and mechanical (von Frey filament) pain tests with intrathecal (i.t.) ANXA1-derived peptide (Anxa1 2-26), FPR2/ALX agonist 5(S)-6(R)-7-trihydroxyheptanoic-acid-methyl-ester (BML-111), and antagonist N-t-Boc-Phe-Leu-Phe-Leu-Phe (Boc1). Results. ANXA1 and FPR2/ALX localized in the satellite glial cells and neurones in L4/5 DRGs. CFA treatment (n=20) increased ANXA1 expression in L4/5 DRGs within 7 days (P<0.01). I.T. Anxa1 2-26 (20 and 100 μg l -1) and BML-111 (10 and 100 nmol) reduced CFA-induced thermal and mechanical nociception within 48 h (n=40) (P<0.05). However, i.t. Boc1 10 μg intensified inflammatory pain (P<0.05) and reversed the anti-nociceptive effect of Anxa1 2-26 (n=25) (P<0.05). Moreover, ANXA1 expression increased in L4/5 DRGs after i.t. Anxa1 2-26 (20 μg l -1) (P<0.05) and BML-111 (10 nmol) (P<0.01) but decreased after i.t. Boc1 (10 and 100 μg) alone (P<0.01) or Boc1 (10 μg) co-injection with Anxa1 2-26 (20 μg μl -1) (P<0.05). Conclusions. Endogenous ANXA1 expression at the DRG level is involved in CFA-induced inflammatory pain, and i.t. ANXA1 20 μg μl -1 produces its anti-nociceptive effect through FPR2/ALX. © The Author . Published by Oxford University Press on behalf of the British Journal of Anaesthesia. All rights reserved.