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Rohtak, India

Yadav S.,Pt. B.D. Sharma UHS | Yadav A.,Pt. B.D. Sharma UHS | Sharma M.,Pt. B.D. Sharma UHS | Chaudhary U.,Pt. B.D. Sharma UHS
International Journal of Pharma and Bio Sciences | Year: 2010

The problem of infection has been persistent in the surgical world even after the introduction of antibiotics. Pathogens that infect surgical wounds can be part of normal flora or acquired from the hospital environment or other infected patients. Staphylococcus aureus, being the normal microbial flora of the skin is one of the commonest causes of wound infection. A total of 1088 pus samples from surgical wounds were received in the Department of Microbiology, Pt.B.D.S, UHS, Rohtak over a period of one year. The isolates were identified using standard laboratory procedures. All the isolates were tested for susceptibility to various commonly used antibiotics and screened for oxacillin susceptibility according to CLSI guidelines.Out of 1088 pus samples received, 833(76.5%) were culture positive. S.aureus accounted for 10.6% of surgical wound infections. Methicillin resistance was documented in 54 (60.6%) of the S.aureus isolates. Highest efficacy was observed with linezolid (100%). The hospital acquired surgical site infection is alarming. Hospital disinfection and treatment protocols should be practiced.

Sharma M.,Pt. B.D. Sharma UHS | Yadav S.,Pt. B.D. Sharma UHS | Aparna,Pt. B.D. Sharma UHS | Chaudhary U.,Pt. B.D. Sharma UHS
International Journal of Pharma and Bio Sciences | Year: 2011

Candida spp. are known to be the most common fungal pathogens isolated from blood cultures of neonates. The present study was undertaken to know the prevalence of candidemia in neonates and their antifungal susceptibility pattern. Candida isolates were speciated by using germ tube, carbohydrate assimilation and fermentation tests, CHROM agar and Dalmau plate culture method. Antifungal susceptibility testing to amphotericin B and fluconazole was done using E-test. A total of 56 Candida isolates were obtained over a period of one year from neonates. Of these 42 isolates were non-albicans Candida. All the isolates were sensitive to amphotericin B. Fluconazole was more commonly observed in non-albicans Candida. Candida speciation as well as antifungal susceptibility pattern need to be studied, especially in light of increasing drug resistance and newer antifungals to be evaluated for therapy.

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