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Dahiya K.,78 28 Bharat Colony | Kulshrestha M.R.,78 28 Bharat Colony | Bansal P.,78 28 Bharat Colony | Ghalaut V.S.,78 28 Bharat Colony | And 3 more authors.
Hypertension in Pregnancy | Year: 2015

Objective: Pre-eclampsia is associated with ischemia and increased oxidative stress, which may lead to modification of plasma albumin to ischemia modified albumin (IMA). Methods: IMA levels were estimated in cord blood of 30 newborns born to pre-eclamptic mothers and compared with 30 normal newborns. IMA was estimated colorimetrically and the results were compared statistically. Results: The levels of IMA were found to be significantly higher (p<0.001) in newborns born to pre-eclamptic mothers (0.835±0.02 ABSU) as compared to those born to normal mothers (0.325±0.01 ABSU). Conclusion: IMA may act as a marker of ischemia and oxidative stress in newborns delivered to pre-eclamptic mothers. © 2015 Informa Healthcare USA, Inc. Source

Singh V.,Pt. B.D. Sharma PGIMS | Kaushik M.,Jubilant Clinsys Ltd | Dahiya K.,Pt. B.D. Sharma PGIMS | Ghalaut P.S.,Pt. B.D.Sharma PGIMS | And 2 more authors.
Journal of Clinical and Diagnostic Research | Year: 2012

Introduction: Many acquired enzymopathies have been reported in patients with acute leukaemia. Oxidative stress is also found to be increased in acute leukaemia, which can be indicated by the increased malondialdehyde (MDA) levels. Malondialdehyde itself may lead to the alteration of the enzyme, pyruvate kinase. Therefore, this study was planned to estimate the levels of erythrocytic pyruvate kinase and malondialdehyde in patients of acute leukaemia before and after chemotherapy. Methods: This study was conducted on 20 patients of acute myeloid leukaemia, 20 patients of acute lymphoblastic leukaemia and 20 healthy controls. The levels of pyruvate kinase and malondialdehyde were estimated in the haemolysate in the controls and in the patients at the time of diagnosis. These levels were also estimated in the patients after six weeks of chemotherapy or remission, whichever was earlier. The results were compared statistically. Results: The levels of erythrocytic pyruvate kinase were found to be decreased significantly in the acute leukaemia patients as compared to controls and to be increased after chemotherapy, while the levels of erythrocytic malondialdehyde were found to be increased significantly in these patients as compared to the controls and to be decreased after chemotherapy. The levels were not statistically significantly different in acute myeloid and acute lymphoblastic leukaemia. Conclusion: The erythrocytic pyruvate kinase and the malondialdehyde levels may help in the diagnosis, for asessing the severity and for the monitoring of the acute leukaemia patients. Source

Aggarwal K.,Kalinga Institute of Medical science | Seth S.,Pt. B.D.Sharma PGIMS | Dahiya K.,Pt. B.D.Sharma PGIMS | Aggarwal H.K.,Pt. B.D.Sharma PGIMS | Aggarwal M.K.,Kalinga Institute of Medical science
Biomedical Research | Year: 2012

Existing biochemical markers are not suitable for assessing early myocardial ischemia particularly in first 2-6 hours of ischemia i.e. before setting in of irreparable myocardial damage. Recently ischemia modified albumin (IMA) has emerged as the earliest cardiac marker. Therefore, this study was planned to estimate IMA levels in patients of acute chest pain with or without cardiac cause. One hundred patients of acute chest pain (50 patients with cardiac cause and 50 patients with non-cardiac cause) were selected for the study and compared with 50 age and sex matched healthy controls. IMA, creatine kinase- MB (CK-MB), Troponin I, routine biochemical investigations, hemoglobin and total leucocyte count were estimated in these patients. The results were analysed statistically. IMA levels were found to be significantly elevated in patients of chest pain with myocardial ischemia as compared to the other two groups. A significant positive correlation was found between IMA levels & CKMB levels (r = +0.713, p value<0.001) and IMA levels & troponin I levels (r = +0.823, p value<0.001) but a poor correlation was observed between IMA levels and SGOT levels (r = -.012, p value>0.05). Thus we conclude that IMA assay is a sensitive but not very specific marker for early detection of myocardial ischemia. IMA can be combined with other biochemical markers like CK-MB and Troponin I to increase the specificity. Other non myocardial ischemic conditions should be kept in mind while interpreting IMA values. Source

Singla P.,Pt. B.D.Sharma PGIMS | Sikka R.,Pt. B.D.Sharma PGIMS | Deeep A.,Pt. B.D.Sharma PGIMS | Deep G.,General Hospital | Chaudhary U.,Microbiology
Journal of Clinical and Diagnostic Research | Year: 2014

Background: Acinetobacter baumannii is an important cause of health care associated infections which are difficult to control and treat, because of widespread antimicrobial resistance which is possessed by this organism. Aims: The aim of the present study was to know the prevalence of ESBLs and AmpC β-lactamases in clinical isolates of Acinetobacter spp. which were cultured from various clinical specimens by using different phenotypic methods. Settings and design: Study was conducted over a period of one year at the Microbiology Department of a tertiary care teaching hospital. A total of 100 consecutive, non-duplicate strains of Acinetobacter species which were isolated from various clinical samples were included. Materials and Methods: All the isolates were identified by standard microbiological procedures and antimicrobial susceptibility testing was done by Kirby-Bauer disc diffusion technique. Isolates which showed reduced susceptibilities to third generation cephalosporins were tested for ESBL production by CLSI double disc synergy method and also by using sulbactam as an inhibitory agent. Isolates which showed reduced susceptibilities to cefoxitin were tested for AmpC detection by doing AmpC disc test. Statistical Analysis: SPSS, version 17 was used to calculate p-value. If the p-value was <0.05, it was considered to be significant. Results: Out of 100 isolates, 82 were Acinetobacter baumannii and 18 were Acinetobacter lwoffii. ESBL were mentioned in 4% of the Acinetobacter isolates and in 77% of the isolates by using clavulanic acid and sulbactam as inhibitory agents respectively. AmpC β-lactamase production was detected in 60% isolates of Acinetobacter spp. Co-production of both ESBL and AmpC enzymes were seen in 29% of the Acinetobacter strains. Conclusion: Failure in detecting β-lactamases contributes to their uncontrolled spread and therapeutic failures. Hence, these β-lactamases should be detected routinely and they should be reported to clinicians in time, so that inappropriate use of antibiotics can be stopped in time. Source

Ghalaut V.S.,Pt. B.D.Sharma PGIMS | Sangwan L.,Pt. B.D.Sharma PGIMS | Dahiya K.,Pt. B.D.Sharma PGIMS | Ghalaut P.S.,Pt. B.D.Sharma PGIMS | And 2 more authors.
Journal of Oncology Pharmacy Practice | Year: 2012

Nitric oxide (NO) is involved in different stages of malignancies. Increased levels of NO have been reported in different leukemias. Imatinib is the preferred drug for the treatment of chronic myeloid leukemia (CML). Turmeric powder contains curcumin which has anti-leukemic property and also decreases NO synthesis. This study was conducted on fifty patients of CML divided into two groups, group A receiving imatinib alone and group B receiving turmeric powder along with imatinib for six weeks. Nitric oxide levels were estimated in these patients before and after receiving therapy and were analyzed statistically. Nitric oxide levels were found to be significantly decreased in both the groups, but more significantly in group B after receiving the respective treatments. Thus, curcumin acts as an adjuvant to imatinib in decreasing the NO levels and may help in the treatment of CML patients. © The Author(s) 2011. Source

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