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Amavet P.S.,National University of Santa | Amavet P.S.,University of Buenos Aires | Amavet P.S.,Proyecto Yacare Laboratorio Of Zoologia Aplicada Anexo Vertebrados Fhuc Unl Maspyma | Vilardi J.C.,University of Buenos Aires | And 6 more authors.
Amphibia Reptilia | Year: 2012

The knowledge about reproductive strategies of a species contributes to their conservation. Multiple paternity is a reproductive behavior increasing effective population size, which could increase genetic diversity particularly in populations submitted to bottlenecks events. In Argentina, wild populations of Caiman latirostris are subject of a management plan devoted to their preservation and sustainable utilization based on its commercial interest. This program started in response to the evident numeric reduction of the populations, as a consequence of hunting pressure and habitat modification; it had a remarkable success in population recovery allowing the commercial use of C. latirostris. Data on reproductive behavior of C. latirostris are limited because mating occurs in the water and the information about their genetic diversity is scarce too. Our specific aims were to study the mating system and population genetic structure applying microsatellite markers in twelve C. latirostris families. The obtained results showed highly significant difference among populations and a lack of correspondence between geographical distance and genetic differentiation suggesting that populations of C. latirostris represent unstable metapopulations. In the paternity analysis was detected more than one father in two nests, which could be explained by capacity of storage sperm, proposed in females of a related species. The behavior of multipaternity could contribute to maintain viable populations of C. latirostris, since the maintenance of genetic variability within populations could help increase their capacity to respond to selective pressure. Further studies employing genetic and behavioral framework are needed to better understand the reproductive biology of C. latirostris. © 2012 Koninklijke Brill NV, Leiden. Source


Larriera A.,Proyecto Yacare Laboratorio Of Zoologia Aplicada Anexo Vertebrados Fhuc Unl Maspyma | Siroski P.A.,Proyecto Yacare Laboratorio Of Zoologia Aplicada Anexo Vertebrados Fhuc Unl Maspyma | Siroski P.A.,CONICET | Rueda E.C.,Laboratorio Of Genetica | And 6 more authors.
Amphibia Reptilia | Year: 2015

Wild populations of Caiman latirostris are subject to sustainable use programs in Argentina, becoming a species with important impact in the regional economy, based in their skin and meat. Genetic studies are fundamental to acquire information on important parameters for conservation and management, which may be obtained from analysis of molecular markers. Some microsatellites have been previously isolated in this species, but due to some difficulties in using them, we obtained new ones using Next Generation Sequencing approach. This study reports eight new microsatellites for C. latirostris and tests their utility in a related species, Caiman yacare, with successful application in population genetics and mating systems studies. In addition, we shared data about a novel and fast bioinformatics tool to find microsatellites and to design their corresponding primers. Source


Imhoff C.,Laboratorio Of Genetica | Imhoff C.,CONICET | Giri F.,CONICET | Siroski P.,CONICET | And 4 more authors.
Journal of Morphology | Year: 2016

The study of the cephalic shape of crocodilian is relevant in the fields of ecology, systematics, evolution, and conservation. Therefore, the integration of geometric analysis within quantitative genetics allows the evaluation of the inheritable shape components. In this study, the dorsal cephalic region of 210 Caiman latirostris hatchlings was analyzed from seven populations in Santa Fe, Argentina, to detect intra-, and inter-population phenotypic variability, and to determine the heritability of biological shape and size, using newly available geometric morphometric tools. The principal component analysis showed two configurations of cephalic shape that could be related to sexual dimorphism. In the canonical variate analysis, Procrustes distances between groups indicated that there are differences in shape among populations. Furthermore, the method of partial least squares indicated a covariation between cephalic shape and environmental variables. Regarding to CS of the skull we found significant differences among populations, moreover the partial least squares was also significant. Estimates of the heritability of shape and size were high, indicating that the components of these features are susceptible to the selection. © 2016 Wiley Periodicals, Inc. Source


Siroski P.A.,Proyecto Yacare Laboratorio Of Zoologia Aplicada Anexo Vertebrados Fhuc Unl Maspyma | Siroski P.A.,CONICET | Poletta G.L.,Proyecto Yacare Laboratorio Of Zoologia Aplicada Anexo Vertebrados Fhuc Unl Maspyma | Poletta G.L.,CONICET | And 4 more authors.
Acta Herpetologica | Year: 2014

Chitin is an abundant bio-polymer present as a structural component of many organisms such as arthropods, nematodes, mollusks, insects, and fungi, among others. Chitinolytic enzymes are synthesized for organisms to defend themselves against chitin-containing pathogens. Chitotriosidase (CHT) is a chitinase enzyme and one of the main proteins secreted by activated macrophages. It plays an important role in mechanisms of immunity by hydrolyzing chitin, thus protecting against chitin-containing pathogens. In this study, CHT was detected in Caiman latirostris plasma, and characterized under laboratory controlled conditions of temperature, reaction time, plasma concentration, pH and salinity. The results complement other immunological studies performed in caimans and demonstrate that they possess an efficient and well-developed immune system that resists the attack of some pathogens. Based on the current knowledge of the properties and homologies of CHT, it would be highly valuable to evaluate its possible therapeutic application in the veterinary clinical setting. © Firenze University Press. Source

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