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Simoneau-Buessinger E.,University of Valenciennes and HainautCambresis | Leteneur S.,University of Valenciennes and HainautCambresis | Toumi A.,University of Valenciennes and HainautCambresis | Dessurne A.,Provincial High School of Hainaut-Condorcet | And 3 more authors.
PLoS ONE | Year: 2015

During maximal contractions, the sum of forces exerted by homonymous muscles unilaterally is typically higher than the sum of forces exerted by the same muscles bilaterally. However, the underlying mechanism(s) of this phenomenon, which is known as the bilateral strength deficit, remain equivocal. One potential factor that has received minimal attention is the contribution of body adjustments to bilateral and unilateral force production. The purpose of this study was to evaluate the plantar-flexors in an innovative dynamometer that permitted the influence of torque from body adjustments to be adapted. Participants were identically positioned between two setup configurations where torques generated from body adjustments were included within the net ankle torque (locked-unit) or independent of the ankle (open-unit). Twenty healthy adult males performed unilateral and bilateral maximal voluntary isometric plantar-flexion contractions using the dynamometer in the open and locked-unit mechanical configurations. While there was a significant bilateral strength deficit in the locked-unit (p = 0.01), it was not evident in the open-unit (p = 0.07). In the locked-unit, unilateral torque was greater than in the open-unit (p<0.001) and this was due to an additional torque from the body since the electromyographic activity of the agonist muscles did not differ between the two setups (p>0.05). This study revealed that the mechanical configuration of the dynamometer and then the body adjustments caused the observation of a bilateral strength deficit. © 2015 Simoneau-Buessinger et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Nguyen V.T.P.,Laboratory of Hematology and Haemostasis | Vancles P.,Provincial High School of Hainaut-Condorcet | Rozen L.,Laboratory of Hematology and Haemostasis | Noubouossie D.,Laboratory of Hematology and Haemostasis | Demulder A.,Laboratory of Hematology and Haemostasis
Immuno-Analyse et Biologie Specialisee | Year: 2013

The aim of this study is to evaluate the analytical performance of the new Sysmex XN-2000® analyzer in our routine laboratory. Overall, 798 adult and 340 pediatric blood samples were analyzed during a period of 3weeks on the Sysmex XN-2000® and Advia 2120i® as well as 100 body fluids. Analytical parameters as within-run and inter-day precisions, carry-over, linearity, limit of quantification, stability are excellent for the complete blood count (CBC) and correlation with Advia results shows high concordance between most parameters, except for the monocytes counts which are systematically higher with the Sysmex XN-2000®. The body fluids module shows high precision (CV<10%) for WBC with negligible carry-over and excellent linearity. However, the BF module cannot give a number of RBC below 1000μL. RBC results are only provided in interval of 103/μL cells with no intermediate counts. We concluded that the Sysmex XN-2000 provides highly reliable results. However, in our view, improvement of RBC count is mandatory and impairs full-automated counts of this kind of samples. © 2013 .

Rouse G.W.,University of California at San Diego | Lanterbecq D.,University of Mons | Lanterbecq D.,Provincial High School of Hainaut-Condorcet | Summers M.M.,University of California at San Diego | Eeckhaut I.,University of Mons
Journal of Natural History | Year: 2016

Mesomyzostoma Remscheid, 1918 currently includes three described species that live in the coelom and/or gonads of comatulid crinoids: Mesomyzostoma reichenspergeri Remscheid, 1918, Mesomyzostomakatoi Okada, 1933 and Mesomyzostomalanterbecqae Summers and Rouse, 2014 in Summers, Al-Hakim et al. 2014. Here we describe four new species of Mesomyzostoma and assess their phylogenetic relationships using 18S rRNA, cytochrome oxidase subunit I and 16S rRNA sequence data. We also designate a neotype for M. katoi as the original types appear to be lost. We record M. reichenspergeri from the Australian Great Barrier Reef and from northern Papua New Guinea, but samples from the type locality (Aru Islands, Indonesia) and previously recorded host are needed for confirmation. The new species of Mesomyzostoma are one Japanese species: Mesomyzostomaokadai sp. nov., and three Australian species: Mesomyzostomalobus sp. nov., Mesomyzostomaleukos sp. nov. and Mesomyzostomabotulus sp. nov. The first infects the coelom of crinoid arms and pinnules, and the other three are found in crinoid oral discs. We also record M. leukos sp. nov. and M. botulus sp. nov. from Papua New Guinea. Phylogenetic analyses suggest that M. okadai sp. nov. is the sister group to all other Mesomyzostoma. © 2015 Taylor & Francis.

Aucouturier J.,Lille 2 University of Health and Law | Boissiere J.,Lille 2 University of Health and Law | Pawlak-Chaouch M.,Lille 2 University of Health and Law | Cuvelier G.,Provincial High School of Hainaut-Condorcet | Gamelin F.-X.,Lille 2 University of Health and Law
Nitric Oxide - Biology and Chemistry | Year: 2015

Abstract Dietary nitrate (NO3-) supplementation has been shown to increase exercise tolerance and improve oxidative efficiency during aerobic exercise in healthy subjects. We tested the hypothesis that a 3-day supplementation in beetroot juice (BJ) rich in NO3- would improve the tolerance to supramaximal intensity intermittent exercise consisting of 15-s exercise periods at 170% of the maximal aerobic power interspersed with 30-s passive recovery periods. The number of repetitions completed before reaching volitional exhaustion was significantly higher in the BJ than in the placebo condition (26.1 ± 10.7 versus 21.8 ± 8.0 respectively, P < 0.05). In contrast to previous findings during exercise performed at intensity below the peak oxygen uptake (VO2peak), oxygen uptake (VO2) was unaffected (BJ: 2735 ± 345 mL kg-1 min-1 vs. placebo: 2787 ± 346 mL kg-1 min-1, NS). However, the Area Under the Curve for microvascular total hemoglobin (AUC-THb) in the vastus lateralis muscle assessed by near infrared spectroscopy during 3 time-matched repetitions was significantly increased with NO3- supplementation (BJ: 9662 ± 1228 a.u. vs. placebo:8178 ± 1589a.u.; P < 0.05). Thus, increased NO3- (BJ: 421.5 ± 107.4 μM vs placebo:39.4 ± 18.0 μM) and NO2- (BJ: 441 ± 184 nM vs placebo: 212 ± 119 nM) plasma levels (P < 0.001 for both) are associated with improved muscle microvascular Red Blood Cell (RBC) concentration and O2 delivery during intense exercise, despite no effect on resting femoral artery blood flow, and vascular conductance. Maximal voluntary force during an isometric leg extensor exercise, and blood lactate levels were also unaffected by NO3- supplementation. To conclude, dietary NO3- supplementation enhances tolerance to exercise at supramaximal intensity, with increased microvascular total RBC concentration in the working muscle, in the absence of effect on contractile function and resting hemodynamic parameters. © 2015 Elsevier Inc.

Pawlak-Chaouch M.,Lille 2 University of Health and Law | Boissiere J.,Lille 2 University of Health and Law | Gamelin F.X.,Lille 2 University of Health and Law | Cuvelier G.,Provincial High School of Hainaut-Condorcet | And 2 more authors.
Nitric Oxide - Biology and Chemistry | Year: 2016

Background Recent randomized controlled trials have suggested that dietary nitrate (NO3 -), found in beetroot and other vegetables, and inorganic NO3 - salts decrease metabolic rate under resting and exercise conditions. Objective Our aim was therefore to determine from a systematic review and meta-analysis whether dietary NO3 - supplementation significantly reduces metabolic rate, expressed as oxygen uptake (VO2), under resting and exercise conditions in healthy humans and those with cardiorespiratory diseases. Design A systematic article search was performed on electronic databases (PubMed, Scopus and Web of Science) from February to March 2015. The inclusion criteria included 1) randomized controlled trials; 2) studies reporting the effect of NO3 - on VO2 under resting and/or exercise conditions; 3) comparison between dietary NO3 - supplementation and placebo. Random-effects models were used to calculate the pooled effect size. Results Twenty nine randomized placebo-controlled trials were included in the systematic review, and 26 of which were included in the meta-analysis. Dietary NO3 - supplementation significantly decreases VO2 during submaximal intensity exercise [-0.26 (95% IC: -0.38, -0.15), p < 0.01], but not in the sub-analysis of subjects with chronic diseases [-0.09 (95% IC: -0.50, 0.32), p = 0.67]. When data were separately analyzed by submaximal intensity domains, NO3 - supplementation reduces VO2 during moderate [-0.29 (95% IC: -0.48,-0.10), p < 0.01] and heavy [-0.33 (95% IC: -0.54,-0.12), p < 0.01] intensity exercise. When the studies with the largest effects were excluded from the meta-analysis, there is a trend for a VO2 decrease under resting condition in dietary NO3 - supplementation [-0.28 (95% IC: -0.62, 0.05), p = 0.10]. Conclusion Dietary NO3 - supplementation decreases VO2 during exercise performed in the moderate and heavy intensity domains in healthy subjects. The present meta-analysis did not show any significant effect of dietary NO3 - supplementation on metabolic rate in subjects with chronic diseases, despite enhanced exercise tolerance. © 2016 Elsevier Inc. All rights reserved.

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