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Ahmad M.S.,Provincial Diagnostic Laboratory | Maqbool A.,University of Veterinary and Animal Sciences | Mahmood-ul-Hassan M.,University of Veterinary and Animal Sciences | Mushtaq-ul-Hassan M.,University of Veterinary and Animal Sciences | Anjum A.A.,Government College University at Faisalabad
Journal of Animal and Plant Sciences | Year: 2011

Babesiosis was recorded in 2.3 percent of the rodents in the Lahore - the second most populated city of Pakistan and the capital of Punjab. The highest prevalence was recorded during the August at all the four structures sampled for rats and mice. It was 8.7 percent at Residential houses (RH) 10.7 percent at flour mills (FM) and fruit/vegetable shops (F/VS), and 6.7 percent at departmental stores and grocery shops (DS/GS). Inter-structure variation in the prevalence rate was statistically non-significant. Rodents captured from November to April had no infection. Sex-wise infection rate was statistically different in all the structures. Infection rate was higher in mice (2.4 percent) than rats (2.3 percent) but the difference was non-significant. Findings suggest that infected rodents may become a general health hazard and suitable measures required to eradicate them. Source


Ahmad M.S.,Provincial Diagnostic Laboratory | Maqbool A.,University of Veterinary and Animal Sciences | Mahmood-ul-Hassan M.,University of Veterinary and Animal Sciences | Mushtaq-ul-Hassan M.,University of Veterinary and Animal Sciences | Anjum A.A.,Government College University at Faisalabad
Journal of Animal and Plant Sciences | Year: 2011

A total of 3600 rodents were live-trapped from three different localities (1200 each) of the Lahore Metropolitans Allama Iqbal Town, Walled City and Railway Station and adjacent areas, and scanned for the prevalence of Capillaria hepatica. In each of these four types of structures were sampled for rats and mice namely residential houses (RH), flour mills (FM), fruit/vegetable shops (F/VS) and departmental stores/grocery shops (DS/GS). Majority of the trapped rodents included house rats Rattus rattus (n = 3190) with a few house mice Mus musculus (n = 410). Hepatic capillariasis was recorded in 7.0% of the rodents in Lahore city. The prevalence was statistically higher in females than in males and in adults than young animals. A significant difference was found in the infection rate between rats and mice. Similarly the infection rate varied significantly both monthly and seasonally and the peak prevalence was recorded during summer (10.8%). Inter-structure infection rate was non-significant. Source


Ahmad M.S.,Provincial Diagnostic Laboratory | Maqbool A.,University of Veterinary and Animal Sciences | Mahmood-ul-Hassan M.,University of Veterinary and Animal Sciences | Mushtaq-ul-Hassan M.,Government College University at Faisalabad | Anjum A.A.,University of Veterinary and Animal Sciences
Journal of Animal and Plant Sciences | Year: 2012

An endeavor was made to assess the prevalence of Toxoplasma infection in humans and in rodents captured from the corresponding areas of Lahore using the Latex Agglutination Test (LAT). Sera samples of Rattus rattus (n=210), Mus muscular (n=90) and human beings (n=300) were screened for antibodies against T. gondii. Highest percentage of positive cases (58.57%) was recorded in R.rattus followed by M.muscularus (36.66%) and lowest in human beings (11.33%). Sera samples from human beings showed significant difference in relation to positive level of antibodies against T. gondii at different age groups. Highest percent prev lence of antibodies (28%) was observed in human sera samples collected from more than 51 years of age group followed by 41-50 years age group (17.33%). Remaining two age groups (21-30 and 31-40 years) were negative for anti-T. gondii antibodies. Anti-T. gondii antibodies were detected in sera of rodents and human beings residential of same localities. So, rodents can play role in the transmission of T. gondii to human beings. Source


Latif A.A.,Lahore College for Women University | Tanveer A.,University of Punjab | Anjum A.A.,University of Veterinary and Animal Sciences | Ali M.A.,University of Veterinary and Animal Sciences | And 3 more authors.
Journal of Animal and Plant Sciences | Year: 2013

Polypeptide analysis of purified hydatid cyst fluid (HCF) from ruminants (sheep, goat, cattle, buffalo and camel) and human beings was performed by sodium dodecyl sulphate polyacrylamide gel (12%) electrophoresis (SDS-PAGE) under reduced conditions. HCFs from different species revealed variable segregation pattern of proteins not only within the different hosts of the parasites but also in different types of HCF. In fertile HCF, five to three protein bands were observed in different intermediate hosts of the parasite viz., buffalo (269, 166, 89, 59 and 25kDa), goat (46, 29, 22 and 18kDa), sheep (209, 138 and 63kDa), cattle (269, 89 and 59kDa), camel (195, 166 and 123kDa) and human beings (195, 138, 21 and 6kDa). In sterile HCF of different species, less number of protein bands was found as compared to fertile cysts. The two protein bands were observed in sterile HCF collected from camel (195 and 141kDa), cattle (27 and 18 kDa), buffalo (43 and 27kDa) and human being (30 and 21kDa), whereas only one polypeptide band found in sheep (123kDa) and goat (78kDa). SDS-PAGE analysis of hydatid cyst fluid collected from different intermediate hosts of Echinococcus granulosus exhibited variable profiles of proteins. The present study suggests that SDS-PAGE alone is not a reliable diagnostic tool both in ruminants and human, it should be coupled with recent molecular techniques. Source


Mustafa Y.S.,Provincial Diagnostic Laboratory | Nazir Awan F.,Provincial Diagnostic Laboratory | Zaman T.,Provincial Diagnostic Laboratory
Buffalo Bulletin | Year: 2013

A total of 450 milk samples including both 272 buffalo and 178 cow were randomly collected in and around District Lahore to study the incidence of mastitis and antibiotic sensitivity by performing culture and sensitivity test. The prevalence of mastitis in buffalo was found to be 20.98% while that in cowwas 24.71%. The prevalences of both clinical and subclincal mastitis in buffalo were 40.35% and 59.64%, respectively, and those in cow were 61.26% and 30.63% respectively. The milk samples mixed with both mucus and blood in buffalo and cow were 5.51% and 4.49%, respectively. Quarter-wise prevalence was 47.72%, 11.36%, 36.36% and 4.54% in the left fore, left hind, right fore and right hind quarters in cow while in buffaloes, the prevalence was 0%, 68.96%, 11.49% and 19.54% in the left fore, left hind, right fore and right hind quarters, respectively. Ciprofloxacin was found highly sensitive in buffalo while gentamicin was found highly sensitive in cow. Source

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