Kandepu N.,Provimi Animal Nutrition India Pvt. Ltd. |
Kodaganur S.C.,Provimi Animal Nutrition India Pvt. Ltd. |
Mantri A.P.,Provimi Animal Nutrition India Pvt. Ltd. |
Saha S.,Provimi Animal Nutrition India Pvt. Ltd. |
Pallipadi R.M.,Provimi Animal Nutrition India Pvt. Ltd.
Eurasian Journal of Analytical Chemistry | Year: 2012
Halquinol is a mixture obtained by chlorinating 8-Hydroxy quinoline. It contains 5,7-dichloroquinolin-8-ol (57 to 74%), 5-chloroquinolin-8-ol (23 to 40%), and 7- chloroquinolin-8-ol (up to 4%). It is anantibacterial, antifungal and antiprotozoal in nature, and is used in many areas of theworld in poultryand swine rearing to fight microbial infections and in growth promotion. A simple, rapid, economic, accurate reverse phase isocratic HPLC method was developed for quantification ofHalquinol indifferent dosage forms such as suspension and bolus. The method was validated with respect tolinearity, precision, accuracy and robustness as per the International Conference on Harmonisation(ICH) guidelines. The calibration curve was linear in the range of 12-28 μg mL-1 for 5-chloroquinolin-8-ol and 33-77 μg mL-1 for 5,7-dichloroquinolin-8-ol respectively. The method has beensuccessfullyapplied for the analysis of Halquinol in pharmaceutical dosage forms. © 2012.