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Bahk Y.Y.,Yonsei University | Na B.-K.,Gyeongsang National University | Cho S.-H.,National Institute of Health | Kim J.-Y.,National Institute of Health | And 3 more authors.
Korean Journal of Parasitology | Year: 2010

Advancements in the field of proteomics have provided great opportunities for the development of diagnostic and therapeutic tools against human diseases. In this study, we analyzed haptoglobin and amyloid A protein levels of vivax malaria patients with combinations of depletion of the abundant plasma proteins, 2-dimensional gel electrophoresis (2-DE), image analysis, and mass spectrometry in the plasma between normal healthy donors and vivax malaria patients. The results showed that the expression level of haptoglobin had become significantly lower or undetectable in the plasma of vivax malaria patients due to proteolytic cleavage when compared to healthy donors on 2-DE gels. Meanwhile, serum amyloid A protein was significantly increased in vivax malaria patient's plasma with high statistical values. These 2 proteins are common acute phase reactants and further large scale evaluation with a larger number of patient's will be necessary to establish the possible clinical meaning of the existential changes of these proteins in vivax malaria patients. However, our proteomic analysis suggests the feasible values of some plasma proteins, such as haptoglobin and serum amyloid A, as associating factor candidates for vivax malaria.


Baek J.H.,Gyeongsang National University | Lee H.J.,Yonsei University | Kim Y.H.,Kansas State University | Lim K.J.,Proteometech Inc. | And 2 more authors.
Pesticide Biochemistry and Physiology | Year: 2016

The whitefly Bemisia tabaci is a very destructive pest. B. tabaci is composed of various morphologically undistinguishable biotypes, among which biotypes B and Q, in particular, draw attention because of their wide distribution in Korea and differential potentials for insecticide resistance development. To develop a biotype-specific protein marker that can readily distinguishes biotypes B from other biotypes in the field, we established an ELISA protocol based on carboxylesterase 2 (COE2), which is more abundantly expressed in biotypes B compared with Q. Recombinant COE2 was expressed, purified and used for antibody construction. Polyclonal antibodies specific to B. tabaci COE2 [anti-COE2 pAb and deglycosylated anti-COE2 pAb (DG anti-COE2 pAb)] revealed a 3-9-fold higher reactivity to biotype B COE2 than biotype Q COE2 by Western blot and ELISA analyses. DG anti-COE2 pAb exhibited low non-specific activity, demonstrating its compatibility in diagnosing biotypes. Western blot and ELISA analyses determined that one of the 11 field populations examined was biotype B and the others were biotype Q, suggesting the saturation of biotype Q in Korea. DG anti-COE2 pAb discriminates B. tabaci biotypes B and Q with high specificity and accuracy and could be useful for the development of a B. tabaci biotype diagnosis kit for on-site field applications. © 2016.


PubMed | Gyeongsang National University, Yonsei University, Seoul National University, Proteometech Inc. and Kansas State University
Type: | Journal: Pesticide biochemistry and physiology | Year: 2016

The whitefly Bemisia tabaci is a very destructive pest. B. tabaci is composed of various morphologically undistinguishable biotypes, among which biotypes B and Q, in particular, draw attention because of their wide distribution in Korea and differential potentials for insecticide resistance development. To develop a biotype-specific protein marker that can readily distinguishes biotypes B from other biotypes in the field, we established an ELISA protocol based on carboxylesterase 2 (COE2), which is more abundantly expressed in biotypes B compared with Q. Recombinant COE2 was expressed, purified and used for antibody construction. Polyclonal antibodies specific to B. tabaci COE2 [anti-COE2 pAb and deglycosylated anti-COE2 pAb (DG anti-COE2 pAb)] revealed a 3-9-fold higher reactivity to biotype B COE2 than biotype Q COE2 by Western blot and ELISA analyses. DG anti-COE2 pAb exhibited low non-specific activity, demonstrating its compatibility in diagnosing biotypes. Western blot and ELISA analyses determined that one of the 11 field populations examined was biotype B and the others were biotype Q, suggesting the saturation of biotype Q in Korea. DG anti-COE2 pAb discriminates B. tabaci biotypes B and Q with high specificity and accuracy and could be useful for the development of a B. tabaci biotype diagnosis kit for on-site field applications.


Lee T.J.,Ohio State University | Haque F.,University of Kentucky | Shu D.,University of Kentucky | Yoo J.Y.,Ohio State University | And 12 more authors.
Oncotarget | Year: 2015

Systemic siRNA administration to target and treat glioblastoma, one of the most deadly cancers, requires robust and efficient delivery platform without immunogenicity. Here we report newly emerged multivalent naked RNA nanoparticle (RNP) based on pRNA 3-way-junction (3WJ) from bacteriophage phi29 to target glioblastoma cells with folate (FA) ligand and deliver siRNA for gene silencing. Systemically injected FA-pRNA-3WJ RNPs successfully targeted and delivered siRNA into brain tumor cells in mice, and efficiently reduced luciferase reporter gene expression (4-fold lower than control). The FA-pRNA-3WJ RNP also can target human patient-derived glioblastoma stem cells, thought to be responsible for tumor initiation and deadly recurrence, without accumulation in adjacent normal brain cells, nor other major internal organs. This study provides possible application of pRNA-3WJ RNP for specific delivery of therapeutics such as siRNA, microRNA and/or chemotherapeutic drugs into glioblastoma cells without inflicting collateral damage to healthy tissues.


Kang S.,Seoul National University | Kim Y.H.,Seoul National University | Lee H.J.,Proteometech LTD | Kim B.J.,Proteometech LTD | And 2 more authors.
Journal of Asia-Pacific Entomology | Year: 2012

Sequence and length-polymorphic intron variations in the caboxylesterase 2 gene (coe2) were determined to be specific to the biotypes B and Q of Bemisia tabaci. By employing the biotype-specific coe2 intron variation as a nuclear marker, a one-step diagnostic protocol for the identification of B and Q biotypes was developed and its performance was validated for field collected B. tabaci specimens. The diagnostic results based on the coe2 intron marker were identical to those obtained from the mtCOI marker in all 256 specimens examined except for four individuals that appeared to be putative heterozygotes between B and Q biotypes. These results demonstrate a high level of accuracy of the coe2 intron marker-based protocol in distinguishing biotypes B and Q. Moreover, because the process requires only PCR amplification and gel electrophoresis, analysis of multiple samples can be done more efficiently. Based on the observation that all putative heterozygotes have the maternal background of Q biotype, they may have been created by inter-biotype cross between B type male and Q type female. If combined with the mtCOI marker, the nuclear coe2 marker would provide a better resolution than maternally inherited markers alone and facilitate the demographic study of B. tabaci biotype complex. © 2012 Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society.


Sultan M.T.,Hallym University | Li H.-M.,Hallym University | Lee Y.Z.,ProteomeTech Inc. | Lim S.S.,Hallym University | Song D.-K.,Hallym University
Korean Journal of Physiology and Pharmacology | Year: 2016

We fortuitously observed a human neutrophil intracellular free-calcium concentration ([Ca2+]i) increasing activity in the commercially available phosphodiesterase I (PDE I), which is actually dried crude venom of Crotalus atrox. As this activity was not observed with another commercially available pure PDE I, we tried to find out the causative molecule(s) present in 'crude' PDE, and identified Lys49-phospholipase A2 (Lys49-PLA2 or K49-PLA2), a catalytically inactive protein which belongs to the phospholipase A2 family, by activity-driven three HPLC (reverse phase, size exclusion, reverse phase) steps followed by SDS-PAGE and LC-MS/MS. K49-PLA2 induced Ca2+ inf ux in human neutrophils without any cytotoxic ef ect. Two calcium channel inhibitors, 2-aminoetoxydiphenyl borate (2-APB) (30 μM) and SKF-96365 (20 μM) signif cantly inhibited K49-PLA2-induced [Ca2+]i increase. These results suggest that K49-PLA2 modulates [Ca2+]i in human neutrophils via 2-APB- and SKF-96365-sensitive calcium channels without causing membrane disruption. Copyright © Korean J Physiol Pharmacol.


Lim J.I.,Yonsei University | Lee Y.-K.,Denforus Co. | Shin J.-S.,Yonsei University | Lim K.-J.,Yonsei University | Lim K.-J.,ProteomeTech Inc.
Materials Letters | Year: 2010

To improve the mechanical properties such as flexibility and bond strength of cyanoacrylates, poly(l-lactide-co-ε-caprolactone) (PLCL) copolymers were dissolved into 2 kinds of cyanoacrylates, ethyl 2-cyanoacrylate (EC) and allyl 2-cyanoacrylate (AC). Mechanical properties such as bond strength, bending-stress recovery, and crystallization intensity were measured. The optimal concentration for high bond strength was determined to be 8% PLCL (molar ratio 70:30). EC/8% PLCL (70:30) exhibited three times higher bond strength as compared with that of EC. Further, AC/8% PLCL (50:50) exhibited better bending recovery than other materials. These results indicate that cyanoacrylate/PLCL materials can be widely used as adhesives in various fields. © 2010 Elsevier B.V. All rights reserved.


Park M.J.,Yonsei University | Min J.K.,Yonsei University | Yi S.-G.,Yonsei University | Kim J.H.,Yonsei University | And 2 more authors.
Journal of Applied Physics | Year: 2015

Near-infrared photodetectors are developed using graphene/MoS2 and WSe2/MoS2 vertical heterojunctions. These heterojunctions exhibit diode-rectifying behavior in the dark and enhanced photocurrent upon near-infrared irradiation. The photocurrent increases with increasing near-infrared power, leading to the photoresponsibility of 0.14 and 0.3 A W-1 for the graphene/MoS2 and WSe2/MoS2 heterojunctions, respectively, which are much higher than the photoresponsibility reported for a multilayer MoS2 phototransistor. © 2015 AIP Publishing LLC.


Patent
Proteometech Inc. | Date: 2014-01-15

The present invention is related to a parallel line biochip for multiplex diagnosis. The parallel line biochip for multiplex diagnosis includes a plurality of line strips disposed in parallel, and a well configured to immobilize the line strips. Since the parallel line biochip for multiplex diagnosis can be used to connect two or more line strips in parallel, the parallel line biochip for multiplex diagnosis has an effect of measuring various materials present in a biological test sample at the same time.


Patent
Proteometech Inc. | Date: 2016-01-13

The present invention is related to a parallel line biochip for multiplex diagnosis. The parallel line biochip for multiplex diagnosis includes a plurality of line strips disposed in parallel, and a well configured to immobilize the line strips. Since the parallel line biochip for multiplex diagnosis can be used to connect two or more line strips in parallel, the parallel line biochip for multiplex diagnosis has an effect of measuring various materials present in a biological test sample at the same time.

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