Pronucleotein Biotechnologies Corporation

San Antonio, TX, United States

Pronucleotein Biotechnologies Corporation

San Antonio, TX, United States
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Bruno J.G.,Pronucleotein Biotechnologies Corporation | Phillips T.,Pronucleotein Biotechnologies Corporation | Montez T.,Pronucleotein Biotechnologies Corporation | Garcia A.,Pronucleotein Biotechnologies Corporation | And 3 more authors.
Journal of Fluorescence | Year: 2015

A fluorescent DNA aptamer-magnetic bead sandwich assay was developed to detect listeriolysin O (LLO) protein from pathogenic Listeria bacteria using a peroxidase-linked system, Amplex Ultra Red (AUR; derivatized resazurin) substrate, and a custom-designed handheld fluorometer. The assay is highly sensitive with demonstrated limits of detection (LODs) in the range of 4 to 61∈L. monocytogenes cells or the equivalent LLO produced by 4 to 61 cells on average in separate titration trials. Total assay processing and analysis time was approximately 30 mins. The assay has demonstrated the ability to detect 6 species of Listeria as desired by the USDA's Food Safety Inspection Service (FSIS). The portable system was designed to be used primarily with surface swab samples from fomites, but it can also be used to assess enrichment cultures. The minimal time to detect a positive enrichment culture in our hands from an initial 10 cell inoculum in 200 ml of broth has been 8 h post-incubation at 37 °C in shaker flask cultures. An optional automated magnetic bead assay processing and wash device capable of simultaneously processing 6 samples with low and consistent fluorescence background for higher volume central laboratories is also described. © 2014 Springer Science+Business Media New York.


Trademark
Pronucleotein Biotechnologies Corporation | Date: 2010-04-22

Electronic, handheld devices for detecting and quantifying target analytes, contaminants, and microorganisms in food and drink, and other substances.


Trademark
Pronucleotein Biotechnologies Corporation | Date: 2010-04-23

Electronic, handheld devices for detecting and quantifying target analytes, contaminants, and microorganisms in food and drink, and other substances.


Trademark
Pronucleotein Biotechnologies Corporation | Date: 2010-04-22

Diagnostic apparatus for testing food, namely, a device containing aptamer assays for detecting and quantifying target analytes, contaminants, and microorganisms in food and drink, and other substance.


Trademark
Pronucleotein Biotechnologies Corporation | Date: 2010-04-23

Diagnostic apparatus for testing food, namely, a device containing aptamer assays for detecting and quantifying target analytes, contaminants, and microorganisms in food and drink, and other substance.


PubMed | Pronucleotein Biotechnologies Corporation
Type: Journal Article | Journal: Journal of fluorescence | Year: 2015

A fluorescent DNA aptamer-magnetic bead sandwich assay was developed to detect listeriolysin O (LLO) protein from pathogenic Listeria bacteria using a peroxidase-linked system, Amplex Ultra Red (AUR; derivatized resazurin) substrate, and a custom-designed handheld fluorometer. The assay is highly sensitive with demonstrated limits of detection (LODs) in the range of 4 to 61L. monocytogenes cells or the equivalent LLO produced by 4 to 61 cells on average in separate titration trials. Total assay processing and analysis time was approximately 30 mins. The assay has demonstrated the ability to detect 6 species of Listeria as desired by the USDAs Food Safety Inspection Service (FSIS). The portable system was designed to be used primarily with surface swab samples from fomites, but it can also be used to assess enrichment cultures. The minimal time to detect a positive enrichment culture in our hands from an initial 10 cell inoculum in 200 ml of broth has been 8 h post-incubation at 37 C in shaker flask cultures. An optional automated magnetic bead assay processing and wash device capable of simultaneously processing 6 samples with low and consistent fluorescence background for higher volume central laboratories is also described.

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