Promis Biotech Srl

Foggia, Italy

Promis Biotech Srl

Foggia, Italy

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Russo P.,University of Foggia | Russo P.,Promis Biotech srl | Iturria I.,Food Research Unit | Mohedano M.L.,CSIC - Biological Research Center | And 6 more authors.
Applied Microbiology and Biotechnology | Year: 2015

A critical feature of probiotic microorganisms is their ability to colonize the intestine of the host. Although the microbial potential to adhere to the human gut lumen has been investigated in in vitro models, there is still much to discover about their in vivo behaviour. Zebrafish is a vertebrate model that is being widely used to investigate various biological processes shared with humans. In this work, we report on the use of the zebrafish model to investigate the in vivo colonization ability of previously characterized probiotic lactic acid bacteria. Lactobacillus plantarum Lp90, L. plantarum B2 and Lactobacillus fermentum PBCC11.5 were fluorescently tagged by transfer of the pRCR12 plasmid, which encodes the mCherry protein and which was constructed in this work. The recombinant bacteria were used to infect germ-free zebrafish larvae. After removal of bacteria, the colonization ability of the strains was monitored until 3 days post-infection by using a fluorescence stereomicroscope. The results indicated differential adhesion capabilities among the strains. Interestingly, a displacement of bacteria from the medium to the posterior intestinal tract was observed as a function of time that suggested a transient colonization by probiotics. Based on fluorescence observation, L. plantarum strains exhibited a more robust adhesion capability. In conclusion, the use of pRCR12 plasmid for labelling Lactobacillus strains provides a powerful and very efficient tool to monitor the in vivo colonization in zebrafish larvae and to investigate the adhesion ability of probiotic microorganisms. © 2015, Springer-Verlag Berlin Heidelberg.


Russo P.,University of Foggia | Russo P.,Promis Biotech srl | de Chiara M.L.V.,University of Foggia | Capozzi V.,University of Foggia | And 7 more authors.
LWT - Food Science and Technology | Year: 2016

Fermented oat-based foods offer attractive prospects within the market of non-dairy functional products, since they are suitable substrates for the delivery of probiotic microorganisms, and are significant sources of dietary fiber, both insoluble and soluble such as β-glucan, good quality fat and other phytochemicals important for human health.In the present work, whole oat flour was fermented with probiotic Lactobacillus plantarum strains to produce new functional foods with improved nutritional and technological features. Viability of the probiotic and the main technological, physico-chemical, nutritional and sensorial parameters were monitored at 7, 14 and 21 days of cold storage. The microbial survival was higher than 5x108 cfu g-1 at the end of the shelf life. After the fermentation step, viscosity was higher in products inoculated with the exopolysaccharide-producing L. plantarum strain Lp90. However, a subsequent viscosity reduction was detected in all the samples throughout the storage period, consistent with the observed concentration decrease of the oat β-glucan. Vitamin B2 content was about two-fold higher in products fermented by the riboflavin-overproducing LpB2, and in these samples the riboflavin concentration further increased during cold storage. © 2015 Elsevier Ltd.


Capozzi V.,University of Foggia | Capozzi V.,Promis Biotech Srl | Russo P.,University of Foggia | Russo P.,Promis Biotech Srl | And 3 more authors.
Applied Microbiology and Biotechnology | Year: 2012

Wheat contains various essential nutrients including the B group of vitamins. However, B group vitamins, normally present in cereals-derived products, are easily removed or destroyed during milling, food processing or cooking. Lactic acid bacteria (LAB) are widely used as starter cultures for the fermentation of a large variety of foods and can improve the safety, shelf life, nutritional value, flavor and overall quality of the fermented products. In this regard, the identification and application of strains delivering health-promoting compounds is a fascinating field. Besides their key role in food fermentations, several LAB found in the gastrointestinal tract of humans and animals are commercially used as probiotics and possess generally recognized as safe status. LAB are usually auxotrophic for several vitamins although certain strains of LAB have the capability to synthesize water-soluble vitamins such as those included in the B group. In recent years, a number of biotechnological processes have been explored to perform a more economical and sustainable vitamin production than that obtained via chemical synthesis. This review article will briefly report the current knowledge on lactic acid bacteria synthesis of vitamins B2, B11 and B12 and the potential strategies to increase B-group vitamin content in cereals-based products, where vitamins-producing LAB have been leading to the elaboration of novel fermented functional foods. In addition, the use of genetic strategies to increase vitamin production or to create novel vitaminproducing strains will be also discussed. © Springer-Verlag Berlin Heidelberg 2012.


Russo P.,University of Foggia | Russo P.,Promis Biotech Srl | Capozzi V.,University of Foggia | Capozzi V.,Promis Biotech Srl | And 6 more authors.
Applied Microbiology and Biotechnology | Year: 2014

Lactobacillus fermentum isolated from sourdough was able to produce riboflavin. Spontaneous roseoflavin-resistant mutants were obtained by exposing the wild strain (named L. fermentum PBCC11) to increasing concentrations of roseoflavin. Fifteen spontaneous roseoflavin-resistant mutants were isolated, and the level of vitamin B2 was quantified by HPLC. Seven mutant strains produced concentrations of vitamin B2 higher than 1 mg L -1. Interestingly, three mutants were unable to overproduce riboflavin even though they were able to withstand the selective pressure of roseoflavin. Alignment of the rib leader region of PBCC11 and its derivatives showed only point mutations at two neighboring locations of the RFN element. In particular, the highest riboflavin-producing isolates possess an A to G mutation at position 240, while the lowest riboflavin producer carries a T to A substitution at position 236. No mutations were detected in the derivative strains that did not have an overproducing phenotype. The best riboflavin overproducing strain, named L. fermentum PBCC11.5, and its parental strain were used to fortify bread. The effect of two different periods of fermentation on the riboflavin level was compared. Bread produced using the coinoculum yeast and L. fermentum PBCC11.5 led to an approximately twofold increase of final vitamin B2 content. © 2014 Springer-Verlag Berlin Heidelberg.


Berbegal C.,University of Foggia | Berbegal C.,University of Valencia | Pena N.,University of Foggia | Russo P.,University of Foggia | And 7 more authors.
Food Microbiology | Year: 2016

Malolactic fermentation (MLF) is a secondary fermentation in wine that usually takes place during or at the end of alcoholic fermentation. Lactobacillus plantarum is able to conduct MLF (particularly under high pH conditions and in co-inoculation with yeasts), and some strains are commercially used as MLF starter cultures. Recent evidences suggest a further use of selected L. plantarum strains for the pre-alcoholic acidification of grape must. In this study, we have carried out an integrated (molecular, technological, and biotechnological) characterization of L. plantarum strains isolated from Apulian wines in order to combine the two protechnological features (MLF performances and must acidification aptitudes). Several parameters such as sugar, pH and ethanol tolerance, resistance to lyophilisation and behaviour in grape must were evaluated. Moreover, the expression of stress gene markers was investigated and was linked to the ability of L. plantarum strains to grow and perform MLF. Co-inoculation of Saccharomyces cerevisiae and L. plantarum in grape must improves the bacterial adaptation to harsh conditions of wine and reduced total fermentation time. For the first time, we applied a polyphasic approach for the characterization of L. plantarum in reason of the MLF performances. The proposed procedure can be generalized as a standard method for the selection of bacterial resources for the design of MLF starter cultures tailored for high pH must. © 2016 Elsevier Ltd.


Arena M.P.,University of Foggia | Russo P.,University of Foggia | Russo P.,Promis Biotech srl | Capozzi V.,University of Foggia | And 5 more authors.
Journal of Functional Foods | Year: 2016

The influence of oat and barley β-glucans on some features of diverse probiotic strains was investigated. Neither barley nor oat β-glucans supported the growth of any of the investigated strains when used as the sole carbon source in fermentation media. No protective effect by β-glucans was observed on bacterial survival to in vitro simulation of the human digestive tract. Moreover, the presence of β-glucans did not enhance the ability of probiotic bacteria to adhere on human intestinal cells. The immunomodulatory activities of probiotics, β-glucans, and their combinations were studied and compared in vitro by transcriptional analysis of immune-related genes on LPS-stimulated THP-1 macrophages. Immune modulating properties were evidenced. In particular, mixtures of probiotic microorganisms and barley β-glucans exhibited synergistic effects in modulating the transcriptional level of several immune-related genes, leading to an overall enhanced anti-inflammatory effect. Together, these findings suggest a promising application of probiotic bacteria and β-glucans in the preparation of dietary mixtures presenting health-promoting features such as immunomodulatory properties. © 2016 Elsevier Ltd.


Russo P.,University of Foggia | Russo P.,Promis Biotech S.r.l. | De Chiara M.L.V.,University of Foggia | Vernile A.,University of Foggia | And 6 more authors.
BioMed Research International | Year: 2014

Due to the increasing interest for healthy foods, the feasibility of using fresh-cut fruits to vehicle probiotic microorganisms is arising scientific interest. With this aim, the survival of probiotic lactic acid bacteria, belonging to Lactobacillus plantarum and Lactobacillus fermentum species, was monitored on artificially inoculated pineapple pieces throughout storage. The main nutritional, physicochemical, and sensorial parameters of minimally processed pineapples were monitored. Finally, probiotic Lactobacillus were further investigated for their antagonistic effect against Listeria monocytogenes and Escherichia coli O157:H7 on pineapple plugs. Our results show that at eight days of storage, the concentration of L. plantarum and L. fermentum on pineapples pieces ranged between 7.3 and 6.3 log cfu g -1, respectively, without affecting the final quality of the fresh-cut pineapple. The antagonistic assays indicated that L. plantarum was able to inhibit the growth of both pathogens, while L. fermentum was effective only against L. monocytogenes. This study suggests that both L. plantarum and L. fermentum could be successfully applied during processing of fresh-cut pineapples, contributing at the same time to inducing a protective effect against relevant foodborne pathogens. © 2014 Pasquale Russo et al.


Arena M.P.,University of Foggia | Russo P.,University of Foggia | Russo P.,Promis Biotech Srl | Capozzi V.,University of Foggia | And 4 more authors.
Applied Microbiology and Biotechnology | Year: 2014

The probiotic potential of Lactobacillus plantarum and Lactobacillus fermentum strains, capable of overproducing riboflavin, was investigated. The riboflavin production was quantified in co-cultures of lactobacilli and human intestinal epithelial cells, and the riboflavin overproduction ability was confirmed. When milk and yogurt were used as carrier matrices, L. plantarum and L. fermentum strains displayed a significant ability to survive through simulated gastrointestinal transit. Adhesion was studied on both biotic and abiotic surfaces. Both strains adhered strongly on Caco-2 cells, negatively influenced the adhesion of Escherichia coli O157:H7, and strongly inhibited the growth of three reference pathogenic microbial strains. Resistance to major antibiotics and potential hemolytic activity were assayed. Overall, this study reveals that these Lactobacillus stains are endowed with promising probiotic properties and thus are candidates for the development of novel functional food which would be both enriched in riboflavin and induce additional health benefits, including a potential in situ riboflavin production, once the microorganisms colonize the host intestine. © 2014 Springer-Verlag.


Russo P.,University of Foggia | Russo P.,Promis Biotech srl | Pena N.,University of Foggia | de Chiara M.L.V.,University of Foggia | And 3 more authors.
Food Research International | Year: 2015

Minimally processed fruits are an ideal alternative to dairy products to deliver probiotic microorganisms. At the same time, several innovative employments of lactic acid bacteria (LAB) have been proposed in the food industry, including bio-fortification with nutritional compounds and bio-protection against foodborne pathogenic bacteria. In this study, probiotic riboflavin over-producing Lactobacillus plantarum B2 and Lactobacillus fermentum PBCC11.5 were inoculated on fresh-cut cantaloupe by immersion in a dipping solution. The viability of probiotic microorganisms and the main physico-chemical parameters of melon pieces, including the riboflavin content, were monitored for 11. days of storage under refrigerated conditions. Finally, both probiotics were tested for their antagonistic effect against different concentrations of an isolate of Listeria monocytogenes from fruit origin. Overall, high viability of both probiotics species was found at the end of the shelf life. The main technological and nutritional parameters of the fruits were unaffected by probiotic-enrichment, except some sensorial attributes when melons were inoculated with L. plantarum B2. The riboflavin content increased about two-fold in probiotic cantaloupe. Moreover, L. plantarum B2 and L. fermentum PBCC11.5 showed a good ability to reduce the level of L. monocytogenes on artificially contaminated melons. In conclusion, the results of this work encourage further implementation of new foods with multifunctional properties. © 2015 Elsevier Ltd.

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