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Napoli, Italy

Arena M.P.,University of Foggia | Russo P.,University of Foggia | Russo P.,Promis Biotech Srl | Capozzi V.,University of Foggia | And 5 more authors.
Journal of Functional Foods | Year: 2016

The influence of oat and barley β-glucans on some features of diverse probiotic strains was investigated. Neither barley nor oat β-glucans supported the growth of any of the investigated strains when used as the sole carbon source in fermentation media. No protective effect by β-glucans was observed on bacterial survival to in vitro simulation of the human digestive tract. Moreover, the presence of β-glucans did not enhance the ability of probiotic bacteria to adhere on human intestinal cells. The immunomodulatory activities of probiotics, β-glucans, and their combinations were studied and compared in vitro by transcriptional analysis of immune-related genes on LPS-stimulated THP-1 macrophages. Immune modulating properties were evidenced. In particular, mixtures of probiotic microorganisms and barley β-glucans exhibited synergistic effects in modulating the transcriptional level of several immune-related genes, leading to an overall enhanced anti-inflammatory effect. Together, these findings suggest a promising application of probiotic bacteria and β-glucans in the preparation of dietary mixtures presenting health-promoting features such as immunomodulatory properties. © 2016 Elsevier Ltd.

Russo P.,University of Foggia | Russo P.,Promis Biotech Srl | Pena N.,University of Foggia | de Chiara M.L.V.,University of Foggia | And 3 more authors.
Food Research International | Year: 2015

Minimally processed fruits are an ideal alternative to dairy products to deliver probiotic microorganisms. At the same time, several innovative employments of lactic acid bacteria (LAB) have been proposed in the food industry, including bio-fortification with nutritional compounds and bio-protection against foodborne pathogenic bacteria. In this study, probiotic riboflavin over-producing Lactobacillus plantarum B2 and Lactobacillus fermentum PBCC11.5 were inoculated on fresh-cut cantaloupe by immersion in a dipping solution. The viability of probiotic microorganisms and the main physico-chemical parameters of melon pieces, including the riboflavin content, were monitored for 11. days of storage under refrigerated conditions. Finally, both probiotics were tested for their antagonistic effect against different concentrations of an isolate of Listeria monocytogenes from fruit origin. Overall, high viability of both probiotics species was found at the end of the shelf life. The main technological and nutritional parameters of the fruits were unaffected by probiotic-enrichment, except some sensorial attributes when melons were inoculated with L. plantarum B2. The riboflavin content increased about two-fold in probiotic cantaloupe. Moreover, L. plantarum B2 and L. fermentum PBCC11.5 showed a good ability to reduce the level of L. monocytogenes on artificially contaminated melons. In conclusion, the results of this work encourage further implementation of new foods with multifunctional properties. © 2015 Elsevier Ltd.

Arena M.P.,University of Foggia | Russo P.,University of Foggia | Russo P.,Promis Biotech Srl | Capozzi V.,University of Foggia | And 4 more authors.
Applied Microbiology and Biotechnology | Year: 2014

The probiotic potential of Lactobacillus plantarum and Lactobacillus fermentum strains, capable of overproducing riboflavin, was investigated. The riboflavin production was quantified in co-cultures of lactobacilli and human intestinal epithelial cells, and the riboflavin overproduction ability was confirmed. When milk and yogurt were used as carrier matrices, L. plantarum and L. fermentum strains displayed a significant ability to survive through simulated gastrointestinal transit. Adhesion was studied on both biotic and abiotic surfaces. Both strains adhered strongly on Caco-2 cells, negatively influenced the adhesion of Escherichia coli O157:H7, and strongly inhibited the growth of three reference pathogenic microbial strains. Resistance to major antibiotics and potential hemolytic activity were assayed. Overall, this study reveals that these Lactobacillus stains are endowed with promising probiotic properties and thus are candidates for the development of novel functional food which would be both enriched in riboflavin and induce additional health benefits, including a potential in situ riboflavin production, once the microorganisms colonize the host intestine. © 2014 Springer-Verlag.

Capozzi V.,University of Foggia | Capozzi V.,Promis Biotech Srl | Russo P.,University of Foggia | Russo P.,Promis Biotech Srl | And 3 more authors.
Applied Microbiology and Biotechnology | Year: 2012

Wheat contains various essential nutrients including the B group of vitamins. However, B group vitamins, normally present in cereals-derived products, are easily removed or destroyed during milling, food processing or cooking. Lactic acid bacteria (LAB) are widely used as starter cultures for the fermentation of a large variety of foods and can improve the safety, shelf life, nutritional value, flavor and overall quality of the fermented products. In this regard, the identification and application of strains delivering health-promoting compounds is a fascinating field. Besides their key role in food fermentations, several LAB found in the gastrointestinal tract of humans and animals are commercially used as probiotics and possess generally recognized as safe status. LAB are usually auxotrophic for several vitamins although certain strains of LAB have the capability to synthesize water-soluble vitamins such as those included in the B group. In recent years, a number of biotechnological processes have been explored to perform a more economical and sustainable vitamin production than that obtained via chemical synthesis. This review article will briefly report the current knowledge on lactic acid bacteria synthesis of vitamins B2, B11 and B12 and the potential strategies to increase B-group vitamin content in cereals-based products, where vitamins-producing LAB have been leading to the elaboration of novel fermented functional foods. In addition, the use of genetic strategies to increase vitamin production or to create novel vitaminproducing strains will be also discussed. © Springer-Verlag Berlin Heidelberg 2012.

Russo P.,University of Foggia | Russo P.,Promis Biotech Srl | de Chiara M.L.V.,University of Foggia | Capozzi V.,University of Foggia | And 7 more authors.
LWT - Food Science and Technology | Year: 2016

Fermented oat-based foods offer attractive prospects within the market of non-dairy functional products, since they are suitable substrates for the delivery of probiotic microorganisms, and are significant sources of dietary fiber, both insoluble and soluble such as β-glucan, good quality fat and other phytochemicals important for human health.In the present work, whole oat flour was fermented with probiotic Lactobacillus plantarum strains to produce new functional foods with improved nutritional and technological features. Viability of the probiotic and the main technological, physico-chemical, nutritional and sensorial parameters were monitored at 7, 14 and 21 days of cold storage. The microbial survival was higher than 5x108 cfu g-1 at the end of the shelf life. After the fermentation step, viscosity was higher in products inoculated with the exopolysaccharide-producing L. plantarum strain Lp90. However, a subsequent viscosity reduction was detected in all the samples throughout the storage period, consistent with the observed concentration decrease of the oat β-glucan. Vitamin B2 content was about two-fold higher in products fermented by the riboflavin-overproducing LpB2, and in these samples the riboflavin concentration further increased during cold storage. © 2015 Elsevier Ltd.

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