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PubMed | Indian Veterinary Research Institute, Project Directorate on Cattle ICAR and Veterinary College and Research Institute Campus
Type: Journal Article | Journal: Acta veterinaria Hungarica | Year: 2015

Avian nephritis virus (ANV) infects poultry flocks worldwide, but no confirmed cases have been reported from India so far. In the current study, disease investigation was carried out in 21 broiler flocks at different parts of India with clinical signs of nephritis, uneven and stunted growth, diarrhoea, reduced body weight, and mortality up to 9.72%. Out of the 21 flocks screened, two were found positive for ANV in RT-PCR assay. BLAST analysis revealed that the ANV of Indian origin was closely related to ANV-1 strains reported from Japan, Hungary and China. However, comparison of a small portion (~12% of nucleotides, i.e. ~60 nts, common site for ANV-1 and ANV-3, position 2200-2260 of ORF 1a gene) of the Indian ANV sequence with ANV-3 sequences revealed 89-93% identities with different ANV-3 isolates. Phylogenetically, ANV-1 forms three clades, and the Indian ANV clustered under clade II. This study confirms the existence of ANV in Indian poultry flocks and is the first report on the molecular detection and genetic characterisation of ANV from India.

Deb R.,Project Directorate on Cattle ICAR | Singh U.,Project Directorate on Cattle ICAR | Kumar S.,Project Directorate on Cattle ICAR | Kumar A.,Project Directorate on Cattle ICAR | And 3 more authors.
Indian Journal of Animal Sciences | Year: 2013

Toll like receptor 4 (TLR4) is implicated as a receptor mediating cellular activation in response to bacterial lipopolysaccharides (LPS) of gram negative organisms which is the principal pathogen associated molecular patterns (PAMPs) of TLR 4. Thirty-one single nucleotide polymorphisms (SNP) were detected in bovine TLR4 gene. The SNP (g.9788C>T) found in the Toll IL-1 receptor (TIR) domain of the bovine TLR4 is reported to be associated with mastitis in earlier studies. In the present study we have amplified the TIR domain containing the above SNP site from Frieswal (Holstein Friesian × Sahiwal) crossbreed cattle blood genomic DNA. The amplified products of 126 unrelated Frieswal TIR domain of TLR4 gene were digested with Hinf I restriction enzyme and genotyping was analyzed. Our studies revealed that CC alleles (0.97) are more prevalent than TT alleles (0.03) in Frieswal genome.

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