Programa Regional del Noroeste para el Doctorado en Biotecnologia

Culiacán, Mexico

Programa Regional del Noroeste para el Doctorado en Biotecnologia

Culiacán, Mexico

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Montoya-Rodriguez A.,Programa Regional del Noroeste para el Doctorado en Biotecnologia | Montoya-Rodriguez A.,University of Illinois at Urbana - Champaign | Milan-Carrillo J.,Programa Regional del Noroeste para el Doctorado en Biotecnologia | Dia V.P.,University of Illinois at Urbana - Champaign | And 2 more authors.
Proteome Science | Year: 2014

Background: Atherosclerosis is considered a progressive disease that affects arteries that bring blood to the heart, to the brain and to the lower end. It derives from endothelial dysfunction and inflammation, which play an important role in the thrombotic complications of atherosclerosis. Cardiovascular disease is the leading cause of death around the world and one factor that can contribute to its progression and prevention is diet. Our previous study found that amaranth hydrolysates inhibited LPS-induced inflammation in human and mouse macrophages by preventing activation of NF-κB signaling. Furthermore, extrusion improved the anti-inflammatory effect of amaranth protein hydrolysates in both cell lines, probably attributed to the production of bioactive peptides during processing. Therefore, the objective of this study was to compare the anti-atherosclerotic potential of pepsin-pancreatin hydrolysates from unprocessed and extruded amaranth in THP-1 lipopolysaccharide-induced human macrophages and suggest the mechanism of action.Results: Unprocessed amaranth hydrolysate (UAH) and extruded amaranth hydrolysate (EAH) showed a significant reduction in the expression of interleukin-4 (IL-4) (69% and 100%, respectively), interleukin-6 (IL-6) (64% and 52%, respectively), interleukin-22 (IL-22) (55% and 70%, respectively). Likewise, UAH and EAH showed a reduction in the expression of monocyte-chemo attractant protein-1 (MCP-1) (35% and 42%, respectively), transferrin receptor-1 (TfR-1) (48% and 61%, respectively), granulocyte-macrophage colony-stimulating factor (GM-CSF) (59% and 63%, respectively), and tumor necrosis factor-α (TNF-α) (60% and 63%, respectively). Also, EAH reduced the expression of lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) (27%), intracellular adhesion molecule-1 (ICAM-1) (28%) and matrix metalloproteinase-9 (MMP-9) (19%), important molecular markers in the atherosclerosis pathway. EAH, led to a reduction of 58, 52 and 79% for LOX-1, ICAM-1 and MMP-9, respectively, by confocal microscopy.Conclusions: Extruded amaranth hydrolysate showed potential anti-atherosclerotic effect in LPS-induced THP-1 human macrophage-like cells by reducing the expression of proteins associated with LOX-1 signaling pathway. © 2014 Montoya-Rodríguez et al.; licensee BioMed Central Ltd.


Montoya-Rodriguez A.,Programa Regional del Noroeste para el Doctorado en Biotecnologia | Montoya-Rodriguez A.,University of Illinois at Urbana - Champaign | de Mejia E.G.,University of Illinois at Urbana - Champaign
Food Research International | Year: 2015

The objectives were to evaluate and to compare the effect of pure peptides from amaranth proteins on markers that promote atherosclerosis, in vitro. Amaranth pure peptides with potential anti-atherosclerotic effect, HGSEPFGPR, RPRYPWRYT and RDGPFPWPWYSH, were studied using lipopolysaccharide-induced THP-1 human macrophage-like cells. Lectin-like oxidized low-density lipoprotein receptor 1 (LOX-1) expression was reduced by peptides HGSEPFGPR (83%), RDGPFPWPWYSH (49%) and RPRYPWRYT (63%); while intracellular adhesion molecule-1 (ICAM-1) was reduced by peptides RDGPFPWPWYSH (27%) and RPRYPWRYT (39%); and matrix metalloproteinase-9 (MMP-9) expression was reduced by 52% (HGSEPFGPR), 41% (RDGPFPWPWYSH) and 29% (RPRYPWRYT). Using confocal microscopy, peptide HGSEPFGPR reduced LOX-1, ICAM-1 and MMP-9 expression by 74%, 59% and 80%, respectively. RPRYPWRYT reduced LOX-1, ICAM-1 and MMP-9 expression by 48%, 49% and 71%, respectively. RPRYPWRYT reduced MCP-1 and TGF-β cellular protein expression (74% and 78%, respectively); 89% (IL-6 and IL-1α); 83% (IFN-γ); 87% and 58% (TNF-α and TNF-β). HGSEPFGPR showed significant reduction of 27%, 31%, 41%, 57%, and 61% for GRO-α, MCP-1, IL-6, IL-1α, and RANTES, respectively. HGSEPFGPR showed better interactions with LOX-1 crystal structure than RPRYPWRYT. Amaranth pure peptides reduced the expression of proteins associated with LOX-1 signaling pathway, in vitro. © 2015 Elsevier Ltd.


Montoya-Rodriguez A.,Programa Regional del Noroeste para el Doctorado en Biotecnologia | Montoya-Rodriguez A.,University of Illinois at Urbana - Champaign | de Mejia E.G.,University of Illinois at Urbana - Champaign | Dia V.P.,University of Illinois at Urbana - Champaign | And 2 more authors.
Molecular Nutrition and Food Research | Year: 2014

Scope: The objective was to compare the anti-inflammatory potential of unprocessed and extruded amaranth pepsin/pancreatin hydrolysates in LPS-induced human THP-1 macrophages-like and mouse RAW 264.7 macrophages focusing on their anti-inflammatory mechanism of action related to NF-κB signaling pathway. Methods and results: Amaranth hydrolysates were characterized by MS-MS and tested for anti-inflammatory effects on human and mouse macrophages. Peptides found in extruded amaranth hydrolysates displayed antioxidant capacity, angiotensin converting enzyme-inhibitor activity, and dipeptidyl peptidase-IV inhibitor activity. Gly-Pro-Arg peptide was present and reported as antithrombotic. Extruded amaranth hydrolysates (1 mg/mL) significantly reduced tumor necrosis factor alpha secretion in THP-1 and RAW 264.7 cells by 36.5 and 33.5%, respectively; with concomitant reduction in PGE2 (15.4 and 31.4%), and COX-2 (38.1 and 67.6%), respectively. Phosphorylation of IKK-α was significantly reduced by 52.5 and 88.2% leading to reduced phosphorylation of IκB-α (86.1 and 66.2%), respectively; resulting in a reduction in the expression of p65 NF-κB subunits in the nucleus by 64.2% for THP-1 and 70.7% for RAW 264.7 cells. Conclusion: Amaranth hydrolysates inhibited LPS-induced inflammation in human and mouse macrophages by preventing activation of NF-κB signaling. Extrusion improved anti-inflammatory effect of amaranth hydrolysates in both cells, which might be attributed to the production of bioactive peptides during processing. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Reyes-Moreno C.,Autonomous University of Sinaloa | Arguelles-Lopez O.D.,Autonomous University of Sinaloa | Rochin-Medina J.J.,Autonomous University of Sinaloa | Milan-Carrillo J.,Autonomous University of Sinaloa | And 5 more authors.
Plant Foods for Human Nutrition | Year: 2012

The objective of this study was to determine the best combination of extrusion process variables for the production of whole quality protein maize (EQPMF) and common bean (ECBF) flours to prepare a high antioxidant activity mixture (EQPMF + ECBF) suitable to produce a nutraceutical beverage with high acceptability elaborated with a traditional Mexican formulation. Processing conditions were obtained from a factorial combination of barrel temperature (BT = 120-170 °C) and screw speed (SS = 120-200 rpm). Response surface methodology was applied to obtain maximum values for antioxidant activity (AoxA) of the flour mixture (EQPMF + ECBF) and acceptability (A) of the nutraceutical beverage. The best combinations of extrusion process variables for EQPMF and ECBF to prepare an optimized mixture (60%EQPMF + 40%ECBF) were BT = 98 °C/SS = 218 rpm and BT = 105 °C/SS = 83 rpm, respectively. The optimized mixture had AoxA = 14,320 μmol Trolox equivalent (TE)/100 g sample dry weight (dw) and a calculated protein efficiency ratio (C-PER) of 2. 17. A 200 ml portion of a beverage prepared with 25 g of the optimized flour mixture had AoxA = 3,222 μmol TE, and A = 89 (level of satisfaction "I like it extremely"). This nutraceutical beverage could be used as an alternative to beverages with low nutritional/nutraceutical value, such as those prepared with water, simple sugars, artificial flavoring and colorants, which are widely offered in the market. © 2012 Springer Science+Business Media New York.


Heiras-Palazuelos M.J.,Autonomous University of Sinaloa | Ochoa-Lugo M.I.,Autonomous University of Sinaloa | Gutierrez-Dorado R.,Autonomous University of Sinaloa | Lopez-Valenzuela J.A.,Autonomous University of Sinaloa | And 4 more authors.
International Journal of Food Sciences and Nutrition | Year: 2013

Chickpeas are rich sources of highly nutritious protein and dietary fibre; the health benefits of consuming legumes such as antioxidant activity (AoxA) could be effective for the expansion of their food uses. The technological properties and antioxidant potential of five pigmented chickpea cultivars were evaluated. Protein content of the grains varied from 24.9 to 27.4 g/100 g sample (dw). The cooking time (CT) of the whole grains ranged from 90.5 to 218.5 min; the lowest CT corresponded to Black ICC3761 cultivar. The total phenolic content (TPC) and AoxA [oxygen radical absorbance capacity (ORAC) value] varied from 1.23 to 1.51 mg GAE/g sample (dw) and from 5011 to 5756 μmol TE/100 g sample (dw), respectively; Red ICC13124 showed the highest ORAC value. The differences in technological properties and AoxA among cultivars could be used in chickpea breeding programmes. Chickpea cultivars could contribute significantly to the management and/or prevention of degenerative diseases associated with free radical damage. © 2012 Informa UK, Ltd.

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