Time filter

Source Type

Spindler C.,Federal University of Rio Grande do Sul | Cechinel L.R.,Federal University of Rio Grande do Sul | Basso C.,Federal University of Rio Grande do Sul | Moyses F.,Federal University of Rio Grande do Sul | And 7 more authors.
Cellular and Molecular Neurobiology | Year: 2014

Studies have pointed out the relationship between neuroprotective exercise effects and epigenetic mechanisms on the hippocampus. Considering the role of frontal cortex on brain functions, we investigated the impact of different exercise protocols on enzymatic system involved with histone acetylation status, histone acetyltransferases (HATs), and histone desacetylases (HDACs) in frontal cortices from Wistar rats. Male Wistar rats aged 3 months were submitted to a single session or a daily running protocol during 2 weeks. The single session enhanced HAT activity, while the moderate daily exercise protocol reduced the HDAC activity. Our results indicate that frontal cortex is susceptible to epigenetic modulation following exercise and that both exercise protocols seem to induce a histone hyperacetylation condition in this brain area. © 2014 Springer Science+Business Media New York.


de Moura A.C.,Federal University of Health Sciences, Porto Alegre | da Silva I.R.V.,Programa Of Pos Graduacao Em Biociencias abilitacao Do Centro Universitario Metodista Do Ipa | Reinaldo G.,Curso Of Fisioterapia Do Centro Universitario Metodista Do Ipa | Dani C.,Programa Of Pos Graduacao Em Biociencias abilitacao Do Centro Universitario Metodista Do Ipa | And 2 more authors.
Cellular and Molecular Neurobiology | Year: 2015

In rats, variations in the levels of neuromodulatory molecules and in the expression of their receptors are observed during pregnancy and postpartum. These changes may contribute to the development and management of maternal behavior. The frequency of licking the pups is used to evaluate maternal care, having mothers with low licking (LL) and high licking (HL) frequencies. Previously, we found that HL had increased levels of transcriptional expression of the receptors for serotonin (HTR1a, HTR1b), estrogen (Erα), dopamine (D1a), and prolactin (Prlr) than LL in the olfactory bulb (OB); however, the molecular mechanisms behind this phenomenon are unknown. Since evidences pointed out that epigenetic marks, which may alter gene expression, are modulated by environmental factors such as exercise, diet, maternal care, and xenobiotic exposure, our objective was to verify the acetylation levels of histone-H4 in the OB of LL and HL rats. Maternal behavior was studied for the first 7 postpartum days. LL (n = 4) and HL (n = 5) mothers were selected according to the behavior of licking their pups. Acetylation levels of histone-H4 were determined using the Global Histone-H4 Acetylation Assay Kit and expressed as ng/mg protein (mean ± SD). Analysis revealed that HL (278.36 ± 68.95) had increased H4 acetylation levels than LL (183.24 ± 73.05; p = 0.045). The enhanced expression of the previously studied receptors in the OB could be related, at least in part, to the hyperacetylation status of histone-H4 here observed. Afterward, the modulation of histone acetylation levels could exert a pivotal role through molecular mechanisms involved in the different patterns of maternal behavior. © 2015 Springer Science+Business Media New York


PubMed | Federal University of Health Sciences, Porto Alegre, Programa Of Pos Graduacao Em Biociencias abilitacao Do Centro Universitario Metodista Do Ipa and Curso Of Fisioterapia Do Centro Universitario Metodista Do Ipa
Type: Journal Article | Journal: Cellular and molecular neurobiology | Year: 2016

In rats, variations in the levels of neuromodulatory molecules and in the expression of their receptors are observed during pregnancy and postpartum. These changes may contribute to the development and management of maternal behavior. The frequency of licking the pups is used to evaluate maternal care, having mothers with low licking (LL) and high licking (HL) frequencies. Previously, we found that HL had increased levels of transcriptional expression of the receptors for serotonin (HTR1a, HTR1b), estrogen (Er), dopamine (D1a), and prolactin (Prlr) than LL in the olfactory bulb (OB); however, the molecular mechanisms behind this phenomenon are unknown. Since evidences pointed out that epigenetic marks, which may alter gene expression, are modulated by environmental factors such as exercise, diet, maternal care, and xenobiotic exposure, our objective was to verify the acetylation levels of histone-H4 in the OB of LL and HL rats. Maternal behavior was studied for the first 7 postpartum days. LL (n=4) and HL (n=5) mothers were selected according to the behavior of licking their pups. Acetylation levels of histone-H4 were determined using the Global Histone-H4 Acetylation Assay Kit and expressed as ng/mg protein (meanSD). Analysis revealed that HL (278.3668.95) had increased H4 acetylation levels than LL (183.2473.05; p=0.045). The enhanced expression of the previously studied receptors in the OB could be related, at least in part, to the hyperacetylation status of histone-H4 here observed. Afterward, the modulation of histone acetylation levels could exert a pivotal role through molecular mechanisms involved in the different patterns of maternal behavior.


da Silveira F.P.,Programa Of Pos Graduacao Em Biociencias abilitacao Do Centro Universitario Metodista Do Ipa | Basso C.,Federal University of Rio Grande do Sul | Raupp W.,Federal University of Rio Grande do Sul | Dalpiaz M.,Programa Of Pos Graduacao Em Biociencias abilitacao Do Centro Universitario Metodista Do Ipa | And 5 more authors.
Journal of Physiological Sciences | Year: 2016

Our aim was to compare the basal levels of plasma brain-derived neurotrophic factor (BDNF) and global histone H4 acetylation in peripheral blood mononuclear cells (PBMCs) of healthy amateur runners (EXE group) with sedentary individuals (SED group) as well as to investigate the acute effect of a running race on these markers in the EXE group. Five days before the race, all participants were submitted to a basal blood collection. On the race day, two blood samples were collected in the EXE group before the running started and immediately at the end. In the basal period, a significant increase of plasma BDNF levels in the EXE individuals when compared to the SED group (p = 0.036) was demonstrated, while no difference in global histone H4 acetylation levels was observed. These parameters were unaltered in the EXE group after the race. The increased levels of BDNF might be linked to healthy middle-aged runners’ phenotype. © 2016 The Physiological Society of Japan and Springer Japan


PubMed | Federal University of Health Sciences, Porto Alegre, Programa Of Pos Graduacao Em Biociencias abilitacao Do Centro Universitario Metodista Do Ipa and Federal University of Rio Grande do Sul
Type: | Journal: The journal of physiological sciences : JPS | Year: 2016

Our aim was to compare the basal levels of plasma brain-derived neurotrophic factor (BDNF) and global histone H4 acetylation in peripheral blood mononuclear cells (PBMCs) of healthy amateur runners (EXE group) with sedentary individuals (SED group) as well as to investigate the acute effect of a running race on these markers in the EXE group. Five days before the race, all participants were submitted to a basal blood collection. On the race day, two blood samples were collected in the EXE group before the running started and immediately at the end. In the basal period, a significant increase of plasma BDNF levels in the EXE individuals when compared to the SED group (p=0.036) was demonstrated, while no difference in global histone H4 acetylation levels was observed. These parameters were unaltered in the EXE group after the race. The increased levels of BDNF might be linked to healthy middle-aged runners phenotype.

Loading Programa Of Pos Graduacao Em Biociencias abilitacao Do Centro Universitario Metodista Do Ipa collaborators
Loading Programa Of Pos Graduacao Em Biociencias abilitacao Do Centro Universitario Metodista Do Ipa collaborators