Rull J.,Institute Ecologia |
Birke A.,Institute Ecologia |
Ortega R.,Institute Ecologia |
Montoya P.,Programa Moscamed Moscafrut |
Lopez L.,Programa Moscamed Moscafrut
Entomologia Experimentalis et Applicata | Year: 2012
The sterile insect technique (SIT) requires production of large quantities of sterile males able to successfully compete with wild males for wild females. During eradication of a pest population, the release of fertile insects or capture of non-marked released flies can have deleterious effects and trigger costly control measures. These perceived risks encourage program managers to apply high radiation doses and high doses of marking dye. In addition, mass rearing factories are strategically located away from release areas to prevent escape of fertile individuals within eradicated areas, raising the need for lengthy transport. Such is the case for Anastrepha obliqua Macquart (Diptera: Tephritidae) released in mango producing areas of Mexico under an SIT-based eradication campaign. Here, we examined several standard quality-control parameters for mass-reared A. obliqua subjected to various time periods under hypoxia during transport, marked with different doses of fluorescent dye, and subjected to different radiation doses. Such factors were evaluated in isolation and in conjunction. Overall, long periods of hypoxia, high marking doses, and high radiation doses reduced the number of flying adults and increased the number of non-emerged pupae. Some quality-control parameters such as number of deformed adults, part-emerged pupae, and non-flying adults provided less informative guidance or redundant information of fly performance. Some tests such as mortality under stress and mating propensity in small cages were useless in detecting differences in quality among treatments for parameters evaluated during experiments. We discuss the quantity/safety-quality/performance conflict during eradication using SIT, propose different strategies according to different stages during eradication (management, suppression, eradication, outbreaks in free areas), where males irradiated at low doses and marked with low doses of dye can be released during early suppression, and examine the pertinence of carrying out different quality-control tests. © 2011 The Authors. Entomologia Experimentalis et Applicata © 2011 The Netherlands Entomological Society.
Liedo P.,Colegio de Mexico |
Orozco D.,Programa Moscamed Moscafrut |
Cruz-Lopez L.,Colegio de Mexico |
Quintero J.L.,Programa Moscamed Moscafrut |
And 4 more authors.
Journal of Applied Entomology | Year: 2013
The effect of post-teneral diets on the mating performance, pheromone production and longevity of sterile Anastrepha ludens and Anastrepha obliqua fruit flies (Diptera: Tephritidae) was investigated. Enriched pre-release diets improved male mating performance. Wild and sterile males fed on mango and orange fruits achieved the greatest copulatory success, followed by those fed on a 3:1 sugar:yeast (SY) diet. Males fed only on a sugar diet achieved the lowest number of matings. The mean numbers of copulas achieved by wild males were significantly higher than those by sterile males fed on a sugar-only diet, while there were no significant differences between wild males and sterile males fed on yeast diets. There was a trend of reduced mean number of copulas as the proportion of yeast was reduced in the diet, but differences were not significant. Pre-release diets had a significant effect on pheromone production. Males fed on a 3:1 SY diet produced the greatest amount of the three main pheromone components in A. ludens males and two major components in A. obliqua males, followed by males fed on fruits or pasteurized fruit juice. Males fed on a sugar only diet produced the lowest amounts. The longevity response to post-teneral diets was complex. The most yeast-rich diet and the poorest diet (sugar only) resulted in the lowest life expectancies. Flies fed on 24:1 SY diets showed the highest life expectancies for both males and females of the two species. Considering the tradeoffs between mating performance and longevity, the 24:1 SY diet would be recommended for programmes integrating the sterile insect technique, but the effects of these diets on field survival and dispersal still need to be investigated. © 2010 Blackwell Verlag, GmbH.