Quintero-Fong L.,Colegio de Mexico |
Toledo J.,Colegio de Mexico |
Ruiz L.,Colegio de Mexico |
Rendon P.,International Atomic Energy Agency |
And 3 more authors.
Bulletin of Entomological Research | Year: 2016
The sexual performance of Anastrepha ludens males of the Tapachula-7 genetic sexing strain, produced via selection based on mating success, was compared with that of males produced without selection in competition with wild males. Mating competition, development time, survival, mass-rearing quality parameters and pheromone production were compared. The results showed that selection based on mating competitiveness significantly improved the sexual performance of offspring. Development time, survival of larvae, pupae and adults, and weights of larvae and pupae increased with each selection cycle. Differences in the relative quantity of the pheromone compounds (Z)-3-nonenol and anastrephin were observed when comparing the parental males with the F4 and wild males. The implications of this colony management method on the sterile insect technique are discussed. Copyright © Cambridge University Press 2016
Murillo F.D.,Colegio de Mexico |
Murillo F.D.,Ursulo Galvan Institute of Technology |
Liedo P.,Colegio de Mexico |
Nieto-Lopez M.G.,Colegio de Mexico |
And 3 more authors.
Arthropod Structure and Development | Year: 2016
The morphology of the first instars of the Opiinae braconids Diachasmimorpha longicaudata, Doryctobracon areolatus, Doryctobracon crawfordi, Utetes anastrephae and Opius hirtus (the first is exotic, and the others are natives to Mexico), parasitoids of Anastrepha fruit flies, are described and compared. The possible implications on interspecific competition among these species are discussed. The most significant adaptations found were: (1) the mouth apparatus, where the large mandibles and fang-shaped maxillary lobes present in D. longicaudata and U. anastrephae larvae were absent in O. hirtus, D. areolatus and D. crawfordi larvae, and (2) the degree of mobility for exploration and escape, such as the lateral and caudal appendages that were only present in D. longicaudata (ventrolateral appendages in the base of the head capsule), U. anastrephae (caudal lobe with two appendages) and D. areolatus (caudal lobe with a round apex with a globular shape). The first instar larvae of the species D. longicaudata show morphological adaptations that apparently confer competitive advantages against the larvae of D. areolatus, D. crawfordi and O. hirtus. However, the first instar larvae of U. anastrephae show larger mandibles, an adaptation that could enable this species to resist competition from D. longicaudata. © 2016 Elsevier Ltd.
Diaz-Fleischer F.,University of Veracruz |
Galvez C.,Programa Moscafrut |
Montoya P.,Programa Moscafrut
Annals of the Entomological Society of America | Year: 2015
Parasitoid reproduction is often limited by disposable time for accessing hosts or egg availability. In the case of synovigenic parasitoids (which mature eggs during their lifetime), theory predicts that egg limitation is infrequent because they are able to replenish their egg supply throughout life. However, this theory does not contemplate the effect of superparasitism on egg load. If superparasitism is applied as a strategy for modulating offspring sex ratio, as extra eggs are normally deposited in each host, it is to be expected that females experience egg limitation, especially when host density is high. In this article, the effect of host density on egg load, oviposition, and sex ratio in the tephritid parasitoid Diachasmimorpha longicaudata (Ashmead) was investigated. Specifically we analyzed the rate of oviposition per host and adjustments in offspring sex ratio in response to changes in host density and how these affect egg load. Parasitoids maintained a similar rate of oviposition, independent of host densities, except when host density fluctuated between 25 and 5 hosts per day. Females increased their oviposition rate and parasitism when confronted with 5 hosts, after having experienced a density of 25 hosts. Offspring sex ratio was biased in favor of females at high host density and with increasing prevalence of superparasitism. Results suggest that parasitoids have a strategy for maintaining high egg load in order to mitigate egg limitation, and for adjusting oviposition rate and offspring sex ratio with reference to previous oviposition experience. © The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America.
Orozco-Davila D.,Programa Moscafrut |
Quintero-Fong L.,Programa Moscafrut
Journal of Economic Entomology | Year: 2015
A new adult diet formulation was evaluated for sterile Anastrepha ludens (Loew) and Anastrepha obliqua (Macquart) males at the emergence and release facility of fruit flies in Mexico. The formulation consists of hydrolyzed protein, sugar, juvenile hormone analogue methoprene, and water. The proportion of the ingredients between the solute (4% hydrolyzed protein and 96% sugar) and solvent (10% methoprene and 90% water) was 5:1. This new formulation was called the 1:24 formulation. The main objectives of this study were to develop a simple way to supply the 1:24 formulation to adults and to compare the sexual performance of these flies with the performance of flies fed a standard diet (called the Mubarqui formulation) used at the emergence and release facility of fruit flies in Mexico. The preparation, time, and cost also were evaluated. The results showed no significant differences in the sexual behaviors of the males (number of males mating, number of males calling, mating latency, and mating duration) between the 1:24 formulation and the Mubarqui formulation. However, the cost and the required preparation time are much lower for the 1:24 formulation process than for the Mubarqui formulation process. Based on these results, we recommend the 1:24 formulation as an additional adult diet option in the handling of sterile flies. Its application is practical and does not require changes in packaging systems. The contribution of our findings and their potential application to the improvement of the sterile insect technique are discussed. © The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved.
Meza J.S.,Programa Moscafrut |
Meza J.S.,University of Veracruz |
Diaz-Fleischer F.,University of Veracruz |
Sanchez-Velasquez L.R.,University of Veracruz |
And 4 more authors.
PLoS ONE | Year: 2014
Site-specific recombination technologies are powerful new tools for the manipulation of genomic DNA in insects that can improve transgenesis strategies such as targeting transgene insertions, allowing transgene cassette exchange and DNA mobilization for transgene stabilization. However, understanding the fitness cost implications of these manipulations for transgenic strain applications is critical. In this study independent piggyBac -mediated attP target-sites marked with DsRed were created in several genomic positions in the Mexican fruit fly, Anastrepha ludens. Two of these strains, one having an autosomal (attP-F7) and the other a Y-linked (attP-2-M6y) integration, exhibited fitness parameters (dynamic demography and sexual competitiveness) similar to wild type flies. These strains were thus selected for targeted insertion using, for the first time in mexfly, the phiC31-integrase recombination system to insert an additional EGFP-marked transgene to determine its effect on host strain fitness. Fitness tests showed that the integration event in the int-2-M6y recombinant strain had no significant effect, while the int-F7 recombinant strain exhibited significantly lower fitness relative to the original attP-F7 target-site host strain. These results indicate that while targeted transgene integrations can be achieved without an additional fitness cost, at some genomic positions insertion of additional DNA into a previously integrated transgene can have a significant negative effect. Thus, for targeted transgene insertions fitness costs must be evaluated both previous to and subsequent to new site-specific insertions in the target-site strain.