Programa de Pos graduacao em Ciencias e Biotecnologia

Niterói, Brazil

Programa de Pos graduacao em Ciencias e Biotecnologia

Niterói, Brazil
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Lyrio E.C.D.,Federal University of Fluminense | Campos-Souza I.C.,Federal University of Fluminense | Correa L.C.D.,Federal University of Fluminense | Lechuga G.C.,Programa de Pos graduacao em Ciencias e Biotecnologia | And 9 more authors.
Experimental Dermatology | Year: 2015

Leprosy is a chronic granulomatous disease caused by Mycobacterium leprae affecting the skin and peripheral nerves. Despite M. leprae invasion of the skin and keratinocytes importance in innate immunity, the interaction of these cells in vitro during M. leprae infection is poorly understood. Conventional and fluorescence optical microscopy, transmission electronic microscopy, flow cytometry and ELISA were used to study the in vitro interaction of M. leprae with the HaCaT human keratinocyte cell line. Keratinocytes uptake of M. leprae is described, and modulation of the surface expression of CD80 and CD209, cathelicidin expression and TNF-α and IL-1β production of human keratinocytes are compared with dendritic cells and macrophages during M. leprae interaction. This study demonstrated that M. leprae interaction with human keratinocytes enhanced expression of cathelicidin and greatly increased TNF-α production. The highest spontaneous expression of cathelicidin was by dendritic cells which are less susceptible to M. leprae infection. In contrast, keratinocytes displayed low spontaneous cathelicidin expression and were more susceptible to M. leprae infection than dendritic cells. The results show, for the first time, an active role for keratinocytes during infection by irradiated whole cells of M. leprae and the effect of vitamin D on this process. They also suggest that therapies which target cathelicidin modulation may provide novel approaches for treatment of leprosy. © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.


PubMed | Federal University of Fluminense, Hospital Universitario, Ghent University, Laboratorio Of Biologia Estrutural and Programa de Pos graduacao em Ciencias e Biotecnologia
Type: Journal Article | Journal: Experimental dermatology | Year: 2015

Leprosy is a chronic granulomatous disease caused by Mycobacterium leprae affecting the skin and peripheral nerves. Despite M.leprae invasion of the skin and keratinocytes importance in innate immunity, the interaction of these cells invitro during M.leprae infection is poorly understood. Conventional and fluorescence optical microscopy, transmission electronic microscopy, flow cytometry and ELISA were used to study the invitro interaction of M.leprae with the HaCaT human keratinocyte cell line. Keratinocytes uptake of M.leprae is described, and modulation of the surface expression of CD80 and CD209, cathelicidin expression and TNF- and IL-1 production of human keratinocytes are compared with dendritic cells and macrophages during M.leprae interaction. This study demonstrated that M.leprae interaction with human keratinocytes enhanced expression of cathelicidin and greatly increased TNF- production. The highest spontaneous expression of cathelicidin was by dendritic cells which are less susceptible to M.leprae infection. In contrast, keratinocytes displayed low spontaneous cathelicidin expression and were more susceptible to M.leprae infection than dendritic cells. The results show, for the first time, an active role for keratinocytes during infection by irradiated whole cells of M.leprae and the effect of vitamin D on this process. They also suggest that therapies which target cathelicidin modulation may provide novel approaches for treatment of leprosy.

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