Time filter

Source Type

Fronie A.,University of Medicine and Pharmacy of Craiova | Bunget A.,University of Medicine and Pharmacy of Craiova | Afrem E.,University of Medicine and Pharmacy of Craiova | Preotescu L.L.,Prof Dr Matei Bals National Institute For Infectious Diseases | And 4 more authors.
Romanian Journal of Morphology and Embryology | Year: 2013

Oral cavity cancer is a public health problem as the sixth leading cause of cancer worldwide. Most tumor lesions are detected in stage III and IV, leading to a poor prognosis, five-year survival rate ranging between 10% and 40%. Oral cancer etiology is multifactorial, known still incomplete. The main etiopathogenic factors are exposure to cigarette smoke and alcohol consumption. We conducted a retrospective study of oral cavity tumors hospitalized in 2008-2012 in Oral and Maxillofacial Surgery Clinic of the Emergency County Hospital of Craiova, Romania. Of 143 tumors of the oral cavity, 125 were malignant, and of these, 115 (92%) were represented by squamous cell carcinoma. Tumor lesions were more common in males (69%), patients from rural areas (64%) and those over 50-year-old (87.71%).


Baicus C.,University of Bucharest | Baicus C.,Clinical Research Unit RECIF Reseau dEpidemiologie Clinique International Francophone | Hristea A.,Clinical Research Unit RECIF Reseau dEpidemiologie Clinique International Francophone | Hristea A.,Prof Dr Matei Bals National Institute For Infectious Diseases | And 2 more authors.
Revista Romana de Medicina de Laborator | Year: 2011

Receiver operator characteristic (ROC) curves are used in order to assess the accuracy of diagnostic tests whose results are continuous numeric variables. This curve is a graph of the sensitivity (or true positive rate) on the Y-axis as a function of 1-specificity (the false positive rate) on the Y-axis. ROC curves have multiple utilizations: 1. The comparison of more tests for the same disease (bigger the area under the ROC curve (AU - ROC), better the test; an AUROC of 1 means a perfect test, while an AUROC of 0.5 means a useless test; 2. The choice of a cut-offpoint (in case of a test with a big AUROC, one can choose the closest point to the upper left corner of the graph, in order to have both a good sensitivity and a good specificity); 3. It shows how, for the same diagnostic test, there is a negotiation between sensitivity and specificity so that, for a cut-off with a very good sensitivity it will be a weak specificity and the reverse.


Popescu G.A.,Prof Dr Matei Bals National Institute For Infectious Diseases | Otelea D.,National Diagnostics | Gavriliu L.C.,Prof Dr Matei Bals National Institute For Infectious Diseases | Neaga E.,National Diagnostics | And 3 more authors.
Journal of Medical Virology | Year: 2013

Epidemiological analyses indicate a decreasing level of hepatitis D (HDV) infections in most developed countries during the last 15 years. Romania, however, is one of the European countries that still has high morbidity from HDV; this study was performed in order to estimate the HDV prevalence in the Bucharest area. Three thousand four hundred sixty-one hepatitis B (HBV) infected patients were invited to participate and 1,094 were recruited. Serum anti-HDV IgG was detected in 223 patients indicating a hepatitis D seroprevalence of 20.4% (95% CI=18.1-22.9) in patients chronically infected with HBV, less than that seen in previous studies. Seroprevalence was not gender related, but patients over 40 years were more likely to have anti-HDV antibodies, RR=1.9 (1.2; 3.0). Detectable hepatitis D viraemia was found in 67.7% of the patients who were positive for anti-HDV. The HDV genotype was characterized for 40 isolates; all were very similar and belonged to genotype 1. Serum HBV-DNA was detectable less frequently in patients positive for anti-HDV than in patients infected with HBV alone: 68.5% versus 89.3%, OR 3.9 (1.7; 10.0), but the extent of this HDV replicative dominance varies with the sensitivity of the HBV-DNA detection. 19.3% of the subjects who tested positive for anti-HDV IgG had a HBV-DNA level higher than four logs. This high prevalence prompts the need for better HBV vaccination coverage and measures to prevent super infection with HDV in patients infected with HBV. J. Med. Virol. 85:769-774, 2013. © 2013 Wiley Periodicals, Inc.


Hristea A.,Prof Dr Matei Bals National Institute For Infectious Diseases | Otelea D.,Prof Dr Matei Bals National Institute For Infectious Diseases | Paraschiv S.,Prof Dr Matei Bals National Institute For Infectious Diseases | MacRi A.,Marius Nasta National Institute of Pneumology | And 5 more authors.
Indian Journal of Medical Microbiology | Year: 2010

Objective: The objective of our study was to evaluate the use of a real-time polymerase chain reaction (PCR)-based technique for the prediction of phenotypic resistance of Mycobacterium tuberculosis. Materials and Methods: We tested 67 M tuberculosis strains (26 drug resistant and 41 drug susceptible) using a method recommended for the LightCycler platform. The susceptibility testing was performed by the absolute concentration method. For rifampin resistance, two regions of the rpoB gene were targeted, while for identification of isoniazid resistance, we searched for mutations in katG and inhA genes. Results: The sensitivity and specificity of this method for rapid detection of mutations for isoniazid resistance were 96% (95% CI: 88% to 100%) and 95% (95% CI: 89% to 100%), respectively. For detection of rifampin resistance, the sensitivity and specificity were 92% (95% CI: 81% to 100%) and 74% (95% CI: 61% to 87%), respectively. The main isoniazid resistance mechanism identified in our isolates is related to changes in the katG gene that encodes catalase. We found that for rifampin resistance the concordance between the predicted and observed phenotype was less than satisfactory. Conclusions: Using this method, the best accuracy for genotyping compared with phenotypic resistance testing was obtained for detecting isoniazid resistance mutations. Although real-time PCR assay may be a valuable diagnostic tool, it is not yet completely satisfactory for detection of drug resistance mutations in M tuberculosis.


Hristea A.,Prof Dr Matei Bals National Institute For Infectious Diseases
Chirurgia (Bucharest, Romania : 1990) | Year: 2010

Primary tuberculous tenosynovitis is a rare manifestation of extraspinal musculoskeletal tuberculosis. The diagnosis may be easily delayed because of its nonspecific clinical signs. We report a case of culture-proven tuberculous tenosynovitis of the extensor carpi ulnaris tendon and common extensor tendon in a 68-year-old female without concomitant pulmonary tuberculosis, nor documented immunodeficiency. The diagnosis was initially overlooked due to the lack of appropriate histological and bacteriological analyses and the lesion recurred after surgery. MR imaging represents the most accurate method in making the diagnosis, but has no diagnostic specificity in regard to tuberculosis, therefore surgical biopsy is strongly recommended. The patient had a favorable clinical response after a combination of excision and appropriate antituberculous therapy for sensitive Mycobacterium tuberculosis. We emphasize the need for an increased awareness and high index of suspicion of tuberculosis in all cases of a chronic orrecurrent abscess in the extremities, not only in patients living in endemic areas but also in those who have emigrated from regions with a high prevalence of tuberculosis.


Jipa R.,Prof Dr Matei Bals National Institute For Infectious Diseases | Hristea A.,Prof Dr Matei Bals National Institute For Infectious Diseases | Hristea A.,Carol Davila University of Medicine and Pharmacy | Arama V.,Prof Dr Matei Bals National Institute For Infectious Diseases | Arama V.,Carol Davila University of Medicine and Pharmacy
Gineco.eu | Year: 2013

Group B streptococcus (GBS) is one of the main cause of neonatal infections, especially in preterm neonates. GBS presence, both in mothers and neonates, has a remarkable impact on morbidity and mortality. Screening for GBS infection in mothers during pregnancy in the third trimester, monthly urinalysis and promptly initiation of antibiotic therapy intrapartum will lead to a significant decrease of early onset GBS disease.


PubMed | Prof Dr Matei Bals National Institute for Infectious Diseases
Type: Comparative Study | Journal: Indian journal of medical microbiology | Year: 2010

The objective of our study was to evaluate the use of a real-time polymerase chain reaction (PCR)-based technique for the prediction of phenotypic resistance of Mycobacterium tuberculosis.We tested 67 M tuberculosis strains (26 drug resistant and 41 drug susceptible) using a method recommended for the LightCycler platform. The susceptibility testing was performed by the absolute concentration method. For rifampin resistance, two regions of the rpoB gene were targeted, while for identification of isoniazid resistance, we searched for mutations in katG and inhA genes.The sensitivity and specificity of this method for rapid detection of mutations for isoniazid resistance were 96% (95% CI: 88% to 100%) and 95% (95% CI: 89% to 100%), respectively. For detection of rifampin resistance, the sensitivity and specificity were 92% (95% CI: 81% to 100%) and 74% (95% CI: 61% to 87%), respectively. The main isoniazid resistance mechanism identified in our isolates is related to changes in the katG gene that encodes catalase. We found that for rifampin resistance the concordance between the predicted and observed phenotype was less than satisfactory.Using this method, the best accuracy for genotyping compared with phenotypic resistance testing was obtained for detecting isoniazid resistance mutations. Although real-time PCR assay may be a valuable diagnostic tool, it is not yet completely satisfactory for detection of drug resistance mutations in M tuberculosis.


PubMed | Prof Dr Matei Bals National Institute For Infectious Diseases
Type: Journal Article | Journal: Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie | Year: 2016

The study aims to compare two aspects concerning the diagnosis of acquired immune deficiency syndrome (AIDS)-associated central nervous system (CNS) pathology (neuroAIDS): clinical diagnoses issued ante mortem with pathology results issued post mortem. The group of 39 human immunodeficiency virus (HIV)-positive patients was created over 23 years and is limited by marked heterogeneity. The enrolled cases were treated at the Prof. Dr. Matei Bals National Institute for Infectious Diseases, Bucharest, Romania, deceased due to AIDS-related complications and underwent brain necropsies performed in the Pathology Laboratory at the Colentina Clinical Hospital, Bucharest. The level of superposition between clinical and the necroptic diagnoses of neurological AIDS-associated diseases was: 60% for progressive multifocal leukoencephalopathy (PML), 50% for cerebral cryptococcosis, 33% for cerebral toxoplasmosis, 20% for cerebral lymphoma, null for cerebral tuberculosis, HIV encephalopathy (HIVE), neurosyphilis and cytomegalovirus cerebral infection. Half of the cases without an AIDS-associated CNS lesion were previously clinically overdiagnosed. We observed that the rate of overdiagnosis concerning an AIDS-associated cerebral illness has risen from 36% in 1993 to 124% in 2015, an elevation with statistical relevance [p=0.037, confidence interval (CI) 95%]. The rate of underdiagnosis has slowly risen from 24% in 1993 to 40% in 2015, however, with no statistical relevance. The rate of clinical confirmation has been stagnant in linear regression from 1993 to 2015. The results of our study reveal a gap between ante mortem and post mortem diagnoses, with many instances of overdiagnosis and underdiagnosis of several major AIDS-associated CNS illnesses, highlighting the need for a more detailed, multidisciplinary approach of neuroAIDS.


PubMed | Prof Dr Matei Bals National Institute For Infectious Diseases and Prof Dr Marius Nasta Institute For Respiratory Medicine
Type: Journal Article | Journal: Journal of medicine and life | Year: 2014

Pneumonias are usually acute infectious processes of the pulmonary parenchyma, being the result of the overcome of the natural defence mechanisms of the human organism, which lead to the penetration, persistence and multiplication of a biologic agent (which has become a pathogen agent) within the lungs. This is a phenomena which generates local manifestations (inflammation) and systemic manifestations, their variable dimension (which is conditioned by the state of the host and/or the virulence of the pathogen agent) explaining the clinical, paraclinical and biological dimensions of the phenomena. The current material deals on one hand with the phenomena which takes part in the formation of the heterogeneous spectrum of the pneumonias and, on the other hand, we will demonstrate the manner in which the expansion and the severity of the infectious phenomena depend on the virulence of the etiologic agent, on the manner in which the defence mechanisms have been overcome, and also on the biological state of the invaded organism.


PubMed | Prof Dr Matei Bals National Institute For Infectious Diseases
Type: Case Reports | Journal: Chirurgia (Bucharest, Romania : 1990) | Year: 2010

Primary tuberculous tenosynovitis is a rare manifestation of extraspinal musculoskeletal tuberculosis. The diagnosis may be easily delayed because of its nonspecific clinical signs. We report a case of culture-proven tuberculous tenosynovitis of the extensor carpi ulnaris tendon and common extensor tendon in a 68-year-old female without concomitant pulmonary tuberculosis, nor documented immunodeficiency. The diagnosis was initially overlooked due to the lack of appropriate histological and bacteriological analyses and the lesion recurred after surgery. MR imaging represents the most accurate method in making the diagnosis, but has no diagnostic specificity in regard to tuberculosis, therefore surgical biopsy is strongly recommended. The patient had a favorable clinical response after a combination of excision and appropriate antituberculous therapy for sensitive Mycobacterium tuberculosis. We emphasize the need for an increased awareness and high index of suspicion of tuberculosis in all cases of a chronic orrecurrent abscess in the extremities, not only in patients living in endemic areas but also in those who have emigrated from regions with a high prevalence of tuberculosis.

Loading Prof Dr Matei Bals National Institute For Infectious Diseases collaborators
Loading Prof Dr Matei Bals National Institute For Infectious Diseases collaborators