Mihalcea C.E.,Prof Dr Alexandru Trestioreanu Oncological Institute |
Morosanu A.-M.,Institute of Biology Bucharest of Romanian Academy |
Murarasu D.,Prof Dr Alexandru Trestioreanu Oncological Institute |
Puiu L.,Prof Dr Alexandru Trestioreanu Oncological Institute |
And 3 more authors.
Romanian Journal of Morphology and Embryology | Year: 2015
Electron microscopic investigations of invasive mammary carcinoma tumors revealed that intercellular junctions, namely desmosomes are severely altered; some desmosomes became internalized. Tumor cells, especially by their invadopodia, generate and disseminate membrane vesicles, including exosomes, inside of peritumoral stroma. Telocytes, a new described interstitial/stromal cell phenotype, considered to play important roles in cell signaling, exhibited a reduced number of hetero-cellular contacts, which suggests a possible perturbation of tissular homeostasis modulation. Signaling PIK3/Akt pathway plays an important role both in carcinogenesis and in proliferation, differentiation, and cell survival. Alteration of this pathway has been observed in many human cancers, often involving an increase in the activity of PIK3CA, p110α catalytic subunit of PI3K. Our study confirms the high prevalence of PIK3CA mutations in breast cancer. In accordance with the results of the largest previous studies, 87.5% of mutations detected by DNA direct sequencing were hot spot mutations, most of them located in the kinase domain. High percentage of mutations detected by high-resolution melting makes the assay an attractive choice for mutation scanning, especially, in samples with low percentage of tumor cell. © 2015 ROMANIAN ACADEMY PUBLISHING HOUSE. All rights reserved.
Iorga C.,Carol Davila University of Medicine and Pharmacy |
Cirimbei S.,Prof Dr Alexandru Trestioreanu Oncological Institute |
Strambu V.,Carol Davila University of Medicine and Pharmacy |
Popa F.,Carol Davila University of Medicine and Pharmacy
Journal of medicine and life | Year: 2013
Laparoscopic cholecystectomy is the standard treatment for patients requiring gallbladder removal. Although the advantages of the laparoscopic technique are widely accepted, the introduction of this technique has doubled the rate of iatrogenic lesions of extrahepatic bile ducts. Research methods for biliary tree also evolved, but intraoperative cholangiography, the traditional exploring method used for the biliary tree in classic cholecystectomy remains a valuable investigation in the laparoscopic technique. We performed a retrospective study on a group of patients who underwent cholecystectomy (laparoscopic or classic). Of these, intraoperative cholangiography was performed on a total of 108 patients. Patients who underwent cholangiography motivated by preoperative investigations were excluded from the group and the study operates on patients in whom the decision to perform cholangiography was taken during surgery (45 cases). We have analyzed the criteria that led to the motivation investigation (dilated cystic duct, suspected biliary duct stones, suspicion of iatrogenic biliary injury) results and subsequent therapeutic conduct. The results showed that in 90% of the patients, the suspected diagnosis was confirmed by cholangiography (10 cases with normal cholangiography aspect, oddita 9 cases, 11 cases with bile duct stones, 2 cases with biliary tumor and 13 cases of iatrogenic biliary injury). In conclusion, the decision to perform intraoperative cholangiography proved salutary, the suspected diagnosis was confirmed and the course of treatment was adjusted accordingly.