Piotrowska-Tomala K.K.,Polish Academy of Sciences |
Bah M.M.,Polish Academy of Sciences |
Jankowska K.,Polish Academy of Sciences |
Lukasik K.,Polish Academy of Sciences |
And 3 more authors.
Domestic Animal Endocrinology | Year: 2015
The aim of the study was to determine the effects of lipopolysaccharide (LPS), tumor necrosis factor alpha (TNF), interleukin-1-alpha (IL-1α), and nitric oxide donor (NONOate) on both invivo and invitro secretion of prostaglandin (PG)E2, PGF2α, leukotriene (LT)B4, and LTC4 by the bovine mammary gland. In the first experiment, tissues isolated from the teat cavity and lactiferous sinus were treated invitro with LPS (10ng/mL), TNF (10ng/mL), IL-1α (10ng/mL), NONOate (10-4M), and the combination of TNF + IL-1α + NONOate for 4 or 8h. PGE2 or PGF2α secretion was stimulated by all treatments (P < 0.05) excepting NONOate alone, which did not stimulate PGF2α secretion. Moreover, all factors increased LTB4 and LTC4 secretion (P < 0.05). In the second experiment, mastitis was experimentally mimicked invivo by repeated (12h apart) intramammary infusions (5mL) of (1) sterile saline; (2) 250-μg LPS; (3) 1-μg/mL TNF; (4) 1-μg/mL IL-1α (5) 12.8-μg/mL NONOate; and (6) TNF + IL-1α + NONOate into 2 udder quarters. All infused factors changed PGE2, 13,14-dihydro,15-keto-PGF2α, and LT concentrations in blood plasma collected from the caudal vena cava, the caudal superficial epigastric (milk) vein, the jugular vein, and the abdominal aorta (P < 0.05). In summary, LPS and other inflammatory mastitis mediators modulate PG and LT secretion by bovine mammary gland in both invivo and invitro studies. © 2015 Elsevier Inc.