Raoofi A.,University of Tehran |
Hemmatzadeh F.,University of Adelaide |
Ghanaei A.M.,Private Veterinary Clinic
Journal of Camel Practice and Research | Year: 2012
Serological survey was carried out to detect antibodies in dromedary camels against bovine herpesvirus type-1 (BHV-1) and Mycobacterium avium paratuberculosis (Map) in Iran. Serum samples were collected from camels at Khorein abattoir in suburbs of Tehran. Using the serum neutralisation test (SNT), 137 samples were examined and no antibodies to BHV-1 were detected. A modified camelid-specific serum antibody assay was used and 2 of 90 camels (2.2%) were found positive for Map antibodies. It is concluded that camels probably may play a role in the persistence and transmission of Map infection among ruminants in Iran. This is the first report for the detection of antibodies in dromedary camels against BHV-1 and Map in Iran.
Ciocca L.,University of Bologna |
Donati D.,Rizzoli Orthopaedic Institute |
Ragazzini S.,University of Bologna |
Dozza B.,Rizzoli Orthopaedic Institute |
And 9 more authors.
BioMed Research International | Year: 2013
Purpose. This study evaluated the efficacy of a regenerative approach using mesenchymal stem cells (MSCs) and CAD-CAM customized pure and porous hydroxyapatite (HA) scaffolds to replace the temporomandibular joint (TMJ) condyle. Methods. Pure HA scaffolds with a 70% total porosity volume were prototyped using CAD-CAM technology to replace the two temporomandibular condyles (left and right) of the same animal. MSCs were derived from the aspirated iliac crest bone marrow, and platelets were obtained from the venous blood of the sheep. Custom-made surgical guides were created by direct metal laser sintering and were used to export the virtual planning of the bone cut lines into the surgical environment. Sheep were sacrificed 4 months postoperatively. The HA scaffolds were explanted, histological specimens were prepared, and histomorphometric analysis was performed. Results. Analysis of the porosity reduction for apposition of newly formed bone showed a statistically significant difference in bone formation between condyles loaded with MSC and condyles without (P<0.05). The bone ingrowth (BI) relative values of split-mouth comparison (right versus left side) showed a significant difference between condyles with and without MSCs (P<0.05). Analysis of the test and control sides in the same animal using a split-mouth study design was performed; the condyle with MSCs showed greater bone formation. Conclusion. The split-mouth design confirmed an increment of bone regeneration into the HA scaffold of up to 797% upon application of MSCs. © 2013 L. Ciocca et al.
Forro A.,University of Veterinary Medicine Hannover |
Tsousis G.,Aristotle University of Thessaloniki |
Beindorff N.,Charité - Medical University of Berlin |
Sharifi R.,University of Gottingen |
And 2 more authors.
Journal of Dairy Science | Year: 2012
The objective of this study was to evaluate the effects of different Ovsynch protocols combined with progesterone (P4) supplementation after artificial insemination (AI) of Holstein-Friesian cows. Cows were randomly synchronized at 52 to 63 d after parturition with either the classical Ovsynch protocol (GnRH on d 0, PGF2α on d 7, GnRH 48h after PGF2α) or with a modified Ovsynch protocol (second GnRH 60h after PGF2α). On d 4 after timed AI (TAI), the cows were blocked by parity and randomly divided into 2 groups. Half of the cows were supplemented with P4 (P4+) by applying a P4-releasing intravaginal device intravaginally for 14 d, whereas the other half remained untreated (P4-). In 50% of randomly chosen cows, plasma P4 was measured on d 4, 5, and 18 after TAI. Sonographic pregnancy diagnosis was performed on d 33 after TAI in a total of 398 cows. Health status and body condition score (BCS) of all cows were examined at several stages of the study. Cows in the modified Ovsynch protocol tended to have higher P4 values on d 4 after TAI than cows in the classical Ovsynch protocol (2.1±0.2 vs. 1.6±0.2ng/mL), but no difference in pregnancy per AI (P/AI) was observed between the 2 Ovsynch protocols (38.4% vs. 44.1%). Independent of the Ovsynch protocols, P4+ cows tended to have higher P/AI compared with P4- cows (44.4% vs. 38.1%). The retention of fetal membranes and BCS at the time of insemination affected P/AI. Moreover, an interaction between BCS at the time of insemination and P4 supplementation was apparent; that is, the difference in P/AI between P4+ and P4- cows was significant in cows with BCS ≥3.25. Progesterone-supplemented cows showed higher P4 values on d 5 (4.9±0.2 vs. 2.6±0.2) and d 18 (7.8±0.2 vs. 6.3±0.2) after TAI, respectively. In conclusion, the elongation of the time interval between the injections of PGF2α and the second GnRH from 48 to 60h had no effect on P/AI. Progesterone supplementation after insemination improved the P/AI of the Ovsynch protocols, but this effect was more apparent in cows with BCS ≥3.25. © 2012 American Dairy Science Association.
Raoofi A.,University of Tehran |
Namjoo A.,Islamic Azad University |
Karimi A.H.,Private Veterinary Clinic |
Alizadeh Esfahani M.,Blood Transfusion Laboratory of Esfahan
Small Ruminant Research | Year: 2012
The aim of this study was to investigate the clinical signs, hematological changes and pathological findings caused by ingestion of plastic ropes in goats. Ten female goats under one year of age were used in this study. They were equally divided into two groups: test (n= 5) and control (n= 5). In the test group, each goat was fed with an 80. cm long plastic rope marked with radiographic markers. All animals from both groups were examined on alternate days during the 50 days of experiment. Blood samples were collected from both groups on days 1 and 50.During the experiment no clinical signs were observed. The rumens in the test group were radiographed to evaluate the location of the rope. All animals were slaughtered on the day 50. Ingested plastic rope did not produce any significant changes in mean values of packed cell volume, white blood cells, differential leukocyte count, and serum calcium, phosphorus, magnesium, sodium, potassium, chloride, glucose, albumin, fibrinogen and total protein concentrations.At necropsy examination most lesions were found in ventral sac of rumen. Microscopic examination revealed a significant increase (p< 0.05) in the thickness of corneous layer of ventral sac, and muscular layer of ventral and caudoventral sacs. A significant reduction (p< 0.05) in the thickness of mucosal and submucosal layers of the ventral and cranial sacs was seen. Hyperkeratosis, parakeratosis and severe hydropic degeneration on mucosal layer and increase in the number and dilation of lymph vessels in the serous layer were observed in the test group. © 2011 Elsevier B.V.
Szostek A.Z.,Institute of Animal Reproduction and Food Research |
Lukasik K.,Institute of Animal Reproduction and Food Research |
Majewska M.,Institute of Animal Reproduction and Food Research |
Bah M.M.,Institute of Animal Reproduction and Food Research |
And 3 more authors.
Domestic Animal Endocrinology | Year: 2011
Tumor necrosis factor-α (TNF-α) is involved in the tissue remodeling that occurs in the corpus luteum (CL) during its development and regression. This cytokine is also implicated in the regulation of reproduction by its actions on ovarian steroidogenic cells. The aim of this study was to examine the influence of TNF-α on (1) progesterone (P4) output by the bovine CL and on (2) the responsiveness of the CL to LH or prostaglandin E2 (PGE2) in vitro. In experiment 1, CL (days 8 to 10 of the estrous cycle) were perfused by using an in vitro microdialysis system with TNF-α (0.1, 0.5, or 1 μg/mL) alone or with TNF-α (1 μg/mL) followed by LH (1000 ng/mL) or PGE2 (2 × 10-5 M). Basal P4 release (P < 0.05) was increased by TNF-α (0.5 or 1 μg/mL). Moreover, TNF-α (1 μg/mL) inhibited the stimulatory effect of LH or PGE2 on P4 output (P < 0.05). In experiment 2, 4 h after intrauterine infusion of TNF-α (0.01 μg/mL or 1 μg/mL), CL (days 8 to 10 of the estrous cycle) were collected by colpotomy, cultured, and stimulated with LH (10 ng/mL) or PGE2 (10-6 M). Intrauterine infusion of TNF-α at a concentration of 1 μg/mL increased basal P4 output by CL (P < 0.05). Moreover, the intrauterine infusion of TNF-α at a concentration of 0.01 μg/mL inhibited the stimulatory effect of LH or PGE2 on P4 output (P < 0.05). These results indicate that TNF-α (1) does not have an effect on the autonomous, pulsatile release of P4; (2) increases P4 secretion by bovine CL with increasing doses, and (3) reduces in a dose-dependent manner the responsiveness of CL to luteotropic factors both directly (after infusion to CL) and indirectly (after intrauterine infusion). © 2011 Elsevier Inc.
Szczubial M.,Lublin University of Life Sciences |
Dabrowski R.,Lublin University of Life Sciences |
Bochniarz M.,Lublin University of Life Sciences |
Komar M.,Private Veterinary Clinic
Theriogenology | Year: 2013
The aim of the study was to determine the influence of the duration of the expulsive stage of parturition on the occurrence of postpartum oxidative stress in sows with uncomplicated, spontaneous farrowings. Twenty-five pregnant gilts were divided into three groups on the basis of duration of the expulsive stage of farrowing: (I) duration of the expulsive stage was below 3 hours; (II) duration of the expulsive stage ranged from 3 to 6 hours; (III) duration of the expulsive stage was longer than 6 hours. Blood samples were collected at 24 to 48 hours before and 24 hours after parturition. As indicators of alterations in the redox state, we quantified the enzymatic activity of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT), as well as the blood levels of glutathione (GSH), thiobarbituric acid reactive substances (TBARS), and sulfhydryl groups (SH groups). In group III, it was found that erythrocyte activity of CAT (63.89 ± 6.70 vs. 53.18 ± 2.32 U/g Hb), as well as plasma GSH concentration (0.088 ± 0.020 vs. 0.045 ± 0.024 mmol/g protein) and SH groups content (5.045 ± 1.256 vs. 3.383 ± 0.430 μmol/g protein) decreased significantly (P < 0.05) at 24 hours after parturition, compared with their values during the last 48 hours before parturition. The concentration of TBARS increased not significantly, although markedly at 24 hours postpartum in group III (0.124 ± 0.014 vs. 0.153 ± 0.031 μmol/g protein). The results indicate that uncomplicated, spontaneous parturition can lead to the occurrence of oxidative stress during the early postparturient period in sows, the intensity of which is related to the duration of the expulsive stage. © 2013 Elsevier Inc.
Bahrami S.,Shahid Chamran University |
Ghadrdan A.R.,Shahid Chamran University |
Mirabdollahi S.M.,Shahid Chamran University |
Fayed M.R.,Private Veterinary Clinic
Tropical Biomedicine | Year: 2014
A total of 105 blood samples from healthy horses from different stables in Yazd province, center of Iran, were examined for the presence of Theileria equi infection using parasitological and molecular methods. Out of the 105 samples, the parasitological method detected T. equi infection in 5 (4.76%) cases while the PCR method gave 24 (22.86%) positive results. Age, gender and breed were not determined as risk factors for T. equi infection in this study. Since blood samples were taken from healthy animals, this implies that 22.86% of horses had subclinical theileriosis in the current study. In conclusion, this study demonstrated that T. equi is present in horses in the center of Iran. Despite the healthy appearance of horses, these carrier animals can transmit the parasites to ticks and are a potential continuous source for maintaining and disseminating the organisms to the horse population. We concluded that it is important to make further studies on definitive host and vectors in the respective areas.
Raoofi A.,University of Tehran |
Asadi F.,University of Tehran |
Mardjanmehr S.H.,University of Tehran |
Kazempoor R.,Private Veterinary Clinic
Food and Chemical Toxicology | Year: 2010
Ten ewe lambs (median age 11 months and average weight 29.2 ± 2.5 kg) were used in the present study. They were divided into two groups: test (n = 5) and control (n = 5). Housing and all diets were identical. In the test group vitamin A was injected into the thigh muscle at a daily dose of 5000 IU/kg body weight for 16 days. The average final body weight of sheep in the test group was significantly (P < 0.05) less than the control group. All animals were slaughtered at day 17. The lambs' feet were X-rayed to evaluate any difference for radiographic signs between test and control groups. No significant differences were seen for PCV, WBC, differential leukocyte count, and total serum protein between groups. There were no significant differences for serum AST, ALT, and ALP activities and serum calcium, inorganic phosphate, and magnesium concentrations between groups. Histological examination revealed an increased number of lipid droplets in the cytoplasm of the stellate cells of the liver in the test group. The results showed that daily administrations of vitamin A approximately 150 times greater than the daily requirement were well tolerated by sheep. © 2009 Elsevier Ltd. All rights reserved.