Private Laboratory

Brunow, Germany

Private Laboratory

Brunow, Germany
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Kreutz M.,Private Laboratory | Stoeck T.,University of Kaiserslautern | Foissner W.,University of Salzburg
Journal of Eukaryotic Microbiology | Year: 2012

We redescribe Paramecium chlorelligerum, a forgotten species, which Kahl (Tierwelt Dtl., 1935, 30:651) briefly but precisely described in the addendum to his ciliate monographs as a Paramecium with symbiotic green algae. The redescription is based on classical morphological methods and the analysis of the small subunit (SSU) rDNA. Morphologically, P. chlorelligerum differs from P. (C.) bursaria, the second green species in the genus, by having a special swimming shape, the length of the caudal cilia, the size of the micronucleus, the size of the symbiotic algae, the contractile vacuoles (with collecting vesicles vs. collecting canals), and the number of excretory pores/contractile vacuole (1 vs. 2-3). The molecular investigations show that P. chlorelligerum forms a distinct branch distant from the P. (Chloroparamecium) bursaria clade. Thus, we classify P. chlorelligerum in a new subgenus: Paramecium (Viridoparamecium) chlorelligerum. The symbiotic alga belongs to the little-known genus Meyerella, as yet recorded only from the plankton of a North American lake. © 2012 The Author(s) Journal of Eukaryotic Microbiology © 2012 International Society of Protistologists.

Westermeier R.,Austral University of Chile | Patino D.J.,Austral University of Chile | Muller H.,Private Laboratory | Muller D.G.,University of Konstanz
Journal of Applied Phycology | Year: 2010

Macrocystis is an important marine resource in Chile, with severe problems of over-exploitation. Our study describes genetic materials and techniques for a further improvement of laboratory-based mariculture. For a systematic hybridization program we have selected one pair (cultivar) of gametophytes with favorable somatic and reproductive characteristics from each of seven localities in southern Chile. Sporophytes from all 49 crosses were grown for 10 weeks to seedling size. We report here that sporophytes from sympatric parents (intra-cultivar matings) grow to different length, depending on the locality and, importantly, that sporophytes from several inter-cultivar crossings show superior growth, suggesting heterosis with symmetric or asymmetric reciprocity. The genetic materials and techniques described here, together with our newly developed standardized seedling production protocols now available, constitute a significant step towards domestication of Macrocystis in analogy to terrestrial agriculture. © Springer Science+Business Media B.V. 2009.

De Freitas C.F.N.P.,Private clinic | Mulinari-Brenner F.,Federal University of Paraná | Fontana H.R.,Catholic University of Curitiba | Gentili A.C.,Private laboratory | Hammerschmidt M.,Private clinic
Anais Brasileiros de Dermatologia | Year: 2013

Ichthyoses are a common group of keratinization disorders. A non-inflammatory generalized persistent skin desquamation is observed. It is characterized by increased cell turnover, thickening of the stratum corneum and functional changes of sebaceous and sweat glands. All of these favor fungal proliferation. Dermatophytes may infect skin, hair and nails causing ringworm or tinea. They have the ability to obtain nutrients from keratinized material. One of its most prevalent genera is Trichophyton rubrum. Although tineas and ichthyoses are quite common, the association of the two entities is rarely reported in the literature. Three cases of ichthyosis associated with widespread infection by T. rubrum are presented. Resistance to several antifungal treatments was responsible for worsening of ichthyosis signs and symptoms. © 2013 by Anais Brasileiros de Dermatologia.

Cruz G.,Private Laboratory
Journal of Chemical Education | Year: 2013

The use of boric acid in the Kjeldahl determination of nitrogen is a variant of the original method widely applied in many laboratories all over the world. Its use is recommended by control organizations such as ISO, IDF, and EPA because it yields reliable and accurate results. However, the chemical principles the method is based on are not detailed in most analytical textbooks, perhaps because the nature of boric acid is not known in depth. To introduce the reader or student to the chemistry of boric acid, this article first describes the differences in acid-base behavior of concentrated and diluted boric acid by simple calculations and experimental pH measurements. Next, polyborate structures and their respective dissociation constants are shown to explain the differences found in previous pH measurements. In the second part of the article, the determination of ammonia with HCl is made in concentrated and diluted boric acid to reproduce the final Kjeldahl titration when two different volumes of ammonia distillate are collected. From the previous information, the apparently paradoxical results of the titration curves are explained in a qualitative manner. The third part consists of a study of systematic errors produced when colored indicators are used in the final Kjeldahl titration in concentrated and diluted boric, respectively. © 2013 The American Chemical Society and Division of Chemical Education, Inc.

Minasyan H.A.,Private Laboratory
International Reviews of Immunology | Year: 2014

Leukocytes can't perform phagocytosis in blood stream. Blood velocity prevents phagocytosis because there is no time for leukocyte to recognize and catch bacteria. Bloodstream clearance from pathogens is performed by erythrocytes. During motion in bloodstream erythrocytes become charged by triboelectric effect. This charge attracts bacteria and fixes them on the surface of erythrocyte, then bacteria are engulfed and killed by hemoglobin oxygen. In bloodstream, leukocyte thin-wrinkled elastic membrane can't be charged by triboelectric effect and so leukocyte can't catch bacteria by means of electrostatic attraction force. Leukocytes engulf and kill bacteria out of blood circulatory system: in tissues, lymph nodes, slow velocity lymph, etc. Erythrocyte and leukocyte are bactericidal partners: the first kills bacteria in bloodstream, the second kills them locally, out of blood circulation. © 2014 Informa Healthcare USA, Inc.

Villalon G.C.,Private Laboratory
Journal of Chemical Education | Year: 2013

The modification of a plastic bottle to be adapted to an electronic digital buret is described. A hole is made at the bottom of the bottle that allows a little container with the titrant to be placed inside the bottle. Determinations with relatively small volume of titrant can be made with this system. This design is especially useful for titrations with expensive or unstable reagents, thus, saving costs and space storage in the laboratory. © 2013 The American Chemical Society and Division of Chemical Education, Inc.

Ibba F.,University of Milan | Ibba F.,Private Laboratory | Lepri E.,University of Perugia | Veronesi F.,University of Perugia | And 2 more authors.
Journal of Feline Medicine and Surgery | Year: 2014

A gastric nodule was found in a cat examined following a car accident. Cytological examination showed a mixed, mainly eosinophilic, inflammation with reactive fibroblasts and ovoidal elements resembling nematode eggs. The cat was euthanased because of rapid worsening of clinical signs due to secondary injuries, and the gastric nodule was excised for examination. The intramural mass was smooth and spherical, with an intact mucosal surface and a little opening from which several reddish worms were extruding. Histopathological examination confirmed severe reactive fibroplasia and inflammation, surrounding sections of a nematode worm. The latter was then identified with polymerase chain reaction as belonging to the Cylicospirura genus. Cylicospirura species worms should be considered in the differential diagnosis of gastric nodules in the cat. © ISFM and AAFP 2013.

Dorbolo S.,University of Liège | Ludewig F.,University of Liège | Vandewalle N.,University of Liège | Laroche C.,Private Laboratory
Physical Review E - Statistical, Nonlinear, and Soft Matter Physics | Year: 2012

The melting of an assembly of ice blocks contained in a vertical cylinder and under an unidirectional load was investigated. The total volume occupied by the ice blocks and the volume of ice were simultaneously measured which allowed one to determine the volume fraction of the ice in the cylinder. While the ice volume continuously decreases, sudden breakdowns of the total volume were observed. Large reorganizations of the whole assembly occur. However, the maximal volume fraction found just after a large reorganization decreased with time. In addition, the modifications of the pile structure were investigated using an x-ray tomography imaging before and after one collapse. As the packing is better ordered along the walls, we suggest that the motion of the piston is governed by the layer of ice blocks located along the container wall. This layer was modeled by a two-dimensional assembly of disks. The model supports the idea that the geometrical frustrations explain the dynamics of the successive reorganization due to the shrinkage of the grains. Finally, numerical simulations allow one to conclude that the dynamics of the melting of the ice blocks is governed (i) by the confinement effect which induces defects in the packing and (ii) by the low friction between the ice blocks. © 2012 American Physical Society.

Dimitriadi D.,Private Laboratory
Central European Journal of Urology | Year: 2014

Introduction Oral sex (fellatio) is a very common sexual activity. H. pylori is mainly a gastric organism, but studies have reported that infected individuals may permanently or transiently carry H. pylori in their mouth and saliva.Material and methods A Pubmed search was conducted using the words infection, oral sex and urethritis.Results The existing studies support the hypothesis that H. pylori could be a causative agent of non-gonococcal urethritis.Conclusions It is possible that H. pylori may be transmitted via the act of fellatio in the urethra. Further research is required to explore the role of H. pylori in sexually transmitted urethritis. © 2014 Polish Urological Association. All rights reserved.

Costa C.,Italian Agricultural Research Council | Menesatti P.,Italian Agricultural Research Council | Rambaldi E.,Consorzio Mediterraneo | Argenti L.,Private laboratory | Bianchini M.L.,CNR Institute of Agro-environmental and Forest Biology
Aquacultural Engineering | Year: 2013

Product diversification, among which organic farming, is an important issue in modern aquaculture activities. Discriminating organic vs. conventional products is complex, but appearance may help in tracing different batches of produce. To test this fact, sea basses were fed a commercial or an organic diet, and fishes of each different group were photographed before and during the experiment. Body landmarks were digitized on each colour-calibrated (using the TPS-3D algorithm) image; on the basis of landmarks configuration, the RGB matrices were warped using a geometric morphometrics procedure. The calibrated colour matrix of each warped individual (195 × 135,225) was analyzed with a 50-50 MANOVA, followed by a partial least squares discriminant analysis. Finally, a cluster analysis on the diet/time groups was performed. Growth and changes in condition factor over time are not dependent on the rearing method. Colour (as represented by the pixel vector) does depend on time and on rearing method, based on the MANOVA method used. Standard length and condition factor were not good predictors of colour. The partial least square discriminant analysis was highly effective in detecting colour differences on the basis of the fish diet. The 9-group dendrogram showed that the wild sample and the organic fish cluster together. The head, darker in fishes raised conventionally, is the part showing the greatest difference; the longer the life spent under the 2 regimens, the stronger the differences. In conclusion, these preliminary results demonstrate that a colorimetric analysis is able to distinguish 2 batches of fishes fed different diets in different environmental conditions and - in the present instance - to certify the organically grown sea basses. © 2013 Elsevier B.V.

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