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Minsk, Belarus

Ryazantsev D.Y.,RAS Shemyakin Ovchinnikov Institute of Bioorganic Chemistry | Tsybulsky D.A.,Institute of Physical Organic Chemistry | Prokhorenko I.A.,RAS Shemyakin Ovchinnikov Institute of Bioorganic Chemistry | Kvach M.V.,Institute of Physical Organic Chemistry | And 5 more authors.
Analytical and Bioanalytical Chemistry | Year: 2012

A typical TaqMan™ real-time PCR probe contains a 5'-fluorescent dye and a 3'-quencher. In the course of the amplification, the probe is degraded starting from the 5'- end, thus releasing fluorescent dye. Some fluorophores (including fluorescein) are known to be prone to self-quenching when located near each other. This work is aimed at studying dye-dye and dye-quencher interactions in multiply modified DNA probes. Twenty-one fluorogenic probes containing one and two fluoresceins (FAM), or a FAM-JOE pair, and one or two BHQ1 quenchers were synthesized using non-nucleoside reagents and "click chemistry" post-modification on solid phase and in solution. The probes were tested in real-time PCR using an ~300-bp-long natural DNA fragment as a template. The structural prerequisites for lowering the probe background fluorescence and increasing the end-plateau fluorescence intensity were evaluated and discussed. © Springer-Verlag 2012. Source

Tan T.,Primetech LLC | Watts S.W.,Michigan State University | Davis R.P.,Michigan State University
Frontiers in Pharmacology | Year: 2011

Successful drug delivery using implantable pumps may be found in over 12,500 published articles. Their versatility in delivering continuous infusion, intermittent or complex infusion protocols acutely or chronically has made them ubiquitous in drug discovery and basic research. The recent availability of iPRECIO®, a programmable, refillable, and implantable infusion pump has made it possible to carry out quantitative pharmacology (PKPD) in single animals. When combined with specialized catheters, specific administration sites have been selected. When combined with radiotelemetry, the physiologic gold standard, more sensitive and powerful means of detecting drug induced therapeutic, and/or adverse effects has been possible. Numerous application examples are cited from iPRECIO® use in Japan, United States, and Europe with iPRECIO® as an enabling drug delivery device where the refillable and programmability functionality were key benefits. The ability to start/stop drug delivery and to have control periods prior dosing made it possible to have equivalent effects at a much lower dose than previously studied. Five different iPRECIO® applications are described in detail with references to the original work where the implantable, refillable, and programmable benefits are demonstrated with their different end-points. © 2011 Tan, Watts and Davis. Source

Ryazantsev D.Y.,RAS Shemyakin Ovchinnikov Institute of Bioorganic Chemistry | Kvach M.V.,Institute of Physical Organic Chemistry | Tsybulsky D.A.,Institute of Physical Organic Chemistry | Prokhorenko I.A.,RAS Gause Institute of New Antibiotics | And 6 more authors.
Analyst | Year: 2014

Convenient preparation of fluorogenic hairpin DNA probes (molecular beacons) carrying a pair of FAM fluorophores (located close to 5′-terminus of the probe) or a pair of BHQ1 quenchers on 3′-terminus (with (BHQ1)2 or BHQ1-BHQ1 composition) is reported. These probes were used for the first time in a real-time PCR assay and showed considerable improvements in fluorogenic properties (the total fluorescence increase or signal-to-background ratio) in assay conditions vs. conventional one-FAM-one-BHQ1 molecular beacon probes as well as vs. hydrolyzable one-FAM-one-BHQ1 TaqMan probes. At the same time, such multiple modifications of the probe do not influence its Cq (a fractional PCR cycle used for quantification). The probe MB14 containing a BHQ1-BHQ1 pair showed a PCR fluorescence/background value of 9.6 which is more than two times higher than that of a regular probe MB2 (4.6). This study demonstrates prospects for the design of highly fluorogenic molecular beacon probes suitable for quantitative real-time PCR and for other potential applications (e.g. intracellular RNA detection and SNP/mutation analysis). © the Partner Organisations 2014. Source

Primetech Corporation | Date: 2015-06-17

medical apparatus, namely, implantable micro infusion pumps and not implantable programmable infusion pumps for use in laboratory animals.

Primetech Co. | Date: 2013-05-17

Disclosed herein is a test socket for camera modules, which enables automated inspection of camera modules. The test socket for camera modules includes a base plate provided with a loading unit on which a camera module to be tested is placed; a slider formed on the base plate and horizontally movable thereon; an upper plate formed on the slider to move up or down thereon, and including a cover corresponding to an upper portion of the camera module; a vertical cylinder allowing the upper plate to vertically reciprocate with respect to the slider; and a pin block formed on the loading unit or the cover and connected to a contact point of the camera module.

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