News Article | May 18, 2017
WAYNE, PA, May 18, 2017-- Prime Technology Group, LLC, a leading provider of innovative technology solutions and services, today announced the release of Xemplar Fleet 2.0. As a carrier solution, Xemplar adds a new dimension to commercial auto products with a vast range of features and services focused on enhanced management of commercial vehicles and drivers, all wrapped around a robust telematics engine. Xemplar Fleet provides commercial drivers, fleet managers, and carriers the critical data to make better business decisions."Xemplar Fleet has been carefully designed to address loss ratio issues in Commercial Auto books, and improve customer engagement by providing a B2B solution to business owners and enterprise-level Fleet Managers with the tools to lower their risk exposures," says SK Tirumala, Executive Vice President of Insurance Solutions at Prime. "Safe driving on the roads is immediately needed across all types of vehicles and drivers to curb growing losses caused by distracted driving and ever-increasing traffic conditions. Xemplar directly addresses these challenges with its innovative approach."Built to generate real value and tangible benefits, Xemplar leverages the power of analytics on its existing telematics capabilities to significantly simplify, accelerate and impact the way carriers engage with policyholders. The new solution blends risk detection and prevention, customer service and marketing elements into a single channel - making it a must-have solution for commercial auto insurers.Xemplar Fleet is a co-branded, cloud-based, mobile solution that is easy to install, and can be tailored to suit the needs of any carrier. It enables a new communication channel to reduce and manage risk factors both proactively and reactively. Emphasizing the need for proactive prioritization of risk management strategies, SK added, "Insurers need not worry about policyholder's adoption of innovative risk management strategies. These solutions may appear intrusive initially, but in fact, people are more willing, than one would think, to trade data for increased safety, greater service, and reduce costs".Talking about the recent back-to-back launches of Xemplar solutions for Commercial and Personal Auto Insurers, Sudhakar Goverdhanam, Chief Executive Officer of Prime Technology Group, said, "We envisioned a solution that could significantly bring together carriers and policyholders and empower them to promote road safety by proactively managing risks. I am excited about this launch as it is in tune with Prime's goal of bringing compelling solutions to the market that help businesses thrive in these unpredictable times."Headquartered in suburban Philadelphia, Pennsylvania (U.S.), Prime Technology Group is a highly-diversified IT solutions company that combines innovation, deep industry expertise and a passion for client success. Founded in the year 1999, Prime serves the global markets in Healthcare, Insurance, Finance, and IT Consulting services with a collaborative workforce of more than 700 employees worldwide.The breadth and scope of Prime's global enterprise consistently strives to ensure efficient current operations and cost-effective business processes to unleash new opportunities for growth.Visit our website xemplartelematics.com to learn more about what's new and request a demo.Contact:Prime Technology Group610-205-8740Source:
Takeda K.,Japan National Agriculture and Food Research Organization |
Tasai M.,Japan National Agriculture and Food Research Organization |
Akagi S.,Japan National Agriculture and Food Research Organization |
Matsukawa K.,Japan National Agriculture and Food Research Organization |
And 8 more authors.
Mitochondrion | Year: 2010
Microinjection of isolated mitochondria into oocytes is an effective method to introduce exogenous mitochondrial DNA. In nuclear transfer procedures in which donor cell mitochondria are transferred with nuclei into recipient oocytes; development and survival rates of reconstructed embryos may be also directly influenced by mitochondrial viability. Mitochondrial viability is dramatically affected by cell culture conditions, such as serum starvation prior to nuclear transfer. This study was conducted to examine the influence of exogenous mitochondria using bovine and mouse parthenogenetic models. Mitochondria were isolated from primary cells at confluency and after serum starvation. The bovine oocytes injected with serum-starved mitochondria showed lower rates of morula and blastocyst formation when compared to uninjected controls (P < 0.05). However, the developmental rates between non-starved mitochondria injection and controls were not different (P > 0.05). The murine oocytes injected with serum-starved mitochondria showed lower rates of development when compared with non-starved mitochondria and controls (P < 0.01). In contrast to mitochondria transfer, ooplasm transfer did not affect murine or bovine parthenogenetic development (P > 0.05). The overall results showed that injection of serum-starved mitochondria influenced parthenogenetic development of both bovine and murine oocytes. Our results illustrate that the somatic mitochondria introduction accompanying nuclei has the capacity to affect reconstructed embryo development; particularly when using serum-starved cells as donor cells. © 2009 Mitochondria Research Society.
PubMed | Japan National Institute of Agrobiological Science, Tohoku University, Prime Technology Ltd and Nihon University
Type: Journal Article | Journal: Journal of the American Heart Association | Year: 2016
Although clinical trials have proved that statin can be used prophylactically against cardiovascular events, the direct effects of statin on plaque development are not well understood. We generated low-density lipoprotein receptor knockout (LDLR(-/-)) pigs to study the effects of early statin administration on development of atherosclerotic plaques, especially advanced plaques.LDLR(-/-) pigs were generated by targeted deletion of exon 4 of the LDLR gene. Given a standard chow diet, LDLR(-/-) pigs showed atherosclerotic lesions starting at 6 months of age. When 3-month-old LDLR(-/-) pigs were fed a high-cholesterol, high-fat (HCHF) diet for 4 months (HCHF group), human-like advanced coronary plaques developed. We also fed 3-month-old LDLR(-/-) pigs an HCHF diet with pitavastatin for 4 months (Statin Prophylaxis Group). Although serum cholesterol concentrations did not differ significantly between the 2 groups, intravascular ultrasound revealed 52% reduced plaque volume in statin-treated pigs. Pathological examination revealed most lesions (87%) in the statin prophylaxis group were early-stage lesions, versus 45% in the HCHF diet group (P<0.01). Thin-cap fibroatheroma characterized 40% of the plaques in the HCHF diet group versus 8% in the statin prophylaxis group (P<0.01), intraplaque hemorrhage characterized 11% versus 1% (P<0.01), and calcification characterized 22% versus 1% (P<0.01).Results of our large animal experiment support statin prophylaxis before the occurrence of atherosclerosis. Early statin treatment appears to retard development of coronary artery atherosclerosis and ensure lesion stability. In addition, the LDLR(-/-) pigs we developed represent a large animal model of human-like advanced coronary plaque suitable for translational research.
Iwasaki K.,Nagoya University |
Miwa Y.,Nagoya University |
Ogawa H.,Obihiro University of Agriculture and Veterinary Medicine |
Yazaki S.,Prime Technology Ltd. |
And 10 more authors.
Transplantation | Year: 2012
Background.: Recent development of immunosuppressive therapy has provided a platform for clinical human leukocyte antigen (HLA)-and ABO-incompatible kidney transplantation. However, the prognosis seems to be different between the two. Accommodation, the condition of no injury even in the presence of antidonor antibody, is one of the key factors for successful transplantation with antidonor antibody. The purpose of this study was to compare signal transduction between anti-A/B and anti-HLA antibody reaction and to elucidate the mechanisms underlying accommodation. Methods.: Blood type A-or B-transferase gene was transfected into human EA.hy926 endothelial cells. After cell sorting, A-or B-expressing cells at high levels were obtained. The effects of anti-HLA and anti-A/B antibody binding on complement-mediated cytotoxicity and signal transduction were examined. Results.: Preincubation with anti-HLA antibodies only at low levels (<10% of saturation level) or anti-A/B antibodies at high levels (even at near saturation levels) for 24 hr resulted in resistance to complement-mediated cytotoxicity. Anti-A/B antibody ligation inactivated ERK1/2 pathway and increased complement regulatory proteins such as CD55 and CD59, whereas anti-HLA ligation activated PI3K/AKT pathway and increased cytoprotective genes such as hemeoxygenase-1 and ferritin H. Conclusion.: Complement inhibition by upregulation of CD55 and CD59 through ERK1/2 inactivation might play a substantial role in accommodation after ABO-incompatible transplantation, which could also explain the intriguing finding of C4d deposition in the graft without rejection. © 2012 by Lippincott Williams & Wilkins.
Sembon S.,Japan National Institute of Agrobiological Science |
Fuchimoto D.,Japan National Institute of Agrobiological Science |
Iwamoto M.,Prime Technology Ltd. |
Suzuki S.,Japan National Institute of Agrobiological Science |
And 2 more authors.
Theriogenology | Year: 2011
The objective was to produce porcine tetraploid parthenogenetic embryos using cytochalasin B, which inhibits polar body extrusion. Porcine cumulus-enclosed oocytes aspirated from antral follicles were cultured for 51 h, and treated with cytochalasin B from 35 h to 42 h after the start of culture. After maturation culture, 74.7% (2074/2775) of oocytes treated with cytochalasin B did not extrude a polar body (0PB oocytes). In contrast, 80.4% (1931/2403) of control oocytes extruded a polar body (1PB oocytes). The 0PB oocytes were electrically stimulated, treated with cytochalasin B again for 3 h, and then cultured without cytochalasin B. Six days after electrical stimulation, 49.8% (321/644) reached the blastocyst stage. The number of cells in these blastocysts derived from 0PB oocytes was significantly lower than that from 1PB oocytes (0PB: 24.9 10.6; 1PB: 43.0 17.1; mean SD). A porcine chromosome 1-specific sequence was detected in parthenogenetic 0PB embryos by fluorescence in situ hybridization (FISH) analysis. Typical pronucleus-stage samples derived from 0PB embryos had two pronuclei, each with two signals. In two-cell and blastocyst-stage embryos, four signals were detected in each nucleus derived from 0PB embryos. We inferred that 0PB oocytes, which had a tetraploid number of chromosomes, started to develop as tetraploid parthenotes after electrical stimulation, and that tetraploid status was stably maintained during early embryonic development, at least until the blastocyst stage. © 2011 Elsevier Inc.
Miwa Y.,Nagoya University |
Yazaki S.,Prime Technology Ltd |
Iwamoto M.,Prime Technology Ltd |
Suzuki S.,Japan National Institute of Agrobiological Science |
And 7 more authors.
Transplantation | Year: 2015
For successful xenotransplantation, in addition to á1,3-galactosyltransferase gene-knockout and human complement regulatory protein (CD46, CD55, CD59) gene insertion, cloned pigs expressing human thrombomodulin (hTM) have been produced to solve the problem of molecular incompatibility in their coagulation system. Recombinant soluble hTM (S-hTM) which has been recently approved for treatment of disseminated intravascular coagulation might be potentially available. The purpose of this study is to examine the functional difference in endothelial cells between membrane-bound hTM (MB-hTM) and S-hTM and to elucidate effective strategy using both types of hTM. Methods. The following factors regarding coagulation and inflammation were compared between hTM-expressing pig aortic endothelial cells (PAEC) derived from cloned pig and nontransgenic PAEC in the presence of S-hTM under tumor necrosis factor-á-activated conditions; (i) clotting time (ii) pig tissue factor (TF), (iii) pig Eselectin, (iv) direct prothrombinase activity, (v) activated protein C (APC), and (vi) prothrombinase activity. Results. The MBhTM significantly suppressed the expression of pig TF and E-selectin and direct prothrombinase activity in tumor necrosis factor-α-activated PAEC, suggesting strong anti-inflammatory effect, compared to S-hTM. In contrast, S-hTM had more potent capacity to inhibit thrombin generation and to produce APC than MB-hTM, although MB-hTM had the same level of capacity as human endothelial cells. Conclusions. It was speculated that S-hTM treatment would be of assistance during high-risk periods for excessive thrombin formation (e.g., ischemia reperfusion injury or severe infection/rejection). Considering the properties of MB-hTM exhibiting anti-inflammatory function as well as APC production, hTM-expressing cloned pigs might be indispensible to long-term stabilization of graft endothelial cells. © Wolters Kluwer Health, Inc.
Sembon S.,Japan National Institute of Agrobiological Science |
Iwamoto M.,Japan National Institute of Agrobiological Science |
Hashimoto M.,Japan National Institute of Agrobiological Science |
Oishi T.,Japan National Institute of Agrobiological Science |
And 4 more authors.
Theriogenology | Year: 2012
In livestock, parthenogenic embryos are simple to produce, but androgenetic embryos have been successfully produced only in sheep and cows. In the present study, matured porcine oocytes were enucleated by micromanipulation and then fertilized with sperm in vitro, thereby producing porcine androgenetic embryos. Porcine androgenetic embryos, which had only sperm genomes, were assessed for cleavage and for blastocyst formation 2 and 6 d after IVF, respectively. There was no difference in cleavage rate between androgenetic embryos and biparental IVF embryos (mean ± SD androgenetic: 65.5 ± 5.4%; biparental IVF: 63.2 ± 3.6%), but there was a difference in the rate of blastocyst formation (androgenetic: 4.5 ± 0.7%; biparental IVF: 30.2 ± 2.6%, P < 0.05). The average number of cells in Day 6 androgenetic blastocysts (34.3 ± 18.2) was lower (P < 0.05) than that in biparental IVF blastocysts (44.1 ± 19.5), but did not differ from that in parthenogenetic embryos (35.7 ± 16.7). The androgenetic embryos were transferred into recipient mothers to examine the competence of post-implantation development. Androgenetic fetuses were present on Days 21 and 25, but not on Days 28, 31, or 35. Of the six androgenetic fetuses recovered on Day 21, five had normal, translucent bodies, and two of these five had beating hearts. The four fetuses recovered on Day 25 were all non-viable. In conclusion, porcine androgenetic embryos initiated embryogenesis and had reached a viable fetal stage 21 days after IVF. © 2012 Elsevier Inc.
Prime Technology LLC | Date: 2011-04-19
Pressure sensors; pressure transmitters; level indicators; temperature indicators; panel meters, namely, an electronic instrument that displays an input signal in digital form; temperature sensors; liquid level and flow indicators.
PubMed | Japan National Institute of Agrobiological Science and Prime Technology Ltd.
Type: | Journal: Veterinary immunology and immunopathology | Year: 2016
Pigs with severe combined immunodeficiency (SCID) are versatile animal models for human medical research because of their biological similarities to humans, suitable body size, and longevity for practical research. SCID pigs with defined mutation(s) can be an invaluable tool for research on porcine immunity. In this study, we produced RAG2-knockout pigs via somatic cell nuclear transfer and analyzed their phenotype. The V(D)J recombination processes were confirmed as being inactivated. They consistently lacked mature T and B cells but had substantial numbers of cells considered to be T- or B-cell progenitors as well as NK cells. They also lacked thymic medulla and lymphoid aggregations in the spleen, mesenteric lymph nodes, and ileal Peyers patches. We showed more severe immunological defects in the RAG2 and IL2RG double-knockout pig through this study. Thus, SCID pigs could be promising animal models not only for translational medical research but also for immunological studies of pigs themselves.
PubMed | Prime Technology Ltd
Type: Journal Article | Journal: Xenotransplantation | Year: 2012
For long-term xenograft survival, coagulation control is one of the remaining critical issues. Our attention has been directed toward human thrombomodulin (hTM), because it is expected to exhibit the following beneficial effects on coagulation control and cytoprotection: (i) to solve the problem of molecular incompatibility in protein C activation; (ii) to exert a role as a physiological regulator, only when thrombin is formed; (iii) to suppress direct prothrombinase activity; and (iv) to have anti-inflammatory properties. hTM gene was transfected into pig (Landrace/Yorkshire) fibroblasts using pCAGGS expression vector and pPGK-puro vector. After puromycin selection, only fibroblasts expressing a high level of hTM were collected by cell sorting and then applied to nuclear transfer. Following electroactivation and subsequent culture, a total of 1547 cleaved embryos were transferred to seven surrogate mother pigs. Two healthy cloned piglets expressing hTM were born, successfully grew to maturity and produced normal progeny. Immunohistochemical staining of organs from F1 generation pigs demonstrated hTM expression in endothelial cells as well as parenchymal cells. High expression was observed particularly in endothelial cells of kidney and liver. Aortic endothelial cells from cloned pigs were found to express hTM levels similar to human umbilical vein endothelial cells (HUVEC) and to make it possible to convert protein C into activated protein C. The blockade of human endothelial cell protein C receptor (hEPCR) significantly reduced APC production in HUVEC, but not in hTM-PAEC. Although no bleeding tendency was observed in hTM-cloned pigs, activated partial thromboplastin time (APTT) was slightly prolonged and soluble hTM was detected in pig plasma. hTM was expressed in platelets and mononuclear cells, but not in RBC. Cloned pigs expressing hTM in endothelial cells at a comparable level to HUVEC were produced. As complete suppression of antigen-antibody reaction in the graft is essential for accurate assessment of transgene related to coagulation control, production of genetically engineered pigs expressing hTM and complement regulatory protein based on galactosyltransferase knockout is desired.