Rhodin A.,Uppsala University Hospital |
Rhodin A.,Central Hospital |
Gronbladh A.,Uppsala University |
Ginya H.,Precision System Science Co |
And 8 more authors.
Molecular Brain | Year: 2013
Background: Opioids are associated with wide inter-individual variability in the analgesic response and a narrow therapeutic index. This may be partly explained by the presence of single nucleotide polymorphisms (SNPs) in genes encoding molecular entities involved in opioid metabolism and receptor activation. This paper describes the investigation of SNPs in three genes that have a functional impact on the opioid response: OPRM1, which codes for the μ-opioid receptor; ABCB1 for the ATP-binding cassette B1 transporter enzyme; and the calcium channel complex subunit CACNA2D2. The genotyping was combined with an analysis of plasma levels of the opioid peptide β-endorphin in 80 well-defined patients with chronic low back pain scheduled for spinal fusion surgery, and with differential sensitivity to the opioid analgesic remifentanil. This patient group was compared with 56 healthy controls. Results: The plasma β-endorphin levels were significantly higher in controls than in pain patients.A higher incidence of opioid-related side effects and sex differences was found in patients with the minor allele of the ABCB1 gene. Further, a correlation between increased opioid sensitivity and the major CACNA2D2 allele was confirmed. A tendency of a relationship between opioid sensitivity and the minor allele of OPRM1 was also found. Conclusions: Although the sample cohort in this study was limited to 80 patients it appears that it was possible to observe significant correlations between polymorphism in relevant genes and various items related to pain sensitivity and opioid response. Of particular interest is the new finding of a correlation between increased opioid sensitivity and the major CACNA2D2 allele. These observations may open for improved strategies in the clinical treatment of chronic pain with opioids. © 2013 Rhodin et al.; licensee BioMed Central Ltd.
Kalliomaki M.-L.,Uppsala University |
Kalliomaki M.-L.,University of Tampere |
Sandblom G.,Karolinska Institutet |
Hallberg M.,Uppsala University |
And 6 more authors.
Scandinavian Journal of Pain | Year: 2016
Background and aims: Despite improvements in surgical technique, 5%-8% of patients undergoing herniorrhaphy still suffer from clinically relevant persistent postherniotomy pain. This is a problem at both individual and society levels. The aim of this study was to determine whether or not a single nucleotide polymorphism in a specific gene contributes to the development of persistent pain after surgery. Methods: One hundred individuals with persistent postherniotomy pain, along with 100 without pain matched for age, gender and type of surgery were identified in a previous cohort study on patients operated for groin hernia. All patients underwent a thorough sensory examination and blood samples were collected. DNA was extracted and analysed for single nucleotide polymorphism in the Mu opioid receptor, TNF-α, GRIK3, GCH1, BDNF and CACNA2D2 genes. Results: Patients with neuropathic pain were found to have a homozygous single nucleotide polymorph in the TNF-α gene significantly more often than pain-free patients (P = 0.036, one-tailed test). Conclusions: SNP in the TNF-α gene has a significant impact on the risk for developing PPSP. Implications: The result suggests the involvement of genetic variance in the development of pain and this requires further investigation. © 2015 Scandinavian Association for the Study of Pain.
Mitarai S.,Research Institute of Tuberculosis |
Karinaga R.,Genetein Co. |
Karinaga R.,Precision System Science Co. |
Yamada H.,Research Institute of Tuberculosis |
And 6 more authors.
Journal of Microbiological Methods | Year: 2012
Centrifugation is a necessary concentrating step for the detection of Mycobacterium tuberculosis in a liquid culture. However, centrifugation is biologically hazardous and presents an obstacle in the development of an automated culture system. A bead-based bacterial concentration method, TRICORE, was recently developed by Genetein Co., Ltd. We compared the efficacy of TRICORE and conventional centrifugation for concentrating M. tuberculosis in clinical sputum specimens by using liquid and solid culture systems. Among 90 pretreated clinical sputum specimens, 51 (57.3%) and 55 (61.8%) M. tuberculosis isolates were recovered by the MGIT culture system by using the centrifugation and TRICORE methods, respectively (chi-square test, p=. 0.5413). The detection time for the centrifugation method was 359.3 ± 117.0. h, while that for the bead-based concentration method was 377.6 ± 162.3. h (p=. 0.5637). However, the number of colonies recovered on solid media were significantly higher with the TRICORE method (p=. 0.003). In particular, among the smear-negative specimens, culture positivity of the TRICORE method was 39.6%, while that of the centrifugation method was 15.1%. The TRICORE bead-based concentration method was considered equivalent to centrifugation and enabled efficient collection of paucibacillary specimens in solution. Thus, the new noncentrifugation concentration method could yield more positive culture results. © 2012 Elsevier B.V..
Ginya H.,Tokyo University of Agriculture and Technology |
Ginya H.,Precision System Science Co. |
Matsushita R.,Tokyo University of Agriculture and Technology |
Yohda M.,Tokyo University of Agriculture and Technology
Journal of Bioscience and Bioengineering | Year: 2010
We estimated the actual error rate during ligase detection reaction (LDR), and confirmed that DNA sequences around 3′ ends are greatly influenced on the specificity of LDR. Its specificity is increased about 1000 times by introducing a mismatch base near the 3′ ends. © 2009 The Society for Biotechnology, Japan.
Precision System Science Co. | Date: 2010-12-08
It is an object of the present invention to provide a container which can execute a reliable work of dispensing and/or agitating on the occasion of aspirating and/or discharging a sample and/or a reagent in a container, by aspirating or discharging in a predetermined quantity without blockading the front end portion of the liquid sucking/discharging line. It is another object of the present invention to provide a container having a simple structure which can realize a high precision inspection for all of the quantity by aspirating all of the quantity in the container, by being capable of discharging all of the quantity in the container without necessity of the surplus of the samples and/or reagents and by treating all of the quantity as a fixed quantity. The present invention is a container comprising a gap part formed to have a smaller width than a caliber of a front end portion of a liquid sucking/discharging line adapted to be inserted therein and pulled out thereof, at an inside bottom part thereof. The gap part is formed to be a shape being capable of aspirating and discharging all of the quantity of liquid through the front end portion even in a state that the front end portion is disposed in contact with the inside bottom part. On the occasion of discharging, the container is formed so that reaction between a sample and a reagent can be uniformed by discharging and diffusing aspirated liquid.
Precision System Science Co. | Date: 2011-01-19
A container has a base member, and plural vessels arranged along a line or lines in the base member, wherein the plural vessels comprise the necessary number of vessels for treatment, vessels for measuring light being able to couple with an optical measuring apparatus or an optical receiving unit in a light shielded state, or hole parts holding it, vessels accommodating or hole parts holding a pipette tip, tubes for PCR or hole parts holding it, or, vessels being contained in the solid phase or hole parts holding it, according to the process. Thus, the process from beginning to the optical measurement can be completed by only one container.
Precision System Science Co. | Date: 2010-03-30
Chemical carriers having oligo-nucleotides bonded therewith for analysis of DNA sequences, not for medical or veterinary use; Kit comprised of chemical carriers having oligo-nucleotides bonded herewith for analysis of DNA sequences, chemicals, and chemical test paper for analysis of biochemical and biological substances, namely, DNA, RNA, oligo-nucleotides, nucleotides, polynucleotides, proteins, carbohydrates, antibodies, antigens, and high molecular weight compounds, not for medical or veterinary use; Chemical test paper. Laboratory apparatus and instruments, namely, flow cell meters, pipette, pipette tips, capillary tubes for laboratory use, test tubes, microtubes, culture trays, seals for laboratory bottles, reagent reservoirs, disposable reusable dispenser syringes for laboratory use, graduated dispensers, and solid and liquid waste receptacles, all for use in the analysis, testing, and research of biochemical and biological substances, namely, DNA, RNA, oligo-nucleotides, nucleotides, poly-nucleotides, proteins, carbohydrates, antibodies, antigens, and high molecular weight compounds; Measuring and testing machines and instruments for analysis of biochemical and biological substances, namely, DNA sequencers, genetic analyzers, fragment analyzers, electrophoresis machines, linkage analyzers, genetic mappers, organic and inorganic synthesizers, mass spectrometers, spectrophotometers, meters for measuring luminescence absorbency, fluorescence, and time-resolved fluorescence, fluorescent scanners, liquid chromatographs, thermometers not for medical use, and chronographs for use as specialized time recording apparatus; Downloadable electronic publications in the nature of instructions and manuals in the fields of laboratory apparatus and instruments, measuring or testing machines and instruments, medical machines and apparatus all for analysis of biochemical and biological substances.
Precision System Science Co. | Date: 2011-01-28
Laboratory apparatus and instruments for making analysis for biochemical or biological substances, such as DNA, RNA, oligo-nucleotides, nucleotides and poly-nucleotides, namely, reagent delivering devices, pipette devices, magnetic means built-in pipette devices, pipette tips, vessels, linear cartridge vessels, and robotic workstations for automatic extraction, amplification (PCR), or detection of genetic material from biological samples; measuring or testing machines and instruments for analysis of biochemical and biological substances, namely, DNA sequencers, genetic analyzers, fragment analyzers, linkage analyzers, genetic mappers, fluorescent scanners, incubators, immunoassay analyzers, and automated workstations comprised of laboratory robots, computers and computer monitors.
Precision System Science Co. | Date: 2011-08-30
Laboratory apparatus and instruments, namely, pipettes, pipette tips, capillaries, reagent reservoirs, syringes, solid and liquid waste receptacles, all for use in the analysis, testing, and research of biochemical and biological substances, namely, DNA, RNA, oligo-nucleotides, nucleotides, poly-nucleotides, proteins, carbohydrates, antibodies, antigens, and high molecular weight compounds; measuring or testing machines and instruments, namely, DNA sequencers, genetic analyzers, fragment analyzers, electrophoresis machines, linkage analyzers, genetic mappers, organic and inorganic synthesizers, robotic workstations comprised of laboratory robots, computers and computer monitors, incubators, immunoassay analyzers, sample preparation and screening workstations comprised of containers for biological substances and liquid magnetic particle suspensions, distributors for applying and removing magnetic particles bound to biological substances; downloadable electronic publications in the nature of manuals for use in the operation of electronic laboratory equipment for measuring.
PRECISION SYSTEM SCIENCE Co. | Date: 2010-10-12
Medical diagnostic reagents, namely, reagents for extracting nucleic acid.