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Kalliomaki M.-L.,Uppsala University | Kalliomaki M.-L.,University of Tampere | Sandblom G.,Karolinska Institutet | Hallberg M.,Uppsala University | And 6 more authors.
Scandinavian Journal of Pain | Year: 2016

Background and aims: Despite improvements in surgical technique, 5%-8% of patients undergoing herniorrhaphy still suffer from clinically relevant persistent postherniotomy pain. This is a problem at both individual and society levels. The aim of this study was to determine whether or not a single nucleotide polymorphism in a specific gene contributes to the development of persistent pain after surgery. Methods: One hundred individuals with persistent postherniotomy pain, along with 100 without pain matched for age, gender and type of surgery were identified in a previous cohort study on patients operated for groin hernia. All patients underwent a thorough sensory examination and blood samples were collected. DNA was extracted and analysed for single nucleotide polymorphism in the Mu opioid receptor, TNF-α, GRIK3, GCH1, BDNF and CACNA2D2 genes. Results: Patients with neuropathic pain were found to have a homozygous single nucleotide polymorph in the TNF-α gene significantly more often than pain-free patients (P = 0.036, one-tailed test). Conclusions: SNP in the TNF-α gene has a significant impact on the risk for developing PPSP. Implications: The result suggests the involvement of genetic variance in the development of pain and this requires further investigation. © 2015 Scandinavian Association for the Study of Pain.


Rhodin A.,Uppsala University Hospital | Rhodin A.,Center for Clinical Research | Gronbladh A.,Uppsala University | Ginya H.,Precision System Science Co. | And 8 more authors.
Molecular Brain | Year: 2013

Background: Opioids are associated with wide inter-individual variability in the analgesic response and a narrow therapeutic index. This may be partly explained by the presence of single nucleotide polymorphisms (SNPs) in genes encoding molecular entities involved in opioid metabolism and receptor activation. This paper describes the investigation of SNPs in three genes that have a functional impact on the opioid response: OPRM1, which codes for the μ-opioid receptor; ABCB1 for the ATP-binding cassette B1 transporter enzyme; and the calcium channel complex subunit CACNA2D2. The genotyping was combined with an analysis of plasma levels of the opioid peptide β-endorphin in 80 well-defined patients with chronic low back pain scheduled for spinal fusion surgery, and with differential sensitivity to the opioid analgesic remifentanil. This patient group was compared with 56 healthy controls. Results: The plasma β-endorphin levels were significantly higher in controls than in pain patients.A higher incidence of opioid-related side effects and sex differences was found in patients with the minor allele of the ABCB1 gene. Further, a correlation between increased opioid sensitivity and the major CACNA2D2 allele was confirmed. A tendency of a relationship between opioid sensitivity and the minor allele of OPRM1 was also found. Conclusions: Although the sample cohort in this study was limited to 80 patients it appears that it was possible to observe significant correlations between polymorphism in relevant genes and various items related to pain sensitivity and opioid response. Of particular interest is the new finding of a correlation between increased opioid sensitivity and the major CACNA2D2 allele. These observations may open for improved strategies in the clinical treatment of chronic pain with opioids. © 2013 Rhodin et al.; licensee BioMed Central Ltd.


Ginya H.,Tokyo University of Agriculture and Technology | Ginya H.,Precision System Science Co. | Matsushita R.,Tokyo University of Agriculture and Technology | Yohda M.,Tokyo University of Agriculture and Technology
Journal of Bioscience and Bioengineering | Year: 2010

We estimated the actual error rate during ligase detection reaction (LDR), and confirmed that DNA sequences around 3′ ends are greatly influenced on the specificity of LDR. Its specificity is increased about 1000 times by introducing a mismatch base near the 3′ ends. © 2009 The Society for Biotechnology, Japan.


Trademark
Precision System Science Co. | Date: 2010-03-30

Chemical carriers having oligo-nucleotides bonded therewith for analysis of DNA sequences, not for medical or veterinary use; Kit comprised of chemical carriers having oligo-nucleotides bonded herewith for analysis of DNA sequences, chemicals, and chemical test paper for analysis of biochemical and biological substances, namely, DNA, RNA, oligo-nucleotides, nucleotides, polynucleotides, proteins, carbohydrates, antibodies, antigens, and high molecular weight compounds, not for medical or veterinary use; Chemical test paper. Laboratory apparatus and instruments, namely, flow cell meters, pipette, pipette tips, capillary tubes for laboratory use, test tubes, microtubes, culture trays, seals for laboratory bottles, reagent reservoirs, disposable reusable dispenser syringes for laboratory use, graduated dispensers, and solid and liquid waste receptacles, all for use in the analysis, testing, and research of biochemical and biological substances, namely, DNA, RNA, oligo-nucleotides, nucleotides, poly-nucleotides, proteins, carbohydrates, antibodies, antigens, and high molecular weight compounds; Measuring and testing machines and instruments for analysis of biochemical and biological substances, namely, DNA sequencers, genetic analyzers, fragment analyzers, electrophoresis machines, linkage analyzers, genetic mappers, organic and inorganic synthesizers, mass spectrometers, spectrophotometers, meters for measuring luminescence absorbency, fluorescence, and time-resolved fluorescence, fluorescent scanners, liquid chromatographs, thermometers not for medical use, and chronographs for use as specialized time recording apparatus; Downloadable electronic publications in the nature of instructions and manuals in the fields of laboratory apparatus and instruments, measuring or testing machines and instruments, medical machines and apparatus all for analysis of biochemical and biological substances.


Trademark
Precision System Science Co. | Date: 2009-04-07

Chemical carriers having oligo-nucleotides bonded therewith for analysis of DNA or RNA sequences, not for medical or veterinary use; Kit comprised of chemical carriers having oligo-nucleotides bonded herewith for analysis of DNA sequences, other Chemicals such as magnetic particles, enzymes, fluorescent materials, luminescent materials, chemi luminescent materials, and reagent for biochemical reaction (not for medical or veterinary use), Chemical test carriers for analysis of biochemical and biological substances, namely, DNA, RNA, Oligo-nucleotides, Nucleotide, Poly-nucleotides, Proteins, Amino acids, Carbohydrates, Antibodies, Antigens, and other high molecular weight compounds, not for medical or veterinary use. Laboratory apparatus and instruments, such as Pipette devices; Pipette tips; Capillaries; Test tubes; Microtubes; Trays; flasks; Seals; Reagent reservoirs; Syringes; Reagent Delivering devices; Magnetic means built-in Pipette devices; Vessels; Linear cartridge vessels, and Robotic work stations for automatic extraction, amplification (PCR), or detection of genetic material from biological samples, for making experiments, analysis, testing, and research for biochemical or biological substance, such as DNA, RNA, Oligo-nucleotides, Nucleotides, Poly-nucleotides, Proteins, Amino acids, Carbohydrates, Antibodies, Antigens, and other high molecular weight compounds, Measuring or testing machines and instruments for analysis of biochemical and biological substances, such as DNA sequencers, Genetic analyzers, Fragment analyzers; Linkage analyzers; Genetic mappers; Fluorescent scanners; Incubators, Immunoassay analyzers; Automated workstations comprised of laboratory robots, computers and computer monitors, Electronic machines, apparatus, and their parts, namely, central processing units, computer hardware, and computer software, all used in the testing, analysis, and examination of DNA, RNA, Oligo-nucleotides, Nucleotides, Poly-nuclcotides, Proteins, Amino acids, Carbohydrates, Antibodies, Antigens, and other high molecular weight compounds, Downloadable electronic publications in the nature of instructions or manuals in the field of laboratory apparatus and instruments, measuring or testing machines and instruments, medical machines and apparatus or biotechnology.

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