Walsh C.,University of British Columbia |
Ou K.,University of British Columbia |
Belliveau N.M.,Precision NanoSystems Inc. |
Leaver T.J.,Precision NanoSystems Inc. |
And 13 more authors.
Methods in Molecular Biology | Year: 2014
A simple, efficient, and scalable manufacturing technique is required for developing siRNA-lipid nanoparticles (siRNA-LNP) for therapeutic applications. In this chapter we describe a novel microfluidic-based manufacturing process for the rapid manufacture of siRNA-LNP, together with protocols for characterizing the size, polydispersity, RNA encapsulation efficiency, RNA concentration, and total lipid concentration of the resultant nanoparticles. © 2014 Springer Science+Business Media, New York.