MIDDLETON, WI, United States
MIDDLETON, WI, United States
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Vogt B.,Prairie Technologies, Inc. | Long Y.A.N.,Prairie Technologies, Inc. | Szulczewski M.,Prairie Technologies, Inc. | Eliceiri K.,University of Wisconsin - Madison
Laser Focus World | Year: 2010

A new technology called swept field confocal (SFC) uses optics to split the excitation laser into multiple beams for improved live cell imaging studies. Galvonometric and piezo-controlled mirrors sweep the image of the selected 32 pinhole column or single slit across the sample. The emission photons are de-scanned and match the optical recording with the temporal biological fluorescence response has great promise for cell and developmental biology studies in which live dynamic events must be captured quickly in high resolution. Key advantages of the SFC design enabled by the imaging optics include high-speed imaging capability, high quantum efficiency (QE) detection and low phototoxicity, variable pinhole sizes or slits, and reduced crosstalk between confocal apertures. The SFC scanning head can be attached to a variety of commercial microscope stands including both inverted and upright microscope models. The SFC uses an innovative design to achieve real-time video rate speeds during acquisition.

Ritt P.,University of Wisconsin - Madison | Lu-Steffes O.,University of Wisconsin - Madison | Sakidja R.,University of Wisconsin - Madison | Sakidja R.,University of Missouri - Kansas City | And 4 more authors.
Journal of Thermal Spray Technology | Year: 2013

Thermal spray methods offer a versatile and flexible approach to the manufacture of coatings as a final product. A novel application of thermal spray coating is demonstrated by incorporating a plasma-sprayed Mo layer coating as a precursor step within an integrated costing design. The effectiveness of the two-step design is illustrated for aluminoborosilica coatings on SiC/C composites and W substrates based on the plasma-sprayed Mo precursor and subsequent codeposition of Si and B by a pack cementation method. Even with incomplete precursor coverage, an aluminoborosilica coating is developed because of the high initial fluidity of the as-pack coating. An effective oxidation resistance is observed following exposure at elevated temperatures (1373-1673 K) in ambient air and during torch testing at 1773 K, providing clear evidence that the plasma spraying of Mo is a viable precursor step in the formation of the oxidation-resistant Mo-Si-B-based coating. © 2013 ASM International.

Prosa T.J.,Imago Scientific Instruments | Keeney S.K.,Prairie Technologies, Inc. | Kelly T.F.,Imago Scientific Instruments
Journal of Microscopy | Year: 2010

Pulsed-laser atom-probe tomography is used to compare the field-evaporation mass spectrum and spatial distribution of molecular fragments from various poly(3-alkylthiophene) films deposited on sharpened aluminium specimen carriers using two different deposition methods. Films deposited via a modified solution-cast methodology yield small fragments with a uniform structural morphology whereas films deposited via an electrospray ionization methodology yield a wide range of fragments with a very non-uniform structural morphology. The main field-evaporated chemical species identified for both deposition types were, in order of typical relative abundance, C2H5+, CH3+, C2H4+, followed by C3H7,8+/SC + and SCH+. Thick electrospray depositions allowed investigation of the influence of laser-pulse energy on the analysis. Evidence is presented supporting the presence of a critical laser-pulse energy whereby changes in film morphology are signalled by the appearance of a new mass fragment at 190 Da. © 2009 The Royal Microscopical Society.

Agency: Department of Health and Human Services | Branch: | Program: SBIR | Phase: Phase II | Award Amount: 1.54M | Year: 2010

DESCRIPTION (provided by applicant): A primary goal in neuroscience is to understand at a mechanistic level the functioning of neuronal networks with specific behaviors. This involves in general, the anatomical connectivity among the various types of neurons within the local network, the physiological properties of these cells, and the spatiotemporal firing signals of the neurons within the circuit. The challenge is not only to discover the connectivity among neurons but also to effectively record the signal activity within the neuronal network during a behavior in such a way as to provide functional information. Since 2001 Prairie Technologies, Inc. has been designing, building, refining and selling a new type of confocal scanning system, the Swept Field Confocal microscope (SFC). SBIR Phase I and II awards have provided the funding necessary for Prairie to develop an entry level product for the biological marketplace. Due to successful completion of all of our Phase I and Phase II goals, we have been able to sell over 60 units totaling to sales of 6,000,000.00 for the company. Recently we have been able to develop a worldwide sales channel for the SFC with Nikon Instruments, Inc. Because of high speed and low toxicity, the SFC is now gaining in sales popularity in the neuro and cell biology fields. In order to provide more functional information, we propose to further improve the speed of the SFC and simplify its ease of use for the scientists and to increase the amount of applications that can be performed with the instrument such as Fluorescence Resonance Energy Transfer (FRET). In particular, we would like to add quantitative techniques such as Fluorescence Lifetime Imaging (FLIM) for the study of dynamic processes and FRET analysis. By completing the specific aims outlined in this proposal, the SFC can not only perform as a confocal microscope, but have capability to provide dynamic information about the local neuron network with high resolution. In addition, with completion of this project Prairie can achieve increased product sales of the SFC into the 200 million annual world-wide laser scanning microscopy market. PUBLIC HEALTH RELEVANCE: A primary goal in neuroscience is to understand at a mechanistic level the functioning of neuronal networks with specific behaviors, which can be used to characterize neurological disorders. Such studies require the ability to identify the signaling networks and noninvasively study the dynamics within living cells. The improved Swept Field Confocal microscope system will provide such capabilities to record the spatiotemporal information in real time of normal and diseased processes and to study the cellular dynamics with additional information about the microenvironment and signaling pathways detected with Fluorescent Lifetime Imaging.

Prairie Technologies, Inc. | Date: 2014-03-24

HVAC components, namely, thermostats, sensors, and automatic zone valves. Development and implementation of software, hardware and technology solutions for the purpose of operation of HVAC unitary equipment.

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