Kórnik, Poland
Kórnik, Poland

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Michalak M.,Pracownia Biologii Nasion | Plitta B.P.,Pracownia Biologii Nasion | Kotlarski S.,Pracownia Biologii Nasion | Chmielarz P.,Pracownia Biologii Nasion
Sylwan | Year: 2014

Cryopreservation (storage in liquid nitrogen, -196°C) is a technique that ensures safe, long-term conservation of plant species with recalcitrant seeds, vegetatively propagated species and biotechnology products such as somatic embryos, cell lines and genetically transformed material. The aim of cryostorage is to keep plant tissue in a safe way, which does not cause changes during storage at neither phenotypic, cytological and histological, biochemical, genetic nor epigenetic level. Therefore, before cryostorage will be applied for long-term preservation it should be thoroughly assess if it does not cause any injuries in plant material. © 2014, Polish Forest Society. All rights reserved.


Plitta B.P.,Pracownia Biologii Nasion | Michalak M.,Pracownia Biologii Nasion | Kotlarski S.,Pracownia Biologii Nasion | Chmielarz P.,Pracownia Biologii Nasion
Sylwan | Year: 2013

Cryopreservation is a method of storage of biological material at the temperature of liquid nitrogen (-196°C, LN). The main advantage of this method is the possibility to store viable cells for a long time. Desiccation and freezing sensitivity of seeds, their fragments or other plant organs, which are useful as genetic resources, should be investigated before cryopreservation.

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