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Biradar S.S.,Veterinary Dispensary | Saravanan B.C.,Indian Veterinary Research Institute | Tewari A.K.,Indian Veterinary Research Institute | Sreekumar C.,Post Graduate Research Institute in Animal science | And 2 more authors.
Acta Parasitologica | Year: 2014

PCR-RFLP and nucleotide sequencing based genotyping of Toxoplasma gondii Indian isolates (Izatnagar and Chennai isolates and Chennai clone) vis-a vis RH-IVRI strain was conducted by targeting GRA6 as genetic marker. The 791 bp GRA6 product was PCR amplified from the genomic DNA of different T. gondii Indian isolates, including the RH-IVRI strain. Tru1I restriction endonuclease based PCR-RFLP of GRA6 sequence produced polymorphic digestion pattern that discriminated the virulent RH-IVRI strain (as type I) from the moderately virulent local isolates as type III. The PCR amplicon of T. gondii GRA6 from RH-IVRI strain as well as from the local isolates were cloned in cloning vector and custom sequenced. The nucleotide and deduced amino acid sequences of T. gondii isolates were aligned with that of the type I, II and III strains (RH, BEVERLEY, ME49, C56, TONT and NED) available in public domain and analyzed in silico using MEGA version 4.0 software. Nucleotide sequencing and phylogenetic analysis of GRA6 marker from the Indian isolates revealed a close genetic relationship with type III strains of T. gondii. Further, detection of a single nucleotide polymorphism (SNP) at positions 162 and 171 of the GRA6 marker, established the lineage of Indian isolates as type III. This is the first report on characterization of T. gondii lineage as type III in selective chicken population of India based on PCR-RFLP and sequence analysis of GRA6 gene. © 2014 Versita Warsaw.


Devendran P.,Post Graduate Research Institute in Animal science | Gopinathan A.,Post Graduate Research Institute in Animal science | Gopinathan A.,Madras Veterinary College | Murugan M.,Post Graduate Research Institute in Animal science | And 2 more authors.
Indian Journal of Animal Research | Year: 2015

Data on body weight of Large White Yorkshire (50%) crosses (n=590) were recorded at weekly intervals from birth to weaning (8 weeks) for five filial generations and were and analysed by least-squares method. The overall mean birth weight was 1.03 ± 0.01 kg. The birth weight gained significantly (P<0.05) over generations and at F5, it was 1.10 ± 0.04 kg. The pooled mean body weights at 2, 4 and 6 weeks and at weaning (8 weeks) were 2.62 ± 0.04, 4.15 ± 0.07, 5.94 ± 0.10 and 8.01 ± 0.10 kg, respectively. The periodical pre-weaning body weights were increased significantly over the generations and at F4, the respective values were 3.06 ± 0.06, 5.00 ± 0.10, 7.26 ± 0.14 and 9.61 ± 0.19 kg. The effect of sex was not found as a significant source of variation on the body weights from birth to weaning. The heritability and genetic correlations found out by sib-analyses for the pre-weaning body weights revealed that the estimates were positive and moderate to high, showing the presence of sufficient genetic variation and favourable associations for these body weights and suggestive of better scope for improvement through selective breeding. © 2015, Agricultural Research Communication Centre. All rights reserved.


Sreekumar C.,Tamil Nadu Veterinary and Animal Sciences University | Sreekumar C.,Post Graduate Research Institute in Animal science | Selvaraj J.,Veterinary College and Research Institute | Gomathinayagam S.,Tamil Nadu Veterinary and Animal Sciences University | And 4 more authors.
Journal of Parasitic Diseases | Year: 2014

Blastocystis, a zoonotic protozoan found in the intestinal tracts of a wide range of animals, has not been reported from non-human hosts from India so far. Organisms indistinguishable from Blastocystis sp. were identified in the Giemsa stained intestinal scrapings collected from carcasses of piglet and poultry that were brought for necropsy to the Central University Laboratory, Chennai. The ‘central vacuole forms’ of the parasite, with number of nuclei ranging from 1 to 12 were identified. The intensity of infection was low, with less than one organism per oil immersion field, indicating that their presence was unconnected to the cause of death. Caecal scraping was found to be more ideal than duodenal scraping for the diagnosis of Blastocystis, and can be a potential specimen for definitive diagnosis. Identical organisms were also detected in the dung samples of a buffalo calf which showed clinical signs of diarrhoea The presence of Blastocystis in food animals acquires public health significance, as many subtypes of the parasite from poultry and pigs are transmissible to humans. © 2013, Indian Society for Parasitology.


Anilkumar R.,Tamil Nadu Veterinary and Animal Sciences University | Anilkumar R.,Veterinary College and Research Institute | Iyue M.,Tamil Nadu Veterinary and Animal Sciences University | Venkataramanan R.,Tamil Nadu Veterinary and Animal Sciences University | Venkataramanan R.,Post Graduate Research Institute in Animal science
Indian Journal of Animal Sciences | Year: 2015

The present work was undertaken to study the growth and hatch performance of geese maintained at Sheep Breeding Research Station, Sandynallah. The average clutch size was 7.18±0.14. The mean hatchability percentage was 62.35±1.92. The mean body weight at hatching was 90.49±0.46 g. The year, seasons and age of dam had significant effect on hatch weight. The highest body weight gain was recorded from fourth week to second month of age. The ADG was significantly affected by year, season, age of the dam and sex in most of the growth phases. Survivability was higher in gosling born during summer and was very poor in north east monsoon period. Hatch weight had significant effect on survivability.


Gopinathan A.,Post Graduate Research Institute in Animal science | Suresh Kumar K.,Post Graduate Research Institute in Animal science | Karthickeyan S.M.K.,Post Graduate Research Institute in Animal science | Ramesh J.,Post Graduate Research Institute in Animal science | Sivaselvam S.N.,Post Graduate Research Institute in Animal science
Indian Journal of Animal Research | Year: 2013

The effect of floor space allocation on growth rate and feed intake of crossbred barrows from 5th to 9th month of age was investigated. Three treatment groups with a floor space of 1.0 m2 (T1), 1.2 m2 (T2) and 1.8 m2 (T3) per pig were maintained and each group contains seven pigs and monthly body weight and feed intake were recorded. Significant difference (P< 0.05) between T1 and other two groups (T2 and T3) was observed with respect to body weight. Significant reduction in body weight (kg) at 9 months of age was observed in T1 group as compared to T2 and T3 groups. Statistically no significant difference was observed among the treatment groups (T2 and T3). But, body weight gain and average daily gain recorded were highest in T2 (1.2 m2/pig) group. Hence, it was concluded that 1.2 m2 floor space/pig proved to be an optimum for finisher pigs to produce better growth rate.


Gunasekaran S.,Tamil Nadu Veterinary and Animal Sciences University | Viswanathan K.,Tamil Nadu Veterinary and Animal Sciences University | Bandeswaran C.,Tamil Nadu Veterinary and Animal Sciences University | Balasubramanyam D.,Center for Animal Production Studies | Venkataramanan R.,Post Graduate Research Institute in Animal science
Range Management and Agroforestry | Year: 2013

A study was undertaken to find out the effect of supplementation of Stylosanthes scabra in coconut based hortipasture model for goats. S. scabra, a leguminous green fodder cultivated in the understorey of coconut trees was used to feed the goats by cut and carry method. Fourteen Boer crossbred male goats raised under intensive method of rearing were divided into two groups viz., Group I (stall feeding with ad libitum tree fodder + concentrates) and Group II (stall feeding with ad libitum tree fodder + concentrates + S. scabra one kilogram/day/ head). There was no significant difference in weight gain between the treatment groups, however the average daily weight gain (g) of goats in the supplemented group was higher (34.7 vs 50.9).


PubMed | Indian Defence Research And Development Laboratory, Madras Veterinary College and Post Graduate Research Institute in Animal science
Type: Journal Article | Journal: Journal of parasitic diseases : official organ of the Indian Society for Parasitology | Year: 2014

The purpose of the study was to detect DNA of Toxoplasma gondii in lymph node aspirates of dogs using amplification of SAG-2 gene fragment. Blood and lymph node aspirates were collected from 20 clinically ill dogs of different age groups. Lymph node aspirates were analysed with polymerase chain reaction (PCR). Two numbers of dogs were found to be positive by PCR. The PCR positive dogs showed modified direct agglutination test (MDAT) titre of 1:512 and 1:4,096. The remaining sera samples showed the titre value of less than 1:64 by MDAT and were also negative by PCR. This study indicates that lymph node aspirates could be an alternative ideal specimen for the detection of T. gondii.


PubMed | Madras Veterinary College and Post Graduate Research Institute in Animal science
Type: Journal Article | Journal: Journal of parasitic diseases : official organ of the Indian Society for Parasitology | Year: 2016

Two combinations of assembly pheromone (AP), with and without hematin were utilized as a lure for the unfed larvae, nymph and adults of


PubMed | Veterinary College and Research Institute, Tamil Nadu Veterinary and Animal Sciences University and Post Graduate Research Institute in Animal science
Type: Journal Article | Journal: Journal of parasitic diseases : official organ of the Indian Society for Parasitology | Year: 2014

Blastocystis, a zoonotic protozoan found in the intestinal tracts of a wide range of animals, has not been reported from non-human hosts from India so far. Organisms indistinguishable from Blastocystis sp. were identified in the Giemsa stained intestinal scrapings collected from carcasses of piglet and poultry that were brought for necropsy to the Central University Laboratory, Chennai. The central vacuole forms of the parasite, with number of nuclei ranging from 1 to 12 were identified. The intensity of infection was low, with less than one organism per oil immersion field, indicating that their presence was unconnected to the cause of death. Caecal scraping was found to be more ideal than duodenal scraping for the diagnosis of Blastocystis, and can be a potential specimen for definitive diagnosis. Identical organisms were also detected in the dung samples of a buffalo calf which showed clinical signs of diarrhoea The presence of Blastocystis in food animals acquires public health significance, as many subtypes of the parasite from poultry and pigs are transmissible to humans.


PubMed | Madras Veterinary College and Post graduate Research Institute in Animal science
Type: Journal Article | Journal: Veterinary world | Year: 2016

The present investigation was undertaken to study the reproduction performance and effect of non-genetic factors on reproduction performance of Jersey crossbred cows.Data on 355 Jersey crossbred cattle maintained at the Post-graduate Research Institute in Animal Sciences, Kattupakkam, Tamil Nadu, distributed over 30 years (1985 to 2014). The effect of various non-genetic factors including the period of birth, season of birth, period of calving, season of calving and parity were analyzed through least-squares analyses using univariate general linear model. The different (co)variance components for calculation of genetic parameters were estimated using restricted maximum likelihood method by fitting an animal model.The overall least-squares means (standard error) of age at first service, age at first calving, weight at first calving, service period, calving interval, dry period, and number of services per conception were 848.069.72 days, 120412.20 days, 289.811.71 kg, 210.016.41 days, 489.126.45 days, 137.965.58 days, 2.500.07, respectively. Period of calving had either significant (p<0.05) or highly significant (p<0.01) effect on all reproduction traits studied except service period, calving interval, and dry period. Number of services per conception was affected by season of calving. Parity had significant influence (p<0.05) or highly significant (p<0.01) influence on all the traits studied except service period and dry period. Heritability estimates of age at first service, age at first calving, weight at first calving, service period, calving interval, dry period, and number of services per conception were 0.299, 0.220, 0.017, 0.142, 0.222, 0.177, and 0.042, respectively. The estimates of repeatability for service period, calving interval, dry period, and number of services per conception were 0.219, 0.234, 0.420, and 0.001, respectively.The reproduction performances of Jersey Red Sindhi crossbreds were lower when compared to the earlier reports on Jersey crossbreds. Heritability and repeatability values were also low to moderate, indicating limited scope for improvement through selection.

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