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Kharkar K.,Post Graduate Institute of Veterinary and Animal science | Kharkar K.,Nagpur Veterinary College | Kuralkar S.V.,Post Graduate Institute of Veterinary and Animal science | Hadge M.R.,Post Graduate Institute of Veterinary and Animal science
Indian Journal of Animal Research | Year: 2015

The data on 481 lactation records including 204 records of Red Kandhari cows and 277 records of Jersey x Red Kandhari crossbred cows were collected from Cattle Unit, College of Veterinary and Animal Sciences, (MAFSU), Parbhani over a period of 27 years (1977-2004). The lactation milk yield for first, second, third, fourth and fifth and above lactations for Red Kandhari cows were 375.16±34.15 kg, 458.17±37.62 kg, 450.91±26.74 kg, 532.69±67.74 kg and 674.29±82.09 kg, respectively and for Jersey x Red Kandhari crossbred were 1100.28±73.18 Kg, 1318.50±135.20 Kg and 1353.92±91.61 Kg, 1549.77±188.47 kg and 1438.37±48.89 kg, respectively. The first lactation length and first dry period in Red Kandhari cows were 273.30±10.33 days and 239.05±21.88 days, while in Jersey x Red Kandhari crossbred cows were 302.68±9.19 days and 86.72±8.90 days, respectively. The least square analysis of variance for first, second and third and more than three lactations for lactation milk yield, lactation length and dry period showed that the genetic group had significant source of variation for all traits in all lactations except first and second lactation length. Sire within genetic group had significant source of variation for first lactation milk yield and all traits of third and more than three lactations. Season and period of calving had non-significant influence on all the traits in all lactations except first dry period. It was also observed that the improved performance in all the traits under study in Red Kandhari cow and Jersey x Red Kandhari crossbred cow was observed in fourth and fifth and above lactations. © 2015, Agricultural Research Communication Centre. All rights reserved.


Kuralkar S.V.,Post Graduate Institute of Veterinary and Animal science | Bankar P.S.,Post Graduate Institute of Veterinary and Animal science | Kuralkar P.,Post Graduate Institute of Veterinary and Animal science
Indian Journal of Animal Sciences | Year: 2014

An attempt was made to envisage physical characteristics, production and reproduction performance of Deoni, a medium size dual purpose indigenous breed of cattle in Maharashtra in its home tract. Chest girth, body length, height at wither, long horn, ear length and face length were measured. The mean values for age at first mating, age at first calving, service period and calving interval were 35.45±0.30 months, 44.50±0.30 months, 116.04±3.21 days and 395.87±3.38 days, respectively.


Mahajan N.,Maharashtra Institute of Pharmacy | Sakarkar D.,Maharashtra Institute of Pharmacy | Manmode A.,Astron Research Ltd | Pathak V.,Post Graduate Institute of Veterinary and Animal science | And 2 more authors.
Advanced Science Letters | Year: 2011

Nanoparticles (NPs) are proposed for targeted drug delivery to the inflammation site in severe cases of inflammatory bowel disease (IBD) where state-of-the-art delivery devices fail. Meselamine (5-Aminosalicylic acid), entrapped into nanoparticles was administered either orally or rectally to male Wistar rats suffering from a preexisting experimental colitis induced by acetic acid. Meselamine, a front line drug in the treatment of ulcerative colitis, was incorporated within poly(lactic-co-glycolic acid) i.e., PLGA nanoparticles, which were administered once a day orally and rectally for five consecutive days. Clinical activity score, colon/body weight ratio, ulcer index and myeloperoxidase activity were determined to assess the inflammation. All nanoparticulate formulations proved to be efficient as compared to the free drug in suspension in mitigating the experimental colitis. The clinical activity score, myeloperoxidase activity and ulcer index decreased significantly (p<0.05*), after the oral administration of meselamine nanoparticles suspension. The free drug, meselamine, suspension does not found effective in terms of clinical activity, myeloperoxidase activity and ulcer index, as is evident from the nonsignificant decrease in clinical parameters. The meselamine nanoparticles proved their potential to retain the drug from systemic absorption as evident by a significantly reduced clinical activity, myeloperoxidase activity and ulcer index. The nanoparticles potential in reducing the inflammation was further proved by the histopathology of the resected colon from every group of animals. The use of drug loaded nanoparticles offers several advantages compared to standard therapeutic strategies such as a higher selectivity in adhesion to and enhanced drug penetration into the inflamed tissue. © 2011 American Scientific Publishers.


Deshmukh S.G.,Post Graduate Institute of Veterinary and Animal science | Pawshe C.H.,Post Graduate Institute of Veterinary and Animal science | Sahatpure S.K.,Post Graduate Institute of Veterinary and Animal science | Patil R.K.,Post Graduate Institute of Veterinary and Animal science
Indian Journal of Animal Sciences | Year: 2010

Cyclic cows (7) were divided into 2 different treatment groups for superovulation. Group 1 (n=4) cows were treated with follitropin-V (400 mg) subcutaneously diluted in 3.2% gelatin carrier vehicle in descending doses and group 2 (n=3) cows were treated with follitropin-V (400 mg) intramuscularly twice daily in descending doses. AU the animals (1.00%) exhibited, superovulatory estrus. The average time required for onset of estrus was 39.5±6.65 h and. 27.33± 10.53 h and the duration of superovulatory estrus was 27.5±9.16 and 26.5±8.81 h in group 1 and 2, respectively. The average number of corpora lutea and unovulatory follicles were H±7.58 and 6.33±2.18 and 2.0±0.70 and 2.66±0.66, in group 1 and 2, respectively. The average total number of embryos and transferable embryos in group 1 was 1.75±1.75 and zero, respectively, while in group 2 it was 5±4.51 and 4±3.15, respectively.


Kuralkar S.V.,Post Graduate Institute of Veterinary and Animal science | Kuralkar P.,Post Graduate Institute of Veterinary and Animal science | Dhaware S.A.,Post Graduate Institute of Veterinary and Animal science | Bankar P.S.,Post Graduate Institute of Veterinary and Animal science | Chopade M.M.,Post Graduate Institute of Veterinary and Animal science
Indian Journal of Animal Research | Year: 2015

Deoni is an important medium heavy dual purpose cattle breed found mainly in Latur district and also distributed in small numbers in the neighboring districts viz. Parbhani, Nanded and Osmanabad of Maharashtra. Deoni cattle have three strains on the basis of coat colour variation viz. Wannera (clear white with black colour at the sides of the face), Shevera (white body with irregular black spots) and Balankya (clear white with black spots on the lower side of the body). The information on 1682 Deoni cattle belonging to 579 owners from 48 villages of 5 talukas of Latur district of Maharashtra was collected randomly for studying morphometric characteristics as well as production and reproduction traits of three strains. The proportion of Wannera, Shevera and Balankya strains was 49.00 %, 46.29 % and 4.71%, respectively. In adult cattle, the Wannera strain had more chest girth (163 cm), body length (125 cm) and height at wither (126 cm) indicating heavier build-up than other two strains. Balankya strain of Deoni cattle had longer face length (50 cm), horn length (22 cm) and ear length (27 cm) than other two strains of Deoni. Balankya strain yielded more milk per lactation (973.63±55.18 kg) as compared to Wannera (830.80±40.38 kg) and Shevera (848.44±41.57 kg) strains and also had more lactation length (258.04±16.28 days). Balankya strain, also had higher fat % (4.08±0.60) and SNF % (8.45±0.19) than other two strains of Deoni cattle. The results revealed that Balankya strain of Deoni cattle had higher milk yield, lactation length and fat percent than Wannera and Shevera strains, but had slightly delayed age at first mating and age at first calving. © 2015, Agricultural Research Communication Centre. All rights reserved.


Sahatpure S.K.,Post Graduate Institute of Veterinary and Animal science | Pawshe C.H.,Post Graduate Institute of Veterinary and Animal science | Ninawe A.S.,Post Graduate Institute of Veterinary and Animal science
Revista Veterinaria | Year: 2010

There is paucity of information concerning the aspiration vacuum which is inconsistent. To date, the aspiration vacuum has usually been expressed in millimeter of mercury and has varied between 40 to 400 mmHg. However, the exact aspiration vacuum at the top of the needle depends on the construction of the ovum pick up device, the length and the diameter of the tubing system and the diameter of the needle. To study the effect of vacuum pressure on recovery rate and quality of oocytes three different aspiration vacuum pressures (80, 90 and 100 mmHg) were applied. In the present study, the total 36 sessions were carried out in which total 134 animals were aspirated by using three different vacuum pressure (80, 90 and 100). By using 80-mmHg vacuum pressures, total 24 animals were aspirated in 7 sessions, in which total 59 follicles (2.46 follicles/animal and 8.43 follicles/session) were aspirated. The mean number of large, medium and small follicles were 2.29, 3.71 and 2.43, respectively. The total 32 oocytes (1.33 oocytes/animal and 4.57 oocytes/session) were collected, in which the mean number of A, B, C and D quality oocytes were 1.85, 1.00, 1.57 and 0.14, respectively. The average oocytes recovery by using 80-mmHg vacuum pressures was 54.23%. By using 90-mmHg vacuum pressures, total 90 animals were aspirated in 23 sessions, in which total 262 follicles (2.91 follicles/animal and 11.39 follicles/session) were aspirated. The mean number of large, medium and small follicles were 2.74, 5.09 and 3.57, respectively. The total 182 oocytes (2.02 oocytes/animal and 7.91 oocytes/session) were collected, in which the mean number of A, B, C and D quality oocytes were 2.61, 1.96, 1.65 and 1.67, respectively. The average oocytes recovery by using 90mmHg was 69.46 %. By using 100-mmHg, total 20 animals were aspirated in 6 sessions, in which total 58 follicles (2.90 follicles/animal and 9.67 follicles/session) were aspirated. The mean number of large, medium and small follicles were 2.83, 3.83 and 3.00, respectively. The total 37 oocytes (1.85 oocytes/animal and 6.16 oocytes/session) were collected, in which the mean number of A, B, C and D quality oocytes were 2.00, 1.33, 1.50 and 1.33, respectively. The average oocytes recovery by using 100 mmHg was 63.79%. The results revealed that by using 90mmHg vacuum pressure, 11.39 follicles/session were aspirated. In which, average 7.91 oocytes/session were collected, so the total oocyte recovery rate was 69.46%, was better than 80 and 90 mm Hg vacuum pressure, having 54.23% and 63.79% oocytes recovery, respectively.


Ingawale M.V.,Post Graduate Institute of Veterinary and Animal science | Bakshi S.A.,Post Graduate Institute of Veterinary and Animal science | Birade H.S.,Post Graduate Institute of Veterinary and Animal science | Chinchkar S.R.,Post Graduate Institute of Veterinary and Animal science | Gulavane S.U.,Post Graduate Institute of Veterinary and Animal science
Buffalo Bulletin | Year: 2012

The present research was planned to study follicular development and initiation of postpartum ovarian activity ultrasonographically and per-rectally in Murrah buffaloes having retained placentas. A total of 36 buffaloes with retained placentas were divided into three groups of twelve. In the buffaloes from Groups 1 and 2, placentas were removed manually and Furea boluses were kept intra-uterine (3 boluses per day I/U for 3 consecutive days post-partum) while Inj. GnRH (5 ml) and Inj. PGF2 alpha (5 ml) were administred intramuscularly on day 14 post-partum to Groups 1 and 2, respectively. The buffaloes from Group 3 were kept as control. The follicular development and initiation of ovarian activity were monitored on days 14, 21 and 28 post-partum in the experimental buffaloes. Multiple small follicular activity and good follicular activity were not seen on day 14 post-partum. On day 21 post-partum, 17 (47.22%) ovaries showed small follicular development and five (13.88%) ovaries showed multiple follicular activity when observed ultrasonographically. On day 28 post-partum, 11 (30.55%) ovaries indicated good follicular development observed per-rectally while 13 (36.11%) ovaries indicated good follicular development when observed ultrasonographically. The post-partum ovarian activity was initiated in an average of 3.54 weeks in the GnRH treated buffaloes (Group 1) of 3.54 weeks in the PGF2α treated buffaloes (Group 2) and in 4 weeks in the control group.


Hadge M.R.,Post Graduate Institute of Veterinary and Animal science | Kuralkar S.V.,Post Graduate Institute of Veterinary and Animal science | Ali S.Z.,Post Graduate Institute of Veterinary and Animal science | Kharkar K.P.,Post Graduate Institute of Veterinary and Animal science | Sawaimul A.D.,Post Graduate Institute of Veterinary and Animal science
Indian Journal of Animal Research | Year: 2012

The data on 194 lactations of Sahiwal cows and 81 lactations of Sahiwal x Jersey crossbred cows of Bull Mother Farm, Wadsa Dist. Gadchiroli (M.S.) spread over a period 19 years (1989 to 2007) were analyzed by least square techniques to study the effect of sire, season of calving and period of calving on lactation milk yield, lactation length and dry period. Least square analysis of variance shows that period of calving was highly significant for lactation milk yield in Sahiwal and Sahiwal x Jersey crossbreed cows however season of calving had non significant effect on all traits in Sahiwal and Sahiwal x Jersey crossbred cows. Sire had highly significant effect on lactation milk yield and lactation length in the Sahiwal x Jersey crossbreed cows. The overall least squares means for lactation milk yield, lactation length and dry period of Sahiwal and Sahiwal x Jersey crossbred cows were 1122.74±80.74kg and 1391.38±171.51kg; 279.72±6.55 days and 288.49±9.19 days; 247.14±33.69 days and 249.72±21.36 days, respectively. The moderate to high heritability estimates indicate that they can be improved through selective breeding.


Ingawale M.V.,Post Graduate Institute of Veterinary and Animal science | Bakshi S.A.,Post Graduate Institute of Veterinary and Animal science | Birade H.S.,Post Graduate Institute of Veterinary and Animal science | Chinchkar S.R.,Post Graduate Institute of Veterinary and Animal science | Gulavane S.U.,Post Graduate Institute of Veterinary and Animal science
Buffalo Bulletin | Year: 2014

The present research was carried out to study the effect of GnRH and PGF2α administration on uterine involution and post-partum fertility in buffaloes. A total of 36 normally calved Murrah buffaloes were divided into three groups. The buffaloes of Group1 were treated with GnRH (buserelin acetate) 0.020 mg while buffaloes of Group 2 with PGF2α (cloprostenol sodium) 1.30 mg on day 14 post-calving intramuscularly while the buffaloes of Group 3 were kept as control. The average days required for involution of the uterus were 25.08±1.04, 22.75±0.91 and 30.33±1.31 while the average days required for exhibition of first post- partum oestrus were 37.5±5.16, 26.91±1.36 and 45.08±3.77 days in Groups 1, 2 and 3, respectively. The first service conception rate was highest 41.66% in Group 2 while the rates were 33.33 and 16% in Groups 1 and III, respectively. © 2014, Kasetsart University. All rights reserved.


PubMed | P.A. College and Post Graduate Institute of Veterinary and Animal science
Type: | Journal: Human & experimental toxicology | Year: 2016

Modulation of myometrial spontaneity by cadmium (Cd) and its regulatory pathways was studied in rat uterus in the absence and presence of blockers of different signaling pathways. Isometric tension in myometrial strips, under a resting tension of 1 g, mounted in organ bath containing Ringer-Locke solution (RLS) continuously aerated with carbogen, was measured using data acquisition system-based physiograph and Lab Chart Pro V7.3.7 software. Mean integral tension was measured for 8 min. Cd (1 nM-0.1 mM) not only produced concentration-dependent inhibitory effect on rat myometrium but it (10 M) also significantly (p < 0.05) inhibited calcium chloride and BAY K-8644-induced myometrial contraction. Glybenclamide (10 M), 4-aminopyridine (1 mM), and propranolol (10 M) failed to significantly attenuate Cd-induced inhibitory responses, while L-NAME (0.1 mM), 1H-[1,2,4]Oxadiazolo[4,3-a]quinoxalin-1-one (ODQ; 25 M), and 9-(tetrahydro-2-furanyl)-9H-purin-6-amine (SQ 22536; 1 M) significantly (p < 0.05) produced inhibitory effects on Cd-induced myometrial relaxation. Phenylephrine (1 nM-10 M) and salbutamol (0.01 nM-0.1 M)-induced relaxant effects on rat myometrium were significantly potentiated by 10 M Cd. Thus based on the results of present functional study, it may be inferred that inhibitory effects of Cd on rat myometrium are mediated through blockade of L-type calcium channels and activation of NOS-NO-sGC and/or AC-cAMP pathways.

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