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Dehradun, India

Solanki M.,Manav Rachna International University | Garg F.C.,SBS Post Graduate Institute
Brazilian Journal of Microbiology | Year: 2014

A transconjugant of Azotobacter chroococcum Mac 27 tagged with lac Z(A. chroococcum Mac27 L) was found to possess high levels of β-galactosidase activity constitutively.Further, the lac Z marker was found to be stably integrated into the chromosome of the A. chroococcum Mac 27 and did not have any adverse effect on growth, nitrogen fixation and excretion of ammonia. A quick method to determine the viable cell number in broth culture and carrier based inoculants has been developed on the basis of β-galactosidase assay. It was found that there was a direct relationship between the number of cell as determined by standard plate count and intensity of colour that developed upon degradation of ONPG due to β-galactosidase activity. The method was found to be sensitive enough to determine 1.7 x 106 CFU mL-1 in broth culture as well as carrier based Azotobacter inoculants. Further, it was observed that when A. chroococcum Mac27 L was inoculated on Brassica campestris, it could be detected in the presence of other bacteria capable of growing on Burks agar medium containing X-gal on the basis of lac Z genetic marker. © 2014, Sociedade Brasileira de Microbiologia.

Rajput V.,SBS Post Graduate Institute | Santosh Kumar S.,SBS Post Graduate Institute | Arpita,SBS Post Graduate Institute | Kirti,SBS Post Graduate Institute | Abhishek,SBS Post Graduate Institute
Journal of Applied Pharmaceutical Science | Year: 2012

Freshwater cat fish Clarias batrachus was exposed to Butachlor, Imidacloprid and Sodium fluoride with lethal and sub-lethal concentration for 72 hrs and 21 days durations. Protein changes in liver and muscles were analyzed after exposure period. Imidacloprid and sodium fluoride caused remarkable protein loss lethal concentration but at sub-lethal level their toxicity was moderate. But Butachlor caused remarkable protein loss at lethal as well as sublethal conentartion.

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