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Liu F.,Post Entry Quarantine Center for Tropical Plant | Han Y.,Post Entry Quarantine Center for Tropical Plant | Li W.,Post Entry Quarantine Center for Tropical Plant | Shi X.,Hainan University | And 2 more authors.
Crop Protection | Year: 2013

Oncidium is the most popular cut flower for the orchid industry in Hainan Island and several viruses have been reported to negatively impact their growth and yield. A survey of Cymbidium mosaic virus (CymMV) and Odontoglossum ringspot virus (ORSV) in Oncidium orchids cultivated in Hainan Island was conducted during 2009-2012 with indirect enzyme-linked immunosorbent assay (ELISA). A total of 171,585 samples from adult plant, tissue-culture flask, seedling, and medium seedling were tested for CymMV and ORSV. Of the 24,125 adult plant samples from eighteen commercial orchid farms, 77.58% were infected with CymMV, 16.04% with ORSV, and 10.19% with both CymMV and ORSV. Of the 67,080 samples cultivated in tissue-culture flasks from eight tissue culture laboratories, 41.18% were infected with CymMV, 6.11% with ORSV, and 4.46% with both CymMV and ORSV. Of the 54,450 seedling samples from eight seedling nurseries, 53.20% were infected with CymMV, 9.00% with ORSV, and 5.23% with both CymMV and ORSV. And of the other 25,930 medium seedling samples from twelve medium seedling nurseries, 44.96% were infected with CymMV, 7.90% with ORSV, and 5.15% with both CymMV and ORSV. Plant material must be tested for the presence of viruses before being used for mass production by tissue culture, and sanitation during propagation of orchid plants and harvesting of flowers is essential in order to prevent the virus from spreading. These findings emphasize the need to implement a virus-free certification programme that would greatly enhance the management of these two prevalent viruses in the Hainan orchid industry. © 2013 Elsevier Ltd. Source

Liu F.,Post Entry Quarantine Center for Tropical Plant | Feng L.,Inspection and Quarantine Technology Center | Chen X.,BoLuo County Institute of Agricultural science | Han Y.,Post Entry Quarantine Center for Tropical Plant | And 4 more authors.
Journal of Phytopathology | Year: 2012

Banana bunchy top virus (BBTV), Cucumber mosaic virus (CMV), Banana bract mosaic virus (BBrMV) and Banana streak virus (BSV) are four viruses known to infect banana corp. On the basis of the establishment of the optimal PCR and reverse transcription (RT)-PCR for the detection of a single virus, a quadruplex RT-PCR method that employed virus-specific primers was developed for the simultaneous detection and differentiation of all the four viruses in banana. Several sets of primers for each target virus were evaluated for their sensitivity and specificity by simplex and quadruplex RT-PCR. The assay was then validated using banana samples infected with one or more viruses collected from fields and tissue-culture banana industries in Hainan Island. © 2012 Blackwell Verlag GmbH. Source

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