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Alghero, Italy

Mura S.,University of Milan | Greppi G.,University of Sassari | Roggio A.M.,Porto Conte Ricerche | Malfatti L.,University of Sassari | Innocenzi P.,University of Sassari
Microporous and Mesoporous Materials | Year: 2011

Mesoporous titania films have been prepared via evaporation induced self-assembly and used as a matrix to bind linear pentapeptides. The mesoporous surface has been functionalized with amine groups by immersing the samples in a solution of aminopropyltriethoxysilane in toluene. Different pentapeptides have been synthesized for binding to the mesoporous films: H-Ile-Gln-Asp-Leu-Phe- COOH, H-Val-Gln-Asp-Leu-Phe-COOH and Fmoc-Phg-Gln-Asp-Leu-Phe-COOH. The peptides have been bonded to the amine functionalized surface of the titania mesoporous films with an impregnation process. The H-Val-Gln-Asp-Leu-Phe-COOH peptide has been successfully bonded to the titania matrix, while the other peptides have shown not be suitable for the process because of lower solubility and sterical hindrance. The functionalization with aminopropyltriethoxysilane and peptide binding has been studied by Fourier transform infrared spectroscopy and fluorescence spectroscopy. A fluorescent marker, fluorescein isothiocyanate, has been used to confirm the incorporation of the peptides into the titania matrix. The process is reliable and robust, after several washing cycle of the samples, the peptides are still well bonded to the titania mesoporous films. © 2010 Elsevier Inc.

Sanna V.,Porto Conte Ricerche | Sechi M.,University of Sassari
Nanomedicine: Nanotechnology, Biology, and Medicine | Year: 2012

The application of nanotechnology in medicine is offering many exciting possibilities in healthcare. Engineered nanoparticles have the potential to revolutionize the diagnosis and the therapy of several diseases, particularly by targeted delivery of anticancer drugs and imaging contrast agents. Prostate cancer, the second most common cancer in men, represents one of the major epidemiological problems, especially for patients in the advanced age. There is a substantial interest in developing therapeutic options for treatment of prostate cancer based on use of nanodevices, to overcome the lack of specificity of conventional chemotherapeutic agents as well as for the early detection of precancerous and malignant lesions. Herein, we highlight on the recent development of nanotechnology strategies adopted for the management of prostate cancer. In particular, the combination of targeted and controlled-release polymer nanotechnologies has recently resulted in the clinical development of BIND-014, a promising targeted Docetaxel-loaded nanoprototype, which can be validated for use in the prostate cancer therapy. However, several limitations facing nanoparticle delivery to solid tumours, such as heterogeneity of intratumoural barriers and vasculature, cytotoxicity and/or hypersensitivity reactions to currently available cancer nanomedicines, and the difficult in developing targeted nanoparticles with optimal biophysicochemical properties, should be still addressed for a successful tumour eradication. © 2012 Elsevier Inc.

Sanna V.,University of Sassari | Roggio A.M.,Porto Conte Ricerche | Pala N.,University of Sassari | Marceddu S.,CNR Institute of Sciences of Food Production | And 3 more authors.
International Journal of Biological Macromolecules | Year: 2015

The polyphenols as nutraceutical and therapeutic agents are gaining growing interest for their beneficial effects and potential in human health. In order to protect their scaffolds and functionality, and to improve the bioavailability, the microencapsulation can represent a promising strategy.This study reports on the formulation of the natural resveratrol (RSV) into microcapsules (MCs) prepared by using different concentrations of chitosan (CS) and poly(D, L-lactic-co-glycolic acid) (PLGA) as polymeric matrix. MCs were prepared by W/O/W double emulsion method and characterized in terms of morphology, size, encapsulation efficiency, physicochemical and thermal properties. RSV release behavior from MCs was evaluated under simulated gastrointestinal fluids, and the long term stability was monitored at different storage conditions.MCs resulted to have spherical shape and different morphology, with size ranging from 11 to 20μm, and encapsulation efficiencies of 40-52%, depending on the CS concentration. Moreover, MCs containing CS exhibited a significant lower release of RSV than those containing only PLGA. Furthermore, all tested formulations were able to ensure a good retention and stability of encapsulated RSV until 6 months.In summary, CS/PLGA MCs can be proposed as an attractive delivery system to control the release and long term protection of RSV. © 2014 Elsevier B.V.

Tanca A.,University of Sassari | Deligios M.,University of Sassari | Addis M.F.,Porto Conte Ricerche | Uzzau S.,University of Sassari
Journal of Infection in Developing Countries | Year: 2013

New technologies have shown significant promise in the fight against infectious diseases, with the discovery of novel molecular targets for in vitro diagnostics and the improved design of vaccines. In developing countries, especially in areas of neglected diseases and resources-poor settings, a number of technological innovations are further needed, such as the integration of old and new biomarkers in suitable analysis platforms, the simplification of existing analysis systems, and the improvement of sample preservation and management. However, in these areas, identification of new biomarkers for infectious diseases is still a core issue in the diagnostic quest. Similarly, new technologies will allow scientists to design vaccines with improved immunogenicity, efficacy and safety in the local area, according to the circulating pathogenic strains and the genetic background of the population to be immunized. In this work we review the current omics-based technologies and their potential for accelerating the development of next generation vaccines and the identification of biomarkers suitable for point-of-care (POC) diagnostic applications. © 2013 Tanca etal.

Zoroddu M.A.,University of Sassari | Medici S.,University of Sassari | Peana M.,University of Sassari | Anedda R.,Porto Conte Ricerche
Dalton Transactions | Year: 2010

A multi-histidinic peptide and its minimal models have been investigated for Zn(ii) binding. We have used NMR spectroscopy to probe the binding of zinc to the three repeats (T1R2S3R4S 5H6T7S8E9G 10)3 and to its mono-histidinic minimal models, the 9- and 10-aminoacid fragment. 1H-1H TOCSY, 1H- 13C HSQC, 1H-1H NOESY and 1H- 1H ROESY multidimensional NMR techniques were performed to understand the details of metal binding sites and the conformational behaviour of the peptides at different pH values and at different ligand to metal molar ratios. Zinc coordination involves imidazole Nδ of His6 and carboxyl γ-O of Glu9 residues; interaction with peptide oxygens of the His6-Thr7 or Thr7-Ser8 bonds in a tetrahedral arrangement with the minimal model peptides, cannot be excluded. Zinc coordination involves, at physiologic pH, all the three imidazole Nδ donors of His6, His16 and His26 as well as carboxyl γ-O of Glu residues in a tetra, penta or octahedral arrangement with the three repeats, the 30-aminoacid fragment. Zinc complexation induces important structural changes with the C-terminal portion of the ligand, constraining it to leave its disordered conformation. Our results give rise to a model of the induced structure of the peptides when bound to zinc. At high pH, amide deprotonation does not take place and hydroxo or high molecular weight polymeric species may be formed. © The Royal Society of Chemistry 2010.

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