Porto Conte Ricerche S.r.l

Alghero, Italy

Porto Conte Ricerche S.r.l

Alghero, Italy
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Santercole V.,Porto Conte Ricerche Srl | Delmonte P.,U.S. Food and Drug Administration | Kramer J.K.G.,Agriculture and Agri Food Canada
Lipids | Year: 2012

Commercial fish oils and foods containing fish may contain trans and/or isomerized fatty acids (FA) produced during processing or as part of prepared foods. The current American Oil Chemists' Society (AOCS) official method for marine oils (method Ce 1i-07) is based on separation by use of poly(ethylene glycol) (PEG) columns, for example Supelcowax-10 or equivalent, which do not resolve most unsaturated FA geometric isomers. Highly polar 100-m cyanopropyl siloxane (CPS) columns, for example SP-2560 and CP Sil 88 are recommended for separation of geometric FA isomers. Complementary separations were achieved by use of two different elution temperature programs with the same CPS column. This study is the first direct comparison of the separations achieved by use of 30-m Supelcowax-10 and 100-m SP-2560 columns for fatty acid methyl esters (FAME) prepared from the same fish oil and fish muscle sample. To simplify the identification of the FA in these fish samples, FA were fractionated on the basis of the number and type of double bonds by silver-ion solid-phase extraction (Ag +-SPE) before GC analysis. The results showed that a combination of the three GC separations was necessary to resolve and identify most of the unsaturated FA, FA isomers, and other components of fish products, for example phytanic and phytenic acids. Equivalent chain length (ECL) values of most FAME in fish were calculated from the separations achieved by use of both GC columns; the values obtained were shown to be consistent with previously reported values for the Supelcowax-10 column. ECL values were also calculated for the FA separated on the SP-2560 column. The calculated ECL values were equally valid under isothermal and temperature-programmed elution GC conditions, and were valuable for confirmation of the identity of several unsaturated FAME in the fish samples. When analyzing commercially prepared fish foods, deodorized marine oils, or foods fortified with marine oils it is strongly recommended that quantitative data acquired by use of PEG columns is complemented with data obtained from separations using highly polar CPS columns. © AOCS 2011.

Cacciotto C.,University of Sassari | Cubeddu T.,University of Sassari | Addis M.F.,Porto Conte Ricerche Srl | Anfossi A.G.,University of Sassari | And 7 more authors.
Cellular Microbiology | Year: 2016

Neutrophil granulocytes are paramount to innate responses as major effectors of acute inflammation. Among the various strategies enacted by neutrophils to eliminate microbes NETosis is a novel distinct antimicrobial activity in which an interlacement of chromatin fibres rich in granule-derived antimicrobial peptides and enzymes is extruded (NETs, neutrophils extracellular traps). NETs contribute to the pathogenesis of acute and chronic inflammatory disorders. The interactions of mycoplasmas and innate immune cells, in particular neutrophil granulocytes, are poorly defined. Here, we describe NET formation in vivo in the mammary gland and milk of sheep naturally infected by Mycoplasma agalactiae. Also, we assess the contribution of liposoluble proteins, the most abundant component of the Mycoplasma membrane, in inducing NETosis. We demonstrate that Mycoplasma liposoluble proteins induce NET release at levels comparable to what observed with other stimuli, such as lipopolysaccharides and phorbol 12-myristate 13-acetate. Stimulation of neutrophils with synthetic diacylated lipopeptides based on the M. agalactiae P48, P80, and MAG_1000 proteins, combined in a mix or used individually, suggests that NETosis might not be dependent on a specific lipopeptide sequence. Also, NETosis is partially abolished when TLR2 is blocked with specific antibodies. The results presented in this work provide evidences for the mechanisms underlying NET activation in mycoplasma infections, and on their contribution to pathogenesis of mycoplasmosis. © 2016 John Wiley & Sons Ltd.

Alzari V.,University of Sassari | Nuvoli D.,University of Sassari | Scognamillo S.,University of Sassari | Piccinini M.,Porto Conte Ricerche S.r.l. | And 6 more authors.
Journal of Materials Chemistry | Year: 2011

Frontal polymerization has been successfully used to synthesize poly(N-isopropylacrylamide) nanocomposite hydrogels containing graphene. The latter was directly achieved by ultrasound treatment of a dispersion of graphite in N-methylpyrrolidone. The dispersion, having the concentration of 2.21 g L-1, was characterized by TEM analysis and mixed with suitable amounts of N-isopropylacrylamide for the synthesis of graphene-containing nanocomposite polymer hydrogels. The nanocomposite hydrogels were analyzed by SEM and Raman spectroscopy, and their swelling and rheological properties were investigated. It was found that graphene strongly influences the swelling ratio, dramatically increasing it, even if present in small amounts. Finally, the rheological properties of the hydrogels were correlated with the graphene content: G′ modulus and complex viscosity were found to increase with increasing nanofiller concentration, thus indicating the occurrence of good interactions between the two phases. Nevertheless, at a high concentration (i.e., 0.13 wt.%), graphene showed a lubrication effect, lowering the rheological parameters and approaching the same pseudoplastic behaviour of the unfilled material. © 2011 The Royal Society of Chemistry.

Agency: European Commission | Branch: FP7 | Program: BSG-SME | Phase: SME-1 | Award Amount: 1.26M | Year: 2008

Whey is the major worl-wide disposal and pollution problem for the cheese making companies. The development of the project will reach the following results: The solution for the profitability management of the whey as by-product. The diversification of the whey product market avoiding strong whey prices fluctuations. Production of a new high added value product (lactic acid) at low cost from whey. The general aim of this proposal is to design a process for the lactic acid production from deproteinized whey. New immobilisation techniques will be applied in fermentation process with near Infrared spectroscopy that will be set as analytical tool to control the process in a quantitative way, at real time. Green chemistry strategies will be used for the simultaneous reaction and extraction of lactic acid in supercritical fluids technology in order to get a whole sustainable and environmental friendly process. The potential market to produce lactic acid at a low cost with higher purity based on new production technologies will suppose a great increase in the demand and the evolution of new applications. Lactic acid and its derivatives are widely used in the pharmaceutical, food, cosmetic, . Recently, in the pharmaceutical filed, there has been an increased interest in lactic acid production, since it can be used as a raw material for production of L(\)-polylactic acid, a polymer used for manufacturing high cost biomedical implants and environmental-friendly biodegradable plastic. This project requires the use of advanced facilities and the simultaneous collaboration of experts in biotechnology, supercritical fluids and processes developers. Three RTD performance has been selected to ensure the achievement of these objectives. At the end of the project relevant information required for the industrial application and exploitation of the solution will be obtained (economic prediction of production cost, chemical process designed)

Malfatti L.,University of Sassari | Falcaro P.,CSIRO | Marmiroli B.,Austrian Academy of Sciences | Amenitsch H.,Austrian Academy of Sciences | And 3 more authors.
Nanoscale | Year: 2011

Mesoporous nanocomposite materials have been fabricated through integration of evaporation-induced self-assembly and deep X-ray lithography. Micropatterned films made using a mesoporous ordered silica matrix which contains silver nanoparticles have been obtained. The exposure of the mesoporous films to high energy X-rays, which are generated by a synchrotron source, produces several effects: the removal of the surfactant, the densification of the silica backbone and the formation of silver nanoparticles. This integrated process produces a nanocomposite material which has a 2D-hexagonal organized porosity and silver nanoparticles with a sharp size distribution around 5 nm. The patterned nanostructured films have been tested as a lab-on-chip device for intrinsic surface enhanced Raman scattering detection using a solution containing rhodamine 6G in ethanol and measuring Raman response as a function of laser power. © The Royal Society of Chemistry 2011.

Soggiu A.,University of Milan | Piras C.,University of Sassari | Bonizzi L.,University of Milan | Hussein H.A.,University of Milan | And 2 more authors.
Acta Diabetologica | Year: 2012

Diabetes is a chronic metabolic disease which can lead to serious health problems particularly in and to the development of cardiovascular and renal complications. The aim of this study is to possibly identify distinctive molecular features in urine samples which might correlate to the progression and complications of type 1 diabetes. Diabetic patients with normo- and micro-albuminuria have been analyzed and compared to a group of control subjects. Urine proteins of control and type 1 diabetes subjects were investigated in their proteome profiles, using high-resolution two-dimensional gel electrophoresis separation and protein identifications by MALDI-TOF-MS and LC-MS/ MS analysis. Proteomics analysis highlighted differential expression of several proteins between control and type 1 diabetes subjects. In particular, five proteins were found to be down-regulated and four proteins up-regulated. Lower protein representations in diabetic subjects were associated with Tamm-Horsfall urinary glycoprotein, apolipoprotein A-I, apolipoprotein E, a2-thiol proteinase inhibitor, and human complement regulatory protein CD59, while higher protein representations were found for a-1-microglobulin, zinc-a2 glycoprotein, a-1B glycoprotein, and retinol-binding protein 4. These differences were maintained comparing control subjects with type 1 diabetes normo-albuminuric and micro-albuminuric subjects. Furthermore, these proteins are correlated to glycosylated hemoglobin and microalbuminuria, confirming their role in diabetic pathology. This study gives new insights on potential molecular mechanisms associated with the complications of type 1 diabetic disease providing evidences of urine proteins potentially exploitable as putative prognostic biomarkers. © Springer-Verlag 2011.

Nuvoli D.,University of Sassari | Valentini L.,University of Perugia | Alzari V.,University of Sassari | Scognamillo S.,University of Sassari | And 4 more authors.
Journal of Materials Chemistry | Year: 2011

In the present work, the use of a commercial ionic liquid as a convenient solvent medium for graphite exfoliation in mild and easy conditions without any chemical modification is presented. To confirm the presence of few layer graphene, its dispersion, which exhibits Tyndall effect, was characterized by Raman and UV spectroscopies, and atomic force and field emission electron microscopies. It is noteworthy that, by gravimetric analysis, a graphene concentration as high as 5.33 mg ml-1 was determined, which is the highest value reported so far in any solvent. © 2011 The Royal Society of Chemistry.

Campus M.,Porto Conte Ricerche Srl
Food Engineering Reviews | Year: 2010

High pressure processing (HPP) allows the decontamination of foods with minimal impact on their nutritional and sensory features. The use of HPP to reduce microbial loads has shown great potential in the meat, poultry and seafood industry. HPP has proven to be a promising technology, and industrial HPP applications have grown rapidly, especially in the stabilization of ready-to-eat meats and cured products, satisfying the demands of regulatory agencies such as the United States Department of Agriculture-Food Safety and Inspection Services (USDA-FSIS). HPP has been investigated for a wide range of operations including non-thermal decontamination of acid foods, combined pressure-heating treatments to inactivate pathogenic bacteria, pressure-supported freezing and -thawing, texturization and the removal of meat from shellfish and crustaceans. Research has also been conducted on the impact of the technology on quality features. Processing-dependent changes in muscle foods include changes in colour, texture and water-holding capacity, with endogenous enzymes playing a major role in the phenomena. This review summarizes the current approaches to the use of high hydrostatic pressure processing, focusing mainly on meat, meat products and seafood. Recent findings on the microbiological, chemical and molecular aspects of HPP technology, along with commercial and research applications, are also described. © 2010 Springer Science + Business Media, LLC.

Pretti L.,Porto Conte Ricerche S.r.l | Bazzu G.,University of Sassari | Serra P.A.,University of Sassari | Nieddu G.,University of Sassari
Food Chemistry | Year: 2014

A simple and rapid method was developed for in vivo simultaneous determination of ascorbic-acid and antioxidant capacity in microdialysates from cladodes of Opuntia ficus-indica (L.) Miller. The method is verified in water-stressed plants, as compared with a well-watered test controls. The microdialysis probe construction and insertion procedure was specifically developed to minimise the tissue trauma of the plant and to obtain optimal dialysis performance. Microdialysis was performed using a flow rate of 3 μL/min and the samples were analysed by HPLC coupled to electrochemical detection of ascorbic-acid and DPPH-determined antioxidant capacity. Our data indicate exponential decay of the concentrations of the analysed compounds as a function of microdialysis sampling time. Water-stressed Opuntia show decreased ascorbic acid levels and increased the others antioxidants. © 2013 Elsevier Ltd. All rights reserved.

Pinna A.,University of Sassari | Malfatti L.,University of Sassari | Piccinini M.,Porto Conte Ricerche S.r.l. | Falcaro P.,CSIRO | Innocenzi P.,University of Sassari
Journal of Synchrotron Radiation | Year: 2012

The protection of organic and hybrid organic-inorganic materials from X-ray damage is a fundamental technological issue for broadening the range of applications of these materials. In the present article it is shown that doping hybrid films with fullerenes C60 gives a significant reduction of damage upon exposure to hard X-rays generated by a synchrotron source. At low X-ray dose the fullerene molecules act as radical scavengers, considerably reducing the degradation of organic species triggered by radical formation. At higher doses the gradual hydroxylation of the fullerenes converts C60 into fullerol and a bleaching of the radical sinking properties is observed. © 2012 International Union of Crystallography.

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