Pomology Institute

Náousa, Greece

Pomology Institute

Náousa, Greece
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Drogoudi P.D.,Pomology Institute | Pantelidis G.,Pomology Institute | Bacchetta L.,ENEA | De Giorgio D.,Italian Agricultural Research Council | And 3 more authors.
International Journal of Food Sciences and Nutrition | Year: 2013

Almond protein and potassium (K), phosphorus (P), calcium (Ca) and magnesium (Mg) contents were determined in 72 cultivars and accessions grown in France, Greece and Italy, as part of the networking of European SAFENUT AGRI GEN RES project, which aimed to explore and valorize the almond genetic resources in Europe. Great variation was found in the nutrient content and the amount of nutrient supplied when consuming the recommended daily amount of one serving of almond, among the different genotypes assayed. The variation among the different genotypes was greatest for Ca, followed by the protein content; the latter also exhibited the lowest variation considering the harvest year. Results from a principal component analysis showed that P and Mg were the most discriminant elements for categorizing samples. Cluster analysis showed groups of samples with interesting characteristics for breeding. There was no clear distinction among the different origins of samples. Correlation analyses between weather conditions and the nutrients assayed showed that the mean temperature recorded in the period between March and September was positively correlated with Ca and P only in France, a place where the greatest climatic difference between years was observed. © 2012 Informa UK, Ltd.


Bacchetta L.,ENEA | Rovira M.,IRTA - Institute of Agricultural-Alimentary Research and Technology | Tronci C.,ENEA | Aramini M.,ENEA | And 7 more authors.
Genetic Resources and Crop Evolution | Year: 2015

During recent years, there has been increasing awareness of the importance of adopting a holistic view of biodiversity, including agricultural biodiversity, conservation for sustainable utilization and development. These principles have been underlined in the Convention on Biological Diversity and the European efficiency resources towards 2050. Thus critical issues are now to understand the distribution and extent of genetic diversity available to breeders and stakeholders, the kind and range of characterization, how to face the problem of continuous expanding of germplasm to be conserved. Focusing on the case study of hazelnut which is a crop of great importance for European countries, the paper describes a resourceful strategy for re-organizing and sharing hazelnut genetic resources through an upgrading of knowledge on their value and uses. The paper summarizes the progresses so far and provides a ‘launching pad’ for future researches. The brief review discusses also the recent progresses in recovery, characterization conservation and uses of European hazelnut germplasm achieved by 068 AGRI GEN RES SAFENUT which was one of the 17 Action financed by the European Commission—Directorate General for Agriculture and Rural Development. The current status on the morphological and molecular characterization of the in situ and ex situ of the most important European collections, the rescue and preservation of new accessions recovered on farm were discussed underling critical aspects. A better understanding of hazelnut genetic diversity and its distribution is essential for its conservation and use as well as the harmonization of the morphological and biochemical descriptors. The importance of traditional knowledge is also considered as integrated part of the multidisciplinary approach useful to rationalize genetic resources maintained in the collections. Thus improving the characterization on cultivated and wild forms through the development of a core collection, is the further step to achieve a more effective management and use of European nuts germplasm. © 2014, Springer Science+Business Media Dordrecht.


Boccacci P.,CNR Institute of Plant virology | Boccacci P.,University of Turin | Aramini M.,ENEA | Valentini N.,University of Turin | And 13 more authors.
Tree Genetics and Genomes | Year: 2013

Hazelnut (Corylus avellana L.) is a traditional nut crop in southern Europe. Germplasm exploration conducted on-farm in five countries (Portugal, Spain, Italy, Slovenia, and Greece) identified 77 landraces. The present work describes phenotypic variation in nut and husk traits and investigates genetic relationships using ten simple sequence repeat (SSR) markers among these landraces, 57 well-known references cultivars, and 19 wild accessions. Among the 77 landraces, 42 had unique fingerprints while 35 showed a SSR profile identical to a known cultivar. Among the 42 unique landraces, morphological observations revealed high phenotypic diversity, and some had characteristics appreciated by the market such as nut round and caliber. Analysis of genetic relationships and population structure allowed investigation of the origin and spread of the cultivated germplasm in southern Europe. Our results indicate the existence of three primary centers of diversity in the Mediterranean basin: northwestern Spain (Tarragona) and southern Italy (Campania) in the West and Black Sea (Turkey) in the East. Moreover, the data suggest the existence of secondary gene pools in the Iberian (Asturias) and Italian (Liguria and Latium) Peninsulas, where local varieties were recently domesticated from wild forms and/or from introduced ancient domesticated varieties. © 2013 Springer-Verlag Berlin Heidelberg.


Bacchetta L.,ENEA | Aramini M.,ENEA | Zini A.,ENEA | Di Giammatteo V.,Applicate | And 6 more authors.
Euphytica | Year: 2013

In the frame of SAFENUT AGRI GEN RES Action, which was a European strategy for the recovery, characterization and conservation of genetic resources, the fatty acids and the tocopherol profiles of a set of 75 hazelnut accessions were analyzed. The aim of this study was to assess the genetic differences among the European germplasm, contributing to the definition of nut quality in traditional European areas of cultivation. Significant differences were found between accessions for oil amount and contents of most fatty acids. As expected, monounsaturated fatty acids made up the largest portion (mean 80.85 %) followed by polyunsaturated fatty acids (10.70 %). The saturated ones were the minor components and accounted for only 8.43 % of the total fatty acids. On the basis of Student's test, significant differences between the 2 years of harvest were found for fatty acid content, except for linoleic acid, the ratio of polyunsaturated, α-tocopherol and the stability index. When the oil content was studied in cultivars from the same site of cultivation, the mean values of the genetic pools from central Italy (60.8 %), Slovenia (59.3 %) and Portugal (58.2 %) showed highest values than those of cultivars grown in Greece (56.8 %), Spain (55.9 %) and France (51.5 %). A chemometric approach based on principal component and clustering analyses was developed to identify the most interesting cultivars for breeding programs. © 2013 Springer Science+Business Media Dordrecht.


Kafkaletou M.,Agricultural University of Athens | Christopoulos M.V.,Agricultural University of Athens | Ktistaki M.-E.,Agricultural University of Athens | Sotiropoulos T.,Pomology Institute | Tsantili E.,Agricultural University of Athens
Journal of Applied Botany and Food Quality | Year: 2015

The effects of rain cover on tree yield, fruit cracking and quality of two cherry (Prunus avium L.) cultivars, 'Adriana' and 'Noire de Meched' ('NM'), were investigated. Cherry quality was determined at harvest and after storage at 1 oC in air for up to 21 d. Rates of CO2 production and O2 uptake were determined at weekly intervals during and after storage. A taste panel rated quality attributes of 'NM' after 14 d storage. The results showed that 'Adriana' was completely, but 'NM' moderately resistant to cracking, while covering prevented the symptom. Respiration rates showed immediate and continuous cooling requirements of all fruit. In 'Adriana', covering reduced weight loss during the first 7-d storage, and promoted the peel colour (PC) and total soluble solids subsequently. Under different environ-mental conditions, PC and total phenolics were advanced in un-covered 'NM' at harvest and after storage. In both cultivars, covering did not affect negatively the yield, nor did it significantly the fruit weight, firmness, titratable acidity, pH value, total antioxidant acti-vity, stem browning and resistance to stem removal. 'Adriana' and 'NM' retained good quality for 21 and 14 d storage, respectively. © 2015 The Author(s).


Ganopoulos I.,Center for Research and Technology Hellas | Ganopoulos I.,Aristotle University of Thessaloniki | Merkouropoulos G.,Center for Research and Technology Hellas | Pantazis S.,Pomology Institute | And 3 more authors.
Genetics and Molecular Research | Year: 2011

Quince is a deciduous tree known to the countries around the Mediterranean since antiquity. Nowadays, quince is used as an ornamental plant, and as a rootstock for pear trees, with its fruit being appreciated mainly for production of jam and sweets rather than for raw consumption. Quince leaves contain compounds with antioxidant, antimicrobial and anticancerous properties that have been the focus of recent research on pharmaceutical and medical uses as well as for food preservatives. An orchard has been established in Greece, composed of quince varieties (Cydonia oblonga, N = 49) collected from different sites of the country (mainly from home gardens), constituting a unique quince gene bank collection for southeast Europe. We made a phenotypic analysis using 26 morphological plus seven agronomical descriptors coupled with molecular techniques in order to examine the genetic diversity within the collection. Principal component analysis using the 33 descriptors identified 10 components explaining the existence of more than 70% of the total variation. Subsequent cluster analysis classified most of the previously identified productive varieties of the quince orchard in the same clade of a dendrogram. Molecular analysis generated by 13 inter-simple sequence repeat primers amplified 139 bands, including 109 polymorphic bands, indicating a level of polymorphism of 79%; mean gene diversity was calculated to be 0.309. Using stepwise multiple regression analysis, a number of markers significantly associated with fire blight susceptibility, yield, mean fruit weight, citric acid content, soluble solid content, and fruit drop were identified. Hence, data extracted by multiple regression analysis could be useful in marker-assisted breeding programs, especially when no previous genetic information is available. © FUNPEC-RP.


Tsoktouridis G.,Alexander Technological Educational Institute of Thessaloniki | Tsoktouridis G.,Laboratory for the Conservation and Evaluation of Native and Floricultural Species | Koutinas N.,Alexander Technological Educational Institute of Thessaloniki | Osmantzikidis I.,Alexander Technological Educational Institute of Thessaloniki | And 6 more authors.
Journal of Environmental Protection and Ecology | Year: 2014

Advance in genomics and genetic research in eucalypts is increasing and becomes valuable in resolving problems related to the identification of Eucalyptus taxa, their genetic diversity, phylogeny and phylogeography. Numerous molecular studies have been proved extremely useful in DNA barcoding many Eucalyptus species and hybrids, aiming to determine the accurate genetic classification and origin of eucalypts. Fifteen species of Eucalyptus have been investigated to find genetic differences, using the clpP molecular marker of chloroplast DNA. A total of 390 bp of the clpP intergenic region were amplified by polymerase chain reaction (PCR) for 15 Eucalyptus species and nucleotide differences were determined. A conservative plastid region of the first 83 nucleotides and three variable regions were identified for the 46 Eucalyptus species included in our study. A phylogenetic tree has been constructed based on DNA sequences generated in this study and another 31 available at GenBank.


Ioannis G.,Institute of Applied Biosciences | Ioannis G.,Aristotle University of Thessaloniki | Aliki X.,Institute of Applied Biosciences | Aliki X.,Aristotle University of Thessaloniki | And 6 more authors.
Plant Genetic Resources: Characterisation and Utilisation | Year: 2014

Sweet cherries can be grafted onto a wide range of rootstocks belonging to the genus Prunus. The identification of sweet cherry rootstocks using morphological traits is almost impossible particularly during the dormant season, yet it is very important for the grower to know exactly the rootstock as this has a major influence on cultivar performance and agricultural practices. However, DNA-based molecular analysis carried out on actively growing shoot tips, leaves or dormant buds provides a good opportunity to reliably distinguish the rootstocks. Herein, we have integrated high-resolution melting (HRM) analysis, coupled with five simple sequence repeat (SSR) markers, in order to facilitate the identification of the most popular sweet cherry rootstocks in Greece. The five SSR loci used were highly informative and generated a unique melting curve profile of microsatellites for each of the six sweet cherry rootstocks tested. In particular, one marker, BPPCT002, with six HRM profiles was sufficient to discriminate all the sweet cherry rootstocks studied, highlighting its potential use for rootstock identification. Hence, this assay provided a flexible, cost-effective and closed-tube microsatellite genotyping method well suited to sweet cherry rootstock identification. © 2013 NIAB.


PubMed | Pomology Institute
Type: Journal Article | Journal: International journal of food sciences and nutrition | Year: 2013

Almond protein and potassium (K), phosphorus (P), calcium (Ca) and magnesium (Mg) contents were determined in 72 cultivars and accessions grown in France, Greece and Italy, as part of the networking of European SAFENUT AGRI GEN RES project, which aimed to explore and valorize the almond genetic resources in Europe. Great variation was found in the nutrient content and the amount of nutrient supplied when consuming the recommended daily amount of one serving of almond, among the different genotypes assayed. The variation among the different genotypes was greatest for Ca, followed by the protein content; the latter also exhibited the lowest variation considering the harvest year. Results from a principal component analysis showed that P and Mg were the most discriminant elements for categorizing samples. Cluster analysis showed groups of samples with interesting characteristics for breeding. There was no clear distinction among the different origins of samples. Correlation analyses between weather conditions and the nutrients assayed showed that the mean temperature recorded in the period between March and September was positively correlated with Ca and P only in France, a place where the greatest climatic difference between years was observed.

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