Pomology Holovousy Ltd.

Hořice, Czech Republic

Pomology Holovousy Ltd.

Hořice, Czech Republic

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Paprstein F.,Pomology Holovousy Ltd. | Sedlak J.,Pomology Holovousy Ltd. | Holubec V.,Czech Republic Crop Research Institute
Acta Horticulturae | Year: 2013

Continuing destruction of old plantations and alleys resulted in a serious threat of disappearance of fruit landraces representing the most valuable part of genetic diversity in the Czech Republic. Because of the possible extinction of old sweet cherry cultivars, a program for collecting and long term conservation of fruit landraces was started. Collecting expeditions were directed to areas not influenced by intensive agricultural production and recreational expansion (protected landscape areas, national parks and deserted army areas). Important accessions were localized by Global Positioning System (GPS) and registered in situ. The sweet cherry landraces found showed large variation in tree size, productivity, ripening time, fruit size, fruit quality and disease resistance. Descriptions of 27 important sweet cherry landraces are given in this paper. The information acquired on Czech sweet cherry landraces will broaden the utilization of cherry genetic resources for organic farming or landscaping. The cultivars described were grown historically in the territory of the Czech Republic and constitute a cultural heritage of the region and nation. They represent objects of cultural identity and are an integral part of the cultural landscape.


Bohacenko I.,Food Republic | Pinkrova J.,Food Republic | Komarkova J.,Food Republic | Paprstein F.,Pomology Holovousy Ltd.
Horticultural Science | Year: 2010

Fermentable sugars (the total of glucose, fructose, and sucrose), sorbitol content, dry matter content, and titrable acidity were monitored in fruits of 16 new plum cultivars from the harvests in 2004-2007. General linear model of two-factor grouping with testing of significance of simple contrasts with LSD method was used for statistical evaluation of plum cultivar chemical characteristics. Based on these results the tested cultivars were further divided into groups as regards their future processing into prunes, damson cheese or distillates. The traditional cultivar Italian Prune, grown in parallel, was used as the reference standard. Cultivars Gabrovská, Chrudimer, Elena, Hamanova, and President showed the best results and can be recommended for the production of distillates as well as prunes and damson cheese. Katinka, Anna Späth, and Veeblue are preferable for the production of distillates. Valjevka and Čačanska lepotica are suitable for processing into prunes and damson cheese. The substandard values of the parameters of concern were determined in Čačanska najbolja, Hanita, and Voyageur. Cultivars Bluefree, Stanley, and Valor were then valuated as the worst ones.


Sucha J.,Pomology Holovousy Ltd. | Svobodova L.,Pomology Holovousy Ltd.
Horticultural Science | Year: 2010

During 2006-2008 field surveys were carried out in the important cherry growing areas of the Czech Republic to assess the incidence of Prune dwarf virus and Prunus necrotic ring spot virus in commercial orchards and nurseries. A total of 1,438 samples from 1,198 sweet cherry trees and from 240 sour cherry trees were tested by ELISA for the presence of Prune dwarf virus and Prunus necrotic ring spot virus. The overall average infection level was 17.7%. The most infected species were sour cherry trees (22.5%). The most frequently detected virus was Prune dwarf virus (10.9%). Prunus necrotic ring spot virus occurred in 6.3% of samples. Our study provided an indication of a sanitary status of sweet and sour cherry in commercial orchards and nurseries in the Czech Republic.


Sedlak J.,Pomology Holovousy Ltd. | Paprstein F.,Pomology Holovousy Ltd.
Acta Horticulturae | Year: 2016

The purpose of this study was to develop an efficient in vitro multiplication system for blackberry (Rubus fruticosus L.). A successful in vitro culture technique would provide an alternative method, which can potentially multiply selected blackberry genotypes more rapidly than traditional nursery systems. Two genotypes, 'Čačanska bestrna' and blackberry selection No. 7, were sterilised using 0.15% mercuric chloride as sterilization solution. The use of mercuric chloride had a direct beneficial effect to overcome the contamination from all starting plant material. Four types of modified MS medium containing 1, 2 or 4 mg L-1 6-benzylaminopurine (BAP) or combination of 1 mg L-1 BAP, 0.1 mg L-1 indole-3-butyric acid (IBA) and 0.1 mg L-1 gibberellic acid GA3 were tested in multiplication stage. Generally, the highest proliferation rate was obtained for cultivar 'Čačanska bestrna' that produced 6.3 shoots (longer than 10 mm) on a medium containing 4 mg L-1 BAP. All in vitro shoots started spontaneous rooting on multiplication media after depletion of cytokinines after 1 month of cultivation. High survival (more than 95%) was obtained after acclimatization. The plants established after a period of in vitro culture showed no visible morphological differences compared with conventionally propagated plants. In conclusion, R.fruticosus can be efficiently propagated using a modified MS medium.


Paprstein F.,Pomology Holovousy Ltd. | Sedlak J.,Pomology Holovousy Ltd.
Acta Horticulturae | Year: 2012

Twenty six plum cultivars were evaluated in a germplasm plantation of Research and Breeding Institute of Pomology Holovousy Ltd. The collection was planted at a spacing of 6×5 m on myrobalan rootstock. The results of three-year evaluation of eight important fruit characters are presented. Characters evaluated included: time of ripening, weight of fruit, fruit colour, firmness, taste, separability of stone from flesh, overall quality and incidence of PPV symptoms on fruit. Fruits of 'Topgigant Plus', 'Tophit', 'President' and 'Topend Plus' weighed, on average, more than 50 g. In contrast, the lowest average weight of fruits (lower than 20 g) had cultivar 'Hauszwetsche'. 'Hauszwetsche' and 'Tuleu Gras' ranked the highest in taste. In contrast, cultivar 'Ialomita' was at the limits of acceptability for consumer. From the point of view of overall fruit quality cultivars 'Tuleu Gras' and 'Hauszwetsche' received the highest ranking. Cultivars 'Dambovita' and 'Bucurie' had the flesh, which adhered the most closely to the stone. The fruits of cultivar 'Bucurie' and 'Hauszwetsche' were the most seriously affected by PPV. 64% fruits of 'Bucurie' and 62% fruits of 'Hauszwetsche' developed typical PPV symptoms.


The objective of this study was evaluation of plum cultivar productivity in commercial plantings in first years of cultivation. Plum cultivars were evaluated in three locations with different tree density. High yields were recorded by the cultivar 'Hanita' with cumulated yield after six years of cultivation 102.0 t/ha and by the cultivar 'Elena' 98.1 t/ha. Cultivars 'Hanita', 'Elena', 'Katinka' and 'Tophit' were evaluated only six years due to the young planting. Cultivars 'President' with cumulated yield 198.8 t/ha (in location Končina A), 'Stanley' with 156.2 t/ha, 'Opal' with 114.0 t/ha and 'Presenta' with 82.1 t/ha were evaluated as the best cultivars after eight years of cultivation. Characteristics of fruits were recorded in recently grown cultivars. Results confirm higher production of newly cultivated cultivars in modern high dense plantings.


Paprstein F.,Pomology Holovousy Ltd. | Sedlak J.,Pomology Holovousy Ltd. | Kaplan J.,Pomology Holovousy Ltd.
Acta Horticulturae | Year: 2016

A considerable amount of red and white currant cultivars were cultivated until the beginning of the 20th century in the Czech Republic. Cultivars may be classified under several species. Key species include Ribes rubrum (most red currants), R. petraeum (red currants) and R. multiflorum (white and red currants). Historically, currant cultivars were grown on the territory of the Czech Republic as objects of cultural identity and an integral part of cultural landscape. However, displacement of traditional currant landraces in modern agricultural systems and destruction of old plantations contributed to a considerable loss of genetic diversity. This serious threat of currant diversity losses has resulted in the development of the programme for in situ searching for rescue and long term maintenance of genetic resources in the Czech Republic. The genetic diversity of Czech red and white currant genetic resources has been investigated using phenotypical characterization. This phenotypical characterization revealed high diversity of analyzed germplasm. Genotypes varied in shrub size, fruit yield, ripening time, fruit size and fruit quality. Selected important currant genotypes from the point of view of genetic diversity are described in the paper.


Sedlak J.,Pomology Holovousy Ltd. | Paprstein F.,Pomology Holovousy Ltd.
Acta Horticulturae | Year: 2014

The purpose of this study was to develop an efficient micropropagation system for edible cultivars of apple rose (Rosa pomifera). Two genotypes, 'Karpatia' and 'Belecska', were successfully established in vitro using mercuric chloride or sodium hypochlorite as sterilization solutions. In total only 19.2% of the initial explants of both genotypes were found to be contaminated with micro-organisms, all from explants treated with sodium hypochlorite. Six MS-based media containing 1, 2 or 4 mg L-1 BAP (6-benzylaminopurine), 0.5 or 1 mg L-1 TDZ (thidiazuron) or 10 mg L-1 2iP (6-(γ,γ-dimethylallylamino) purine) were tested for initiation of multiplication. Generally, the highest proliferation rate (5.1) was obtained for 'Belecska' on MS medium with the highest concentration of BAP (4 mg L-1). The lowest shoot number (1.1) was noted for cultivar 'Karpatia' on MS medium with 1 mg L-1 TDZ. Abundant callus formation at the base of explants and abnormally narrow undeveloped leaves were observed on the media containing TDZ with 'Karpatia'. The cytokinin 2iP at 10 mg L-1 did not promote satisfactory proliferation in the two apple rose genotypes tested in our experiments. Rooting was achieved in both genotypes tested, but the root induction was relatively low from 17 to 57% of in vitro rooted plants. Generally, the best rooting (57%) was obtained with cultivar 'Belecska'on medium supplemented with 1 mg L-1 NAA. On the contrary, cultivar 'Karpatia' had the lowest rooting percentage 17% on medium with 1 mg L-1 IAA.

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