Pomeranian Medical Academy

Szczecin, Poland

Pomeranian Medical Academy

Szczecin, Poland
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Gundermann K.-J.,Pomeranian Medical Academy | Kuenker A.,Elk Rapids Medical Clinic | Kuntz E.,Scientific Institute of Hepatology | Drozdzik M.,Pomeranian Medical Academy
Pharmacological Reports | Year: 2011

Essential phospholipids (EPL) contain a highly purified extract of polyenylphosphatidylcholine (PPC) molecules from soybean. The main active ingredient is 1,2-dilinoleoylphosphatidylcholine (DLPC), which differentiates it from other phospholipids, lecithins, or extracts from other sources. Although EPL is widely used in liver diseases of various origins, its mode of action and pharmacological and clinical evidence of its efficacy have not yet been concisely reviewed. This paper critically summarizes experimental and clinical results. With regard to in-vitro and animal tests, EPL influenced membrane-dependent cellular functions and showed anti-oxidant, antiinflammatory, anti-fibrotic, apoptosis-modulating, regenerative, membrane-repairing and -protective, cell-signaling and receptor-influencing, as well as lipid-regulating effects in intoxication models with chemicals or drugs. Clinical studies, primarily from European and Asian countries, have shown improvement in subjective symptoms; clinical, biochemical and imaging findings; and histology in liver indications such as fatty liver of different origin, drug hepatotoxicity, and adjuvant in chronic viral hepatitis and hepatic coma. The available studies characterize EPL as evidence-based medicine, although further long-term controlled clinical trials are required to precisely determine its benefit for alleviating symptoms, improving well-being, inducing histological changes and slowing the progression of liver disease. EPL-related relevant side effects were not observed. Copyright © by Institute of Pharmacology Polish Academy of Science.

Zwierko T.,University Of Szczecin | Osinski W.,Academy of Physical Education in Poznan | Lubinski W.,Pomeranian Medical Academy | Czepita D.,Pomeranian Medical Academy | Florkiewicz B.,University Of Szczecin
Journal of Human Kinetics | Year: 2010

Volleyball is a dynamic game which requires a high level of visual skills. The first aim of this study was to investigate the several aspects of reaction times (RT) to visual stimuli in volleyball players (12) compared to non-athletic subjects (12). By using the tests included in the Vienna Test System (Schuhfried, Austria), simple reaction time (SRT), choice reaction time (CRT) and peripheral reaction time (PRT) were examined. The second aim of this study was to assess the neurophysiological basis of early visual sensory processing in both examined groups. We measured two sets of pattern-reversal visual evoked potentials (VEPs) during monocular central field stimulation (Reti Scan, Roland Consult, Germany). The latencies of waves N75, P100 and N135 were determined. We observed significantly shorter (p < 0.05) total reaction time to stimuli appearing in the central and peripheral field of vision in the volleyball players compared to non-athletes. With regard to SRT and CRT the main differences between the groups appeared in pre-motor reaction times. Volleyball players had shorter VEPs P100 wave latencies (p < 0.05) than the non-athlete group. The results indicate faster signal transmission in visual pathways in athletes than in non-athletes. This fact can be attributed to the effect of rapid visual-activity-demanding sports on the central nervous system. © Editorial Committee of Journal of Human Kinetics.

PubMed | Danish Cancer Society, University of Houston, Cedars Sinai Medical Center, Harvard University and 25 more.
Type: Journal Article | Journal: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology | Year: 2016

While numerous susceptibility loci for epithelial ovarian cancer (EOC) have been identified, few associations have been reported with overall survival. In the absence of common prognostic genetic markers, we hypothesize that rare coding variants may be associated with overall EOC survival and assessed their contribution in two exome-based genotyping projects of the Ovarian Cancer Association Consortium (OCAC).The primary patient set (Set 1) included 14 independent EOC studies (4,293 patients) and 227,892 variants, and a secondary patient set (Set 2) included six additional EOC studies (1,744 patients) and 114,620 variants. Because power to detect rare variants individually is reduced, gene-level tests were conducted. Sets were analyzed separately at individual variants and by gene, and then combined with meta-analyses (73,203 variants and 13,163 genes overlapped).No individual variant reached genome-wide statistical significance. A SNP previously implicated to be associated with EOC risk and, to a lesser extent, survival, rs8170, showed the strongest evidence of association with survival and similar effect size estimates across sets (Pmeta = 1.1E-6, HRSet1 = 1.17, HRSet2 = 1.14). Rare variants in ATG2B, an autophagy gene important for apoptosis, were significantly associated with survival after multiple testing correction (Pmeta = 1.1E-6; Pcorrected = 0.01).Common variant rs8170 and rare variants in ATG2B may be associated with EOC overall survival, although further study is needed.This study represents the first exome-wide association study of EOC survival to include rare variant analyses, and suggests that complementary single variant and gene-level analyses in large studies are needed to identify rare variants that warrant follow-up study. Cancer Epidemiol Biomarkers Prev; 25(3); 446-54. 2016 AACR.

Kladna A.,Pomeranian Medical Academy | Michalska T.,Institute of Physics | Berczynski P.,Institute of Physics | Kruk I.,Institute of Physics | Aboul-Enein H.Y.,National Research Center of Egypt
Luminescence | Year: 2012

Tetracyclines are the second most common antibiotic family in medicine usage. These antibiotics exhibit antioxidant potential; however, the exact mechanism remains unclear. The antiradical activity of the seven tetracyclines (TCs; tetracycline, chlortetracycline, oxytetracycline, doxocycline, methacycline, demeclocycline, minocycline) was determined using the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH ̇) and hydroxyl radicals (HO ̇) generated in a Fenton reaction. Electron spin resonance (ESR), ESR spin-trapping, chemiluminescence and spectrophotometry techniques were applied. It was found that the TCs showed high DPPH antiradical activity in the range 26-96% at 2.5 mmol/L concentration. The second-order rate constants for the reaction between HO ̇ and TCs were calculated, in the range (3.6-9.6) × 10 9 L/mol/s. The tetracycline compounds also exhibited a strong decrease in light emission (range 61-85% at concentration of 1 mmol/L). This study also showed that TCs promote the generation of singlet oxygen in the presence of H2O2̇ and Fe(II)/Fe(III) ions. Our findings suggest direct scavenging activity of the examined tetracyclines towards free radicals, and may be relevant to therapeutic strategy. Copyright © 2011 John Wiley & Sons, Ltd.

Kruk I.,West Pomeranian University of Technology | Michalska T.,West Pomeranian University of Technology | Kladna A.,Pomeranian Medical Academy | Aboul-Enein H.Y.,National Research Center of Egypt
Luminescence | Year: 2011

Carazolol [4-(2-hydroxy-3-isopropyl-amino-propoxy)-carbazole], a β 3-adrenoceptor agonist, is clinically used in the treatment of hypertension, cardiac arrhythmias and angina pectoris. Despite the beneficial effect of the drug, its high dose may contribute to cardiotoxicity. This study was conducted to examine whether carazolol can influence hydroxyl radical formation by a Fenton-like reaction [Co(II) + H 2O 2 + HO -] in the presence of ethylenediaminetetraacetic acid. The oxygen free radicals and singlet oxygen ( 1O 2) formation was traced by three different assay methods: chemiluminescence (CL), an electron spin resonance (ESR) spin trapping with 2,2,6,6-tetramethyl-4-piperidine and 5,5-dimethyl-1-pyrroline-1-oxide, and spectrophotometric determination of 1O 2 based on bleaching of p-nitrosodimethylaniline. The effect of hydroxyl radical inhibitors and 1O 2 quenchers on peroxidation of carazolol was also examined. The results indicated that carazolol enhanced the HO radical and 1O 2 formation in a Fenton-like reaction. Copyright © 2010 John Wiley & Sons, Ltd.

Jaholkowski P.,Nencki Institute of Experimental Biology | Mierzejewski P.,Institute of Psychiatry and Neurology | Zatorski P.,Institute of Psychiatry and Neurology | Scinska A.,Medical University of Warsaw | And 5 more authors.
Genes, Brain and Behavior | Year: 2011

Inhibitory effects of passive ethanol exposure on brain neurogenesis have been extensively documented in animal models. In contrast, a role of brain neurogenesis in ethanol self-administration has not been addressed, as yet. The aim of this study was to assess intake of, and preference for, ethanol solutions [2-16% (v/v)] in a mouse model of adult neurogenesis deficiency based on permanent knockout (KO) of cyclin D2 (Ccnd2). Wild type (WT) and Ccnd2 KO mice did not differ in 2% and 4% ethanol intake. The KO group consumed significantly more ethanol in g/kg when offered with 8% or 16% ethanol as compared with the WT controls. The WT and KO mice did not differ in 2% ethanol preference, but the KO group showed a significantly higher preference for 4-16% ethanol. Animal and human studies have suggested that the low level of response to the sedative/hypnotic effects of alcohol is genetically associated with enhanced alcohol consumption. However, in this study, there were no between-genotype differences in ethanol-induced loss of righting reflex. Previous reports have also suggested that high ethanol intake is genetically associated with the avidity for sweets and better acceptance of bitter solutions. However, the KO and WT mice consumed similar amounts of saccharin solutions and the KOs consumed less quinine (i.e. bitter) solutions as compared with the WTs. In conclusion, these results may indicate that Ccnd2 and, possibly, brain neurogenesis are involved in central regulation of ethanol intake in mice. © 2011 The Authors. Genes, Brain and Behavior © 2011 Blackwell Publishing Ltd and International Behavioural and Neural Genetics Society.

Kulig J.,Jagiellonian University | Kolodziejczyk P.,Jagiellonian University | Sierzega M.,Jagiellonian University | Bobrzynski L.,Jagiellonian University | And 8 more authors.
Oncology | Year: 2010

Objective: The aim of this study was to evaluate the efficacy of adjuvant chemotherapy with etoposide, Adriamycin and cisplatin (EAP) after potentially curative resections for gastric cancer. Methods: After surgery, patients were randomly assigned to the EAP or control arm. Chemotherapy included 3 courses, administered every 28 days. Each cycle consisted of doxorubicin (20 mg/m 2) on days 1 and 7, cisplatin (40 mg/m2) on days 2 and 8, and etoposide (120 mg/m2) on days 4, 5, and 6. Results: Of 309 eligible patients, 141 were allocated to chemotherapy and 154 to the supportive care group. Four (2.8%) treatment-related deaths were recorded, including 3 due to septic complications of myelosuppression and 1 due to cardiocirculatory failure. Grade 3 or 4 toxicities were found in 17 (22%) patients. According to the intention-to-treat analysis, the median survival was 41.3 months (95% confidence interval, 24.5-58.2) and 35.9 months (95% confidence interval, 25.5-46.3) in the chemotherapy and control group, respectively (p = 0.398). Subgroup analysis revealed survival benefit from chemotherapy in patients with tumors infiltrating the serosa and in those with 7-15 metastatic lymph nodes. Conclusion: Three cycles of EAP regimen postoperatively offer no survival advantage in gastric cancer patients. Copyright © 2010 S. Karger AG.

Zwierko T.,University Of Szczecin | Czepita D.,Pomeranian Medical Academy | Lubinski W.,Pomeranian Medical Academy
Graefe's Archive for Clinical and Experimental Ophthalmology | Year: 2010

Background: The aim of our study was to assess the effect of physical exercise with increasing intensity on neuroretinal activity in healthy subjects (n=30). Method: We analysed the amplitude and implicit time of b-wave electroretinogram (ERG) responses in two experiments: (1) for a scotopic blue 10 Hz flicker stimulus (rod-mediated responses), and (2) for a photopic white 30 Hz flicker stimulus (cone-mediated responses). Using a cycloergometer, three 10-minute effort-tests with increasing intensity were performed. Each participant was assigned individual workload values (W) below the lactate threshold (40% VO2max), at the lactate threshold (60-65% VO 2max) and above the lactate threshold (80% VO2max). Five ERG recordings were taken: (1) before, (2-4) immediately after the three subsequent efforts and (5) 1 hour after the completion of the last effort. The right eye was selected for monocular stimulation in both experiments. Results: After the first effort (40% VO2max), we observed an increased amplitude (p<0.001) and decreased implicit time of the b-wave (p<0.01) in cone-mediated responses, and no significant effects in rod-mediated responses. Despite the increase in effort intensity, the cone b-wave amplitude remained unchanged, whereas the rod b-wave amplitude significantly decreased after the subsequent efforts (p<0.001). Conclusions: Rod-mediated responses were more susceptible to effort-induced homeostasis disruptions than the cone-mediated responses. The application of ERGs may be used as neurophysiological criteria in defining the cardiovascular status of the physical performance. © 2010 Springer-Verlag.

Barbara N.,Pomeranian Medical Academy | Wojciech L.,Pomeranian Medical Academy | Danuta K.,Pomeranian Medical Academy
Klinika Oczna | Year: 2010

Purpose: Based on available literature, the authors describe the most frequent ocular diseases and symptoms, as well as bioelectrical dysfunction of the visual pathway in patients with Parkinson's disease (PD). Material and methods: Data published in literature available in PubMed library. The most often ocular diseases, symptoms and the bioelectrical dysfunction were diagnosed using routine ophthalmological examination including tear film tests, perimetry, optical coherence tomography (OCT), color and contrast sensitivity tests, and electrophysiological recordings like EOGs, flash, pattern and multifocal ERGs, VEPs. Results: The most frequent ocular diseases in PD are dry eye syndrome and glaucoma. At least 25% of PD patients manifest visual hallucinations. The most prominent bioelectrical dysfunction of the visual pathway was observed in outer layers of the retina (retinal pigmented epithelium, photoreceptors) and it was registered mainly in EOG, PERG and mfERG tests. Conclusions: During examination of PD patents, general ophthalmologists should especially pay attention to diagnosis and treatment of glaucoma and dry eye syndrome. In PD visual deficits may occur without any noticeable changes in the routine ophthalmological examination. Electrophysiological recordings can explain, at least partially, visual dysfunction in course of PD.

Kulig J.,Jagiellonian University | Sierzega M.,Jagiellonian University | Kolodziejczyk P.,Jagiellonian University | Dadan J.,Medical University of Bialystok | And 6 more authors.
European Journal of Surgical Oncology | Year: 2010

Aims: The purpose of this study was to evaluate the effects of overweight on surgical and long-term outcomes in a Western population of patients with gastric cancer (GC). Methods: An electronic database of all patients with resectable GC treated between 1986 and 1998 at seven university surgical centres cooperating in the Polish Gastric Cancer Study Group was reviewed. Overweight was defined as a body mass index (BMI) of 25 kg/m2 or higher. Results: Four hundred and ninety-two of 1992 (25%) patients were overweight. Postoperatively, higher BMI was associated with higher rates of cardiopulmonary complications (16% vs 12%, P = 0.001) and intra-abdominal abscess (6.9% vs 2.9%, P < 0.001). However, other complications and mortality rates were unaffected. The median disease-specific survival of overweight patients was significantly higher (36.7 months, 95% confidence interval (CI) 29.0-44.4) than those with BMI<25 kg/m2 (25.7 months, 95%CI 23.2-28.1; P = 0.003). These differences were due to the lower frequencies of patients with T3 and T4 tumours, metastatic lymph nodes, distant metastases, and non-curative resections. A Cox proportional hazards model identified age, depth of infiltration, lymph node metastases, distant metastases, and residual tumour category as the independent prognostic factors. Conclusions: Overweight is not the independent prognostic factor for long-term survival in a Western-type population of GC. © 2010 Elsevier Ltd. All rights reserved.

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