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Szczecin, Poland

Kulig J.,Jagiellonian University | Kolodziejczyk P.,Jagiellonian University | Sierzega M.,Jagiellonian University | Bobrzynski L.,Jagiellonian University | And 8 more authors.

Objective: The aim of this study was to evaluate the efficacy of adjuvant chemotherapy with etoposide, Adriamycin and cisplatin (EAP) after potentially curative resections for gastric cancer. Methods: After surgery, patients were randomly assigned to the EAP or control arm. Chemotherapy included 3 courses, administered every 28 days. Each cycle consisted of doxorubicin (20 mg/m 2) on days 1 and 7, cisplatin (40 mg/m2) on days 2 and 8, and etoposide (120 mg/m2) on days 4, 5, and 6. Results: Of 309 eligible patients, 141 were allocated to chemotherapy and 154 to the supportive care group. Four (2.8%) treatment-related deaths were recorded, including 3 due to septic complications of myelosuppression and 1 due to cardiocirculatory failure. Grade 3 or 4 toxicities were found in 17 (22%) patients. According to the intention-to-treat analysis, the median survival was 41.3 months (95% confidence interval, 24.5-58.2) and 35.9 months (95% confidence interval, 25.5-46.3) in the chemotherapy and control group, respectively (p = 0.398). Subgroup analysis revealed survival benefit from chemotherapy in patients with tumors infiltrating the serosa and in those with 7-15 metastatic lymph nodes. Conclusion: Three cycles of EAP regimen postoperatively offer no survival advantage in gastric cancer patients. Copyright © 2010 S. Karger AG. Source

Kladna A.,Pomeranian Medical Academy | Michalska T.,Institute of Physics | Berczynski P.,Institute of Physics | Kruk I.,Institute of Physics | Aboul-Enein H.Y.,National Research Center of Egypt

Tetracyclines are the second most common antibiotic family in medicine usage. These antibiotics exhibit antioxidant potential; however, the exact mechanism remains unclear. The antiradical activity of the seven tetracyclines (TCs; tetracycline, chlortetracycline, oxytetracycline, doxocycline, methacycline, demeclocycline, minocycline) was determined using the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH ̇) and hydroxyl radicals (HO ̇) generated in a Fenton reaction. Electron spin resonance (ESR), ESR spin-trapping, chemiluminescence and spectrophotometry techniques were applied. It was found that the TCs showed high DPPH antiradical activity in the range 26-96% at 2.5 mmol/L concentration. The second-order rate constants for the reaction between HO ̇ and TCs were calculated, in the range (3.6-9.6) × 10 9 L/mol/s. The tetracycline compounds also exhibited a strong decrease in light emission (range 61-85% at concentration of 1 mmol/L). This study also showed that TCs promote the generation of singlet oxygen in the presence of H2O2̇ and Fe(II)/Fe(III) ions. Our findings suggest direct scavenging activity of the examined tetracyclines towards free radicals, and may be relevant to therapeutic strategy. Copyright © 2011 John Wiley & Sons, Ltd. Source

Kulig J.,Jagiellonian University | Sierzega M.,Jagiellonian University | Kolodziejczyk P.,Jagiellonian University | Dadan J.,Medical University of Bialystok | And 6 more authors.
European Journal of Surgical Oncology

Aims: The purpose of this study was to evaluate the effects of overweight on surgical and long-term outcomes in a Western population of patients with gastric cancer (GC). Methods: An electronic database of all patients with resectable GC treated between 1986 and 1998 at seven university surgical centres cooperating in the Polish Gastric Cancer Study Group was reviewed. Overweight was defined as a body mass index (BMI) of 25 kg/m2 or higher. Results: Four hundred and ninety-two of 1992 (25%) patients were overweight. Postoperatively, higher BMI was associated with higher rates of cardiopulmonary complications (16% vs 12%, P = 0.001) and intra-abdominal abscess (6.9% vs 2.9%, P < 0.001). However, other complications and mortality rates were unaffected. The median disease-specific survival of overweight patients was significantly higher (36.7 months, 95% confidence interval (CI) 29.0-44.4) than those with BMI<25 kg/m2 (25.7 months, 95%CI 23.2-28.1; P = 0.003). These differences were due to the lower frequencies of patients with T3 and T4 tumours, metastatic lymph nodes, distant metastases, and non-curative resections. A Cox proportional hazards model identified age, depth of infiltration, lymph node metastases, distant metastases, and residual tumour category as the independent prognostic factors. Conclusions: Overweight is not the independent prognostic factor for long-term survival in a Western-type population of GC. © 2010 Elsevier Ltd. All rights reserved. Source

Kruk I.,West Pomeranian University of Technology | Michalska T.,West Pomeranian University of Technology | Kladna A.,Pomeranian Medical Academy | Aboul-Enein H.Y.,National Research Center of Egypt

Carazolol [4-(2-hydroxy-3-isopropyl-amino-propoxy)-carbazole], a β 3-adrenoceptor agonist, is clinically used in the treatment of hypertension, cardiac arrhythmias and angina pectoris. Despite the beneficial effect of the drug, its high dose may contribute to cardiotoxicity. This study was conducted to examine whether carazolol can influence hydroxyl radical formation by a Fenton-like reaction [Co(II) + H 2O 2 + HO -] in the presence of ethylenediaminetetraacetic acid. The oxygen free radicals and singlet oxygen ( 1O 2) formation was traced by three different assay methods: chemiluminescence (CL), an electron spin resonance (ESR) spin trapping with 2,2,6,6-tetramethyl-4-piperidine and 5,5-dimethyl-1-pyrroline-1-oxide, and spectrophotometric determination of 1O 2 based on bleaching of p-nitrosodimethylaniline. The effect of hydroxyl radical inhibitors and 1O 2 quenchers on peroxidation of carazolol was also examined. The results indicated that carazolol enhanced the HO radical and 1O 2 formation in a Fenton-like reaction. Copyright © 2010 John Wiley & Sons, Ltd. Source

Jaholkowski P.,Nencki Institute of Experimental Biology | Mierzejewski P.,Institute of Psychiatry and Neurology | Zatorski P.,Institute of Psychiatry and Neurology | Scinska A.,Medical University of Warsaw | And 5 more authors.
Genes, Brain and Behavior

Inhibitory effects of passive ethanol exposure on brain neurogenesis have been extensively documented in animal models. In contrast, a role of brain neurogenesis in ethanol self-administration has not been addressed, as yet. The aim of this study was to assess intake of, and preference for, ethanol solutions [2-16% (v/v)] in a mouse model of adult neurogenesis deficiency based on permanent knockout (KO) of cyclin D2 (Ccnd2). Wild type (WT) and Ccnd2 KO mice did not differ in 2% and 4% ethanol intake. The KO group consumed significantly more ethanol in g/kg when offered with 8% or 16% ethanol as compared with the WT controls. The WT and KO mice did not differ in 2% ethanol preference, but the KO group showed a significantly higher preference for 4-16% ethanol. Animal and human studies have suggested that the low level of response to the sedative/hypnotic effects of alcohol is genetically associated with enhanced alcohol consumption. However, in this study, there were no between-genotype differences in ethanol-induced loss of righting reflex. Previous reports have also suggested that high ethanol intake is genetically associated with the avidity for sweets and better acceptance of bitter solutions. However, the KO and WT mice consumed similar amounts of saccharin solutions and the KOs consumed less quinine (i.e. bitter) solutions as compared with the WTs. In conclusion, these results may indicate that Ccnd2 and, possibly, brain neurogenesis are involved in central regulation of ethanol intake in mice. © 2011 The Authors. Genes, Brain and Behavior © 2011 Blackwell Publishing Ltd and International Behavioural and Neural Genetics Society. Source

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