Brito G.A.P.,Polytechnic Center |
Nunes E.A.,Polytechnic Center |
Nogata C.,Polytechnic Center |
Yamazaky R.K.,Polytechnic Center |
And 3 more authors.
Cell Biochemistry and Function | Year: 2010
Previous studies have shown that lipids are transferred from lymphocytes (Ly) to different cell types including macrophages, enterocytes, and pancreatic β cells in co-culture. This study investigated whether [ 14C]-labeled fatty acids (FA) can be transferred from Ly to skeletal muscle (SM), and the effects of exercise on such phenomenon. Ly obtained from exercised (EX) and control (C) male Wistar rats were preloaded with the [ 14C]-labeled free FA palmitic (PA), oleic (OA), linoleic (LA), or arachidonic (AA). Radioactively loaded Ly were then co-cultured with SM from the same Ly donor animals. Substantial amounts of FAwere transferred to SM being the profile PA = OA > AA > LA to the C group, and PA > OA > LA > AA to the EX group. These FA were incorporated predominantly as phospholipids (PA=66.75%; OA = 63.09%; LA=43.86%; AA = 47.40%) in the C group and (PA = 63.99% OA = 52.72%; LA = 55.99%; AA = 63.40%) in the EX group. Also in this group, the remaining radioactivity from AA, LA, and OA acids was mainly incorporated in structural and energetic lipids. These results support the hypothesis that Ly are able to export lipids to SM in co-culture. Furthermore, exercise modulates the lipid transference profile, and its incorporation on SM. The overall significance of this phenomenon invivo remains to be elucidated. Copyright © 2010 John Wiley & Sons, Ltd.
Saito S.,Junior College |
Tomonaga N.,Polytechnic Center |
Kuwano R.,Polytechnic University of Mozambique |
Fujii N.,Polytechnic University of Mozambique
Keikinzoku Yosetsu/Journal of Light Metal Welding and Construction | Year: 2012
Recently, the digital inverter type pulse (DP) welding machine has used widely for MIG welding machine. This DP welding machine is superior to the usual welding machines in respect of the workability. However, the handling of DP welding machine and the welding parameters are not known well for the persons concerned in welding at the present time. It is mentioned as an example that the ratio of successful applicants in the welding performance qualification (WPQ) is only a little better compared with before. In this study, the welding parameters of DP welding machine were investigated from the appearance of the bead when the torch angle and welding direction are changed and the droplet transfer phenomenon. In addition, the examples of the optimum welding parameters were examined for the WPQ. The results were as follows. • It was preferred to weld by the forehand angle in MIG welding of aluminium alloy. • Under the standard condition included in DP welding machine, the arc was stable, the sputter decreased, and appearance of the bead was satisfactory. • The validity of examples of the optimum welding parameters was confirmed by the results of the X-ray radiography and the bending test.
PubMed | Indiana University, Polytechnic Center, Laboratory of Cellular and Molecular Cardiology and Federal University of Rio Grande do Sul
Type: | Journal: Scientific reports | Year: 2015
Growing evidence defines macrophages (M) as plastic cells with wide-ranging states of activation and expression of different markers that are time and location dependent. Distinct from the simple M1/M2 dichotomy initially proposed, extensive diversity of macrophage phenotypes have been extensively demonstrated as characteristic features of monocyte-macrophage differentiation, highlighting the difficulty of defining complex profiles by a limited number of genes. Since the description of macrophage activation is currently contentious and confusing, the generation of a simple and reliable framework to categorize major M phenotypes in the context of complex clinical conditions would be extremely relevant to unravel different roles played by these cells in pathophysiological scenarios. In the current study, we integrated transcriptome data using bioinformatics tools to generate two macrophage molecular signatures. We validated our signatures in in vitro experiments and in clinical samples. More importantly, we were able to attribute prognostic and predictive values to components of our signatures. Our study provides a framework to guide the interrogation of macrophage phenotypes in the context of health and disease. The approach described here could be used to propose new biomarkers for diagnosis in diverse clinical settings including dengue infections, asthma and sepsis resolution.