Sharon, MA, United States
Sharon, MA, United States

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Shevtsov M.A.,Russian Academy of Sciences | Galibin O.V.,Saint Petersburg State University | Yudintceva N.M.,Russian Academy of Sciences | Blinova M.I.,Russian Academy of Sciences | And 9 more authors.
Journal of Biomedical Materials Research - Part A | Year: 2014

Angio- and osteogenesis following the two-stage (TS) implantation of the skin- and bone-integrated pylon seeded with autologous fibroblasts was evaluated. Two consecutive animal substudies were undertaken: intramedullary subcutaneous implantation (15 rabbits) and a TS transcutaneous implantation (12 rabbits). We observed enhanced osseointegrative properties of the intramedullary porous component seeded with fibroblasts induced into osteoblast differentiation, as compared to the untreated porous titanium pylon. The three-phase scintigraphy and subsequent histological analysis showed that the level of osteogenesis was 1.5-fold higher than in the control group, and significantly so (p < 0.05). The biocompatibility was further proved by the absence of inflammatory response or encapsulation and sequestration on the histology assay. Treatment of the transcutaneous component with autologous fibroblasts was associated with nearly a 2-fold decrease in the period required for the ingrowth of dermal and subdermal soft tissues into the implant surface, as compared to the untreated porous titanium component. Direct dermal attachment to the transcutaneous implant prevented superficial and deep periprosthetic infections in rabbits in vivo. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 3033-3048, 2014. © 2013 Wiley Periodicals, Inc.


Blinova M.I.,Russian Academy of Sciences | Yudintzeva N.M.,Russian Academy of Sciences | Nikolaenko N.S.,Russian Academy of Sciences | Potokin I.L.,Russian Academy of Sciences | And 4 more authors.
Cell and Tissue Biology | Year: 2010

The paper presents data on the cultivation of human dermal fibroblasts and rabbit mesenchymal stromal cells on two types of porous titanium implants, i. e., those with irregular pores formed by pressed titanium particles and those with regular pores formed by the cohesion of one-size titanium particles inside the implant. The goal of this study was to determine what type of titanium implant porosity ensured its strongest interaction with cells. Cells were cultivated on implants for 7 days. During this period, they formed a confluent monolayer on the implant surface. Cells grown on titanium implants were monitored by scanning electron microscopy. Fibroblasts interaction with implants depended on the implant porosity structure. On implants with irregular pores cells were more spread. On implants with regular pores fibroblasts enveloped particles and were only occasionally bound with neighboring particles by small outgrowths. There was no tight interaction of particles inside the implant. In implants formed by pressed particles, cells grow not only on surface, but also in the depth of the implant. Thus, we suppose that a tighter interaction of cells with the titanium implant and, supposedly, tissues with the implant in the organism will take place in the variant when the implant structure is formed by pressed titanium particles, i. e., cellular interaction was observed inside the implant. In implants with irregular pores, cells grew both on the surface and in the depth. Thus, cells exhibited more adequate interactions with irregular pore titanium implants in vitro and hopefully the same interaction will be true in tissues after the implantation of the prosthesis into the organism. © 2010 Pleiades Publishing, Ltd.


Pitkin M.,Tufts University | Pilling J.,Michigan Technological University | Raykhtsaum G.,Poly Orth International
Journal of Biomedical Materials Research - Part B Applied Biomaterials | Year: 2012

Composite pylons containing a solid titanium core with drilled holes surrounded by a porous sintered titanium shell have been fabricated and tested in bending along with the raw cores and pylons composed of the porous titanium alone. The new pylons were designed with the concept of enhanced in-growth of bone and skin cells and are intended for direct skeletal attachment of limb prostheses considering requirements for long-lasting anchorage to the residuum bone and a need for a safe skin-implant seal. Load-displacement thresholds were determined after which the integrity of the porous component may be compromised. The composite pylons have a flexural strength and stiffness substantially greater than that of pylons composed of the porous titanium alone. The drilled holes in the solid insert have been shown to have virtually no effect on the flexural strength of the pylon, while meeting a requirement for total permeability of the device for unrestricted cell ingrowth. The predicted strength of the pylons and associated failure modes are in close agreement with those measured. © 2012 WILEY PERIODICALS, INC.


Blinova M.I.,Russian Academy of Sciences | Yudintzeva N.M.,Russian Academy of Sciences | Nikolaenko N.S.,Russian Academy of Sciences | Potokin I.L.,Russian Academy of Sciences | And 4 more authors.
Tsitologiya | Year: 2010

The data on human dermal fibroblasts and rabbit mesenchymal stromal cells cultivation on porous titanium implants are presented in given paper. Two types of implants were used: type 1 - with irregular pores formed by pressed titanium particles and type 2-with regular pores formed by coalescence of one-size titanium particles into implant. The goal of this study was to choose the type of titanium implant porosity which ensures the tightest interaction of titanium implant with surrounding tissue cells after implantation in the body. Cells were cultivated on implants for 7 days and in both cases they formed confluent monolayer on the implants surfaces. That indicated adhesion, migration and proliferation of cells on such implants. Condition of cells cultured on titanium implants was controlled by scanning electron microscopy. The character of fibroblasts interaction with given implants was different depending on porosity type of implants. On implants with irregular pores, the cells were more spread and overlapped the pores spreading over neighbored particles. On implants with regular pores that formed by one-size particles into implant, the fibroblasts covered these particles not overlapping the pores and seldom interacted with neighbored particles by small outgrowths. There was no tight interaction of particles into implant. In implants formed by pressed particles, the cells grew not only on the surface but also in the depth of implant. Thereby, we suppose that more tight interaction of cells with titanium implant and, supposedly, tissues with implant in an organism will take place in the case of implant structure formed by pressed titanium particles.


Grant
Agency: Department of Health and Human Services | Branch: National Institutes of Health | Program: SBIR | Phase: Phase II | Award Amount: 621.05K | Year: 2016

Research efforts of the Poly Orth International demonstrated feasibility of the novel Residuum Integration Prosthetic Technologyandquot to be used in direct skeletal attachment of limb prosthetics The technology includes a Skin and Bone Integrated Pylon SBIP which connects the residuum with external limb prosthesis and can be especially beneficial for patients who can not use traditional socket attachment of the prostheses As histopathology analysis after the study with small animals has demonstrated the SBIP is integrated not only with the residual bone but also with the residuumandapos s skin minimizing the risk of infection and secondary trauma The investigators developed an optimal porous titanium matrix and design of the Skin and Bone Integrated Pylon to maximize the ingrowth of bone and skin cells of the residuum to the SBIP without compromising strength of the implanted device The technological study on the process of manufacturing of the composite porous structure enforced with a permeable internal frame Parameters of the SBIPandapos s specification are protected by the US Patent During the proposed translational pre clinical study the animal trials will be conducted according to the FDA requirements to ensure smooth translation to the trials in humans It will include large animals and the implantation period up to months Inadequate prosthetic rehabilitation after limb amputation is a serious problem relevant to public health The public value of solving this serious problem is much more elevated when a country is at war Providing US amputees with infection safe and long lasting direct skeletal attachment of prostheses will improve the quality of their lives and eliminate the costs associated with the multiple fabrications adjustments and frequent failures of the prosthetic sockets

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